• Animal Bioscience
• /
• v.35 no.7
• /
• pp.975-988
• /
• 2022

Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research. Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA-DEmRNA pairs with a Pearson correlation coefficient greater than 0.99. Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research.

• Journal of the Korea Institute of Building Construction
• /
• v.13 no.6
• /
• pp.609-618
• /
• 2013

The unit modular construction system is a building technique in which unit modules are manufactured at a factory and then assembled at the construction site. It has many benefits, including reduction of the construction period and improvement in quality. For this reason, it is utilized for various purposes in Japan and England. While it has been introduced in Korea, there have been few Korean studies conducted on the unit modular system. In particular, little research has been done on the method of safely fixating the unit modules to a truck. Therefore, this study reviewed the fixation methods of unit modules for transport, analyzed the problems, and designed a fixation device for unit modules. In addition, a device for the fixation of unit modules to a truck was developed, and a structural simulation was implemented for a safety test by considering the maximum stress generated during the transport of the unit modules fixed on a truck. When the device for the fixation of unit modules is manufactured based on the results of the structural simulation done in this study, it is expected to aid the development of a more practical fixation device for unit modules.

• The Journal of Korean Physical Therapy
• /
• v.13 no.3
• /
• pp.719-726
• /
• 2001

Transdermal drug delivery offers various advantages over conventional drug delivery systems, such as avoidance gastrointestinal degradation and hepatic first-pass effect. encourages patient compliance. and possible sustained release of drugs. However, transdermal transport of drugs is low permeability of the stratum corneum, the superficial layer of the skin. Many physicochemical and biological factors influencing transdermal transport is described together with the corresponding experimental and clinical results. Phonophoresis is medical treatment with drugs introduced into the skin by ultrasound energy. Enhanced drug penetration is through to result from the biophysical alterations of skin structure by ultrasound waves. The frequency used for phonophoresis is usually from 20 kHz to 15MHz. Phonophoresis can be categorized in to three ranges: low-frequency range(below 1 MHz). therapeutic frequency range(1 to 3MHz), and high-frequency range(above 3 MHz). The depth of penetration of ultrasound into skin is inversely proportional to the frequency. Cavitation may cause mechanical stress. temperature elevation, or enhanced chemical reactivity causing drug transport. One theory is that ultrasound affects the permeation of the stratum corneum lipid structure as the limiting step in permeating through the skin. The range of indications for phonophoresis is wide. Aspecific classification of the range of indications is obtained by classification of pathological conditions. The continuous research is needed for many interesting issucs of phonophoretic transdermal delivory in new future.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.6
• /
• pp.874-878
• /
• 2013

Unlike Europe (particularly, Italy and Spain), where a number of studies have been conducted on the stressful effects of transport on rabbit welfare, few studies have been conducted on transportation of rabbits under hot, humid tropical conditions experienced in countries like Malaysia. We studied the effects of transportation in hot humid tropical conditions of Malaysia on physiometabolic changes in New Zealand white rabbits. Eighty experimental animals were divided into two groups of 40 bucks each and transported for either 3 or 1 h. Transportation caused a significant upsurge of aspartate aminotransferase, alanine aminotransferase and creatine kinase activities (p<0.001) though did not significantly affect lactate dehydrogenase (LDH) activity (p = 0.0706). Both transportation periods caused elevation in plasma glucose levels, lactic acidosis and dehydration as evidenced through elevated packed cell volume and plasma protein concentration. It was concluded that regardless of the duration, transport of rabbits under hot humid tropical conditions, resulted in heat distress since the rabbits showed hyperglycemia, hypercalcemia, lactacidemia, lymphocytopenia, dehydration and increase in blood enzyme activities.

• Corrosion Science and Technology
• /
• v.16 no.4
• /
• pp.209-225
• /
• 2017

Development of high strength steel requires proper understanding of hydrogen behavior since the higher the steel strength the greater the susceptibility of hydrogen assisted cracking. This paper provides a brief but broad overview on hydrogen entry and transport behavior of high-strength ferritic steels. First of all, hydrogen absorption, diffusion and trapping mechanism of the steels are briefly introduced. Secondly, several experimental methods for analyzing the physical/chemical nature of hydrogen uptake and transport in the steels are reviewed. Among the methods, electrochemical permeation technique utilized widely for evaluating the hydrogen diffusion and trapping behavior in metals and alloys is mainly discussed. Moreover, a modified permeation technique accommodating the externally applied load and its application to a variety of steels are intensively explored. Indeed, successful utilization of the modified permeation technique equipped with a constant load testing device leads to significant academic progress on the hydrogen assisted cracking (HAC) phenomenon of the steels. In order to show how the external and/or residual stress affects mechanical instability of steel due to hydrogen ingress, the relationship among the microstructure, hydrogen permeation, and HAC susceptibility is briefly introduced.

• International Journal of Aerospace System Engineering
• /
• v.11 no.1
• /
• pp.1-3
• /
• 2024

Around the world, fossil fuel energy is being replaced with renewable energy due to environmental problems and sharp price increases. Many countries are making a change in the direction of moving toward eco-friendliness by reducing carbon emissions. Among renewable energies, the wind energy is eco-friendly because it produces electricity by wind power without carbon emissions, and it attracts attention worldwide as a great alternative to the exhausted fuel energy. To improve the efficiency of wind turbines, large and extra-large wind turbines have been developed all over the world by increasing install and diameter. These wind turbines have difficulty in transport after manufacture because of their size and height. Since the height of wind turbine blades is higher than the existing tunnel height, it is impossible to transport them. In this study, therefore, a 5 MW class large blade was separated for transport easiness as wind power generators became larger globally. Aerodynamic design and analysis was carried out for the blade. After performing structural design and analysis with the model designed, the stress concentration of the analyzed model and the various factors for consideration when separating were considered to conduct the study of selecting the optimal blade separation positions.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.194-194
• /
• 2017

Camelina (Camelina sativa L.) is a potential bio-energy crop that has short life cycle about 90 days and contains high amount of unsaturated fatty acid which is adequate to bio-diesel production. Enhancing environmental stress tolerance is a main issue to increase not only crop productivity but also big mass production. CsRCI2s (Rare Cold Inducible 2) are cold and salt stress related protein that localized at plasma membrane (PM) and assume to be membrane potential regulation factor. These proteins can be divide into C-terminal tail (CsRCI2D/E/F/G) or no-tail group (CsRCI2A/B/C/H). However, function of CsRCI2s are less understood. In this study, physiological responses and functional characterization of CsRCI2s of Camelina under salt stress were analyzed. Full-length CsRCI2s (A/B/E/F) and CsPIP2;1 sequences were confirmed from Camelina genome browser. Physiological investigations were carried out using one- or four-week-old Camelina under NaCl stress with dose and time dependent manner. Transcriptional changes of CsRCI2A/B/E/F and CsPIP2;1 were determined using qRT-PCR in one-week-old Camelina seedlings treated with NaCl. Translational changes of CsRCI2E and CsPIP2;1 were confirmed with western-blot using the antibodies. Water transport activity and membrane potential measurement were observed by cRNA injected Xenopus laevis oocyte. As results, root growth rate and physiological parameters such as stomatal conductance, chlorophyll fluorescence, and electrolyte leakage showed significant inhibition in 100 and 150 mM NaCl. Transcriptional level of CsPIP2;1 did not changed but CsRCI2s were significantly increased by NaCl concentration, however, no-tail type CsRCI2A and CsRCI2B increased earlier than tail type CsRCI2E and CsRCI2F. Translational changes of CsPIP2;1 was constitutively maintained under NaCl stress. But, accumulation of CsRCI2E significantly increased by NaCl stress. CsPIP2;1 and CsRCI2A/B/E/F co-expressed Xenopus laevis oocyte showed decreased water transport activity as 61.84, 60.30, 62.91 and 76.51 % at CsRCI2A, CsRCI2B, CsRCI2E and CsRCI2F co-expression when compare with single expression of CsPIP2;1, respectively. Moreover, oocyte membrane potential was significantly hyperpolarized by co-expression of CsRCI2s. However, higher hyperpolarized level was observed in tail-type CsRCI2E and CsRCI2F than others, especially, CsRCI2E showed highest level. It means transport of $Na^+$ ion into cell is negatively regulated by expression of CsRCI2s, and, function of C-terminal tail is might be related with $Na^+$ ion influx. In conclusion, accumulation of NaCl-induced CsRCI2 proteins are related with $Na^+$ ion exclusion and prevent water loss by CsPIP2;1 under NaCl stress.

• Journal of Life Science
• /
• v.26 no.4
• /
• pp.490-495
• /
• 2016

Brefeldin A (BFA), a lactone antibiotic isolated from the fungus Eupenicillium brefeldianum, inhibits the transport of secreted and membrane proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. BFA disrupts Golgi function, the accumulation of unfolded proteins in ER, and the induction of ER stress. Prolonged ER stress induces apoptosis at least in part through the transcription factor C/EBP (CCAAT/enhancer binding protein) homologous protein (CHOP),which is activated by the unfolded protein response (UPR). In this paper, we demonstrate that BFA-induced endoplasmic reticulum stress leads to different CHOP expression in primary astrocyte cells and C6 glioma cells. BFA induced lower CHOP expression levels in primary astrocyte cells than in C6 glioma cells; however, other ER stress inducers (thapsigargin and tunicamycin) resulted in similar expression patterns in these two cell types. Interestingly, the three different ER stress inducers (BFA, thapsigargin, and tunicamycin) induced similar levels of CHOP mRNA expression in primary astrocyte cells. The ubiquitin-proteasome inhibitor MG132 also markedly up-regulated the BFA-mediated CHOP protein expression in primary astrocyte cells. BFA also induced higher proteasome activity in primary astrocyte cells than in C6 glioma cells. Taken together, our results suggest that higher proteasomal activity might down-regulate BFA-induced CHOP expression in primary astrocyte cells.

• Journal of Korean Society of Water and Wastewater
• /
• v.18 no.1
• /
• pp.5-14
• /
• 2004

A feasibility of using Particle Entrainment Simulator (PES) to evaluate model variables describing sediment entrainment in a river system was investigated. PES in a laboratory was utilized to simulate the sediment resuspension phenomenon in the river and the subsequent relationship between shear rate and sediment entrainment was developed. The total suspended solids (TSS) data from PES was incorporated into statistical models in an effort to describe behaviors of net particle movement in the river. PES was found to be adequate for simulating particle entrainment phenomenon in a river system. Statistical analysis was used to assess propriety of PES data for predictive purposes. The results showed good relationships between PES results and system variables, such as average stream velocity and net particle movement.

• Proceedings of the Korean Nuclear Society Conference
• /
• 1996.05b
• /
• pp.227-232
• /
• 1996

The one-third calibrating relation by steady solution can cause large error when applied to an unsteady flow with large amplitude waves. Extended calibrating method, which can treat the normal convective contribution, is developed. The normal mass convective term is included into the 2-D mass transport equation by means of rms value and random function. The unknown shear rate is numerically determined by solving the 2-D mass transport equation inversely. This recovery method which predicts the unknown shear rate is constructed. It is found that it works very well without distortion. The inclusion of the normal convective term has a negligible effect on the mass transfer coefficient.