• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1782-1786
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• 2015

A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.

• Mycobiology
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• 제43권3호
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• pp.366-370
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• 2015

Acanthopanax divaricatus, a member of the Araliaceae family, has been used as an invigorant in traditional Korean medicine. During disease monitoring, a stem with small, irregular, brown lesions was sampled at a farm in Cheonan in 2011. The symptoms seen were sunken cankers and reddish-brown needles on the infected twig. The isolated fungal colonies were whitish, having crenated edges and aerial mycelium on the surface, and with black gregarious fruiting bodies. The reverse plate was creamy white. Conidia were $17{\sim}22{\times}3.5{\sim}4.2{\mu}m$, fusiform, 4-septate, and straight to slightly curved. The nucleotide sequence of the partial translation elongation factor 1 alpha gene of the fungal isolate, shares 99% sequence identity with that of known Pestalotiopsis ellipsospora. Based on the results of the morphological and molecular analyses, the fungal isolate was identified as P. ellipsospora. In Korea, this is the first report of canker on A. divaricatus.

• 한국균학회지
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• 제41권1호
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• pp.52-55
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• 2013

Phoma glomerata는 식물 잎이나 열매에 병을 일으키는 식물병원균으로 알려져 있다. 국내에서는 아직 피해사례가 없기 때문에 P. glomerata는 국내의 식물검역균으로 관리되고 있다. 본 연구는 국내에 들어오는 목재나 과일에 P. glomerata를 검출할 수 있는 방법 개발코자 수행되었다. Phoma 균주들의 translation elongation factor 1 alpha 유전자 염기서열에 기초하여 P. glomerata 특이적 PCR 프라이머를 디자인 하였고 그 특이성을 검정하였다. PCR 수행 결과 P. glomerata에서만 170 bp 크기의 밴드가 증폭되었고, 다른 비교 균주에서는 밴드가 증폭되지 않았다. 검출 감도를 평가하기 위해 기존 PCR방법과 real time PCR 방법을 이용하여 실험한 결과 최소 10 pg과 1 pg까지 각각 검출할 수 있었다. 본 연구결과는 디자인된 PCR 프라이머가 P. glomerata를 특이적으로 검출하는데 유용할 것임을 보여준다.

• 한국균학회지
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• 제46권4호
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• pp.495-503
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• 2018

2016년 4월부터 6월까지 표고 원목재배 임가 4곳과 표고 톱밥재배 임가 1곳의 재배사 내 실내 공기에서 진균을 포집 및 분리 동정하였다. 총 6개의 국내 미기록 진균(Cenangium acuum, Periconia macrospinosa, Neopestalotiopsissurinamensis, Metarhiziummarquandii, Trichoderma petersenii, Trichoderma paratroviride)을 분리하였으며 이들 균류에 대하여 형태학적 특성 및 internal transcribed spacer rDNA region, large subunitregion, ${\beta}-tubulin$ 또는 translation elongation factor $1{\alpha}$유전자 염기서열에 기반하여 계통학적 분석 결과를 기술하였다.

• Mycobiology
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• 제34권2호
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• pp.45-55
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• 2006

Although Fursarium oxysporum causes diseases in economically important plant hosts, identification of F. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. To resolve these complexity, we evaluated genetic relationship of nine formae speciales of F. oxysporum with random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and translation elongation factor-l alpha ($EF-1{\alpha}$) gene. In addition, the correlation between mycotoxin content of fusaric acid and isolates based on molecular marker data was evaluated using the modified Mantel's test. According to these result, these fusaric acid-producing strains could not identify clearly, and independent of geographic locations and host specificities. However, in the identification of F. oxysporum formae speciales, especially, AFLP analysis showed a higher discriminatory power than that of a the RAPD and $EF-1{\alpha}$ analyses, all three techniques were able to detect genetic variability among F. oxysporum formae speciales in this study.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.117.2-117
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• 2003

The importance and diversity of the genus Stemphylium highlights the need for accurate identification of species. However, many Stemphylium isolates have been misidentified due to the use of spore size as the only identifying character. Molecular phylogenetic analyses were performed on fifty-four isolates covering 9 Stemphylium species collected in Korea. Phylogenetic analysis of the translation elongation factor -1 alpha (EF-1) and the calmodulin gene sequence data showed that Stemphylium species were segregated into seven distinct groups, most of w hichcorrelated with species identified by morphology. Analysis of EF-1 in particular was useful for establishing well- supported relationships among the species of Stemphylium.

• 식물병연구
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• 제21권1호
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• pp.24-26
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• 2015

In April 2014, seedlings of Fallopia multiflora showing wilt symptom were first found at a greenhouse in Punggi-eup, Yeongju-si, Korea. A Fusarium-like fungus was isolated from the wilted plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor 1-${\alpha}$. The fungus isolated from the diseased plant was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of Fusarium wilt occurring on Fallopia multiflora in the world.

• Current Research on Agriculture and Life Sciences
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• 제31권4호
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• pp.245-249
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• 2013

In 2008, leaf blight symptoms were observed on several Chinese chive farms in Sangju. The Pathogenicity of the isolate was confirmed by artificial inoculation, where the pathogen exhibited a strong pathogenicity toward healthy plants. Morphological classification identified the isolate as from the Fusarium genus. For further analysis, PCR and phylogenetic classification were performed with ITS region and 28S rRNA gene which are commonly used for fungal identification. However, the results provided a poor resolution. To solve this problem, we analyzed translation elongation factor 1-alpha (TEF-$1{\alpha}$) gene. The analyzed results using TEF-$1{\alpha}$ gene indicated that the isolate was F. proliferatum. Therefore, it is assumed that TEF-$1{\alpha}$ gene is important when Fusarium sp. was identified using molecular classification method.

• 식물병연구
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• 제26권1호
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• pp.48-52
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• 2020

2019년 7월 안동지역 노지 재배지에서 복숭아에 과실 썩음병 피해가 발생하였다. 피해 과실에서는 병이 진전됨에 따라, 썩음증상과 함께, 흰색과 자주색을 띄는 균사 및 포자가 관찰되었다. 병원균을 순수 분리한 뒤, 건전한 복숭아 과일에 접종하였을 때 동일한 과실 썩음 증상을 유도하였다. 분리된 병원균의 internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF) 그리고 β-tubulin (β-TUB) sequence 분석을 통해 Fusarium avenaceum으로 동정되었다. 따라서, 이 증상을 Fusarium avenaceum에 의한 "복숭아 과실 썩음병"으로 명명하고자 한다. 분리된 균주는 농촌진흥청 국립농업과학원 미생물 은행[Korean Agricultural Culture Collection(KACC)]에 기탁되었다(KACC accession number 48936).

• 식물병연구
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• 제17권1호
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• pp.82-85
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• 2011

2009년 전남 해남 지역의 논에서 벼 키다리병과 유사한 병징을 나타내는 피를 채집하여 병원균을 분리하였다. 이병 식물체는 줄기가 고사하여 하얗게 말라 죽고, 줄기 표면에 흰가루 모양의 포자가 형성되었다. 분리균은 균학적 특성과 분자생물학적 특성에 따라 Fusarium fujikuroi로 동정되었다. Translation elongation factor 1-alpha 유전자의 염기서열 분석 결과, GenBank에 등록된 F. fujikuroi와 상동성을 나타내었다. 벼와 피를 대상으로 침지접종에 의해 병원성을 확인하였으며, 이는 피가 벼 키다리병의 전염원으로서의 역할을 할 수 있음을 의미한다. 따라서 벼 키다리병의 방제와 건전종자의 채종을 위해서는 논에 발생한 이병식물체뿐 아니라 전염원으로 작용할 수 있는 피를 제거해야 할 것으로 생각된다.