• Korean Journal of Microbiology
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• v.42 no.4
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• pp.271-276
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• 2006

Previously we demonstrated that virus-inducible stress protein (VISP) is induced in fish cells by the infection of a fish rhabdovirus. In this paper, we investigated the subcellular localization of the VISP and determined the region of VISP responsible for the subcellular localization. The CHSE-214 cells were stained with monoclonal antibody raised against VISP and observed with confocal microscope to detect the endogenous VISP. The results showed that the VISP localizes to the perinuclear region as spots. A plasmid expressing VISP fused to enhanced green fluorescent protein (EGFP) was constructed. The transient expression of full-length VISP fused to EGFP in CHSE-214 cells confirmed the spot formation of the VISP at perinuclear region. To determine the region responsible for the perinuclear localization of the VISP, we constructed a series of deletion mutants and, by using these deletion mutants, we found that C-terminal region of the VISP (aa 612-710) is essential for the perinuclear distribution of VISP and that this region contained nuclear receptor binding motif (691-TLTSLLL-697). Our results suggest that VISP localizes to the perinuclear region and C-terminal regions are important for this localization. Further studies on the role of the perinuclear localization of VISP in IHNV growth mali reveal the novel mechanism of IHNV pathogenecity.

• Molecules and Cells
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• v.27 no.1
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• pp.113-118
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• 2009

In this study, we have shown the transcriptional regulation of the human GD3 synthase (hST8Sia I) induced by valproic acid (VPA) in human neuroblastoma SK-N-BE(2)-C cells. To elucidate the mechanism underlying the regulation of hST8Sia I gene expression in VPA-stimulated SK-N-BE(2)-C cells, we characterized the promoter region of the hST8Sia I gene. Functional analysis of the 5'-flanking region of the hST8Sia I gene by the transient expression method showed that the -1146 to -646 region, which contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-${\kappa}B$, functions as the VPA-inducible promoter of hST8Sia I in SK-N-BE(2)-C cells. Site-directed mutagenesis and electrophoretic mobility shift assay indicated that the NF-${\kappa}B$ binding site at -731 to -722 was crucial for the VPA-induced expression of hST8Sia I in SK-N-BE(2)-C cells. In addition, the transcriptional activity of hST8Sia I induced by VPA in SK-N-BE(2)-C cells was strongly inhibited by SP600125, which is a c-Jun N-terminal kinase (JNK) inhibitor, and $G{\ddot{O}}6976$, which is a protein kinase C (PKC) inhibitor, as determined by RT-PCR (reverse transcription-polymerase chain reaction) and luciferase assays. These results suggest that VPA markedly modulated transcriptional regulation of hST8Sia I gene expression through PKC/JNK signal pathways in SK-N-BE(2)-C cells.

• Journal of Korea Water Resources Association
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• v.51 no.11
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• pp.971-976
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• 2018

An accurate analysis of pipeline transient is important for proper management and operation of a water distribution systems. The computational accuracy and its cost are two distinct components for unsteady flow analysis model, which can be strength and weakness of three-dimensional model and one-dimensional model, respectively. In this study, we used two-dimensional unsteady flow model with Five-Region Turbulence model (FRTM) with the implementation of interaction between liquid and air Since FRTM has an empirical component to be determined, we explored the response feature of two-dimensional flow model. The relationship between friction behaviour and the variation of undetermined parameter was configured through the comparison between numerical simulations and experimental results.

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.3
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• pp.222-226
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• 2007

In this paper we composed the dual fuzzy rules using each region of specific points and $e-{\Delta}e$ phase plane In order to make dual fuzzy rule base. We composed the fuzzy control rules which can decrease rise time, delay time, maximum overshoot than basic fuzzy control rules. proposed method is alternately use at specific points of $e-{\Delta}e$ phase plane with two fuzzy control rules that is one control rule occruing the steady state error in transient region and another fuzzy control rule use to decrease the steady state error and rapidly converge at the convergence region. Also, two fuzzy control rules in the $e-{\Delta}e$ phase plane decide the change time according to response characteristics of plants. In order to confirm thef proposed algorithm. As the results of experiments through the hydraulic servo motor control system with a DSP processor, We verified that proposed dual fuzzy control rules get the good response compare with the basic fuzzy control rule.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.261-282
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• 1987

Plants store a significant amount of their nitrogen, sulfur and carbon reserves as storage proteins in seed tissues. The major proteins present in rice seeds are the glutelins. Glutelins are initially synthesized at 4-6 days postanthesis and deposited into protein bodies via Golgi apparatus. Based on nucleic acid sequences and Southern blot analysis, the three isolated glutelin genomic clones were representative members of three gene subfamilies each containing 5 to 8 copies. A comparison of DNA sequences displayed by relevant regions of these genomic clones showed that two subfamilies, represented by clones, Gt1 and Gt2, were closely, related and probably evolved by more recent gene duplication events. The 5' flanking and coding sequences of Gt1 and Gt2 displayed at least 87% homolgy. In contrast, Gt3 showed little or no homolgy in the 5' flanking sequences upstream of the putative CAAT boxes and exhibited significant divergence in all other portions of the gene. Conserved sequences in the 5' flanking regions of these genes were identified and discussed in light of their potential regulatory role. The derived primary sequences of all three glutelin genomic clones showed significant homology to the legume 11S storage proteins indicating a common gene origin. A comparison of the derived glutelin primary sequences showed that mutations were clustered in three peptide regions. One peptide region corresponded to the highly rautable hypervariable region of legume peptide region of legume 11S storage proteins, a potential target area for protein modification. Expression studies indicated that glutelin mRNA transcripts are differentially accumulated during endosperm development. Promoterss of Gt2 and Gt3 were functional as they direct transient expression of chloramphenicol acetyltransferase in cultured plant cell.

• KIEE International Transactions on Electrophysics and Applications
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• v.4C no.5
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• pp.236-240
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• 2004

In this paper we examine the reflection characteristics of dielectric microstrip lines with open-ended termination containing an optically induced plasma region, which are analyzed by the assumption that the plasma is distributed homogeneously in laser illumination. The characteristics impedances resulting from the presence of plasma are evaluated by the transmission line model. To estimate theoretically the characteristic response of identical systems in the time domain, the Fourier transformation method is evaluated. The reflection characteristics of time and frequency response in microwave systems have been calculated using an equivalent circuit model.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.49.3-50
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• 2015

Synchrotron radiation of relativistic electrons is an important radiation mechanism in many astrophysical sources. In the sources where the synchrotron cooling timescale is shorter than the dynamical timescale, electrons are cooled down below the minimum injection energy. It has been believed that such fast-cooling electrons have a power-law distribution in energy with an index -2, and their synchrotron radiation has a photon spectral index -1.5. On the other hand, in a transient expanding astrophysical source, such as a gamma-ray burst (GRB), the magnetic field strength in the emission region continuously decreases with radius. Here we study such a system, and find that in a certain parameter regime, the fast-cooling electrons can have a harder energy spectrum. We apply this new physical regime to GRBs, and suggest that the GRB prompt emission spectra whose low-energy photon spectral index has a typical value -1 could be due to synchrotron radiation in this moderately fast-cooling regime.

• Journal of ILASS-Korea
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• v.20 no.2
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• pp.101-106
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• 2015

Gas leakage and dispersion in the underground LNG power plant can lead to serious fire and explosion accident. In this study, computational fluid dynamics simulation was applied to model the dynamic process of gas leakage and dispersion phenomena in a closed space. To analyze the risk assessment factor, such as the flammable volume ratio, transient simulations were carried out for different scenarios. The simulation results visualized the gas distribution with time in the closed space. The flammable volume ratio was introduced for quantitative analysis the fire/explosion probability.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.3
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• pp.320-329
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• 1999

An experimental study has been performed to investigate the heat transfer characteristics of impinging jets with corrugated nozzle and wake generation plate. Three different shapes of corrugated nozzle and five different shapes of wake generation plate were tested to improve the heat transfer characteristics of impinging jet. Heat transfer coefficients were obtained by using transient method based on the liquid crystal thermography. The effects of corrugated nozzle and wake generation plate on the heat transfer characteristics of impinging jets were discussed in detail. The results showed that both the corrugated nozzle and the wake generation plate improved the heat transfer characteristics of impinging jet. Especially, heat transfer coefficients around stagnation region of impinging jets were highly increased.

• Journal of Aerospace System Engineering
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• v.6 no.4
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• pp.24-28
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• 2012

In this paper, 2-D experiment and steady-state computational fluid analysis were conducted for measuring the hear transfer coefficient of pintle type controllable thruster in high pressure and temperature. In case of 2-D experiment, transient liquid crystal technique was used for measuring heat transfer coefficient for the 2-D pintle model. The experimental result was used to validate the CFD result. The CFD results well predicted the heat transfer coefficient on the pintle surface except the nozzle downstream region, where the results by CFD was higher than experimental results. The CFD results were also compared with the result by Bartz equation and the it was shown that the Bartz equation overestimated the heat transfer coefficient on the nozzle throat as much as 80%.