• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1383-1391
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• 2016

Bovine embryonic stem cells have potential for use in research, such as transgenic cattle generation and the study of developmental gene regulation. The Nanog may play a critical role in maintenance of the undifferentiated state of embryonic stem cells in the bovine, as in murine and human. Nevertheless, efforts to study the bovine Nanog for pluripotency-maintaining factors have been insufficient. In this study, in order to understand the mechanisms of transcriptional regulation of the bovine Nanog, the 5'-flanking region of the Nanog was isolated from ear cells of Hanwoo. Results of transient transfection using a luciferase reporter gene under the control of serially deleted 5'-flanking sequences revealed that the -134 to -19 region contained the positive regulatory sequences for the transcription of the bovine Nanog. Results from mutagenesis studies demonstrated that the Sp1-binding site that is located in the proximal promoter region plays an important role in transcriptional activity of the bovine Nanog promoter. The electrophoretic mobility shift assay with the Sp1 specific antibody confirmed the specific binding of Sp1 transcription factor to this site. In addition, significant inhibition of Nanog promoter activity by the Sp1 mutant was observed in murine embryonic stem cells. Furthermore, chromatin-immunoprecipitation assay with the Sp1 specific antibody confirmed the specific binding of Sp1 transcription factor to this site. These results suggest that Sp1 is an essential regulatory factor for bovine Nanog transcriptional activity.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.8
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• pp.56-63
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• 2020

The heat transfer phenomenon in the vicinity of the irradiated region of a focused laser beam of a DMT process greatly affects both the deposition characteristics of powders on a substrate and the properties of the deposited region. The goal of this paper is to investigate the heat transfer characteristics of a single bead deposition of Inconel 718 powders on S45C structural steel using a laser-aided direct metal tooling (DMT) process. The finite element analysis (FEA) model with a Gaussian volumetric heat flux is developed to simulate a three-dimensional transient heat transfer phenomenon. The cross-section of the bead for the FEA is estimated with an equivalent area method using experimental results. Through the comparison of the results of the experiments and those of the analysis, the effective beam radius of the bottom region of the volumetric heat flux and the efficiency of the heat flux model for different powers and travel speeds of the laser are predicted. From the results of the FEA, the influence of the power and the travel speed of the laser on the creation of a steady-state heat transfer region and the formation of the heat-affected zone (HAZ) in the substrate are investigated.

• Journal of the Semiconductor & Display Technology
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• v.15 no.2
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• pp.26-31
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• 2016

The Townsend to glow discharge mode transition was investigated in the dielectric barrier discharge (DBD) helium plasma source which was powered by 20 kHz / $4.5 kV_{rms}$ high voltage at atmospheric pressure. The spatial profile of the electric field strength at each modes was measured by using the intensity ratio method of two helium emission lines (667.8 nm ($3^1D{\rightarrow}2^1P$) and 728.1 nm ($3^1S{\rightarrow}2^1P$)) and the Stark effect. ICCD images were analyzed with consideration for the electric field property. The Townsend discharge (TD) mode at the initial stage of breakdown has the light emission region located in the vicinity of the anode. The electric field of the light emitting region is close to the applied field in the system. Immediately, the light emitting region moves to the cathode and the discharge transits to the glow discharge (GD) mode. This mode transition can be understood with the ionization wave propagation. The electric field of the emitting region of GD near cathode is higher than that of TD near anode because of the cathode fall formation. This observation may apply to designing a DBD process system and to analysis of the process treatment results.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.3407-3412
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• 2007

The performance characteristics of the polymer electrolyte fuel cells (PEFCs) were investigated under various humidification conditions at steady-state and transient conditions. The PEFC studied in this study was characterized by I-V curves in potentiostatic mode. The I-V curves representing steady-state performance were obtained from OCV to 0.25V, and the dynamic performance responses were obtained at some points of voltages. The anodic external humidification was applied and the humidity was controlled from 20% to 100%. The effects of relative humidity of hydrogen were measured with the dry air at the cathode. At high voltage region, the performance at high temperature was higher, but at low voltage region, low temperature condition showed the higher performance. The dynamic responses were observed at the instant when the voltage of the PEFC was changed. It was observed that the performance reached steady-state earlier with the increase of temperature.

• Toxicological Research
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• v.16 no.2
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• pp.95-100
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• 2000

In previous study, both constitutive expression and 3-methylcholanthrene (3MC)-mediated elevation of CYP1A2 mRNA were demonstrated in human hepatoma HepG2 cells by reverse transcription-polymerase chain reaction (RT-PCR), suggesting that HepG2 cells would be appropriate for the study of human CYP1A2 regulation(Chung and Bresnick, 1994). Further studies were conducted to determine the basis of this induction phenomenon that is observed in HepG2 cells. Since CYP1A1 gene, another polycyclic hydrocarbon(PH)-inducible gene, is regulated by PHs through their interactions via receptors with cis-elements, the 5'-flanking region of human CYP 1A2 gene was analyzed to search such responsive elements. The promoter activity of various lengths of CYP1A2 gene sequence (-3203/+58bp) was measured in transiently-transfected HepG2 cells by fusion constructs containing the CAT, hGH or luciferase genes as a reporter. This region of the CYP1A2 gene, although containing a XRE, was only weakly responsive (less than 2 fold induction) to 10 nM of TCDD or 1 $\mu$M 3 MC treatment. This small enhancement of promoter activity is inconsistent with the previous observation, i.e., 12 to 14 fold-enhanced CYP1A2 mRNA from 1 $\mu$M 3 MC treated HepG2 cells, suggesting that additional mechanisms would exist for PH-mediated induction of CYP1A2 in these cells.

• Journal of ILASS-Korea
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• v.20 no.2
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• pp.114-120
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• 2015

Effects of injection conditions on a pintle injector which is proper to recent liquid rocket engines requiring low cost, low weight, high efficiency and reusability were studied. The pintle injector with a typical moving pintle was used for atmospheric experiment using water and air. Injection pressures of water were considered 0.5 and 1.0 bar, 0.1 to 1.0 bar for injection pressures of air and 0.2 to 1.0 mm for pintle opening distance. Sauter mean diameters (SMD) of spray was measured at 50 mm distance from a pintle tip and SMD was treated as a representative parameter in this study. As a result, because of shape characteristics of the pintle injector, there was a transient region between the pintle opening distances of 0.6 and 0.7 mm and this region affected to mass flow rates and SMDs. Also, Reynolds numbers for gas, Weber numbers and momentum ratios were adopted as major non-dimensional paramters and the momentum ratio has strong correlation with SMD.

• Journal of Electrical Engineering and Technology
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• v.12 no.4
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• pp.1495-1502
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• 2017

The Theory of operation of switched reluctance motors (SRM) depends on the reluctance torque, where energy is transferred to stator winding only. Although its construction is simple, the electrical design is complex, due to the switching configuration needed to deliver power to stator coils. However, because of the nonlinearly of magnetic circuit, SRM has torque ripple. This paper proposes a new strategy to drive SRM from a single-phase AC supply. Each stator winding is connected to AC-DC or AC-AC converters, which is called branch. All branches are connected in parallel to a single-phase AC supply. A shaft encoder allows current production in stator winding during the positive torque production region and terminates it during the negative torque production region. A magnetic flux is produced between stator poles when current is supplied from AC supply to stator coil and repeats many cycles as long as the rate of change of stator inductance is positive. Different possibilities for the configurations of AC-AC or AC-DC converters are introduced to drive SRM from the single-phase AC supply. A case study is presented for a SRM fed from AC supply through semi-controlled AC-DC converter is presented. A simulation model is introduced and verified by experimental rig for two-phase SRM.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.17 no.5
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• pp.460-471
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• 1992

By setting a new model to describe the time-discontinuous operation of PLL loop which used tri-state and sample-hold method, the stability analysis of nonlinear PLL has been performed in z-domain and the state equations for the transient response has been introduced. Until now, the lin-ear analysis by approximation of time-discontinuous to time-continuous operation had not found then stable region of time-discontinuous digital PLL exactly. However, the analysis In z-domain by the new model has been found the unstable region where the time-continuous analysis had have not. 1'herefore the limit of loop coefficient has been computed to design digital PLL optimally.

• Journal of the Society of Naval Architects of Korea
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• v.50 no.6
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• pp.450-457
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• 2013

For the design guide of a vessel operating in cold region, numerical analysis was carried out to evaluate the weather-tight door which installed the heating cables by using ANSYS 13.0 Transient Thermal. The numerical analysis was performed by considering Advection-Diffusion equation. This study based on the experimental results of 'A study on Anti-Icing Technique for Weather-Tight Door of Ice-Strengthened Vessels'(Jeong, et al., 2011a) in KIOST. For validation of the numerical analysis results, the cold chamber experimental data measured by the heat sensors in certain location of the weather-tight door was used. The external environmental temperature which varies from $5^{\circ}C$ to $-55^{\circ}C$ was considered in numerical analysis. Also three different heating cables which have the heat capacity of 33W/m, 45W/m and 66W/m were adapted for the design parameters to be the most efficient and guidelines for anti-icing design of the weather tight door.

• Journal of computational fluids engineering
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• v.11 no.2 s.33
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• pp.8-18
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• 2006

A CFD benchmark calculation for a steam blowdown test was performed for 30 seconds to develop the methodology of numerical analysis for the thermal mixing between steam and subcooled water. In the CFD analysis, the grid model simulating the sparger and the IRWST pool were developed by the axisymmetric condition and then the steam condensation phenomena by a direct contact was modelled by the so-called condensation region model. Thermal mixing phenomenon in the subcooled water tank was treated as an incompressible flow, a free surface flow between the air and the water, a turbulent flow, and a buoyancy flow. The comparison of the CFD results with the test data showed a good agreement as a whole, but a small temperature difference was locally found at some locations. The commercial CFD code of CFX4.4 together with the condensation region model can simulate the thermal mixing behavior reasonably well when a sufficient number of mesh distribution and a proper numerical method are adopted.