• Archives of Plastic Surgery
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• v.40 no.6
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• pp.676-686
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• 2013

Background To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-${\beta}$1 (LTGF) into an electrospun poly(L-lactide) scaffold. Methods The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats. Results Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen. Conclusions We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering.

• The Journal of the Korea Contents Association
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• v.8 no.11
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• pp.306-322
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• 2008

The purpose of this study is to inquire into the effect of characteristics of public enterprise CEOs' leadership (transforming leadership and transactional leadership) on the leader trust and organizational effectiveness of organizational members. A survey was made on 1,124 persons being in the service of 14 marketable and quasimarketable public enterprises. The results of this study were as follows. First, it showed that the individualized consideration had an effect on the confidence level of leader. Second, it showed that the transforming and transactional leaderships had an effect on the organizational effectiveness, which the hypothesis was adopted. Third, it showed that calculable, intellectual and identical factors had a positive effect on the job satisfaction and organizational commitment, but the organizational commitment of calculable variable was rejected in the confidence level. In general, the effect of transforming and transactional leaderships as public enterprise CEOs' leadership characteristics on the organizational effectiveness by mutual confidence level of calculable, intellectual and identical standards showed that the organizational commitment was rejected in the base of identification. And other factors were adopted as a positive mechanism, which they were a mechanism suitable to public enterprise CEOs' leadership characteristics.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1105-1111
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• 2005

In order to investigate the meanings and bases of transforming theories of diseases(病傳論), several relevant theories were collected from medical books since . The outside-to-inside changing process(pyoree jeon) was the fundamental pattern in febrile diseases regardless of slang han and wen bing after Han dynasty. But in case of numb disease the three exogenous pathogenic factors of wind, cold and dampness got into each viscera through five tissues like skin, vessesl, flesh, tendons, bones respectively. It was called corresponding changing process(sanghap jeon) here. The pathogenic changing process(byungsa jeon) had complicated details to explain pathologic processes, but could give useful informations on evaluating relative strength of pathogens and tendency ahead. The changing process of diseases of internal injuries were explained in case of emotional distress which is outbroken abruptly didn't follow regular order of viscera-emotion relations. So it was named random changing process(bulcha jeon) after 's usage. And marasmus and asthenic disease followed top-to-down or down-to-top changing process(sangha jeon) based on . There are many types of changing process of diseases between viscera and viscera or viscera and bowels like generation changing process, restriction changing process, changing process between couple, changing process of mutual transmission based on attributions of each element by the theory of 5 phases(五行論). And changing process to a neighbor doesn't have any special relations within two organs but has anatomical contiguity and physiological continuity between them. The transforming theories of diseases bring forth useful understanding on comings and goings of pathogenic factors and tendency and prognosis of disease, so they are needed to be taught in the course of pathology class.

• Convergence Security Journal
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• v.12 no.2
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• pp.23-32
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• 2012

This study of transformational leadership impact on followership, job satisfaction and organizational commitment to investigate the relationship. Private security guards and managers that works private security firms located in Seoul, Gyeonggi-do, and Incheon in 2011 were set as a population and purposive sampling method to extract the sample by 880 people, but final samples of 790 people were drawn from the results. This study SPSSWIN 16.0 using factor analysis, reliability analysis, Pearson's Correlation Analysis, multiple regression analysis, path analysis was carried out. In addition, this study is to investigate the influence of transforming leadership with followership, job satisfaction and organizational commitment. The results are as follows. First, transforming leadership affects followership. Second, transforming leadership affects job satisfaction and organizational commitment. Third, followership affects partially In other words, behavioral tendencies increases job satisfaction and organizational commitment. Thinking tendencies is also job satisfaction and organizational commitment does not affect.

• Cartoon and Animation Studies
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• s.39
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• pp.85-109
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• 2015

have been transformed to many products and game of various platform as successful animation be making for 26 seasons. However past studies were concentrated on characteristic of as an TV animation series, almost never focused on transforming strategy. is showing distinguished strategy for how long living TV animation series can be made to user participating contents constantly. This paper analyzed a characteristic transforming strategy to game of as focusing on that EA company was made for mobile platform and it has been popular for a long time comparatively. So first we analyzed characteristic narrative of animation, and then searched how it is appeared to space managing simulation game . It will be helpful study how to build narrative strategy when TV animation series will be transformed to game as analyzing gamification process of .

• Journal of Korean Library and Information Science Society
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• v.47 no.4
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• pp.339-358
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• 2016

This study is to demonstrate that public libraries help community to become an active 'Community of Practice(CoP)'. In order to prove this hypothesis, 'Libraries Transforming Communities(LTC)' initiative of American Library Association is analyzed. Public libraries participating in LTC program solved problems of communities through making CoPs by librarians as a catalyst. People's recognition about public libraries is altered by the problem-solving practice. The followings are 4-level transforming relationship model of public library and community: Leadership Development for Librarian CoPs; Librarian CoPs for improving library services; Community CoPs with library for solving problems; Community as CoP.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.6
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• pp.388-388
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• 2000

A design method for a multisection impedance transforming branch-line hybrid using a genetic algorithm suitable for MMIC applications is proposed. In contrast to the previous design methods, an asymmetric structure is introduced to optimize the hybrid. Optimization is performed within the impedance range to achieve the realizable hybrids with a microstrip line in a desired frequency range. This design method is applicable to the hybrid which has the arbitrary power division ratio, impedance transforming ratio, isolation, directivity and bandwidth. The hybrid designed by the proposed method has 3∼10％ more bandwidth than the previous results.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.6
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• pp.28-35
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• 2000

A design method for a multisection impedance transforming branch-line hybrid using a genetic algorithm suitable for MMIC applications is proposed. In contrast to the previous design methods, an asymmetric structure is introduced to optimize the hybrid. Optimization is performed within the impedance range to achieve the realizable hybrids with a microstrip line in a desired frequency range. This design method is applicable to the hybrid which has the arbitrary power division ratio, impedance transforming ratio, isolation, directivity and bandwidth. The hybrid designed by the proposed method has 3∼10％ more bandwidth than the previous results.

• Environmental Mutagens and Carcinogens
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• v.19 no.1
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• pp.20-27
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• 1999

In order to know the inhibitory effect of magnesium carbonate(MgCO3) on cytotoxicity, DNA damage, mutagenicity, and cell transforming ability of nickel subsulfide, the inhibition of cell proliferation, DNA-protein crosslinks formation (DPC), HGPRT point mutation, and cell transformation were evaluated. Nickel subsulfide(Ni3S2) and magnesium carbonate as insoluble compounds were used for this study. BALB/3T3 cell, CHO-K1 cell, and C3H10T1/2 cell were used in this experiment. Exposure concentration of nickel subsulfide was 1 $\mu\textrm{g}$/ml. The concentrations of magnesium carbonate in this study were 0.6 $\mu\textrm{g}$/ml, 1.2 $\mu\textrm{g}$/ml, 2.4 $\mu\textrm{g}$/ml and the molar ratio of magnesium to nickel when exposed simultanously were 0.5, 1.0 and 2.0 respectively. The results were as follows; 1. Magnesium carbonate reduced the inhibitory effect of nickel subsulfide on cell proliferation. 2. Magnesium carbonate also reduced the effect of nickel subsulfide on DNA-protein crosslinks formation. 3. HGPRT point mutagenicity of nickel subsulfide was reduced when magnesium carbonate treated simultaneously. 4. Magnesium carbonate reduced cell transforming ability of nickel subsulfide. Conclusively, nickel subsulfide showed cytotoxicity, cell transforming ability, and mutagenicity strongly and magnesium carbonate may have protective roles in these nickel effects.

• Korean Journal of Veterinary Service
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• v.21 no.3
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• pp.267-275
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• 1998

돼지 수정란의 체외생산은 난자의 체외성숙과 체외수정에 관한 기술의 부족으로 아직까지 만족스럽지 못한 수준이다. 특히 돼지 수정란의 체외생산에는 복잡한 세포질의 성숙과정과 높은 다정자침입율 및 전핵형성의 억제등의 문제점이 있다. 본 연구에서는 돼지 난자의 체외성숙 체계를 개선하기 위하여 transforming growth factor$\beta$(TGF$\beta$)의 첨가가 난자 및 난구세포에 미치는 효과에 대하여 검토하였다. 체외성숙용 배지에 TGF$\beta$를 1~10ng/$m\ell$의 농도로 첨가하여 미성숙 난자를 배양한 결과 성숙율이 높아졌다. TGF$\beta$의 효과는 난구세포가 제거된 난자의 성숙에도 효과적이었다. TGF$\beta$(를 첨가하지 않은 배양액 내에서는 배양 24시간 까지 metaphase-II로 성숙된 난자가 관찰되지 않았으나 TGF$\beta$를 첨가한 배양액 내에서는 관찰되었다. 한편, 난구세포가 부착된 난자의 성숙배양시 TGF$\beta$의 첨가시기에 따른 차이는 없었으나, 난구세포를 제거한 난자의 경우에는 성숙배양 전반기(59%) 또는 후반기(57%) 24시간 동안에만 TGF$\beta$를 첨가하는 것이 48시간 동안 계속하여 첨가(27%)하는 경우 또는 비첨가(38%)에 비하여 유의적으로 높은 성숙율을 나타냈다(p<0.05). 이와 같은 결과는 난구 세포가 돼지 난자의 체외성숙에 필수적이지만 TGF$\beta$는 난구세포가 제거된 난자의 체외성숙에 어 느정도 유익한 효과를 발휘하는 것으로 추측된다.