• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.6
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• pp.807-814
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• 2016

Fucoidan, one of the dominant sulfated polysaccharides extracted from brown seaweed, possesses a wide range of biological activities. Transforming growth $factor-{\beta}$ ($TGF-{\beta}$) plays a pivotal role in the pathogenesis of pulmonary fibrosis, by stimulating the synthesis of profibrotic factors. In this study, we investigated the in vitro effects of fucoidan on collagen synthesis, ${\alpha}-smooth$ muscle actin (${\alpha}-SMA$) expression, and interleukin (IL)-6 production in $TGF-{\beta}$-stimulated human pulmonary fibroblasts. The expression of type I collagen and ${\alpha}-SMA$ was detected by Western blot, and the production of IL-6 by enzyme-linked immunosorbent assay. $TGF-{\beta}1$ treatment of pulmonary fibroblasts enhanced the expression of ${\alpha}-SMA$, type I collagen, and IL-6 whereas these effects were inhibited in cells pretreated with fucoidan. The activation of Smad2/3, p38 mitogen-activated protein kinases (MAPKs), and Akt was also inhibited in fucoidan-pretreated, $TGF-{\beta}1-stimulated$ human pulmonary fibroblasts. These data demonstrate the anti-fibrotic potential of fucoidan in $TGF-{\beta}-induced$ human pulmonary fibroblasts, via the inhibition of Smad2/3, p38 MAPKs, and Akt phosphorylation. Our results suggest the therapeutic potential of fucoidan in the prevention or treatment of pulmonary fibrosis.

• Korean Journal of Construction Engineering and Management
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• v.13 no.5
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• pp.144-152
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• 2012

The reason why most scheduling softwares currently using at construction projects adopt PDM (Precedence Diagramming Method) is that it can express an overlapping relationship between activities. However, the overlapping relationships in PDM are represented only by combinations of four overlapping types that connect the start and finish points of two activities, therefore, PDM cannot express efficiently the relationships between any middle points of two activities if they should be represented. This research proposes the method of transforming the four overlapping types of PDM to the overlapping relationships of BDM(Beeline Diagramming Method), new networking technique, that can connect the inter-relationships at any middle points of two activities as well as express multiple overlapping relationships. The proposed method will help not only to improve an efficiency of scheduling but also to upgrade the scheduling technique because BDM technique has a lot of unique advantages in scheduling.

• The Journal of the Acoustical Society of Korea
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• v.16 no.1
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• pp.39-46
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• 1997

In this paper, the PA(PhotoAcoustic) signals excited in metals by Xef, KrF excimer laser pulse were detected by a PZT transducer, and its transforming machanism and directivity patterns were analysed. The laser energy density in irradiation spot divides the PA trasnsorming machanism to be classified into thermoelastic and plasma regime, and the transforming machanisms in two regimes are different from each other. Based on theoretical model, it is predicted that shear wave is greater than longitudinal in the thermoelastic regime and longitudinal is greater than shear wave by reaction force in plasma regime. These predictions were verified through experiments by using of the XeF excimer pulsed laser of 480nm center-wavelength and the KrF excimer pulsed laser of 248nm. Also, for its directivity pattern, an arrival angle of the maximum longitudinal energy was around $60^{\circ}$ and maximum shear energy was around $30^{\circ}$ in the thermoelastic regime, and an arrival angle of maximum longitudinal energy was shown on nomal to the surface and maximum shear energy was represented in about $30^{\circ}$ in plasma regime.

• Macromolecular Research
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• v.13 no.4
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• pp.285-292
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• 2005

We developed alginate beads loaded with transforming growth $factor-{\beta}_{1}(TGF-{\beta}_{1})$ to examine the possible application of the scaffold and cytokine carrier in tissue engineering. In this study, bone marrow stromal cells (BMSCs) and $TGF{\beta}_{1}$ were uniformly encapsulated in the alginate beads and then cultured in vitro. The cell morphology and shape of the alginate beads were observed using inverted microscope, scanning electron microscope (SEM), histological staining and RT-PCR to confirm chondrogenic differentiation. The amount of the $TGF{\beta}_{1}$ released from the $TGF-{\beta}_{1}$ loaded alginate beads was analyzed for 28 days in vitro in a phosphate buffered saline (pH 7.4) at $37^{\circ}C$. We observed the release profile of $TGF-{\beta}_{1}$ from $TGF-{\beta}_{1}$ loaded alginate beads with a sustained release pattern for 35 days. Microscopic observation showed the open cell pore structure and abundant cells with a round morphology in the alginate beads. In addition, histology and RT-PCR results revealed the evidence of chondrogenic differentiation in the beads. In conclusion, these results confirmed that $TGF-{\beta}_{1}$ loaded alginate beads provide excellent conditions for chondrogenic differentiation.

• Journal of the Korea Society of Computer and Information
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• v.16 no.3
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• pp.53-63
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• 2011

Many techniques have been proposed to store and query XML data efficiently. One way achieving this goal is using relational database by transforming XML data into relational format. It is important to transform schema to preserve the content, the structure and the constraints of the semantics information of the XML document. Especially, key constraints are an important part of database theory. Therefore, the proposal technique has considered the semantics of XML as expressed by primary keys and foreign keys. And, the proposal technique can preserve not only XML data constraints but also the content and the structure and the semantics of XML data thru transformation process. Transforming information is the content and the structure of the document(the parent-child relationship), the functional dependencies, semantics of the document as captured by XML key and keyref constraints. Because of XML schema transformation ensures that preserving semantic constraints, the advantages of these transformation techniques do not need to use the stored procedure or trigger which these data ensures data integrity in the relational database. In this paper, there is not chosen the ID/IDREF key which supported in DTD, the inheritance relationship, the implicit referential integrity.

• Molecules and Cells
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• v.40 no.3
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• pp.211-221
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• 2017

Transforming growth factor ${\beta}1$ $(TGF{\beta}1)/Smad4$ signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differentiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through $TGF{\beta}1/Smad4$ signaling. Here, we present that $TGF{\beta}1$ elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL. Additionally, we found that silencing Smad4 attenuated the upregulation of miR-155 induced by $TGF{\beta}1$. The results of luciferase reporter experiments and ChIP assays demonstrated that $TGF{\beta}1$ promoted the binding of Smad4 to the miR-155 promoter at a site located in 454 bp from the transcription start site in vivo, further verifying that miR-155 is a transcriptional target of the $TGF{\beta}1/Smad4$ pathway. Subsequently, TRAP staining and qRT-PCR analysis revealed that silencing Smad4 impaired the $TGF{\beta}1$-mediated inhibition on osteoclast differentiation. Finally, we found that miR-155 may target SOCS1 and MITF to suppress osteoclast differentiation. Taken together, we provide the first evidence that $TGF{\beta}1/Smad4$ signaling affects osteoclast differentiation by regulation of miR-155 expression and the use of miR-155 as a potential therapeutic target for osteoclast-related diseases shows great promise.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.3
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• pp.475-485
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• 2017

This paper develops a conversion function and method transforming from daily peak load curve used $LOLE_D$ [days/year] to hourly load curve used $LOLE_H$[hours/year]and describes relationship between $LOLE_D$ [days/year] and $LOLE_H$ [hours/year]. The indices can not only be transformed just arithmetically but also have different characteristics physically because of using their different load curves. The conversion function is formulated as variables of capacity and forced outage rate of generator, hourly load daily load factor and daily peak load yearly load factor, etc. Therefore, the conversion function (${\gamma}={\varphi}$(.)) can not be simple. In this study, therefore, the function is formulated as linear times of separated two functions. One is an exponential formed conversion function of daily load factor. Another is formulated with an exponential typed conversion function of daily peak load yearly load factor. Futhermore, this paper presents algorithm and flow chart for transforming from $LOLE_D$[days/year] to $LOLE_H$[hours/year]. The proposed conversion function is applied to sample system and actual KPS(Korea Power System) in 2015. The exponent coefficients of the conversion functions are assessed using proposed method. Finally, assessment errors using conversion function for case studies of sample system and actual system are evaluated to certify the firstly proposed method.

• Journal of KIISE:Software and Applications
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• v.31 no.7
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• pp.883-900
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• 2004

As object-oriented development technology that increases extensibility and reusability has been widely spread, it can shorten development period and enhance quality by reusing verified object-oriented artifacts. Thus we can construct high quality component-based system at short time transforming component-based model using verified object-oriented artifacts. In this paper, we propose techniques to transform available object-oriented design model into component-based model using formal specification techniques in order to increase accuracy of transformation. First, formal specification language for component is defined for formal specification of component-based design. And, techniques for formal specification of object-oriented design using Object-Z, a formal specification language, is proposed in structural, functional, and dynamic aspects. Next, we present techniques for transforming formal specification of object-oriented design into formal specification of component-based design. Through a case study we apply the proposed transformation techniques and show the transformation process of object-oriented formal specification into component-based formal specification.

• The Transactions of the Korea Information Processing Society
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• v.4 no.7
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• pp.1665-1680
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• 1997

The Entity-Relationship (E-R) model is widely used not only to increase understanding between user and designer, but also to model the relationship of real world data appropriately when designing database system in many application areas. It should be then transformed into an Object-Oriented database model which gives good merits to represent and manipulate data efficiently. Therefore, a method of transforming an E-R model into an Object-Oriented database model should be studied, but without losing any semantics of concept for the E-R model. This paper not only deals with transformation rules taking as input the elements of E-R model and delivering the elements of an Object-Oriented database model, but also improves the concept of generalization and aggregation inheritance. The paper also presents a method of transformation of relationship depending on these rules. The proposed method that obtains Object-Oriented database schema from an E-R model with preserving the properties of the E-R model is shown with examples. The method presented is able to be used to the logical database design.

• Journal of the Society of Disaster Information
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• v.10 no.2
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• pp.254-262
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• 2014

KISStool is developed and established by KIGAM, in order to link and share the earthquake data easily with institutes related, this study includes the development of a KISStool program format and protocol supporting Kit. The Kit has functions such as transforming and sending a seismic data. The functions of the kit are implemented in modules, which are running on the realtime basis. The firmware is embedded into the Kit, which transforms the seismic data into the formats, miniSEED and QSCD20. These data are sent to KISStool program. We have confirmed the results of the data transforming, transmissions and storage functions are conducted normally, the speed of transmission shows same level when comparing with a standard system. We expect that the firmware developed in this study will have high usability not only for linking with KISStool program through being embedded into incompatible digitizers, but also for improving performance of older ones.