• 제어로봇시스템학회:학술대회논문집
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• 1993.10a
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• pp.1058-1062
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• 1993

A fuzzy reasoning method is proposed for the implementation of control systems based on non-fuzzy microprocessors. The essence of the proposed method is to search the local active miles instead of the global rule base. Thus the reasoning is conveniently performed on a master cell as a fuzzy accelerating kernel, which is transformed from an active fuzzy cell. The interpolative reasoning is simplified via adopting the algebraic product of fulfillment for the conditional connective AND and the weighted average for the rule sentence connective ALSO.

• 제어로봇시스템학회:학술대회논문집
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• 1988.10a
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• pp.374-379
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• 1988

We propose a framework for modelling and operation management of robotic assembly cells via knowledge base. In the framework, each component of the cell is considered as a state variable, the relations among the state variables are stored in state transition maps(STMs) and then transformed into the form of knowledge. The assembly job tree(AJT) which includes the precedence relations and the constraints for assembly tasks is also described. Finally, an algorithm is presented to manage the cell operation.

• Journal of Welding and Joining
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• v.13 no.3
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• pp.46-54
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• 1995

The structural changes of Al-Cr alloys due to the difference in the growth rates were investigated in the study using the water cooled copper chill apparatus, the levitation apparatus, and the melt spinner. Growth rate was evaluated by means of thermal analysis could measured the cooling rate up to 10$^{5}$ K/sec. The transformation from the cell structure to the massive transformed structure was obtained the Al-3.43wt%Cr alloy in the melt spinner method.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.156-156
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• 2001

We have previously shown that H-ras, but N-ras, induces an invasiveness and cell motility in human breast epithelial cells (MCFl0A), while both H-ras and N-ras induce transformed phenotype. It has been recently shown that phosphatidylinositol 3-kinase (PI3K) plays an important role on cell migration. In the present study, we wished to investigate the functional role of PI3K in H-ras-induced invasive phenotype in MCF10A cells.(omitted)

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.134-134
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• 2004

• Macromolecular Research
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• v.10 no.4
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• pp.181-186
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• 2002

The displacement of carbon black to polypyrrole as a catalyst supporter in the fuel electrode of a direct methanol fuel cell was investigated. Polypyrrole was obtained as a black powder by the chemical polymerization of pyrrole with three different oxidants. The synthesized polypyrroles were pasted on carbon paper and transformed to the fuel electrodes with electrochemically deposited platinum. The prepared fuel electrode was assembled and mounted in a unit cell using a membrane and cathodic electrode film. In comparison with the carbon black fuel electrode, the performance of the unit cell was analyzed in relation to the state of the catalyst, the type of oxidant, and the morphology of the polypyrrole powder.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.2
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• pp.142-148
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• 2005

This paper proposes a cell age model for Penicillium chrysogenum fed-batch cultivation to supply a qualitative insight into morphology-associated dynamics. The average ages of the segregated cell populations, such as growing cells, non-growing cells and intact productive cells, were estimated by this model. A combined model was obtained by incorporating the aver-age ages of the cell sub-populations into a known but modified segregated kinetic model from literature. For simulations, no additional effort was needed for parameter identification since the cell age model has no internal parameters. Validation of the combined model was per-formed by 20 charges of industrial-scale penicillin cultivation. Meanwhile, only two charge-dependent parameters were required in the combined model among approximately 20 parameters in total. The model is thus easily transformed into an adaptive model for a further application in on-line state variables prediction and optimal scheduling.

• Journal of Plant Biology
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• v.36 no.3
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• pp.285-292
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• 1993

Cellular compatibility in the self-parasitism of Cuscuta australis R. Brown was studied at the ultrastructural level. The front cells of the haustorium penetrated the host stems independently grew within the host tissues and transformed into elongate, filamentous hyphae. Each hyphal cells contained a large nucleus and dense cytoplasm with abundant cell organelles. Multilamellar structures were contained in the cytoplasm and cell walls of the penetrating hyphal cells. When the hyphal cells did not yet invade the host cells, the middle lamella and the fused cellulosic cell walls of the two partners at the host-parasite interface were preserved well. As the invasion of the parasitic hyphal cells progressed, however, the middle lamella was not found at the interface and the host cell walls and plasma membranes were partially broken down. A hyphal cell penetrated deeply into the host cell had a more darkly stained cytoplasm with numerous of cell organelles. In the host cells attacked by the hyphal cells the limiting membranes of plastids were broken down and several vesicles were arrayed near the cell walls. No plasmodesmatal connections between the host and parasite cell walls were found; however, half-plasmodesmata were observed frequently on the side of the hyphal cell walls. These results suggested that the compatibility response in the self-parasitism of Cuscuta was expressed by cell walls, not by plasmodesmata, between the host and the parasite cells.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.59-63
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• 1999

A cDNA encoding rice chloroplast small HSP, Oshsp21, was introduced into Escherichia coli using the pET expression vector to analyze the possible function of Oshsp21 under heat stress. We compared the viability of E. coli ${\lambda}BL21$ (DE3) cells transformed with recombinant plasmid containing Oshsp21 with the control E. coli cells transformed with pET28a vector under heat stress after IPTG induction. Upon heat treatment at $50^{\circ}C$, those cells that expressed Oshsp21 showed improved viability compared with control cells. When the cell lysates from E. coli transformants were heated at $55^{\circ}C$, the amounts of proteins denatured in the control and pEhsp21-transformed cells were about 60% and 35% of total cell proteins, respectively. These results indicate that rice chloroplast small HSP function as a molecular chaperone in cells.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.3
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• pp.225-229
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• 2005

To investigate impediments to plasmid transformation in Brevibacterium flavum BF4 and B. lactofermentum BL1, cell surface barriers were determined by measuring growth inhibition whilst enzymatic barriers were determined by comparing DNA methylation properties. B. lactofermentum was more sensitive to growth inhibition by glycine than B. flavum. Release of cellular proteins during sonication was more rapid for B. lactofermentum than for B. flavum. Plasmid DNA (pCSL 17) isolated from B. flavum transformed recipient $McrBC^+$ strains of Escherichia coli with lower efficiency than $McrBC^-$. McrBC digestion of this DNA confirmed that B. flavum contain methylated cytidines in the target sequence of McrBc sequences but B. lactofermentum contained a different methylation pattern. DNA derived from the B. lactofermentum transformed recipient $EcoKR^+$ strains of E. coli with lower efficiency than $EcoKR^-$, indicating the presence of methylated adenosines in the target sequence of EcoK sequences. The present data describe the differences in the physical and enzymatic barriers between two species of corynebacteria and also provide some insight into the successful foreign gene expression in corynebacteria.