• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.283-288
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• 1998

The bialaphos is a potent inhibitor of glutamine synthease in higher plants and is used as a non-selective herbicide. We have used the bialaphos resistant gene(Bar) encoding for an acetyltransferase isolated from Streptomyces hygroscopicus SF1293. Callus derived from mature seeds of rice(Oryza sativa L. cv. Dong Jin) were co-cultivated with Agrobacterium tumefaciens EHA101 carring a plasmid pGPTV-HB containing genes for hygromycin resistance (HygR) and Bar. Transgenic plants showing in vitro resistance to 50 mg/L hygromycin and 10 mg/L bialaphos were obtained by using a two-step selection/regeneration procedure. Transformation efficiency of rice was about 30% which was as high as reported in other dicotyledons. Progenies ($\textrm{T}_{1}$ generation) derived from primary transformant of 17 lines were segregated with a 3 resistant : 1 sensitive ratio in medium containing hygromycin and bialaphos. Stable integration of Bar gene into chromosomal DNA was proven by Southern blot analysis of genomic DNA isolated from $\textrm{T}_{2}$ progenies. Transgenic plants ($\textrm{T}_{3}$) grown in the field were resistant to bialaphos (Basta) at a dosage lethal to wild type plants.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.1-7
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• 2004

This study is kinematics for the manipulator development of cucumber harvesting. A theory value was verified by repeated error measurement after the forward kinematics or inverse kinematics analysis of manipulator. Manipulator is consisted of one perpendicular link and two revolution link. The transformation of manipulator can be valued by kinematics using Denavit-Hartenberg parameter. The value of inverse kinematics which is solved by three angles faction shows two types. Repeated errors refered maximum 2.60 mm, 2.05mm and 1.55 mm according to X, Y, Z axis. In this study, the actual coordinates of maximum point and minimum point were agreement in the forward kinematics or inverse kinematics. The results of repeated error measurement were reflect to be smaller compared to a diameter of cucumber. measurement errors were determined by experimented errors during the test. For reducing errors of manipulator and improving work efficiency, the number of link should be reduced and breeding and cultural environment should be considered to reduce the weight and use the hard stuff. The velocity of motor for working should be considered, too.

• Journal of Aerospace System Engineering
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• v.15 no.1
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• pp.80-85
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• 2021

In this study, for conducting a static load test of an external fuel tank used for an aircraft, the flight load acting on the external fuel tank was converted to the test load and the suitability of the converted test loads was confirmed. In order to calculate the test load from the flight load, the external fuel tank was divided into several sections. Shear load, moment by unit shear load, and unit moment were calculated for each section. Test loads for each section were then calculated by computing the shear load, the moment of each section, and flight load condition. In actual static load tests, it might not be possible to impose the test load in the calculated position due to physical constraints. Therefore, after determining positions in which the load could be imposed in the actual test, the test load calculated for each section was redistributed to selected positions. Finally, a test load plan was established by applying a whiffle tree to enhance the efficiency of the test performance while making it easier to operate the actuator. The reliability of the test load plan was verified by comparing it with flight load conditions.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.40 no.5
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• pp.403-412
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• 2022

As the 4th industrial revolution is in full swing and next-generation ICT(Information & Communications Technology) convergence technology is being developed, various smart construction technologies are being rapidly introduced in the construction field to respond to technological changes. In particular, since the earth-volume calculation process for site design accounts for a large part of the design cost at the construction site, related researches are being actively conducted to improve the efficiency of the process and accurately calculate the earth-volume. The purpose of this study is to present a method for quickly constructing the topography of a construction site in 3D and efficiently calculating earth-volume using the results. For this purpose, the construction site was constructed as a 3D realistic model using large-scale aerial photos obtained from UAV(Unmanned Aerial Vehicle). At this time, since the constructed 3D realistic model has a surface model structure in which volume calculation is impossible, the structure was converted into a 3D solid model to enable volume calculation. And we devised a methodology to calculate earth-volume based on CAD(Computer-Aided Design and Drafting) using the converted solid model. Automatically calculating earth-volume from the solid model by applying the method. As a result, It was possible to confirm a relative deviation of 1.52% from the calculated earth-volume from the existing survey results. In addition, as a result of comparative analysis of the process time required for each method, it was confirmed that the time required is reduced of 60%. The technique presented in this study is expected to be utilized as a technology for smart construction management, such as periodic site monitoring throughout the entire construction process, as well as cost reduction for earth-volume calculation.

• Journal of the Korean Institute of Gas
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• v.26 no.4
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• pp.41-57
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• 2022

The global demand for carbon neutrality in response to climate change is in a situation where it is necessary to prepare countermeasures for carbon trade barriers for some countries, including Korea, which is classified as an export-led economic structure and greenhouse gas exporter. Therefore, digital transformation, which is one of the predictable ways for the carbon-neutral transition model to be applied, should be introduced early. By applying digital technology to industrial gas manufacturing facilities used in one of the major industries, high-tech manufacturing industry, and hydrogen gas facilities, which are emerging as eco-friendly energy, abnormal detection, and diagnosis services are provided with cloud-based predictive diagnosis monitoring technology including operating knowledge. Here are the trends. Small and medium-sized companies that are in the blind spot of carbon-neutral implementation by confirming the direction of abnormal diagnosis predictive monitoring through optimization, augmented reality technology, IoT and AI knowledge inference, etc., rather than simply monitoring real-time facility status It can be seen that it is possible to disseminate technologies such as consensus knowledge in the engineering domain and predictive diagnostic monitoring that match the economic feasibility and efficiency of the technology. It is hoped that it will be used as a way to seek countermeasures against carbon emission trade barriers based on the highest level of ICT technology.

• Journal of the Korean Society for information Management
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• v.39 no.3
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• pp.165-193
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• 2022

It can be said that digital technology is at the center of the change in the modern work environment. In particular, in general public institutions that prove their work with records produced by business management systems and document production systems, the record management system is also the work environment itself. Gimpo City applied for the 2021 public cloud leading project of the National Information Society Agency (NIA) to proactively respond to the 4th industrial revolution technology era and implemented a public cloud-based AI-OCR technology enhancement project with 330 million won in support of 330 million won. Through this, it was converted into data beyond the limitations of non-electronic records limited to search and image viewing that depend on standardized index values. In addition, a 98% recognition rate was realized by applying a new technology called AI-OCR. Since digital technology has been used to improve work efficiency, productivity, development cost, and record management service levels of internal and external users, we would like to share the direction of enhancing expertise in the record management and implementation of work environment innovation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.36 no.3
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• pp.261-265
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• 2023

In this paper, we analyzed the transformation of the power following by the angle of incidence of the solar, the angle of photovoltaic module and artificial solar changed from 30° to 90° and synchronously changed the distance from 0.1 m to 0.5 m. Setting the distance between the artificial solar and the luminometer from 0.1 m to 0.5 m and set the angles to 90°, 60°, 45°, and 30°, the angle was 90° and when the distance was 0.1 m, the maximum Illuminance was 19,580 lux, the light could be obtained more. If the angle of incidence between the Artificial solar and the photovoltaic module was 90° and the variable resistance was 1,000 Ω at a distance of 0.4 m, the maximum power reached 0.82 W. Provided that the angle of incidence between the artificial solar and the photovoltaic module was 90° and the distance was 0.2 m since the variable resistance had the maximum power of 500 Ω, the maximum power was 0.78 W. At 1,000 Ω, the maximum power is 0.80 W so the maximum power at the variable resistance 1,000 Ω could obtain higher power than the variable resistance 500 Ω. The variable resistance was 1,000 Ω and the angle of incidence between the Artificial solar and the photovoltaic module was 90° at a distance of 0.4 m, and the maximum power reached 0.82 W. The angle was 60° at 0.3 m and 0.4 m the maximum power reached 0.10 W. The angle was 45° at 0.2 m maximum power reached 0.020 W, the angle was 30° at 0.4 m, and the maximum power reached 0.004 W. In four results about maximum power depending on the angle of incidence between the artificial solar and the photovoltaic module, the luminous efficiency and maximum power can be got the best at an angle of 90°.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.37 no.1
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• pp.36-42
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• 2024

Roll-to-roll processing holds an integral position within the manufacturing landscape, and its significance reverberates across numerous industries. This versatile technology platform encompasses a diverse array of process methods and accommodates a wide spectrum of material categories, making it a cornerstone of modern production. Within this expansive domain, two commonly employed coating techniques, namely the slot die and gravure coating methods, have earned their prominence for their precision and efficiency in delivering flawless coatings. Additionally, the realm of drying processes relies heavily on thermal drying, infrared (IR) drying, and ultraviolet (UV) drying methods to expedite the transformation of materials from their liquid or semi-liquid states to solid, ready-to-use products. The undeniable importance of roll-to-roll processing lies in its ability to streamline manufacturing processes, reduce costs, and enhance product quality. This article embarks on a comprehensive journey to fathom the depth of this importance by delving into the intricacies of these common roll-to-roll process methods. Through rigorous research and meticulous data collection, we aim to shed light on the pivotal role these techniques play in shaping various industries and advancing the world of manufacturing. By understanding their significance, we can harness the full potential of roll-to-roll processing and pave the way for innovation and excellence in production.

• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.527-539
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• 2023

Pepper (Capsicum annuum L.) is an important vegetable and spice crop that has been cultivated worldwide. Pepper fruits have unique taste and aroma, providing a variety of antioxidants and compounds important for human health, which makes a high economic value. In addition, there is a high demand for new pepper varieties, according to consumer's preference. However, pepper is a recalcitrant plant for in vitro tissue and organ differentiation and plant regeneration, which makes it difficult to develop demanded varieties using newly developed technologies such as genetic engineering and gene editing. In this study, tissue culture and regeneration conditions were investigated using seven pepper varieties that were obtained from the core-collection of Seoul National University. We observed callus and bud induction and shoot formation using several media composition composed of different cytokinins and auxin concentrations. As a result, it was found that there were differences in callus induction and shoot formation of each variety depending on the hormone composition, and the highest regeneration was shown when the medium containing Zeatin Riboside and the petioles of seedlings were used. In particular, out of seven pepper varieties, CMV980 exhibited a higher regeneration efficiency (approximately 48%) than other varieties, followed by Yuwolcho. Therefore, this study provides CMV980 and Yuwolcho as good candidates that can be used for pepper transformation, which might contribute to the development of various varieties through gene editing technology in the future.

• Journal of the Korean Regional Science Association
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• v.40 no.1
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• pp.37-51
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• 2024

To respond to local extinction, South Korea, under the leadership of the Ministry of the Interior and Safety, identified depopulated areas in 2021 and launched the Local Extinction Response Fund in 2022. However, due to its early stage of implementation, analyzing the characteristics and changes of policy response to local extinction at the central government level remains a challenge. In contrast, Japan, facing similar issues of local extinction as South Korea, has established a robust central government-led response system based on the Regional Revitalization Act and the Comprehensive Strategy and Basic Policy on Community-Population-Job Creation. Hence, this study examines Japan's policy responses to local extinction by analyzing the first and second periods of the Comprehensive Strategy and Basic Policy on Community-Population-Job Creation. For the analysis, topic modeling was employed to enhance text analysis efficiency and accuracy, complemented by expert interviews for validation. The results revealed that the first-period strategy's topics encompassed economy and society, start-up, local government, living condition, service, and industry. Meanwhile, the second-period strategy's topics included resource, the New Normal, woman, digital transformation, industry, region, public-private partnership, and population. The analysis highlights that the policy target, policy direction, and environmental change significantly influenced these policy shifts.