• KSBB Journal
• /
• v.10 no.1
• /
• pp.23-29
• /
• 1995

Calli were induced from leaf base region of germinated rice(Oryza sativa L. cv. Nakdong) with high frequency of up to 65% on LS medium supplemented with $2.5mg/{\ell}2$, 4-D in the dark at $27^{\circ}C$. Embryogenic calli of pale yellow, globular type were selected and used for the initiation of cell suspension cultures in AA2 liquid medium with $2mg/\ell$ 2,4-D, 0.2mg/$\ell$ kinetin arid $0.1mg/\ell$ GA3. Protoplasts were isolated from the embryogenic cell suspensions after 4 months of culture and then were electroporated with 400V/cm for 1 msec. Electroporated protoplasts divided with plating efficiency of 1.1% on PCM liquid medium supplemented with $2.5mg/\ell$ 2, 4-D, $0.1mg/\ell$ kinetin and 10mM proline. The protoplasts-derived microcalli were cultured on $0.2{\mu}m$ membrane fitter placed onto LS2.5 solid medium containing fine suspension cells as a feeder cells, for 2 weeks in the dark at $27^{\circ}C$. After an additional 2 weeks of culture under fluorescent light of $30{\pm}/3{\mu}E$.m^{-2}S^{-1}, yellow calli of 2mm diameter were transferred to regeneration medium. Shoots were produced from the green spot of protoplasts-derived calli and plants were regenerated form protoplast-derived green calli with frequencies of 11∼33%.

• International Journal of Highway Engineering
• /
• v.12 no.3
• /
• pp.93-101
• /
• 2010

As the number of indispensable needs of clean energy increases due to the green new deal revolution, the possibility of heat energy harvesting from the surrounding infrastructures such as a railroad or highway was verified. In order to find more efficient usage of a heat source, the possibility of transforming heat into electricity were confirmed using Bi-Te type thermoelectric element, and electrical quality were tested with experiments of different heat source and environmental change in the surrounding infrastructures. After careful experiments, the possibility of collecting thermal energy and findings of the heat temperature change in infrastructrue are verified with a result of obtaining almost 20.82W in 70 celcius($^{\circ}C$) temperature differences and $1m^2$ surface area. Consequently, the ratio of heat temperatiure change and transforming surface area is the most crucial factor in the harvesting heat energy, and reducing thermal loss and improving thermal convection as well as transformation efficiency of thermoelectric element is required to get more efficient and durable generation.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.3
• /
• pp.52-60
• /
• 2018

In the automobile industry, in order to increase the fuel efficiency and conform to the safety regulations, it is necessary to make the vehicles as light as possible. Therefore, it is crucial to manufacture dual phase steels, complex phases steels, MS steels, TRIP steels, and TWIP from high strength steels with a tensile strength of 700Mpa or more. In order to apply ultra-high tensile strength steel to the body, the welding process is essential. Resistance spot welding, which is advantageous in terms of its cost, is used in more than 80% of cases in body welding. It is generally accepted that ultra-high tensile strength steel has poor weldability, because its alloy element content is increased to improve its strength. In the case of the resistance spot welding of ultra-high tensile steel, it has been reported that the proper welding condition area is reduced and interfacial fracture and partial interfacial fracture occur in the weld zone. Therefore, research into the welding quality judgment that can predict the defect and quality in real time is being actively conducted. In this study, the dynamic resistance of the weld was monitored using the secondary circuit process variables detected during resistance spot welding, and the factors necessary for the determination of the welding quality were extracted from the dynamic resistance pattern. The correlations between the extracted factors and the weld quality were analyzed and a regression analysis was carried out using highly correlated pendulums. Based on this research, a regression model that can be applied to the field was proposed.

• Journal of Broadcast Engineering
• /
• v.12 no.2
• /
• pp.80-92
• /
• 2007

IPTV Services that is representative union service of broadcasting and telecommunication need guarantee of QoS, efficiency of multicasting, and hish bandwidth on the network. Because typical TDM based metro transport network was designed by transporting fixed voice traffic with stable and recovering method, it has a defect of bottleneck and a waste of bandwidth for acceptance of data traffic with burst feature and then all of data are treated equally at the transport network because it cannot classify between advanced high end service and best effort low end service. for completely resolving this kind of problem about increasing burst traffic and QoS issues, firstly we need to new design for transport network. This paper presents transformation method from TDM based metro transport network to packet based transport network and advantage and effectiveness of packet based transport network and also indicates technical factor and characters about method of packet transport system. As a result of research, the Packet Transport System, which is a transmission network for packet delivery, take in not only a specific character of legacy TDM but QoS, Multicast and high bandwidth, then, it is able to keep an effective bandwidth and a stabilized performance of packet transmissions. Additionally, if a fault be occurred on an optical link, the system is able to guarantee a differential QoS by an each service class using an algorithm to make certain of a traffic existence and contain a protective mechanism.

• Journal of Plant Biotechnology
• /
• v.37 no.3
• /
• pp.319-326
• /
• 2010

An interest in the production of seed-oil based fuel and raw materials, which comes from renewable plant sources, has been intrigued by the phenomenon of global warming and shortage of fossil fuels. Rapeseed (Brassica napus) is the most important oilseed crop, which produces seeds with 40% oil. It is desirable to develop genetically modified rapeseed producing oils, which can be easily converted to biodiesel. As an initial step for development of genetically modified rapeseed for the production of biofuels or bio-based materials, Korean rapeseed cultivars, Naehan, Youngsan, Tammi and Halla, were analyzed. Four Korean rapeseed cultivars produce 32 to 40% oil of seed dry weight, which is rich in oleic acid (more than 60 mole%). The cotyledonary petioles of rapeseed cultivar, Halla, were transformed using Agrobacterium tumefaciens strain GV3101, carrying the uidA gene encoding $\beta$-glucuronidase (GUS) as a reporter gene and the phosphinothricin acetyltransferase (PAT) gene as a selectable marker. The stable integration of PAT gene in the genome of transgenic rapeseeds was confirmed by PCR analysis. Expression of uidA gene in various rapeseed organs was determined by fluorometric assay and histochemical staining. Transformation efficiency of a Korean rapeseed Halla cultivar was 10.4%. Genetic inheritance of transgenes was confirmed in $T_2$ generation.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.42 no.3
• /
• pp.134-147
• /
• 2006

A model experiment using circulation water channel was carried out to investigate the dynamic characteristics of bottom trawl net which can be used in sea mount of North Pacific. Hydrodynamic resistance and shape variation according to the flow velocity and angle of hand rope transformation for net were measured, and experimental value was analyzed as the value of full-scale bottom trawl net. The results summarized are as follows; At the $30^{\circ}$ of angle of hand rope to net, hydrodynamic resistance varied from 0.5kgf to 2.68kgf as the flow velocity increased between 0.31m/s and 0.92m/s, and formula of hydrodynamic resistance for the model net was $F_m=3.04\;{\cdot}\;{\upsilon}^{1.53}$. At the fixed angle of hand rope, Net height was low and Net width was high according to the increase of flow velocity, and in addition, vertical opening was low and Net width was high by the increase of angle of hand rope at the fixed flow velocity. At the $30^{\circ}$ of angle of hand rope to net, net opening area was $0.214m^2$ as flow velocity was 0.61m/s, and formula of net opening area for the model net was $S_m=-0.22{\upsilon}+0.35$. At the $30^{\circ}$ of angle of hand rope to net, catch efficiency seemed to be highest as $0.319m^3/s$ of filtering volume at the 0.76m/s(51kt's) of flow velocity. Shape variation of net showed the gradual laminar transform for the variation of flow velocity but there needed some improvements due to the occurrence of shortening at the ahead of wing net.

• Journal of KIISE:Computing Practices and Letters
• /
• v.14 no.2
• /
• pp.130-142
• /
• 2008

Different from other ontology languages, TopicMap is capable of integrating numerous amount of heterogenous information resources using the locational information without any information transformation. Although many conventional editors have been developed for topic maps, they are standalone-type only for writing XTM documents. As a result, these tools request too much time for handling large-scale data and provoke practical problems to integrate with legacy systems which are mostly based on relational database. In this paper, we model a large-scale topic map structure based on XTM 1.0 into RDB structure to minimize the processing time and build up the ontology in legacy systems. We implement a topic map platform called X-TOP that can enhance the efficiency of ontology construction and provide interoperability between XTM documents and database. Moreover, we can use conventional SQL tools and other application development tools for topic map construction in X-TOP. The X-TOP is implemented to have 3-tier architecture to support flexible user interfaces and diverse DBMS. This paper shows the usability of X-TOP by means of the comparison with conventional tools and the application to healthcare cancer ontology management.

• The Journal of the Korea Contents Association
• /
• v.18 no.7
• /
• pp.262-271
• /
• 2018

Recently, Daejeon Metropolitan City is planning a futuristic city with Daedeok Special District at the center of its transformation into a city of nature, science and culture along with the fourth industrial revolution. Therefore, this research will divide the 1.5 km distance from the in Doryong-dong, Daejeon, science park intersection to the (Former)Daedeok Science and Culture Center, and propose a space design concept that incorporates IT technologies that fit the characteristics. The center will analyze the space status and derive an experience-oriented space design concept for user-centered planning so that it can serve as a guideline that will provide the fundamental direction for the promotion of the Daedeok Science Culture. Through the field survey, we will look for ways to integrate IT technology with infrastructure that needs improvement and change the distance of Daedeok Science culture as a smart street. The results of the field survey were : (1) made by connecting the persistence of comfort and safety issues, (2) uniformity and efficiency issues, and (3) the experience of identity problems and the orientation of the smart city. To create user-centered distances, we attempted to avoid uniform zoning based solely on infrastructure and zoning focusing on user behavior.

• The Journal of Industrial Distribution & Business
• /
• v.10 no.8
• /
• pp.33-44
• /
• 2019

Purpose - As the scope of existing digital transformation expanded to various degrees, the Fourth Industrial Revolution came into being. In 2016, Klaus Schwab, Chairman of the World Economic Forum (WEF), said that the new technologies that lead the fourth industrial revolution are AI, Block chain, IoT, Big Data, Augmented Reality, and Virtual Reality. This technology is expected to be a full-fledged fusion of digital, biological and physical boundaries. Everything in the world is connected to the online network, and the trend of 'block chain' technology is getting attention because it is a core technology for realizing a super connective society. If the block chain is commercialized at the World Knowledge Forum (WKF), it will be a platform that can be applied to the entire industry. The block chain is rapidly evolving around the financial sector, and the impact of block chains on logistics, medical services, and public services has increased beyond the financial sector. Research design, data, and methodology - Figure analysis of data and social science analytical software of IBM SPSS AMOS 23.0 and IBM Statistics 23.0 were used for all the data researched. Data were collected from hotel employees in China from 25th March to 10th May. Results - The purpose of this study is to investigate the effect of the block chain characteristics of the existing hotel reservation system on the intention to use and to examine the influence of the block chain characteristics of the hotel reservation system on the intention to use, We rearranged the variables having the same or similar meaning and analyzed the effect of these factors on the intention to use the block chain characteristic of the hotel reservation system. 339 questionnaires were used for analysis. Conclusions - There are only sample hotel workers in this study, and their ages are in their 20s and 30s. In future studies, samples should be constructed in various layers and studied. In this study, the block chain characteristics are set as five variables as security, reliability, economical efficiency, availability, and diversity. Among them, Security and reliability made positive effects on the perceived usefulness. Also, security and economics did on the perceived ease. Availability and diversity did on both perceived usefulness and perceived ease. Perceived ease did on perceived usefulness. And perceived ease and perceived usefulness did on user intent. But security and economics did not on the perceived usefulness

• KIISE Transactions on Computing Practices
• /
• v.23 no.6
• /
• pp.359-370
• /
• 2017

In overseas-hospital construction, the digital hospital is a trend that is based on the developments of the information and communication technologies, state-of-the-art medical equipment, smart health, and telemedicine. Along with the increasing proportion of IT, this has resulted in the spreading of the concept throughout city-like hospitals and their transformation into digital hospitals. In the hospital-construction business, IT is a key element that will link the modernization of the mechanical, electrical, and equipment systems, construction, and medical equipment for efficiency maximization through integration. The purpose of this study is the analysis of the market-expansion success factors through the construction of a success-story-based, IT-driven overseas-hospital business. The digital-hospital concept and the development process are analyzed through a literature review, and the success factors are analyzed in terms of the cost, time, and quality that are proposed in the project-management body of knowledge. The main contributions of this study regarding the success factors are as follows: First, a cost-side need exists regarding the establishment of strategic-value engineering in terms of increasing the value from the perspectives of the IT and operational infrastructures; second, in terms of the construction time, all of the hospital systems must comply with the established deadlines for the integrated test and commissioning; and lastly, in terms of quality, it is important to ensure that the System Integration digital-hospital services are delivered according to the user requirements.