• Journal of Periodontal and Implant Science
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• 제38권1호
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• pp.41-50
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• 2008

Purpose: Bone morphogenetic protein-2(BMP-2) has been shown to possess significant osteoinducitve potential. There have been attempts to overcome a limitation of mass production, and economical efficiency of BMP. The aim of this study was to produce recombinant human BMP-2(rhBMP-2) from E. coli in a large scale and evaluate its biological activity. Materials and Methods: The E.coli strain BL21(DE3) was used as a host for rhBMP-2 production. Dimerized rhBMP-2 was purified by affinity chromatography using Heparin column. To determine the physicochemical properties of the rhBMP-2 expressed in E. coli, we examined the HPLC profile and performed Western blot analysis. The effect of the purified rhBMP-2 dimer on osteoblast differentiation was examined by alkaline phosphatase (ALP) activity and representing morphological change using C2C12 cell. Results: E. coli was genetically engineered to produce rhBMP-2 in a non-active aggregated form. We have established a method which involves refolding and purifying a folded rhBMP-2 dimer from non-active aggregates. The purified rhBMP-2 homodimer was characterized by SDS-PAGE as molecular weight of about 28kDa and eluted at 34% acetonitrile, 13.27 min(retention time) in the HPLC profile and detected at Western blot. The purified rhBMP-2 dimer stimulated ALP activity and induced the transformation from myogenic differentiation to osteogenic differentiation. Conclusion: rhBMP-2 was produced in E. coli using genetic engineering. The purified rhBMP-2 dimer stimulated ALP activity and induced the osteogenic differentiation of C2C12 cells.

• 안보군사학연구
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• 통권8호
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• pp.351-381
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• 2010

Established on February 1st, 2010, The ROKN maritime task flotilla has a goal to successfully cope with North Korea and other potential threats. Unlike precedents of its kind, The ROKN maritime task flotilla is expected to concentrate more an far-sea strategies than coastal strategies. In such circumstances however, the existing maritime logistics systems have limitations upon which to utterly support the operation conception of the ROKN maritime task flotilla seven. To ensure it's efficiency, transformation of the systems is prerequisite. The purpose of this study is to deduce an adequate maritime logistics system for the ROKN maritime task flotilla by evaluating the limitation of the current system and foreseeing its performance necessary abilities to operate in the future. In order to do this, the study presented problems and the direction of development on the structural and behavioral sides. Fist, regarding problems in the structure of maritime logistics system, the investigation pronounced the need of improvement in mobile logistics support ability and its speed. In addition, for the behavioral side, the need of improvement in logistics cooperation system and self-maintenance ability was affirmed. For the development of an adequate maritime logistics system, the study suggested the amelioration in the operation of an adequate mobile logistics support platform and the speed of the logistics using high-speed transportation such as planes for the structural side. Moreover, for the behavioral side, it recommended th enhancement of logistics cooperation system including the nations that already imported th weapon system, amelioration of self-maintenance ability, and necessity to operate mobile maintenance group.

• 농업과학연구
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• 제29권1호
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• pp.55-65
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• 2002

The most representative fruit in Yanbian area is Applepear(Ping Guo Li). It has been a important resource of farm income. According to the economic growth and income Increase of individual consumers, food consumption pattern will be changed from grain to high value cash crops such as Applepear and vegetables. The globalization and adapt ion of free market oriented economic policy of China government have led to change the collective farming system to individual farm management system. The institutional transformation have brought about high productivity of farm products and incomes of farmers. Therefore the plantation area of Applepear and requirement of investment cost for establishment of Applepear farms have increased rapidly since the 1980's, the time of perestroika and glasnost in Chinese economy. In prosperity of Chinese agriculture, individual farmer's decision making as free selection of farmland use, selection of high pay-off crops, free sale of the products in free market and credit support for the project might be the most important factors. In case of Applepear farm development, net present value of the project net benefit was estimated at 55.518won per hectare and financial rate of return was 21%. The benefit/cost ratio of the project was 2.11. Considering 10% of discounting rate or the opportunity cost of capital in China the Applepear farm development project showed us economically feasible in the light of the above efficiency indices. The Chinese government has to support Applepear farm development project financially and institutionally considering the high-payoff benefit of the fruit and farmer's in come increase in the future.

• 한국컴퓨터정보학회논문지
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• 제16권3호
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• pp.89-97
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• 2011

네크워크상의 분산되어 있는 정보를 접근하는 온톨로지와 같은 시맨틱 웹 정보 시스템에서는 효율적인 질의 처리를 위하여 질의 응답 시간을 줄여주는 향상된 캐쉬 메카니즘을 필요로 한다. 특히, P2P 네트워크 시스템은 웹 환경의 기본적인 하부 구조를 이루고 있으며, 질의가 발생하면, 소스 피어(Peer)로의 데이터 전송량을 줄이는 문제가 효율적인 질의 처리의 중요한 부분이다. 전통적인 데이터베이스 캐쉬 메카니즘으로부터 현재의 웹 환경에 적합한 질의 메카니즘들이 연구되어 왔으며, 질의 처리 결과를 캐쉬하는 것은 입력 질의 요구를 빠른 시간에 바로 사용자에게 전달할 수 있다. 웹 환경에서는 시맨틱 캐싱 방법이 연구되어 왔으며, 이는 캐쉬를 의미적인 영역들로 이루어진 공간으로 관리하는 개념이며, 논리적인 캐싱 단위가 질의와 질의 결과이므로 웹 환경에서 적합한 개념이다. 본 연구에서는 온톨로지와 같은 시맨틱 웹 정보가 클러스터 단위로 여러 피어에 분산되어 있는 경우에 캐쉬 메카니즘을 이용하여 효율적인 질의 처리가 이루어지도록 하는 방법을 제시한다. 특히, 캐쉬를 유지하고 처리하는 방법으로 스키마를 이용한 캐쉬 데이터 필터링 방법과 온톨로지와 질의 결과의 유사도를 측정하여 캐쉬 대체 영역 선택에 사용하는 방법을 제시한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 하계학술대회 논문집 B
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• pp.1489-1491
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• 2004

A guard lamp system has been installed at the PV positive center, located at Gwangju in Korea. Digital environment that is represented to internet is displacing business way of industry and business achievement way with the fast speed being giving great change on life whole, improve existence business process utilizing internet and Web connection technology, information superhighway to tradition industrialist manufacture and e-transformation's propulsion that wish to maximize productivity and administration efficiency is spread vigorously. In this paper, we wish to accomplish generation equipment's heighten stability and believability through remote monitoring and control of guard lamp system. This paper describes the design of the monitoring system for the sensing data and indirect controlling of the guard lamp system. Most of the conventional monitoring systems depend on the special hardware and software. The essential design of monitoring system is to provide the convenience for the user and the portability for the system. In order for the system to fulfill its requirements, it was designed using Labview GUI facility based on the Windows 2000 environment of IBM PC compatible and Add-oncard based on the TCP/IP Protocol. Advantage of the monitoring system are a personnel expenses curtailment effect, of the place restriction and unmanned system of the generationplants, etc..

• 한국미생물·생명공학회지
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• 제16권6호
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• pp.476-483
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• 1988

효모 유전자 운반체의 유형을 특징지우는 ARS1 (autonomous replicating sequence), CEN3 (centromere), 2 $\mu$m OR (yeast plasmid의 origin of replication)의 DNA 절편을 재조합하여 만든 유전자 운반체들의 형질전환력, 안정도 및 운반체 수를 효모균주 SHY4(cir$^+$)와 NNY1(cir$^{\circ}$)에서 비교 조사하였다. CEN3를 갖는 유전자 운반체는 매우 안정하나 세포당 수가 하나로 매우 낮으며 2 $\mu$m OR과 ARS1 을 동시에 갖는 유전자 운반체는 안정할 뿐만 아니라 세포당 수도 높으며 균주내에 존재하는 2 $\mu$m 플라스미드는 2 $\mu$m OR을 가지는 유전자 운반체의 복제에 영향을 준다는 사실을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.784-791
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• 2007

The present work aims to use a biofilter technology(aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water(OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days($BOD_5$) and chemical oxygen demand(COD), decreased markedly(up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced(around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the $\alpha-subclass$ of Proteobacteria, and often branched in the periphery of bacteria] genera commonly present in soil(Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.101-101
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• 2010

ZnO shows a direct band gap of 3.37eV, large exciton binding energy (~60 meV), high oxidation ability, high sensitivity to many gases, and low cost, and it has been used in various applications such as transparent electrodes, light emitting diodes (LEDs), gas sensors and photocatalysts. Among these applications ZnO as photocatalyst has considerably attracted attention over the past few years because of its high activities in removing organic contaminants generated from industrial activities. In this research, ZnO nanoparticles were synthesized by spray-pyrolysis method using the zinc acetate dihydrate as starting material at synthesis temperature of $900^{\circ}C$ with concentration varied from 0.01 to 1.0M. The physical and chemical properties of the synthesized ZnO nanoparticles were examined by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier Transformation Infrared (FT-IR), and UV-vis spectroscopy. The Miller indices of XRD patterns indicate that the synthesized ZnO nanoparticles showed a hexagonal wurtzite structure. With increased precursor concentration, a primary, secondary particle sizes of ZnO nanoparticles increased by 0.8 to $1.5{\mu}m$ and 15 to 35nm, and their crystallinity was improved. Methyleneblue (MB) solution ($1{\mu}M$) as a test comtaminant was prepared for evaluating the photocatalytic activities of ZnO nanoparticles synthesized in different precursor concentration. The results show that the photocatalytic efficiency of ZnO nanoparticles was gradually enhanced by increased precursor concentration.

• 대한기계학회논문집A
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• 제36권9호
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• pp.1003-1008
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• 2012

실시간 차량동역학 해석을 위해서는 효율적인 차량 모델이 필요하게 된다. 효율성을 높이기 위해 집중질량모델로 가정하면 현가장치의 특성을 고려하기 어렵게 되며, 현가장치의 특성을 모두 고려한 다물체동역학 모델에서는 효율성이 떨어진다. 그러므로 본 논문에서는 다물체동역학 모델링을 사용하되 해석의 효율성을 저하시키는 현가장치의 각종 요소들의 효과는 기구정역학 실험으로 추출된 특성그래프로 대체함으로써 효율성도 기하고자 시도하였다. $6{\times}6$ 차량을 차체와 휠로 구성된 차량으로 모델을 정의하였고, 다물체동역학 모델인 ADAMS 결과와 비교하여 실험적 모델의 유용성을 검증하였다. 그리고 검증된 실험적 차량모델을 RT-LAB을 활용한 실시간 시뮬레이션 환경에 삽입하여, 실시간성 시뮬레이션의 가능성을 검증하였다.

• 한국융합학회논문지
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• 제12권9호
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• pp.39-47
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• 2021

본 연구에서는 계절적인 요인과 급변하는 상품의 트렌드를 사전예측하기 위해 로지스틱 회귀 알고리즘을 이용한 상품기획 예측 모형을 제안하고자 수행되었다. 먼저 웹크롤링을 이용하여 포털 사이트 및 온라인 마켓의 소비자의 비정형 데이터를 수집하고 정형 데이터 변환을 위한 전처리 작업을 통해 상품에 대한 의미 있는 정보를 분석하였다. 최종 수집된 11,200개의 데이터셋은 Logistic Regression을 이용하여 상품에 대한 소비자의 만족도, 빈도분석, 상품에 대한 장점과 단점을 분석할 수 있었다. 분석 결과 소비자의 만족도는 92%이었으며, 빈도분석을 통해 상품에 대한 불량이슈를 확인할 수 있었다. 또한, 개발된 상품 기획 예측 프로그램에 대한 사용 만족도, 시스템 효율성, 시스템 효과성 항목에 대한 분석결과에서도 만족도가 높게 나타났다. 특히, 불량이슈는 상품에 대한 현 문제를 신속히 인지하고 개선 전략을 수립하는데 필요한 정보를 제공한다는 점에서 매우 의미 있는 자료가 된다.