• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.72-72
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• 2003

Induction of glutathione S-transferases is associated with cancer chemoprevention. We reported that PD98059, an MKK1 inhibitor, induces glutathione Stransferase A2 (rGSTA2). This report comparatively examines the role of CCAAT/enhancer binding protein (C/EBP) and Nrf-2 in the induction of rGSTA2 by PD98059. PD98059 at the concentrations effective for the inhibition of MKKI increased the rGSTA2 protein and mRNA levels in H4IIE cells. (omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 춘계학술대회 논문집
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• pp.60-61
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• 2003

Effects of repeated treatment with butylated hydroxyanisole (BRA) on the induction of glutathione S-transferases (GSTs) and teratogenicity of cyclophosphamide were investigated in rats. Pregnant rats were orally treated with BRA (50 mg/kg) for 7 days, from days 6 to 12 of gestation, and subcutaneously challenged with cyclophosphamide (15 mg/kg) 2 hr after the final treatment. On day 20 of gestation, the maternal and fetal abnormalities were examined.(omitted)

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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• pp.68-68
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• 2003

The glutathione-S-transferases (GSTs) are enzymes responsible for the protection of cells from chemical toxicants and oxidative stress. In insects, GSTs have been particularly known to be implicated in the resistance to insecticides. In this study, a cDNA encoding the GST gene homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. (omitted)

• Mycobiology
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• 제45권3호
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• pp.226-231
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• 2017

Coprinopsis cinerea was employed to investigate the fungal response to gravity. Mycelium growth revealed a consistent growth pattern, irrespective of the direction of gravity (i.e., horizontal vs. perpendicular). However, the fruiting body grew in the direction opposite to that of gravity once the primordia had formed. For the proteomic analysis, only curved-stem samples were used. Fifty-one proteins were identified and classified into 13 groups according to function. The major functional groups were hydrolases and transferases (16%), signal transduction (15%), oxidoreductases and isomerases (11%), carbohydrate metabolism (9%), and transport (5%). To the best of our knowledge, this is the first report on a proteomic approach to evaluate the molecular response of C. cinerea to gravity.

• Journal of Ginseng Research
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• 제24권2호
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• pp.94-98
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• 2000

Tetracycline hydrochloride (TC) caused 100%, 50% and 20% mortality rates among rats injected with 40 mg, 30 mg and 20 mg/100g. b.w. respectively; while the morta]ity rates were decreased to 50%, 20% and 10% when Panax ginseng (2 mg/100g. b.w.) injected with TC during 72 hrs. post-injection. Subacute-toxicity study demonstrated that TC caused severe hepato-nephrotoxicity (demonstrated by biochemical analysis of serum including: transferases , alkaline phosphatase, total protein, glucose, cholesterol urea and creatinine) in rats injected i.p. with 10 mg and 5 mg/100g. b.w. for 7 days of daily injection . These signes of toxicity were greatly diminished by P. ginseng addition to TC doses.

• 약학회지
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• 제47권4호
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• pp.218-223
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• 2003

Effect of taurine treatment on metabolism of glutathione (GSH) was studied in adult male ICR mice. An acute injection of taurine (250 mg/kg, ip) resulted in a significant decline of hepatic GSH level at t = 6 hr, but plasma GSH level was not altered. The activity of GSH-related enzyme in liver, such as GSH peroxidase, GSSG reductase, GSH S-transferases, ${\gamma}$-glutamylcysteine synthetase or ${\gamma}$-glutamyltranspeptidase, was not affected by taurine at t = 2.5 or 6 hr. Plasma cysteine and cystine levels were elevated rapidly following taurine treatment. Hepatic cysteine level was decreased by taurine, reaching a level approximately 70％ of control at t = 4 and 6 hr. In conclusion, the results indicate that an acute dose of taurine decreases hepatic GSH level by reducing the availability of cysteine, an essential substrate for synthesis of this tripeptide in liver. It is also suggested that taurine may decrease the cysteine uptake by competing with this S-amino acid for a non-specific amino acid transporter.

• Archives of Pharmacal Research
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• 제22권6호
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• pp.533-541
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• 1999

Many conventional anticancer drugs display relatively poor selectivity for neoplastic cells, in particular for solid tumors. Furthermore, expression or development of drug resistance, increased glutathione transferases as well as enhanced DNA repair decrease the efficacy of these drugs. Research efforts continue to overcome these problems by understanding these mechanisms and by developing more effective anticancer drugs. Cyclophosphamide is one of the most widely used alkylating anticancer agents. Because of its unique activation mechanism, numerous bioreversible prodrugs of phosphramide mustard, the active species of cyclophosphamide, have been investigated in an attempt to improve the therapeutic index. Solid tumors are particularly resistant to radiation and chemotherapy. There has been considerable interest in designing drugs selective for hypoxic environments prevalent in solid tumors. Much of the work had been centered on nitroheterocyclics that utilize nitroreductase enzyme systems for their activation. In this article, recent developments of anticancer prodrug design are described with a particular emphasis on exploitation of selective metabolic processes for their activation.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.82.1-82.1
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• 2003

The protective adaptive response to electrophiles and reactive oxygen species is mediated by enhanced expression of phase II detoxifying genes including glutathione S-transferases. ${\alpha}$-Lipoic acid, which exerts prooxidant or antioxidant activities, has been shown to activate the insulin signaling pathway and thus to induce insulin-like actions via PI3-kinase and Akt. Our previous studies have shown that PI3-kinase plays an essential role in Nrf2-or C/EBP${\beta}$-mediated glutathione S-transferase A2 (GSTA2) induction. (omitted)

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.531-534
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• 2023

We report the draft genome sequence of the yeast strain Hormonema macrosporum POB-4, capable of producing the biosurfactant glycocholic acid, one of the bile acids. A majority of genes with known function were associated with metabolism and transport of amino acid and carbohydrate as well as secondary metabolites biosynthesis, transport, and catabolism. We observed genes of eleven C-N hydrolases and two CoA transferases which have been reported to be involved in the biosynthesis of glycocholic acid. Further experimental studies can help to elucidate the specific genes responsible for biosurfactant production in strain POB-4.

• 한국환경농학회지
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• 제8권1호
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• pp.47-54
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• 1989

동양종(東洋種)꿀벌 (Apis cerana F.)에 대(對)한 살충제(殺蟲劑)의 독성(毒性) 및 해독능력(解毒能力)을 조사(調査)하고 농약한계 사용량 결정에 기여하기 위하여 7가지 대표적인 살충제의 꿀벌에 대한 독성 및 해독효소의 활성을 조사하였다. 효소 활성은 해독효소로 알려진 microsomal oxidases, glutathione S-transferasecs, esterase와 DDT-dehydrochlorinase를 조사했고 성충(成蟲)일벌의 중장(中腸)을 사용하여 측정하였다. $LC_{50}$치의 측정 결과는 다음과 같다. 1. 공시 살충제중 DDT가 19ppm으로 독성(毒性)이 가장 낮았고 EPN이 0.75ppm으로 독성(毒性)이 가장 강(强)했다. 2. 준치사농도(準致死濃度)의 농약(農藥)이 성충(成蟲)일벌의 microsomal oxidase에 미치는 영향은 malathion 및 demeton S-methyl 처리가 aldrin epoxidase활성을 저해시켰고 N-demethylase활성은 carbayl 처리구에서 증대(增大)되었다. 3. Glutathione S-transferase(DCNB conjugation)활성은 diazinon과 malathion처리구에서 증대되었다. 4. Esterase는 malathion 및 permethrin처리구에서 ${\alpha}-NA$ esterase 활성(活性)의 저해(沮害)를 보였고 carboxylesterase와 AchE 활성은 거의 영향이 없었다. 5. DDT-dehydrochlorinase 활성은 carbaryl, malathion과 demeton S-methyl 처리구에서 저해를 보였다.