• 한국수정란이식학회지
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• 제27권1호
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• pp.1-7
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• 2012

Somatic cell nuclear transfer (SCNT) for miniature pig has been developed for xenotransplantation and many other biomedical experiments. However, the efficiency of SCNT is still very low due to many factors. To optimize the surrogate mother condition for improvement of cloned miniature pigs efficiency, we investigated the effect of the status of surrogate mother on pregnancy, farrowed rate in SCNT pigs. After SCNT with mesenchymal stem cells as donor cells, the SCNT embryos were surgically transferred into the oviduct of surrogated pigs. To compare the effects of status of surrogate pigs on pregnancy, surrogate pigs were prepared by artificial abortion at day 20~29 (Group 1), 30~39 (Group 2), and 40~45 (Group 3) of gestation. After SCNT embryos transfer in three different status of surrogate pigs, Group 2 (56.3%) and 3 (55.6%) had significantly ($p$ <0.05) higher the pregnancy rate than group 1 (0%) at day 30 of gestation. The status of ovulation in surrogate pig also was investigated. Post-ovulation status (54.8%) had higher proportion than pre-ovulation status (38.7%) and ovulation status (6.5%). We obtained 19 cloned miniature piglets from seven surrogate gilts and five piglets are living healthy but fourteen piglets died soon after birth or stillbirth. The weights of piglets greatly differ from 254 to 1,296 g. Microsatellite analysis showed that cloned piglets were genetically different from the surrogate mother and cloned piglets were genetically equal to the donor cell. In conclusion, the present result indicates that artificially abortion method can improve the efficiency of pregnancy after SCNT in pigs. This study will provide available method for the further study and application in the field of xenotransplantation.

• 한국수정란이식학회지
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• 제15권3호
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• pp.255-261
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• 2000

The objective of this study was to improve the efficiency of bovine embryo transfer by transferring of Hanwoo embryos into Hanwoo or Holstein recipients. The cryopreserved or fresh in vitro produced(IVP) embryos were transferred into uterine horn contralaterally or ipsilaterally to the corpus luteum. The recipients were inseminated by artificially on the next day of estrus. The pregnancy was diagnosed by rectal palpation at 60∼90 days after transfer of the embryos. The pregnancy rate by transfer of one or two embryos was 78%(7/9) and 74%(31/42), respectively. The pregnancy rates according to the grade of corpus lutea of recipients was 75% (20/27) and 82.0%(18/22) at the grade of A and B, respectively. Ten(67.0%) of 15 Holstein recipients transferred with IVP Hanwoo embryos and 5(42.0%) of 12 Holstein recipients transferred with frozen IVP Hanwoo embryos were pregnant. The single and twin calving ratio in Hanwoos was 77.0%(10/13) and 23.0%(3.13) in the recipients transferred with IVP embryos and 64.0%(7/10) and 27.0%(3/10) in the recipients transferred with frozen IVP embryos, respectively. Twenty-four pregnant cows following transfer of IVP embryos, 21(88.0%) calved the normal calves, and 2(8.3%) aborted. When the frozen IVP embryos were transferred, 16 pregnant cows calved 14(88.0%) normal calves and 2(13.0%) aborted. In conclusion, when one or two IVP bovine embryos were transferred into recipients, the A and B grade of corpus luteum resulted in high pregnancy rates. For the production of twin calves, transfer of the IVP or frozen IVP embryos could be suitable.

• 전자공학회논문지
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• 제54권7호
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• pp.49-54
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• 2017

본 연구에서 페라이트를 이용한 자기장 무선전력전송 안테나를 제안하였다. 기존에 추가접지가 있어야 자기장 무선전력전송이 가능하였던 점을 페라이트의 특성을 이용하여 추가접지 없이 자기장 무선전력전송이 가능하게 하였다. 페라이트는 자기장의 차폐효과가 있고 누설전력을 줄여주어 전송효율을 높여준다. 페라이트를 이용하여 자기장 무선전력전송을 위한 안테나를 제안하였고 실험을 통해 5W 자기장 무선전력전송 되는 것을 확인하였다. 본 논문에서 제안한 무선전력전송은 금속을 매개로하는 자기장 무선전력전송으로 사물인터넷 분야에 다양하게 응용 될 수 있다.

• 설비공학논문집
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• 제28권3호
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• pp.95-102
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• 2016

A boiling heat transfer is used in various industry such as power generation systems, heat exchangers, air-conditioning and refrigerations. In the boiling heat transfer system, the critical heat flux (CHF) is the important factor, and it indicated safety of the system. It has kept up studies on the CHF enhancement. Recently, it is reported the CHF enhancement, when working fluid used the nanofluid with high thermal properties. But it could be occurred nanoflouling phenomenon from nanoparticle deposition, when nanofluid applied the heat transfer system. And, it is reported that the safety and thermal efficiency of heat transfer system could decrease. Therefore, it is compared and analyzed to the CHF and the boiling heat transfer coefficient on effect of artificial nanofouling (coating) in oxidized multi-wall carbon nanotube nanofluids. As the result, the CHF of oxidized multi-wall carbon nanofluids and the CHF of artificial nanofouling in the nanofluids increased to maximum 99.2%, 120.88%, respectively. A boiling heat transfer coefficient in nanofluid increased to maximum 24.29% higher than purewater, but artificial nanofouling decreased to maximum -7.96%.

• 신재생에너지
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• 제18권2호
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• pp.73-81
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• 2022

This study was aimed at designing a condenser, as a component of the organic Rankine cycle system for ships. The condenser was manufactured through press molding to achieve a bent shape to enhance the heat transfer performance, considering the shape of the heat transfer plate used in a brazing plate heat exchanger. The heat transfer plate was made of copper-nickel alloy. The required heat transfer rate for the condenser was 110 kW, and the maximum number of layers was set as 25, considering the characteristics of high-temperature brazing. Computational fluid dynamics techniques were used to perform the thermal fluid analysis, based on the ANSYS CFX (v.18.1) commercial program. The heat transfer rate of the condenser was 4.96 kW for one layer (width and length of 0.224 and 0.7 m, respectively) of the heat transfer exchanger. The fin efficiency pertaining to the heat transfer plate was approximately 20%. The heat flow analysis for one layer of the heat exchanger plate indicated that the condenser with 25 layers of heat transfer plates could achieve a heat transfer rate of 110 kW.

• 한국항공우주학회지
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• 제35권12호
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• pp.1143-1150
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• 2007

스마트무인기 연료공급시스템에 사용되는 제트펌프의 연료탱크 사이의 연료이송 특성이 실험적으로 조사되었다. 제트펌프의 작동조건은 비행체의 임무에 따른 엔진의 요구사항을 만족하도록 설정되었으며, 주어진 조건에서 제트펌프의 성능을 평가하였다. 제트펌프의 유량비 변화에 따른 압력비 및 효율이 측정되었으며 면적비 변화 등이 고려되었다. 평가 결과로부터 제트펌프는 주어진 압력비보다 큰 범위에서 작동목표인 유량비 2.23을 만족하였다. 효율보다 연료공급량이 중시되는 연료이송 제트펌프의 경우 면적비 변화를 통하여 허용 압력비 범위 내에서 유량비 조절이 가능함을 알 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제26권4호
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• pp.537-542
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• 2005

It was proposed that Ru(II)[(1,10-phenanthroline)$_2$dipyrido[3,2-a:2',3'-c]phenazine ([Ru(phen)$_2$DPPZ]$^{2+}$)complexes and 4',6-diamidino-2-phenylindole (DAPI) simultaneously bind to poly[d(A-T)$_2$] (Biophysics. J. 2003, 85, 3865). Förster type resonance energy transfer from excited DAPI to [Ru(phen)2DPPZ]$^{2+}$ complexes was observed. In this study, we synthesized $\Delta$- and $\wedge$-[Ru(phenanthroline)$_2$dipyrido[3,2-a:2’3’c]6-azaphenazine] ([Ru(phen)$_2$DPAPZ]$^{2+}$) at which the DNA intercalating ligand DPPZ was replaced and we studied its binding properties to poly[d(A-T)$_2$] in the presence and absence of DAPI using polarized spectroscopy and fluorescence techniques. All the spectroscopic properties of the [Ru(phen)$_2$DPAPZ]$^{2+}$-poly[d(A-T)$_2$] complex were the same in the presence and absence of DAPI that blocks the minor groove of polynucleotide, suggesting both $\Delta$- and $\wedge$-[Ru(phen)$_2$DPAPZ]$^{2+}$ complexes are located at the major groove of poly[d(A-T)2]. On the other hand, in contrast with [Ru(phen)$_2$DPPZ]$^{2+}$, both $\Delta$- and $\wedge$-[Ru(phen)$_2$DPAPZ]$^{2+}$ exhibited almost twice the efficiency in the fluorescence quenching of DAPI that binds at the minor groove of poly[d(A-T)$_2$]. This observation indicates that the efficiency of the Förster type resonance energy transfer can be controlled by a small change in the chemical structure of the intercalated ligand.

• 한국전자파학회논문지
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• 제26권5호
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• pp.521-524
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• 2015

본 논문에서는 재구성이 가능한 다중포트 전력증폭기를 이용해 선택적으로 무선 전력 전송을 할 수 있는 구조를 제안한다. 제안된 무선 전력 전송 구조는 FPGA에 의해 제어되는 입력 위상 가변부, 두 개의 Class-E급 전력증폭기, 4-포트 직교전력 결합기, 두 개의 부하 코일로 구성된다. FPGA에 의해 제어되는 입력 위상부에 의해 두 코일에 전력이 선택적으로 1:1, 2:0, 0:2의 비율로 분배된다. 제작한 시스템은 측정 결과, 125 kHz에서 1 W DC 전력을 전달하였다. 각 개별 전력증폭기는 79 % 효율을 가졌으며, 정류변환을 포함한 최종 DC-DC 변환효율은 40 % 이상을 보였다.

• 대한전자공학회논문지SD
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• 제46권12호
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• pp.80-86
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• 2009

전하펌프의 성능은 공급전압에 의해 크게 영향을 받는다. 본 논문에서는 표준 twin-well CMOS 로직 공정으로 제작 가능하며, 낮은 공급전압에서도 높은 효율을 갖는 새로운 전하펌프 회로를 제안하고 검증하였다. 제안한 전하펌프는 이중의 전하 전달 경로와 간단한 2-phase 클락을 사용한다. 한 주기의 펌핑 사이클 동안 각 펌핑 단에서 입력전압을 2배로 승압하며, 상보적으로 연결된 PMOS 트랜지스터를 전달 스위치로 사용하여 트랜지스터의 문턱전압에 의한 전압강하 없이 승압된 전압을 다음 승압 단으로 전달한다. 시뮬레이션과 측정을 통해 제안한 전하펌프를 검증하였으며, 동일한 공정조건에서 제작 가능한 기존 전하펌프들 보다 높은 출력전압과 큰 전류 구동능력 그리고 더 높은 전력효율을 가진다는 것을 확인하였다.

• 한국발생생물학회지:발생과생식
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• 제19권2호
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• pp.79-84
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• 2015

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.