• 한국임상수의학회지
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• 제37권5호
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• pp.261-269
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• 2020

The purpose of this study is to investigate the clinical effects of carboxymethyl chitosan (CMC) and adipose-derived mesenchymal stem cells (MSCs) on osteoarthritis (OA). Thirty New Zealand white rabbits were used as cranial cruciate ligament transection and partial meniscectomy models. The rabbits were divided into five groups (n = 6) according to the intra-articular injection materials: the control group with PBS, the HA group with hyaluronic acid, the CMC group with CMC, the MSC group with MSCs emerged in PBS, and the MSC+CMC group with CMC and MSCs. Knee thickness, extension angle, gross morphology, histopathology and immunohistochemistry were performed to evaluate the effects of CMC and MSCs on rabbit OA. On the morphologic and histologic examination, the articular surfaces of the femur and tibia were markedly damaged in control group with higher Mankin score and lower cartilage surface thickness. However, OA related cartilage defects were alleviated by the treatment of MSC and/or CMC. The expressions of apoptotic and inflammatory cytokines were decreased and cartilage extracellular matrix (ECM) related collagens I and II were enhanced by the treatment of MSC and/or CMC. In conclusion, this study showed that CMC and MSC treatments have a beneficial effects on OA via the protection of cartilage damage, the stimulation of ECM, and the inhibition of inflammatory and apoptotic reaction.

• 상하수도학회지
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• 제21권1호
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• pp.57-63
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• 2007

In this study, the cone type baffle (CTB) was developed to improve the flow distribution in an upflow bioreactor. It was composed of two different zones (anoxic and oxic) by inserting the CTB in the middle of the bioreactor. Based on the results of the dye tracer tests, hydraulic retention time (HRT) for the anoxic, oxic and total volume of the bioreactor was found to be 0.998, 1.996 and 2.994 hr, respectively. And the theoretical HRT for each volume was 1.0, 2.0 and 3.0 hr, respectively. The values of HRT obtained from the tests coincide with theoretical values. Therefore, the flow pattern for each zone inside the bioreactor, which was divided by the CTB, was dominated by complete mix flow rather than plug flow. Based on the results of the transection measurements, the DO concentration for each zone inside the bioreactor was clearly distinguished. Consequently, the CTB played an important role in the separation of anoxic and oxic zones. Also, the each pollutans were reduced gradually along the height in the oxic zone. The reason is that the biomass in the media layer (Port 4 and 5) was higher than that in the mixed liquor layer without the media (Port 2 and 3).

• 한방재활의학과학회지
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• 제18권4호
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• pp.13-24
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• 2008

Objectives : The purpose of this study was to investigate the effect of Poncirus Trifoliata(PT) on improvement of fecal impaction in spinal cord injured(SCI) rats. Methods : Fifteen adult Sprague-Dawley female rats were used weighing 200~250 g. A complete spinal cord transection was performed surgically at the T10 cord level. Experimental groups were assigned into 3 groups: Control(n=5), SCI+vehicle(n=5) and SCI+PT(n=5). PT was administered 100mg/kg in 0.5ml every 24 hours from 1st operation day to 7th day. We measured the body weight and food intake as well as the number and the weight of fecal pellet every morning. After 1 week of operation, whole colon was divided into proximal and distal segments under anesthesia. Each segment of colon was mounted with longitudinal direction in a organ bath. We measured spontaneous contraction and compared the area under the curve in each segments. Enhanced responses were observed by acetylcholine($10^{-6}M$), 40 mM KCl solution, L-NAME($10^{-4}M$). Results : The fecal number and weights were significantly higher in the group of SCI+PT than SCI+vehicle group(p<0.05). In organ bath study, area under the curves of the spontaneous contraction in SCI+vehicle and SCI+PT groups were significantly increased compared to control group. Contractility of distal colon in response to acetylcholine or KCl in SCI+vehicle group was significantly decreased compared to other groups(p<0.05). Conclusions : These results suggest that PT might be useful to promote bowel emptying in spinal cord injured rats.

• 한국조경학회지
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• 제30권3호
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• pp.73-85
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• 2002

The purpose of this study was to develop prediction models for plant species abundance by stream restoration. Generally the stream plant is affected by stream gemophology. So in this study, the relationship between the vegetation abundance and stream gemophology was developed by multiple regression analysis. The stream characteristics utilized in this study were longitudinal slope, transectional slope, micro-landforms through the longitudinal direction, riparian width and geometric mean diameter and biggest diameter of bed material, and cumulated coarse and fine sand weight portion. The Pyungchang River with mountainous watershed and the Kyungan stream and the Bokha stream in the agricultural region were selected and vegetation species abundance and stream characteristics were documented from the site at 2~3km intervals from the upper stream to the lower. The Models for predicting the vegetation abundance were developed by multiple regression analysis using SPSS statistics package. The linear relationship between the dependant(species abundance) and independant(stream characteristics) variables was tested by a graphical method. Longitudinal and transectional slope had a nonlinear relationship with species abundance. In the next step, the independance between the independant variables was tested and the correlation between independant and dependant variables was tested by the Pearson bivariate correlation test. The selected independant variables were transectional slope, riparian width, and cumulated fine sand weight portion. From the multiple regression analysis, the $R^2$for the Pyungchang river, Kyungan stream, Bokga stream were 0.651, 0.512 and 0.240 respectively. The natural stream configuration in the Pyungchang river had the best result and the lower $R^2$for Kyunan and Bokha stream were due to human impact which disturbed the natural ecosystem. The lowest $R^2$for the Bokha stream was due to the shifting sandy bed. If the stream bed is fugitive, the prediction model may not be valid. Using the multiple regression models, the vegetation abundance could be predicted with stream characteristics such as, transection slope, riaparian width, cumulated fine sand weigth portion, after stream restoration.

• Korean Journal of Acupuncture
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• 제23권3호
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• pp.55-71
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• 2006

Objectives : The present study was investigated the effect and pathway of heterotopic electroacupuncture (EA) on pain induced by formalin in rats. Methods : Acupoints in the right forepaws, $HT_7$ and $PC_7$, were stimulated with 3 mA, 2 ms, and 10 Hz before subcutaneously formalin injection (5%, $50{\mu}l$) to the left hind paw. Moreover, it was investigated whether the dorsolateral funiculus (DLF), as known to the descending inhibition, mediates analgesia of the heterotopic EA, and an administration of naltrexone blocks the effect of EA. Results : In the immunohistochemistry of cFos-like protein (cFL), there were inhibitory effects of EA on the increased expression of cFL in the lumbar spinal dorsal horn neurons following formalin injection. Especially, EA inhibited the expression of cFL on the superficial laminae than that on the deep laminae at 1 hr after, but that on the deep laminae than that on the superficial laminae at 2 hr after. Also, EA suppressed the increased expression of nitric oxide (NO) and neuronal nitric oxide synthase (nNOS) in the lumbosacral spinal cord after formalin injection, but not Sham-EA. Suppressed expressions of cFL, NO and nNOS in the spinal cord were eliminated after transection of the ipsilateral DLF at $T_{10}{\sim}T_{11}$ levels. However, pretreatment of naltrexone could not prevent the suppressive expressions of cFL, NO and nNOS at the spinal cord. Conclusions : These results suggest that the analgesia of heterotopic EA may be modulated through the DLF constituting the descending inhibition.

• The Korean Journal of Physiology
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• 제15권2호
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• pp.73-81
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• 1981

Experiments were conducted in ischemic decerebrate cats to study the effects of electroacupuncture and electrical stimulation of peripheral nerve on pain reaction. Flexion reflex was used as an index of pain. The reflex was elicited by stimulating the sural nerve(20 V, 0.5 msec duration) and recorded as a compound action potential from the nerve innervated to the semitendinosus muscle. Electroacupuncture was performed, using a 23-gauge hyperdermic needle, on the tsusanli point in the lateral upper tibia of the ipsilateral hindlimb. The common peroneal nerve was selected as a peripheral nerve which may be associated with electroacupuncture action, as it runs through the tissue portion under the tsusanli point. Both for electroacupuncture and the stimulation of common peroneal nerve a stimulus of 20 V-intensity, 2 msec-duration and 2 Hz-frequency was applied for 60 min. The results are summerized as follows: 1) The electroacupuncture markedly depressed the flexion reflex; this effect was eliminated by systemic application of naloxone $(0.02{\sim}0.12\;mg/kg)$, a specific narcotic antagonist. 2) Similarly, the electrical stimulation of the common peroneal nerve significantly depressed the flexion reflex, the effect being reversed by naloxone. 3) When most of the afferent nerves excluding sural nerve in the ipsilateral hindlimb were cut, the effect of electroacupuncture on the flexion reflex was not observed. Whereas direct stimulation of the common peroneal nerve at the proximal end from the cut resulted in a significant reduction of the flexion reflex, again the effect was reversible by naloxone application. 4) Transection of the spinal cord at the thoracic 12 did not eliminate the effect of peripheral nerve stimulation on the flexion reflex and its reversal by naloxone, although the effect was significantly less than that in the animal with spinal cord intact. These results suggest that: 1) the analgesic effect of an electroacupuncture is directly mediated by the nervous system and involves morphine-like substances in CNS, 2) the site of analgesic action of electroacupuncture resides mainly in the brainstem and in part in the spinal cord.

• 한국작물학회지
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• 제31권2호
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• pp.167-172
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• 1986

우리나라의 재래종 대화형 대두인 부채콩의 생육 및 형태적 특성을 정상형 대두인 단엽콩과 비교하였던 바 그 결과를 요약하면 다음과 같았다. 1. 대화형 대두인 부채콩의 주경은 생육이 진행됨에 따라 대화가 심해졌고 정상형인 단엽콩에 비해 출엽이 불규칙하였으며 주경엽수는 단엽콩보다 훨씬 증가하였으나 총엽수는 크게 다르지 않았다. 2. 부채중의 주경대화정도는 약 3.1정도였으며 대화정도는 정상형인 단엽콩의 주경과 분핵의 생육량과 유관하였다. 3. 부채콩의 분지수는 단엽콩보다 적었으나 분지가 대화하는 경우도 있었으며 엽병의 대화도 관찰되었다. 4. 부채콩은 협의 대부분이 주경 선단부에 집중 분포하였다. 5. 부채콩의 수량은 단엽콩의 60％정도였으며 수량감소의 주원인은 종실중과 협당입수의 감소였다. 6. 주경 횡단면의 유관속수는 부채콩이 많았으나 각 유관속은 단엽콩이 발달하였다.

• 한국문헌정보학회지
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• 제52권1호
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• pp.469-490
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• 2018

본 연구의 목적은 공공도서관 상호대차서비스 데이터를 이용하여 현재 수행되는 서비스의 현황을 분석하는 것이다. 또한 분석 과정에서 서비스 수행과 관련된 이슈를 발견하고 이를 개선하기 위한 방안을 제안하는 것을 목적으로 한다. 분석을 위해 책바다 서비스 데이터와 국가도서관통계시스템 데이터, 지역 단위 상호대차서비스 현황 데이터를 수집하여 분석하였다. 연구결과, 상호대차서비스 이용은 계속적으로 증가하고 있었고 특히 지역 단위에서 운영되는 상호대차 서비스가 활발히 수행되고 있음을 알 수 있었다. 또한 상호대차서비스 요청과 제공 등의 이용뿐만 아니라 서비스 네트워크에 참여하는 도서관들의 유형이 다양하고 그 숫자도 확대되고 있는 것으로 나타났다. 덧붙여, 상호대차서비스 네트워크에서 각 도서관이 어느 정도 협력하는지를 측정하기 위한 도구의 하나로 공헌도에 대한 논의를 진행하였다. 이용과 관련된 통계뿐만 아니라 개별 도서관의 장서적인 고유성, 도서 기반의 협업 지수 등을 고려한 공헌도 분석의 필요성을 제안하였다.

• Korean Journal of Acupuncture
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• 제36권1호
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• pp.52-62
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• 2019

Objectives : The objective of this study was to investigate the effects of Zingiberis Rhizoma Pharmacopuncture(ZP) at GB30 and ST36 in neuropathic pain induced SD rats by the block of Transient Receptor Potential Vanilloid 1(TRPV1). Methods : Neuropathic pain in rats was induced by tibial and common peroneal nerve transection of right leg. The rat subjects were divided into 6 groups : normal(Nor, n=5), control(Con, n=5), neuropathic pain plus 2 mg/kg ZP injection at GB30 and ST36(ZP-A, n=5), 10 mg/kg ZP(ZP-B, n=5), 20 mg/kg ZP(ZP-C, n=5) and 0.45 mg/kg Tramadol(Tra, n=5). Three days after the surgery, injections were administered once a day for 17 days. Withdrawal response of neuropathic rats' legs were measured by stimulating the paw of Right leg with von frey filament, acetone and radient heat on day 3, 7, 11, 15, 19 after surgery. After all treatments were completed, c-Fos in the midbrain central gray and TRPV1 & TRPA1 of DRG(L5) were analyzed. Results : Groups ZP-B and ZP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia, thermal hyperalgesia and cold allodynia compared to the control group(p<0.05, p<0.01, p<0.001). Groups ZP-B and ZP-C showed a meaningful decrease in the expression of c-fos and TRPV1 protein level compared to the control group(p<0.05, p<0.01, p<0.001). Conclusions : These results suggest that Zingiberis Rhizoma Pharmacopuncture at GB30 and ST36 could decrease mechanical & cold allodynia and thermal hyperalgesia by block the TRPV1 on the model of neuropathic pain.

• Journal of Trauma and Injury
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• 제34권2호
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• pp.81-86
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• 2021

Purpose: This study investigated the clinical outcomes of trauma patients with blunt thoracic aortic injuries at a single institution. Methods: During the study period, 9,501 patients with traumatic aortic injuries presented to Trauma Center of Gil Medical Center. Among them, 1,594 patients had severe trauma, with an Injury Severity Score (ISS) of >15. Demographics, physiological data, injury mechanism, hemodynamic parameters associated with the thoracic injury according to chest computed tomography (CT) findings, the timing of the intervention, and clinical outcomes were reviewed. Results: Twenty-eight patients had blunt aortic injuries (75% male, mean age, 45.9±16.3 years). The majority (82.1%, n=23/28) of these patients were involved in traffic accidents. The median ISS was 35.0 (interquartile range 21.0-41.0). The injuries were found in the ascending aorta (n=1, 3.6%) aortic arch (n=8, 28.6%) aortic isthmus (n=18, 64.3%), and descending aorta (n=1, 3.6%). The severity of aortic injuries on chest CT was categorized as intramural hematoma (n=1, 3.6%), dissection (n=3, 10.7%), transection (n=9, 32.2%), pseudoaneurysm (n=12, 42.8%), and rupture (n=3, 10.7%). Endovascular repair was performed in 71.4% of patients (45% within 24 hours), and two patients received surgical management. The mortality rate was 25% (n=7). Conclusions: Traumatic thoracic aortic injuries are life-threatening. In our experience, however, if there is no rupture and extravasation from an aortic injury, resuscitation and stabilization of vital signs are more important than an intervention for an aortic injury in patients with multiple traumas. Further study is required to optimize the timing of the intervention and explore management strategies for blunt thoracic aortic injuries in severe trauma patients needing resuscitation.