• 한국균학회지
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• 제25권1호통권80호
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• pp.68-76
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• 1997

섬유소자원의 이용을 목적으로 담자균류인 T. trogii를 실험 균주로 하여 합성배지에서 cellulase 생성 최적 배양조건을 검토해 본 결과는 다음과 같다. 합성배지에서 T. trogii에 의한 cellulase 생산은 $30{\sim}35^{\circ}C,\;pH\;4.0{\sim}6.0,\;and\;11{\sim}15$일 배양시 가장 높았다. 탄소원은 Avicelase와 ${\beta}-glucosidase$의 경우 CMC를, CMCase는 cellulose를 탄소원으로 했을 때 최고의 생산성을 보였으며, CMC의 최적 농도는 세 효소 모두 3%이었다. 질소원은 ammonium tartrate 첨가시 cellulase 생산이 높았으며, 그 최적 농도로 Avicelase와 ${\beta}-glucosidase$는 0.03N, CMCase는 0.04 N이었다.

• Journal of the Korean Wood Science and Technology
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• 제29권4호
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• pp.60-66
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• 2001

아세틸화 목재를 부후균에 노출시켜 laccase의 생성을 액체배양기와 고체배양기상에서 검토하고 $^{13}C$-CP/MAS NMR 분석을 통하여 목재 내 화학성분의 변화와 치환 도입된 아세틸기의 변화를 검토하여 보았다. 액체배양기상에서 laccase의 활성은 glucose를 탄소원으로 할 때 보다 높게 나타났으며, 고체배양기상에서는 laccase 의 활성이 원활히 이루어지지 않았다. $^{13}C$-CP/MAS NMR의 분석 결과 리그닌 뿐만 아니라 셀룰로오스부분에서도 아세틸화가 이루어 졌음을 알 수 있었다. 부후된 아세틸화 목재의 분석 결과, 아세틸기의 목재내 결합이 목재 부후균 T. versicolor에 대해서 안정적이었다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.155-160
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• 2017

The dye decolorization rate in a cell-free culture broth of the white-rot fungus Trametes versicolor CBR43 was studied, including the effects of inhibitors of NaCl, Zn(II), and Cd(II) on dye decolorization activity. The maximum rates of dye decolorization in cell-free culture broth were 1,410, 44.7, 41.2, and $0.19{\mu}mol{\cdot}l^{-1}{\cdot}min^{-1}$ for Acid Blue 62, Acid Black 175, Reactive Blue 4, and Acid Red 114, respectively. The inhibition effects of NaCl, Zn(II), and Cd(II) on dye decolorization were quantitatively compared using the half maximal inhibition concentration ($IC_{50}$), which indicates the concentration of an inhibitor required for 50% inhibition. Based on $IC_{50}$ values, dye decolorization in the cell-free culture broth of CBR43 was most potently inhibited by Cd(II), whereas the inhibitory effect of NaCl was relatively low. The dye decolorization rates and $IC_{50}$ data can be used in the design and development of a dye-wastewater treatment process using T. versicolor CBR43 and its operating factors.

• Journal of Microbiology
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• 제40권3호
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• pp.245-247
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• 2002

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

• 한국균학회지
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• 제40권4호
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• pp.292-295
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• 2012

구름버섯은 온대지역에서 흔히 발견되는 목재 부후균으로서 중요한 약용버섯의 일종이다. 본 연구에서는 커피박, 과립커피 및 과립 디카페인 커피의 구름버섯 균사체 생장에 미치는 영향을 조사하였다. 그 결과, 커피박은 10% 농도에서까지 그리고 과립커피의 경우 1% 미만에서 균사체 생육을 촉진시키는 것으로 확인되었다. 1% 커피박의 경우 무처리에 비해 10배 정도의 건조중량 증가가 측정됨으로써 구름균사체의 생육촉진제로서의 커피 효능을 확인할 수 있었다. 또한 커피는 균사체의 페놀화합물 및 항산화 효능 증대를 위한 유용 물질로 판단되었다.

• Journal of Microbiology
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• 제43권6호
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• pp.555-560
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• 2005

Laccase is one of the ligninolytic enzymes of white rot fungus Trametes versicolor 951022, a strain first isolated in Korea. This laccase was purified 209-fold from culture fluid with a yield of $6.2\%$ using ethanol precipitation, DEAE-Sepharose, Phenyl-Sepharose, and Sephadex G-100 chromatography. T. versicolor 951022 excretes a single monomeric laccase showing a high specific activity of 91,443 U/mg for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as a substrate. The enzyme has a molecular mass of approximately 97 kDa as determined by SDS-PAGE, which is larger than those of other laccases reported. It exhibits high enzyme activity over broad pH and temperature ranges with optimum activity at pH 3.0 and a temperature of $50^{\circ}C$. The $K_m$ value of the enzyme for substrate ABTS is $12.8{\mu}M$ and its corresponding $V_{max}$ value is 8125.4 U/mg. The specific activity and substrate affinity of this laccase are higher than those of other white rot fungi, therefore, it may be potentially useful for industrial purposes.

• Journal of Microbiology
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• 제45권3호
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• pp.213-218
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• 2007

Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.

• 한국미생물·생명공학회지
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• 제42권1호
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• pp.67-72
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• 2014

A new substrate, breadcrumbs, was investigated for biomass accumulation, the pH of the cultural broth, the formation of primary metabolites such as the proteins and endopolysaccharides of Schizophyllum commune 1768 and Trametes versicolor 353, as well as its bioconversion efficiency. The results showed that S. commune gives more mycelial mass ($23.96{\pm}0.8g/l$) and in a shorter period (4 days) than T. versicolor ($15.76{\pm}0.5g/l$ in 5 days). The pH values changed from the initial 6.1 to 3.6 in S. commune cultural broth and to 4.4 in T. versicolor cultural broth. Maximal endopolysaccharide content in the mycelia of S. commune and T. versicolor were 7.13% and 6.42%, correspondingly. Crude protein content in S. commune mycelium was 18.83 % on the 4th day of cultivation, and 20.03%, in the mycelium of T. versicolor, on the 6th day of cultivation. Kinetic parameters for the quantitative estimation of cultivation efficiency were calculated for biomass, endopolysaccharide, and crude protein concentrations.

• Environmental Engineering Research
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• 제24권1호
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• pp.144-149
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• 2019

Landfill leachate constitutes one of the most polluting wastewaters. Their treatment was considered difficult due to the presence of high concentration of organic matter, ammonia, toxic organic compounds and heavy metals. Biological processes were found to be effective in several cases, but they are limited by the presence of inhibitory compounds in leachate. In this study we develop a biological process for the leachate biodetoxification using Trametes trogii (T. trogii; CLBE55). Results show that laccase activity, mycelia growth and chemical oxygen demand (COD) removal efficiencies varied depending on the leachate and ammonium concentration. Indeed T. trogii was able to grow in the presence of low concentration of landfill leachate of 10 and 30%. In fact, the biomass produced was 4.7 and 3.7 g/L, respectively leading to a COD removal of 66 and 53%, respectively. However, when the concentration of the introduced leachate exceeds 30%, the treatment efficiency and particularly the COD removal decreases to reach 15% at 100% leachate. The effect of the ammonia was also studied and results showed that the addition of 5 g/L of ammonia inhibited totally the production of laccase and the COD removal.

• Mycobiology
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• 제47권2호
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• pp.217-229
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• 2019

Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10-30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were $50^{\circ}C$ and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.