• Mycobiology
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• 제48권2호
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• pp.104-114
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• 2020

The carbohydrate-active enzyme (CAZyme) genes of Trametes contribute to polysaccharide degradation. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters (BGCs) of Trametes remain unclear. Here, we conducted comparative analysis, detected the CAZyme genes, and predicted the BGCs for nine Trametes strains. Among the 82,053 homologous clusters obtained for Trametes, we identified 8518 core genes, 60,441 accessory genes, and 13,094 specific genes. A large proportion of CAZyme genes were cataloged into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes were divided into six strategies, and the nine Trametes strains harbored 47.78 BGCs on average. Our study revealed that Trametes exhibits an open pan-genome structure. These findings provide insights into the genetic diversity and explored the synthetic biology of secondary metabolite production for Trametes.

• 한국균학회지
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• 제33권1호
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• pp.1-10
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• 2005

본 연구는 강원도 지역에서 채집한, 구름버섯균으로 예상되는 19종의 실험균주의 외부형태와 내부구조의 특징을 파악하여 동정하고, 구름버섯균(Trametes versicolor)의 형태적 특징과 rDNA상의 ITS염기서열간의 상관관계 유무를 확인하기 위하여 Trametes versicolor와 동일 속(Genus)에 속하며 계통학적 유연관계가 가까운 Trametes villosa KCTC06866, Trametes suaveolens KCTC 26205, Trametes hirusta KCTC26200, Trametes versicolor KCTC16781 및 KCTC26203의 4종-5균주와 함께 rDNA의 Internal Transcribed Spacers(ITS1 과 ITS2)영역의 염기서열을 비교 분석하였다. 분석결과, 채집된 실험균주는 Trametes versicolor KCTC16781 및 KCTC26203과의 염기차이가 $0{\sim}6$개로 매우 유사한 근연한 양상을 나타내었고 계통도를 분석한 결과, 구름버섯으로 예상된 실험균주들과 Trametes versicolor 종들이 단계통군을 이루어 실험균주는 모두 동일종으로 확인되었으며, Trametes hirusta KCTC26200, Trametes suaveolens KCTC26205 그리고 Trametes villosa KCTC06866는 측계통을 이루었다. 채집된 19종의 균주는 외형상 다양한 채색과 무늬를 갖고 있으나 ITS1과 ITS2 영역의 염기서열을 분석한 계통수에서는 Trametes versicolor간의 색깔이나 무늬는 ITS1 및 ITS2의 염기서열과는 직접적인 유연관계가 없었다.

• 한국균학회지
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• 제49권4호
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• pp.433-440
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• 2021

The fungal genus Trametes is globally distributed and comprises various wood-decay species, including the well-known medicinal mushroom Trametes versicolor, a popular remedy in traditional Asian medicine. Trametes species produce antioxidants, which have a wide range of health benefits. The pressent study evaluated seven indigenous Trametes species from Korea, which were cultivated in three different media (dextrose-yeast extract, DY; malt extract-yeast extract, MY; malt extract broth, MEB) and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. We found that the medium consumption rate did not significantly differ between the media and among the strains (72-76%). However, the T. versicolor strains had a relatively low consumption rate (14-65%). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) tests demonstrated that culture filtrates of T. cf. junipericola, T. orientalis, T. suaveolens, and T. versicolor possessed antioxidant activity against damage from free radicals. In particular, T. cf. junipericola (DY) and T. versicolor (MY) had >80% activity in the DPPH and ABTS assays, compared with that of the positive control (ascorbic acid). Thus, our study identified promising candidates with substantial antioxidant activity among the indigenous strains of Trametes spp. from Korea.

• 한국버섯학회지
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• 제9권1호
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• pp.27-33
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• 2011

보존중인 구름버섯속 균주를 선발하여 배양 및 형태적 특성을 조사하여 비슷한 균주별로 그룹화하여 rDNA의 ITS 영역을 증폭하여 염기서열을 결정한 결과 보존시 균주의 학명과 많은 차이를 보였으며, 균사의 모양 및 색깔에도 많은 차이를 보였다. rDNA의 ITS 영역의 염기서열을 바탕으로 보존 당시 동정된 결과와 염기서열 분석을 통한 결과를 비교한 결과 종이 다른 균주가 5균주와 학명이 다른 균주가 6균주로 전체의 61%를 차지하였다. 국내에서 수집한 구름버섯의 경우 T. versicolor, T. elegans, T. gibbosa 등 3개 속으로만 동정되었고, 미국에서 수집한 균주는 T. junipericola로 동정되었다. Trametes spp의 RAPD 분석을 통한 유전적인 다형성 조사에서 T. versicolor와 T. gibbosa는 아주 다른 밴드패턴을 보였다. 또한 같은 종내에서서 분포지역에 따라 상이한 밴드 패턴을 보였다. 유전적인 유연관계 분석에서는 T. vericolor 등 4개의 분류군으로 나누어졌으며, ITS부위 유전자수준의 상동성 비교에서도 비슷한 경향을 보였다. 따라서 기존 목록과 완전히 다른 속으로 동정된 균주들에 대해서는 계통분류학적인 유연관계 분석과 보존중인 자실체 유전자와의 상동성을 비교하여 보존진균의 오염 여부를 판단하여 기존 목록의 학명을 재분류해야 할 것으로 판단된다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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• pp.132-133
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• 2007

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.500-508
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• 1999

Several white-rot fungi collected from the mountains of Korea were evaluated for their ability to decolorize azo, polymeric, and reactive dyes. Strains CJ-105, CJ-212 and CJ-315, identified as Trametes sp., Pleurotus sp. and Fomes sp., respectively, showed higher potential for decolorization of those dyes in either solid or liquid media. For Trametes sp. CJ-105, 100ppm of Remazol Brilliant blue R and 500ppm of Acid Red 264 were completely decolorized after 2 days under liquid culture. The dominating ligninolytic enzyme existing in the culture broth was laccase (E.C. 1.10.3.2). Also, Pleurotus sp. CJ-212 and Fomes sp. CJ-315 showed similar patterns in decolorization of Remazol Brilliant Blue R and Acid Red 264. The extent of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic enzymes produced by each strain. In addition to that Trametes sp. CJ-105 was highly effective in degradation of polycyclic aromatic hydrocarbons and pentachlorophenol by the activity of the ligninolytic enzymes produced. In this study, we found that white-rot fungi, Trametes sp. CJ-105(KFCC 10941), Pleurotus sp. CJ-212(KFCC 10943) and Fomes sp. CJ-315(KFCC 10942), were effective in decolorizing a wide range of structurally different synthetic dyes, as well as some chemical compounds which are known to be hardly degradable.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.477-483
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• 1999

For Trametes sp. CJ-105, a kind of white-rot fungi which was collected from the mountain of Korea and was proven to be effective in decolorizing a wide range of structurally different synthetic dyes, the optimum conditions for mycelial growth and laccase(E.C. 1.10.3.2) production were investigated. Among various carbon sources, glucose showed the highest potential for the mycelial growth and laccase production, the optimum concentration being 2% glucose. For the nitrogen source, asparagine was good for the mycelial growth, while ammonium tartrate for laccase production(optimum concentration: 0.04%). The addition of thiamine and biotin increased both th emycelial growth and laccase production. When 2,5-xylidine was added as an inducer after the first day of culture, the production of alccase was seven-times higher than that in the absence of the inducer. The optimum pH and temperature conditions for laccase production by Trametes sp. CJ-105 were pH 5.0 and $25^{\circ}C$, respectively. In the 5L fermentation, the production of laccase reached a maximum of 340U/ml at the time when the ammonium ion was being rapidly depleted.

• 한국균학회지
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• 제48권1호
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• pp.1-13
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• 2020

버섯은 예로부터 암과 염증 질환의 약재로써 많이 사용되어왔다. Trametes cubensis 버섯종은 현재까지 많은 연구가 이루어지지 않았고, 형태학적 특성만 알려져 있고 효능에 관한 연구 보고가 미흡한 실정이다. 따라서 본 연구에서는 T. cubensis 균사체 추출물(Trametes cubensis extract, TCE)의 혈관생리학적 효능을 알아보기 위해 세포와 분자수준에서의 연구를 수행하였다. 먼저 TCE를 처리하였을 때, 세포 독성은 없었고 세포성장을 촉진시켰다. 또한 세포이동이 TCE에 의해 증가하는 것을 확인하였다. 다음으로 LPS (Lipopolysaccharide)에 의해 유도된 THP-1 세포의 내피세포 부착이 TCE에 의해 억제되는 것을 확인하였다. 또한 세포신호전달 경로 분석을 한 결과, TCE에 의해 활성산소가 증가하였으며, Akt억제를 통하여 p38 MAPK가 활성화되었다. 그리고 TCE가 촉발하는 세포성장, 세포이동, 단핵구 부착 등은 p38 MAPK (mitogen-activated protein kinase)에 의해 조절되었으며, 활성산소와는 관련이 없었다. 결론적으로, TCE는 세포성장, 세포이동, 단핵구 부착을 조절하였으며, 이는 TCE가 동맥경화와 같은 심혈관계 질환의 예방 및 치료제 혹은 혈관기능개선제로 개발될 가능성이 있음을 암시한다.

• Mycobiology
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• 제51권4호
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• pp.256-263
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• 2023

Species in the genus Trametes (Basidiomycota, Polyporales) have been used in natural medicine for a long time. Many studies reported that mycelia or fruiting bodies of Trametes spp. exhibited effects of antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. However, comparative analysis in this genus is scarce due to limitation of morphological identification and the sample number. In this study, the 19 strains of seven Trametes species were chosen to generate a five-gene-based phylogeny with the 31 global references. In addition, 39 culture extracts were prepared for 13 strains to test for anticancer and antibacterial activities. Strong anticancer activities were found in several extracts from T. hirsuta and T. suaveolens. Anticancer activities of T. suaveolens, T. cf. junipericola and T. trogii were first described here. The antibacterial ability of T. versicolor and T. hirsuta extracts has been confirmed. The antibacterial activities of T. suaveolens have been reported at the first time in this study. These results suggest an efficient application of the genus Trametes as the drug resources especially for anticancer agents.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.630-635
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• 1997

Decolorization of congo red, methyl orange, poly R478, remazol brilliant blue R and crystal violet by white-rot fungus Trametes sp. CJ-105, isolated in Korea, was investigated. Remazol blue and methyl orange were almost completely decolorized after 2 days of culture, but congo red, crystal violet and poly R478 were decolorized by about 80%, 40% and 30% after 10 days of culture, respectively. As a result of determination of cell mass and enzyme activity, it was shown that color removal efficiency was related to cell mass and enzyme activity, and also found that only laccase (E.C.1.10.3.2) activity was existed in the culture broth. The decolorization ratios of remazol blue in the concentrations of 100ppm to 3, 000 ppm were 85% and above after 2 days of culture. In this study, we found that white-rot fungus, Trametes sp. CJ-105, was effective in decolorizing a wide range of structurally different synthetic dyes.