• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.158-163
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• 1999

In order to find less toxic antiherpetic agents, antiviral activities of quercitrin against two strains of pathogenic viruses such as herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) were determined in Vero cells using plaque reduction assay in vitro. Quercitrin showed a concentration-dependent decrease in plaque formation of HSV-1 and HSV-2. It also exhibited more potent antiherpetic activity on HSV-1 with 50% effective concentration (EC$_{50}$) of 20.4 $\mu$g/ml than on HSV-2 with EC$_{50}$ of 30.4 $\mu$g/ml. The combined antiherpetic effects of quercitrin with nucleoside antiherpetic agents, acyclovir and vidarabine, were examined on the multiplication of these two strains of herpesviruses in Vero cells by the combination assay. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combinations of quercitrin with acyclovir and vidarabine on HSV-1 showed more potent synergism with CI values of 0.27-0.81 for 50%, 70%, 90% effective levels than those on HSV-2 with CI values of 1.03~2.20..20.

• Korean Journal of Pharmacognosy
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• v.30 no.3
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• pp.244-249
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• 1999

In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.

• Journal of the Optical Society of Korea
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• v.18 no.2
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• pp.174-179
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• 2014

Since in vitro neural recording and imaging applications based on a surface plasmon resonance (SPR) technique have expanded dramatically in recent years, cytotoxicity assessment to ensure the biosafety and biocompatibility for those applications is crucial. Here, we report the cytotoxicity of the SPR substrate incorporating a flint glass whose refractive index is larger than that of a conventional crown glass. A high refractive index glass substrate is essential in neural signal detection due to the advantages such as high sensitivity and wide dynamic range. From experimental data using primary hippocampal neurons, it is found that a lead-based flint glass is not appropriate as a neural recording template although the neuron cells are not directly attached to the toxic glass. We also demonstrate that the adhesion layer between the glass substrate and the gold film plays an important role in achieving the substrate stability and the cell viability.

• Macromolecular Research
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• v.17 no.6
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• pp.411-416
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• 2009

The color stabilization of antimicrobial blends was studied by using poly(hexamethylene guanidine) phosphate (PHMG) as a highly efficient biocidal and nontoxic agent. The Taguchi method was used to determine the optimum conditions for the blending of PHMG in polypropylene (PP) matrix. To improve the yellowing phenomena, two kinds of stabilizer were used together: tetrakis[methylene(3,5-di-t-butyl-4-hydroxyhydrocinnamate)](IN1010) from phenol and tris(2,4-di-t-butylphenylphosphite) (IF168) from phosphorus. According to blend composition and mixing condition, six factors were chosen, with five levels being set for each factor. The orthogonal array was selected as the most suitable for fabricating the experimental design, L25, with 6 columns and 25 variations. The-smaller-the-better was used as an optimization criterion. The optimum conditions for these parameters were 10 phr for PHMG, 2 phr for IN1010, 1 phr for IF168, 10 min for mixing time, $210^{\circ}C$ for mixing temperature, and 30 rpm for rotation speed. Under these conditions, the yellowness index of the blend was 1.52. The processibility of the blends was investigated by Advanced Rheometric Expansion System (ARES). The blend with 0.5 w% PHMG content, diluted with PP, exhibited an antimicrobial characteristic in the shake flask method.

• The Korean Journal of Food And Nutrition
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• v.21 no.2
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• pp.256-262
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• 2008

This study was carried out to compare the hair mineral status of obese, over-weighted and non-obese individuals, to gather basic data for customizing menu development and to create an education manual for the obese persons. Food preferences or various disease states could be suggested by different mineral patterns in TMA(tissue mineral analysis). The results indicated that Zn status was considerably lower in the obese individuals than in the non-obese(p<0.001) whereas hair Na(p<0.0001), K(p<0.01) and Fe(p<0.05) were at significantly higher levels in the obese individuals. The ratio of Ca/K(p<0.001) was significantly lower in the obese than in the non-obese. But the levels of hair toxic minerals such as Sb, As, Hb, Al et al. were not differ according to BMI groups. The obtained data demonstrate the changes of hair mineral content in both overweight and obese individuals thus suggesting metabolic mineral disturbance in those groups.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.4
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• pp.898-906
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• 2007

This study was to evaluate the effects of oral administration of Processed Sulphur on Hepatotoxicity. Processed Sulphur was administered orally to rats for 28 days. We measured the body and liver weight index, heamtological and biomedical parameters. We also observed the histopathological changes of liver in rats. No significant differences in body weight, liver weight index, heamtological and biomedical parameters and histopathological changes of hepatocyte between control and Processed Sulphur fed group were found. Our data indicate that hepatotoxicity was not caused by oral medication of Processed Sulphur up to 60mg/200g/day for 28 days in rats. Therefore, Processed Sulphur appears to be safe and non-toxic in these studies and a no-observed adverse effect level (NOAEL) in rats is established at 60mg/200g/day. The data could provide satisfactory preclinical evidence of safety to launch clinical trial on standardized formulation of mineral extracts.

• Geomechanics and Engineering
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• v.1 no.4
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• pp.291-306
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• 2009

One-dimensional soil-column studies were carried out to understand the interaction of three industrial effluents namely amino acid ('highly acidic'), surfactant ('highly organic') and pharmaceutical ('organic and toxic') on the physicochemical behavior, index properties and shear strength of bentonite due to artificial contamination extending to nearly 300 days. Changes in inorganic and organic pollutants present in the effluents due to the interaction of the above effluents and soil were assessed to understand the physico-chemical behaviour. Batch and continuous modes of operation, 8 hrs and 16 hrs Hydraulic Retention Time [HRT] and 25%, 50% concentrations of effluents, were the parameters considered. Amino acid, surfactant and pharmaceutical effluents have shown a high variation in pH (7 to 8) after artificial contamination on bentonite that is their original characteristics of the above effluents have been completely reversed. Further, it is found that the shear strength of bentonite has reduced by about 20%, and with respect to liquid limit and plastic limit shows an increasing trend with time within the period of contamination.

• The Journal of Korean Medicine
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• v.21 no.3
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• pp.213-219
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• 2000

We treated a 53 year-old male patient. He had alcoholic liver cirrhosis and diabetes. The main symptoms were fatigue, ascites, edema, anorexia, indigestion, constipation, and abdominal pain. We diagnosed him as 'the retention of pathogenic damp-heat in the liver and gall bladder’ and adminstered Saenggangeonbi-tang three times a day. We observed clinical symptoms, liver function test(AST, ALT, ${\gamma}-GT$, ALP, total bilirubin), nutritional state(total protein, albumin) and capillary blood glucose. After treatment, all the main symptoms disappeared and the patient's nutritional state has improved. Child's grade using the improvement index in liver cirrhosis has changed from B to A. Although the very mechanism of Saenggangeonbi-tang was not proven, we could suggest that Saenggangeonbi-tang is not toxic to the liver and has a good effect on alcoholic liver cirrhosis with diabetes mellitus.

• International Journal of Safety
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• v.9 no.1
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• pp.35-42
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• 2010

We measured the concentrations of air pollutants at several residential sites, roadside sites and industrial sites in Iwate Prefecture, Japan. And the concentration distributions of air pollutants were estimated by atmospheric dispersion model using air emissions data. Based on those results, we calculated environmental risk of air pollutants emitted in Iwate Prefecture. As a result, it was found that the surround of factories with high emissions and highly toxic chemicals and the roadsides were high risk area, benzo(a)pyrene, formaldehyde and ozone exceeded the $10^{-5}$ risk level. Moreover, we tried to use "Loss of life expectancy: LLE" for an index to explain those risk to general public intelligibly. The total LLE of the carcinogenic chemicals was about 8.6 hours. Moreover, LLE of ozone was about 9.2 hours. Ozone has a big influence compared with the carcinogenic chemicals.

• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.263-273
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• 1999

Cheung-Gan-Haeju tang has been used on 3 cases of alcoholic liver disease patients complicated with ascites, clinical symptom(fatigue. jaundice, urine dark, indigestion, anorexia. ascites etc), liver function (AST, ALT, ${\gamma}$-GT, ALP, total bilirubin), and index of nutritional state (total protein, albumin, cholesterol) were improved after the adminstration. Although the exact mechanism involved in the effects of Cheung-Gan-haeju tang on these disease is still unknown, it is possibly suspected that Cheung-Gan-Haeju tang is non-toxic to liver and has beneficial effects on treating alcoholic liver disease complicated ascites. Further reports with many case, however, will be needed.