• 제목/요약/키워드: Total phenolic compound

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Antioxidant Activities of Fractions from Sedum sarmentosum

  • Kim, Choon-Young;Lee, Min-Young;Park, In-Shik
    • Preventive Nutrition and Food Science
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    • 제11권1호
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    • pp.6-9
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    • 2006
  • This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT(synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61 % and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

자색고구마 분말 첨가량을 달리한 쿠키의 항산화 활성 및 품질 특성 (Quality Characteristics and Antioxidant Activities of Cookies added with Purple Sweet Potato Powder)

  • 유아남;정다혜;정지혜;김현숙
    • 한국식품조리과학회지
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    • 제29권3호
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    • pp.275-281
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    • 2013
  • This study was performed to evaluate the quality characteristics and antioxidative activities of cookies prepared with different amounts of purple sweet potato powder. Moisture and crude ash contents were lowest in the control group followed in order by the 10%, 20%, and 30% purple sweet potato powder addition groups. The lightness and yellowness values measured by Hunter color system decreased based on the amount of purple sweet potato powder concentration added to cookies. The total phenolic compound and anthocyanin contents were the highest in 30% purple sweet potato powder added cookies. The DPPH radical scavenging activity of 30% purple sweet potato powder added cookies showed the highest values among groups. In the sensory evaluation, the scores for taste, texture and overall preference were significantly highest in cookies with 10% and 20% purple sweet potato powder. Considering the scores of antioxidant activity and sensory evaluation, the concentration of between 10% and 20% addition of purple sweet potato powder would be ideal for development of functional cookies.

방울토마토 첨가량을 달리한 당화 방울토마토죽의 항산화 및 ACE 저해 효과 (Physiological Activities of Saccharified Cherry Tomato Gruel Containing Different Levels of Cherry Tomato Puree)

  • 김진숙;김자영;장영은
    • 한국식품조리과학회지
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    • 제28권6호
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    • pp.773-779
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    • 2012
  • This study was conducted to investigate the physiological activities of saccharified cherry tomato gruel containing different levels(0, 15, 30, and 45%) of cherry tomato puree. The total phenolic compound and flavonoids contents of saccharified cherry tomato gruel with cherry tomato puree were 1.73-5.09 mg/g and 0.28-7.01 mg/g, respectively. DPPH radical scavenging activities of saccharified cherry tomato gruel with cherry tomato puree were 8.67-92.58%, respectively. The ABTS radical scavenging activities of saccharified cherry tomato gruel with cherry tomato puree ranged from 6.02 to 61.59%. The ACE inhibitory activity of cherry tomato rice gruel increased with cherry tomato puree showed a range of 38.85-62.15%, respectively. With increasing additions of cherry tomato puree, the total phenolic compound and flavonoid contents, DPPH and ABTS radical scavenging activities and, ACE inhibitory activities were increased significantly (p<0.05). In conclusion, saccharified cherry tomato gruel could be used as a gruel processing method to in order to increase the functional values of gruels.

Preparation of Functional Food in Combination of Tartary Buckwheat and Bean Flour Mixes by Hot Melt Extrusion Process

  • Azad, Obyedul Kalam;Kang, Wie Soo;Park, Cheol Ho
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.71-71
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    • 2018
  • The aim of this study was to prepare a new functional food with enriched bioactive compound by the combination of Tartary buckwheat and bean flour mixes. Two types of bean such as: black bean and white bean with the ratio of 5, 10, 15 % were used to develope a food formulation. The solid formulation of functional food was prepared by the hot melt extrusion process (HME) at the temperature of 60, 90, $120^{\circ}C$. Total phenolic content (TPC), total flavonoid (TF) and DPPH were analysed of the prepared food by the use of spectrophotometer. Results demonstrated that TPC (254 mg/g), TF (18.22 mg/g) and DPPH (88%) were significanlty increased in the receipe of 10% black bean and 90% Tartary buckwheat mixes among the formulation at $90^{\circ}C$. As therefore, it is concluded that the optimum ratio of bean and Tartary buckwheat flour mixes should be considerd for the preparation of high quality functional food.

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Antioxidant Activities of Ginseng Seeds Treated by Autoclaving

  • Bae, Hye-Min;Kim, Sung-Soo;Cho, Chang-Won;Yang, Deok-Chun;Ko, Sung-Kwon;Kim, Kyung-Tack
    • Journal of Ginseng Research
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    • 제36권4호
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    • pp.411-417
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    • 2012
  • Ginseng seeds were treated with different autoclaving temperatures and autoclaving times, and extracted with 80% methanol to measure changes in antioxidant activity. The antioxidant activity of ginseng seeds treated by autoclaving was measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2'-aziono-bis(3-ethylbenzthiazoline)-6-sulfonic acid radical scavenging activity, superoxide dismutase SOD-like activity, ferric reducing antioxidant power (FRAP), and total phenolic compound content. As autoclaving temperature and time were increased, the L lightness value decreased and the redness value tended to increase. Total phenolic compound content was about three times higher in ginseng seeds treated with autoclaving at 130°C than in ginseng seeds that were not treated. DPPH radical scavenging activity and ABTS radical scavenging activity increased as autoclaving temperature and time were increased. In particular, when the concentration was 100 ppm, the ABTS radical scavenging activity was 91.80% in ginseng seeds treated by autoclaving at $130^{\circ}C$, which was the highest antioxidant activity. FRAP and SOD-like antioxidant activity tended to increase significantly as autoclaving temperature and time were increased.

n-Hexane 및 Ethyl Acetate를 이용한 Willamette 품종 라즈베리추출물의 페놀 화합물 함량 및 항산화 효능 연구 (Studies on the Phenolic Compound Content and Antioxidant Efficacy of Rubus idaeus (Raspberry) Cultivar 'Willamette' Fruit Extract Using n-Hexane and Ethyl Acetate)

  • 박규근;윤여필
    • 대한화장품학회지
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    • 제46권4호
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    • pp.329-337
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    • 2020
  • 본 연구는 willamette 품종의 동결건조라즈베리분말, n-hexane 및 ethyl acetate를 이용하여 라즈베리 추출물(Rubus idaeus (Raspberry) fruit extract, RIFE)을 제조한 다음 페놀 화합물 함량, 철 이온 환원능력, 그리고 라디칼 소거 능력의 측정을 수행하였다. Willamette 품종 라즈베리추출물은 10% 농도까지 세포독성을 보이지 않았다. 해당 농도로 실험을 수행한 결과 총 페놀 화합물 함량은 375.3 ppm, 총 플라보노이드 함량은 43.46 ppm이 포함된 것을 확인하였고, ferric reducing antioxidant power (FRAP) reagent를 이용한 철 이온 환원능력은 FeSO4 0.532 mM에 해당하는 환원능력을 가졌음을 확인하였다. 이를 바탕으로 항산화 소재의 대표적 원료인 L-ascorbic acid와 비교하는 라디칼 소거능 실험을 실시하였다. 그 결과 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 라디칼 소거능력은 94.5 ± 0.7%를 나타냈고, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) 라디칼 소거능은 99.4 ± 2.82%를 나타냈으며, nitric oxide (NO) 라디칼소거능은 88.5 ± 0.4%를 나타냈다. 표준인 L-ascorbic acid 용액과 비교할 때 25 - 50 ppm 사이의 효능을 지닌 DPPH 라디칼 소거능 / 100 ppm에 근접한 효능을 지닌 ABTS 라디칼 소거능 / 1,000 ppm 이상의 효능을 지닌 NO 라디칼 소거능을 가졌음을 확인하였다. 이러한 결과들은 willamette 품종 라즈베리추출물이 항산화 활성을 가지는 효과적인 화장품 소재로 적용 가능하다는 것을 시사한다.

볶음조건과 저온압착이 아마씨유(Linum usitatissimum L.)의 지방산 조성 및 휘발성 성분에 미치는 영향 (Effect of Roasting Condition and Cold-pressed Flaxseed (Linum usitatissimum L.) oil on Fatty Acid Composition and Volatile Compound)

  • 원새봄
    • 융합정보논문지
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    • 제10권11호
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    • pp.177-184
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    • 2020
  • 본 연구는 불포화지방산 중 α-리놀렌산이 풍부한 아마씨에서 볶음조건과 저온압착법으로 착유된 아마씨유의 화학적 변화에 미치는 영향을 조사하고자 하였다. 볶지 않은 아마씨와 볶은 아마씨(150℃와 200℃에서 10분과 20분)를 가정용 저온압착기를 사용하여 저온압착으로 착유하여 착유수율, 총 페놀 함량, 지방산 조성 및 휘발성 성분을 분석하였다. 착유수율은 볶지 않은 아마씨에서보다 볶은 아마씨에서 높게 나타났다. 총 페놀 함량은 150℃에서 20분 동안 볶아서 착유한 경우 다른 착유군보다 유의적으로 높은 반면, 200℃에서 착유한 경우는 볶지 않은 생 아마씨유보다 낮았다. 지방산의 조성은 볶음조건에 의해서 차이를 보이지 않았다. 휘발성 성분은 총 함량과 지방산패의 지표로 알려진 aldehyde, ketone, furan은 볶음온도와 시간이 증가됨에 따라서 증가되었고, Maillard 반응에 영향을 주는 pyrazine도 200℃에서 볶아서 착유한 아마씨유에서 증가하였다. 결과적으로 가정용 저온압착기로 착유된 아마씨유는 볶지 않은 아마씨보다는 150℃에서 20분 동안 볶은 후 저온압착으로 착유하는 방법이 안전한 추출과정으로 제안된다.

발아에 따른 팥의 유리형 및 결합형 기능성분 변화 (Changes in Free and Bound Forms of Bioactive Compound Profiles of Adzuki Bean with Germination)

  • 김민영;장귀영;오남석;백소윤;김길호;김경미;김홍식;이준수;정헌상
    • 한국식품영양과학회지
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    • 제46권8호
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    • pp.937-943
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    • 2017
  • 본 연구에서는 발아에 의한 팥의 기능성분 변화를 살펴보기 위하여 6일 동안 발아시키면서 유리형 및 결합형 폴리페놀, 플라보노이드, 페놀산 및 안토시아닌의 변화를 분석하였다. 유리형 폴리페놀 및 플라보노이드 함량은 발아기간이 증가함에 따라 각각 약 2.6배 및 5배 정도 증가하였으며, 결합형 폴리페놀 및 플라보노이드 함량은 발아 초기 감소하였다가 발아 3일 및 2일 이후 각각 증가하였다. 발아기간에 따른 구성 페놀산의 변화의 경우 ferulic acid, veratric acid, hesperidin, salicylic acid, naringenin 및 hesperidin과 총 페놀산은 유리형 및 결합형 페놀산 함량이 모두 증가하였지만, 안토시아닌 및 안토시아니딘은 침지 및 발아과정에서 손실되어 감소하였다. 본 연구 결과 팥을 발아시킬 경우 폴리페놀, 플라보노이드 및 페놀산 함량이 증가함에 따라 팥에 함유된 기능성분의 이용성을 증대시키기 위해서는 발아공정의 적용이 효과적이라고 판단된다.

우엉차의 껍질 제거와 로스팅에 따른 추출온도별 항산화 효과 (Effects of Roasting and Peeling Process and Extraction Temperature on the Antioxidant Activity of Burdock Tea)

  • 박미영;우등광;박영현
    • 한국약용작물학회지
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    • 제24권5호
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    • pp.351-359
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    • 2016
  • Background: We investigated the optimal aqueous extraction conditions for recovery of high yields of total phenolic compounds from roots of Arctium lappa L. (burdock, Asteraceae), and we compared their antioxidant capacity. Methods and Results: The antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, and oxygen radical absorbance capacity assays. In addition, the major phenolic compounds present in the extracts were determined by high performance liquid chromatography analysis. Our results suggest that the roasted burdock $100^{\circ}C$, 15 min extract exhibited the strongest radical scavenging activity and possessed the highest concentration of phenolic compounds. The polyphenol content of both dried burdock and roasted burdock significantly increased with increase in the extraction temperature and time. Conclusions: These results indicated a relationship between phenolic compound levels in burdock and their free radical scavenging activities. This suggests that phenolic compounds significantly increase the antioxidant potential of burdock extracts.

Bioremediation of Phenolic Compounds Having Endocrine-disrupting Activity Using Ozone Oxidation and Activated Sludge Treatment

  • Nakamura, Yoshitoshi;Daidai, Masakazu;Kobayashi, Fumihisa
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제9권3호
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    • pp.151-155
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    • 2004
  • The bioremediation of water system contaminated with phenolic compounds having endocrine-disrupting activity, i.e. 2,4-dichlorophenol, 2,4-dichlorophenoxy acetic acid (2,4-D), and 2,4,5-trichlorophenoxy acetic acid (2,4,5-T), was investigated by using ozone oxidation and activated sludge treatment. Ozone oxidation (ozonation time: 30 min) followed by activated sludge treatment (incubation time: 5 days) was an efficient treatment method for the conversion of phenolic compounds in water into carbon dioxide and decreased the value of total organic carbon (TOC) up to about 10% of initial value. Furthermore, 2,4-D was dissolved in water containing salt, i.e. artificial seawater (ASW), and this water was used as model coastal water contaminated with phenolic compounds. The activated sludge treatment (incubation time: 5 days) could consume significantly organic acids produced from 2,4-D in the model costal water by the ozone oxidation (ozonation time: 30min) and decrease the value of TOC up to about 35% of initial value.