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Growth and Amino Acid Contents of Spirulina platensis with Different Nitrogen Sources

  • Park, Aeran;Kim, Song-Gun;Yoon, Byung-Dae;Oh, Hee-Mock
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.6
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    • pp.368-372
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    • 2003
  • The growth and amino acid contents of the cyanobacterium, Spirulina platensis strain NIES 46, were investigated using ammonium, nitrate, nitrite, or urea as the sole nitrogen source in a batch culture. Chlorophyll a concentration was highest at 2,096$\mu\textrm{g}$/L in the nitrate group after 10days of cultivation, while the dry weight of S. platensis was highest at 4.5g/L in the ammonium group after 30days of cultivation. The total amino acid content was highest at 174mg/g dry weight of S. platensis in the urea group at the end of the cultivation period, yet the amino acid patterns for S. platensis were similar for all the experimental groups. Therefore, it seemed that the growth and amino acid composition of S. platensis varied depending on the type of nitrogen sources, while the amino acid patterns were not changed. Also, the most efficient harvesting time for S. platensis seemed to be approximately 10 days after cultivation.

Carbohydrate Concentration and Composition in Source and Sink Tissues of Two Tall Fescue Genotypes

  • Song, Beom-Heon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.4
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    • pp.273-278
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    • 1998
  • Carbohydrate metabolism and partitioning are dependent on relationships between sources and sinks which can be affected by rates of photosynthesis and respiration. Fructan, the major form of stored carbohydrate in tall fescue (festuca arundineacea Schreb.), changes in concentration during growth and in response to the environment. Objectives of this study were i) to examine the content and the composition of carbohydrates in five tissues (mature leaf blade, immature leaf blade, leaf elongation zone, terminal meristem, and root tips) of two tall fescue genotypes, one with high yield per tiller (HYT) and one with low yield per tiller (LYT), and ii) to compare the reserved and utilized carbohydrates among above five different tissues, particularly between the leaf elongation zone and root tips. The established vegetative tillers of the HYT and LYT genotypes were grown in a controlled-environment growth chamber. Water-soluble carbohydrate (WSC) in the leaf elongation zone was about 22% of dry weight in the HYT and about 19% in the LYT genotype. The root tip also had high WSC, about 12% of dry weight in the HYT and 6% in the LYT genotype. Hexoses and sucrose were the major components of total WSC in all tissues except the leaf elongation zone. The growing tissues (sinks), i.e., the leaf elongation zone and root tip, had a high proportion of low degree of polymerization fructan, i.e., 3 to 8 hexose units.

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Chemical Compositions of Schizandra nigra Maxim. (흑오미자의 성분분석)

  • 현규환;김학진;신수철
    • Korean Journal of Plant Resources
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    • v.13 no.1
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    • pp.35-40
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    • 2000
  • The results from the determination of contents of components in Schizandra nigra are as follows. The contents of malic acid and citric acid in Schizandra nigra was 38,691 and 3,330 ppm/dry weight 100g. The contents of total phenolic compounds in Schizandra nigra was 1.560%. The predominating phenolic acid was cinnamic acid, gentisic acid, coumalic acid, chlorogenic acid and ferulic acid. Contents of crude lipids in Schizandra nigra was 160.5mg/g. Most of fatty acid in lipids was oleic acid, linoleic acid and linolenic acid as a unsaturated fatty acid, and palmitic acid as a saturated fatty acid. In case of essential oils, The predominating components in Schizandra nigra was caryophyllene, calarene, cubebene, acoradiene and $\beta$-himachalene.

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A Comparison of Vegetative Growth of Kentucky Bluegrass and Perennial Ryegrass Cultivars in Different Levels of Shade (그늘하에서의 Kentucky Bluegrass와 Perennial Ryegrass 품종의 생육비교)

  • 구자형;김태일;원동찬;송남현;송천영
    • Asian Journal of Turfgrass Science
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    • v.5 no.1
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    • pp.23-32
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    • 1991
  • The purpose of this study was to determine the vegetative growth of two cool season turfgrasses during summer season in Korea. Ten respective cultivars of Kentucky bluegrass [Poapratensis L.] and perennial ryegrass [Lolium perenne L.] were exposed to unshaded, 30% and 60% shade for 4odays. First mowing height was 7cm, and then clippings were harvested every ten days at the same height. Shading increased the total length of clippings of all cultivars of two turfgrasses, but fresh and dry weight were significantly decreased. The highest shoot length obtained at 30% shade in Kentucky bluegrass and at 60% shade in perennial ryegrass. 'Rugky', 'Sydsport', 'Wabash' and 'Baron' of Kentucky bluegrass and 'Repel', 'Omega II', 'Ovation', 'Linn', 'Bell' and 'Manhattan' of perennial ryegrass showed relatively high tolerance to shade. Especially, in 'Citation II' perennial ryegrass, fresh and dry weight were increased by 30% shade. The number of tillers generally decreased with increasing shade. In 30% shade, 'Midnight' Kentucky bluegrass and 'Omega II' perennial ryegrass were least reduced and 'Baron' Kentucky bluegrass and 'Pennant' perennial ryegrass were most reduced. The cultivars of lower growth habit in full sunlight showed shade tolerance compared to those of higher growth habit.

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Integrated Hydrolyzation and Fermentation of Sugar Beet Pulp to Bioethanol

  • Rezic, Tonic;Oros, Damir;Markovic, Iva;Kracher, Daniel;Ludwig, Roland;Santek, Bozidar
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1244-1252
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    • 2013
  • Sugar beet pulp is an abundant industrial waste material that holds a great potential for bioethanol production owing to its high content of cellulose, hemicelluloses, and pectin. Its structural and chemical robustness limits the yield of fermentable sugars obtained by hydrolyzation and represents the main bottleneck for bioethanol production. Physical (ultrasound and thermal) pretreatment methods were tested and combined with enzymatic hydrolysis by cellulase and pectinase to evaluate the most efficient strategy. The optimized hydrolysis process was combined with a fermentation step using a Saccharomyces cerevisiae strain for ethanol production in a single-tank bioreactor. Optimal sugar beet pulp conversion was achieved at a concentration of 60 g/l (39% of dry weight) and a bioreactor stirrer speed of 960 rpm. The maximum ethanol yield was 0.1 g ethanol/g of dry weight (0.25 g ethanol/g total sugar content), the efficiency of ethanol production was 49%, and the productivity of the bioprocess was 0.29 $g/l{\cdot}h$, respectively.

Effects of the ectomycorrhizal fungus Pisolithus tinctorius and Cd on physiological properties and Cd uptake by hybrid poplar Populus alba × glandulosa

  • Han, Sim-Hee;Kim, Du-Hyun;Lee, Jae-Cheon
    • Journal of Ecology and Environment
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    • v.34 no.4
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    • pp.393-400
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    • 2011
  • The effects of the ectomycorrhizal fungus Pisolithus tinctorius and cadmium (Cd) on physiological properties and Cd uptake by Populus alba ${\times}$ glandulosa was investigated under greenhouse conditions. Cd treatment decreased the photosynthetic rate ($P_N$) of both non-mycorrhizal (NM) plants (16.3%) and ectomycorrhizal (ECM) plants (11.5%). In addition, the reduction in total dry weight by Cd treatment was greater in ECM plants (24.3%) than that in NM plants (17.6%). Mycorrhizal infection increased the $P_N$ and transpiration rate in both control and Cd-treated plants. Cd treatment increased superoxide dismutase (SOD) activity and decreased glutathione reductase activity, and the increase of SOD activity by Cd treatment was greater in NM plants (40.3%) than that in ECM plants (3.7%). Thiol content increased in both NM and ECM plants treated with Cd solution, and the increase in thiol content in NM plants (43.9%) was greater than that of ECM plants (15.6%). Cd uptake in the leaves, stems, and roots of ECM plants was 69.9%, 167.2% and 72.8%, respectively, higher than in the NM plants. However, the increase in Cd uptake ability of ECM plants resulted in a reduction in dry weight.

Effects of Root Restriction by Clay Pot and Root Contrl Bag on Growth of Acer Palmatum (토분과 Root Control Bag에 의한 근권제한이 단풍나무의 생장에 미치는 영향)

  • 김동욱;김민수
    • Journal of the Korean Institute of Landscape Architecture
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    • v.28 no.4
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    • pp.1-8
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    • 2000
  • This study is conducted to evaluate the effects of root restriction on growth of maple(Acer palmatum Thumb.). Tow types of container such as clay pot(CP), root control bag(RCB) were used to restrict maple's root and each type of container was divided into 5 sizes. The containers with plants were buried just below the soil level and maples planted directly in the soil (nonrestricted root treatment) were included as comparison. Data were collected on dry weight of leaf, trunk, thick root, rootlet and soil water potential. We have analyzed, simple linear regression, Pearson's Correlation analysis, Duncan's multiple rang test, and Covariance Analysis using SAS statistical software. The results of analysis based on these data are as follows; 1. Total dry weight of maple in CP was significantly larger than in RCB. 2. Difference in growth reduction by the kinds of containers was induced by different hydraulic diffusion ratios between container and soil. Difference in growth reduction by the size of container was induced by the difference density of rootlet and soil moisture contents in the container. 4. Commercial products of root control of root control bag appeared not proper for countries in which fluctuation of rainfall is severe. Because maples in RCB were restricted by excess soil water in the rainy season, or by lack of soil water in the dray season.

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Effects of Black Sugar® and Mineral® Supplementation on Growth performance and Meat Quality of Hamwoo Steers in Fattening Period

  • Kim, Kwan Sik;Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.3
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    • pp.202-208
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    • 2014
  • This study was carried out investigate the effects of dietary addition of mineral and sugar on the dry matter intake, daily gain, yield grade and quality grade of Hanwoo (Bos taurus coreanae) steers. Three diets fed to steers included a control diet (concentrate mix and rice straw : C) and two treatments diet (control diet + black sugar 100 g + mineral 100 g : T1, and control diet + black sugar 150 g + mineral 50 g : T2). The results are summarized as follows; total feeding intake, body weight gain and daily gain did not show significant differences among the three treatments. Cold carcass weight was significantly (p<0.05) higher in T2 than in the other two treatments (C and T1). There was no significant difference in yield traits of back fat thickness, longissimus muscle area and yield grade among the three treatments (C, T1 and T2). Marbling score showed significantly (p<0.05) higher in order of T2 (5.67) > T1 (4.67) > C (3.67). Meat color, fat color, texture and maturity were no significant difference. Quality grade was higher in T2 than in the other two treatments (C and T1), but there was no significant difference. The results show that marbling score and quality grade of Hanwoo can be increased by high dry matter intake with feeding addition of mineral and sugar.

Studies on the Boron Metabolism of Orchid -Influences on the Cell Wall Structure and its Components- (양란의 붕소 대사에 관한 연구 -특히 세포벽 형성 및 분획 조성에 미치는 영향-)

  • 강영희
    • Journal of Plant Biology
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    • v.22 no.1_2
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    • pp.35-43
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    • 1979
  • The present investigation has been made to study the deficiency symptoms of boron on the formation of cell wall and the development of the individual components of the orchid cell wall. Analytical samples were taken from two sources; one from the individual orchid plants started from an apical meristem culture followed by the generation of the protocorm-like body which was developed into a plant, the other from the plant cultivated in water for 30 days. The amount of boron in the cultrues were controlled and the deficiency symptoms were observed under theelectron microscope, optical microscope with samples taken from the zones of elongation of leaves and compared the dry weight of cell walls and finally the various fractions of the cell wall components. The following results were obtained: (1) The growth of roots and leaves was hampered in the boron deficient plants. (2) In the boron-deficient leaves a severe necrosis and cracks were developed in the tissue of zone of elongation besides the decrease in growth. (3) under the electorn microscope the cell walls of boron-deficient plants showed rough undulated structures unlike the smooth control cell walls. (4) the dry weight of total cells and cell walls of boron deficient plants were higher than the control plants. (5) In the boron deficient plant the amout of pectin and hemicellulose isolated from cell walls were higher and the amount of protein was lower than the controlled plots.

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SOME ASPECTS OF THE CANADIAN CULTURE OF GINSENG (PANAX QUINQUEFOLIUS L.), PARTICULARLY THE GROWING ENVIRONMENT

  • Proctor J.T.A
    • Proceedings of the Ginseng society Conference
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    • 1980.09a
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    • pp.39-47
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    • 1980
  • The harvesting and export of Canadian ginseng from the cool, shady hardwood forests of southern Canada can be traced to 1796. Because of its high dollar value and diminishing woodland supplies it was decided in 1896 that it should be cultivated under wood lath screens. Present day economics dictate changes in production techniques to allow for a decreasing supply of expensive labour. Traditional wooden lath screens have a surface area of wood of 70 per cent and permit light penetration of only about 18 per cent. Experimental woven black polypropylene shade has an estimated surface area of 72 per cent and permits light pentration of about 28 per cent. While differences in air and leaf temperatures under the two shade structures can be measured it is doubtful if these are great enough to cause differences in plant growth under the two structures. Shade grown ginseng had a low fresh and dry weight and total chlorophyll content (ratio of a to b was 3 to 1) comparable to other shade species. There was no differences in fresh and dry weight and chlorophyll content of leaves from plants grown under the two shade structures. Maximum net photosynthesis of leaves was 0.175 g $CO_2\;m^{-2}\;hr^{-1}$ and light saturation level was about $200{\mu}E\;m^{-2}\;s^{-1},$ or about 10 per cent of full sunlight.

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