• Title/Summary/Keyword: Total bacterial counts

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Effects of Packing Methods on Storage and Microbiology of Chilled Chicken Breast and Thigh Meats (냉장방법과 포장방법이 냉장계육의 저장성 및 미생물의 증식에 미치는 영향)

  • 박구부;송또준;이상진;김용곤;박태선;신택순;이정일
    • Korean Journal of Poultry Science
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    • v.24 no.1
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    • pp.9-15
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    • 1997
  • This experiment was carried out to investigate the effects on shelf-life of chilling and packing methods of cold chicken breast and thigh meats. Deboned chicken breast and thigh meats were packed by either vacuum or atmosphere packing method, and stored at -2˚C for 1, 3, 7, 11, 15, and 20 days. The thiobarbituric acid(TBA) values of all treatments were significantly increased as the storage period extended(P<0.05), however, TBA values of all treatments were remark-ably decreased at 15 days. TBA values of immersion chilled and vacuum-packed thigh meats were increased, but no difference between atmosphere and vacuum-packing methods was found. The volatile basic nitrogen(VBN) values at 1 day of storage were low, but they were increased as the storage period extended. The VBN values at 20 days after storage were from 12.25~19.28 mg %. Total bacterial counts tended to increase in all treatments, however, no significant difference was found in any treatment. Total plate counts of breast meat were higher than those of thigh meat as the storage period extended.

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Bacteriological Characteristic of Atrina pectinata and Ruditapes philippinarum under Non-refrigerated and Refrigerated Storage Conditions

  • Kang, Kyoung-Ho;Kim, Byeong-Hak;Kim, Young-Hun
    • The Korean Journal of Malacology
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    • v.24 no.1
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    • pp.25-32
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    • 2008
  • In order to estimate the necessity of refrigerated storage of fresh seafood for short-term storage, and evaluate the effect of refrigerated storage on pen shell Atrina pectinata and clam Ruditapes philippinarum collected from Jang-su of Deukryang Bay and I-mok of Sunchen Bay in South Korea, the counts of coliform, Escherichia coli and total aerobic bacteria in A. pectinata and R. philippinarum under non-refrigerated $(28{\pm}1^{\circ}C)$ and refrigerated storage conditions $(4{\pm}1^{\circ}C)$ were determined. The results indicated that the storage at temperature of $4^{\circ}C$ possessed significant effects on inhibiting bacterial growth in live seafood. And refrigerated storage had different effect on A. pectinata and R. philippinarum. Different species and culture environments significantly influenced the initial and ultima bacteria counts. This study confirmed that refrigerated storage for short-term storage of live seafood was necessary, and indicated that the effect of refrigerated storage was influenced by comprehensive effectors.

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Effect of Repetitive Redox Transitions to Soil Bacterial Community and its Potential Impact on the Cycles of Iron and Arsenic (비소오염토양에서 반복적인 Redox 환경 변화가 토양 미생물 군집과 비소 및 철의 순환에 미치는 영향)

  • Park, Sujin;Kim, Sanghyun;Chung, Hyeonyong;Chang, Sun Woo;Moon, Heesun;Nam, Kyoungphile
    • Journal of Soil and Groundwater Environment
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    • v.25 no.1
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    • pp.25-36
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    • 2020
  • In a redox transition zone, geochemical reactions are facilitated by active bacteria that mediate reactions involving electrons, and arsenic (As) and iron (Fe) cycles are the major electron transfer reactions occurring at such a site. In this study, the effect of repetitive redox changes on soil bacterial community in As-contaminated soil was investigated. The results revealed that bacterial community changed actively in response to redox changes, and bacterial diversity gradually decreased as the cycle repeated. Proportion of strict aerobes and anaerobes decreased, while microaerophilic species such as Azospirillum oryzae group became the predominant species, accounting for 72.7% of the total counts after four weeks of incubation. Bacterial species capable of reducing Fe or As (e.g., Clostridium, Desulfitobacterium) belonging to diverse phylogenetic groups were detected. Indices representing richness (i.e., Chao 1) and phylogenetic diversity decreased from 1,868 and 1,926 to 848 and 1,121, respectively. Principle component analysis suggests that repetitive redox fluctuation, rather than oxic or anoxic status itself, is an important factor in determining the change of soil bacterial community, which in turn affects the cycling of As and Fe in redox transition zones.

A study on the total cell count variation of commercial liquid seed by adding PAC and PAS (PAC 및 PAS 첨가에 따른 상업용 액상 종균제의 총균수 변화에 관한 연구)

  • 박미자;박경식;김승재
    • Journal of environmental and Sanitary engineering
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    • v.16 no.2
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    • pp.79-90
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    • 2001
  • Commercial liquid seeds are used for supplying active microbial flora to organic wastewater treatment plants of high feed-to-microorganism ratio and to maintain optimal microbial condition during unsteady state operation of the biological wastewater treatment plant. In addition to bacterial cells, the liquid weeds contain various additives for special purposes as well as organic substrates for energy supply. The additives give physical stability for the maintenance of microbial decomposition activity and ability to control the overgrowth of seed strains. In this work, the effects of addition of two kinds of typical substrate additives, poly aluminum chloride(PAC) and poly aluminum sulfate(PAS) on the consitutional total cell counts(CFU/ml) of four kinds of reorganization liquid seeds(RLS I, RLS II, RLS III and RLS IV) were studied experimentally. The addition of PAC and PAS gave negative effect on TCC constitution for the four seeds studied.

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Bacterial Counts in Ginseng Products by Dry Rehydratable Film Method (페트리 필림 방법에 의한 인삼제품의 세균수 측정)

  • 곽이성;장진규;이광승
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.41-43
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    • 1995
  • Dry rehydratable film (Petrifilm) method was compared with the standard pate count (SPC) method for estimation of total bacteria in ginseng products. Ginseng products (7 sample) was analyzed for total count by the SPC, and Petrifilm methods, respectively. In the case of ginseng tea, ginseng extract, ginseng extract pill, ginseng powder capsule, and ginseng extract tea, they showed non-significant values at the 1% level. However, the values of ginseng powder and tablet showed significant at the 1% level. These results generally indicate the suitability of the dry rehydratable film methods as alternatives to the SPC method for estimating of total bacteria in ginseng product samples except to ginseng powder and ginseng tablet.

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Physicochemical Characteristics and Storage Stabilities of Sauces with added Yuza(Citrus Junos) Juice (유자액을 이용한 소스의 이화학적 특성 및 저장성)

  • 유경미;서우영;서한석;김완수;박재복;황인경
    • Korean journal of food and cookery science
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    • v.20 no.4
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    • pp.403-408
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    • 2004
  • The purposes of this study were to investigate the physicochemical characteristics and storage stabilities of various sauces with added Yuza(Citrus Junos). Yuza consisted of three parts: peeled (46.17%), fleshed (43.74%) and seeded (10.09%). Yuza juice revealed 11.33% extraction yield, pH 2.85, 4.18% total acidity, 11 $^{\circ}$Brix, 1.16% transmittance and 2.63 $^{\circ}$Brix/acid ratio. Five kinds of sauces with added Yuza juice were Yuza red pepper paste sauce, Yuza soybean sauce for meat, Shabu shabu Yuza sauce, Korean Yuza vegetable sauce and Japanese Yuza soybean sauce. These sauces showed slight changes in pH, total acidity, total bacterial counts and overall sensory characteristics during storage period (60 days). In conclusion, Yuza juice could be applied to various sauces, which remained usable for 60 days.

Effectiveness of external agents in polluted sedimentary area

  • Alam, Md. Mahabub;Haque, Md. Niamul;Cho, Daechul;Kwon, Sung-Hyun
    • Environmental Engineering Research
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    • v.21 no.1
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    • pp.52-57
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    • 2016
  • Sediment is a useful natural source but deteriorated continually by anthropogenic and industrial sources. Therefore, it is imperative to search a suitable method for improving or restoring sediment quality. Sediment has been tested to identify the effects of some external agents on a polluted area for 28 days. Chemical analysis and total viable counts (TVC) test have been conducted for 4 days interval to assess their performance. The analyses of chemical oxygen demand (COD), acid volatile sulfide (AVS), total phosphorous (T-P), total nitrogen (T-N) indicate that the chemical agents was more efficient to improve sediment quality whereas the microbial agent was more efficient for nutrient releasing from sediment. Oxygen releasing property of the chemical agent was thought to be providing with more congenial environment for the higher growth of the bacterial community than the direct application of microbial agents.

Incidence and Causes of Sub-Clinical Mastitis in Dairy Cows on Smallholder and Large Scale Farms in Tropical Areas of Tanzania

  • Shem, M.N.;Malole, J.M.L.;Machangu, R.;Kurwijila, L.R.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.372-377
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    • 2001
  • A study on the prevalence and causes of sub-clinical mastitis was conducted on ten smallholder and large-scale dairy farms in Morogoro urban and peri-urban areas. A total of 65 lactating cows were screened using the California Mastitis Test (CMT). Confirmatory tests used included; the direct microscopic somatic cell count (DMSCC), culture, bacteriological and biochemical tests. Structured questionnaires were used to collect information on management aspects. Results showed 62% and 4% cows as sub- clinical and clinical mastitis cases respectively. Levels of infection were higher on smallholder farms (75%) than on large-scale farms (25%). All tested cows had high cell counts (>500,000) per ml of milk. Incidences of mastitis were significantly (p<0.05) related to milking practices. The dominant bacterial isolates in the same order were Staphylococcus aureus, Streptococcus spp, and Escherichia coli. Other organisms isolated included Pseudomonas spp and Klebsiella spp. It was concluded that the high rates of sub-clinical mastitis in the research area were mainly due to poor management and unhygienic milking practices.

Microbiological Evaluation of Commercial Eorigul-jeot, Salt-fermented Oyster Crassostrea gigas with Seasoning, Produced in Korea (국내산 시판 어리굴(Crassostrea gigas)젓의 미생물학적 평가)

  • Jae-Won So;Shin-Hye Lee;Kwon-Sam Park
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.2
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    • pp.122-128
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    • 2024
  • We investigated the quality of 10 commercial eorigul-jeot, salt-fermented oysters with seasoning, by measuring their chemical composition and bacterial concentrations. The Eorigul-jeot had 5.07-6.06 pH (mean, 5.63), of 1.92-4.74% salinity (mean, 3.36%), 7.01-14.70 mg/100 g volatile basic nitrogen (mean, 11.91 mg/100 g), 139.22-267.11 mg/100 g amino acid nitrogen (mean, 212.69 mg/100 g), and 1.02-1.65 g/100 g total acidity (mean, 1.24 g/100 g). The total viable and lactic acid bacterial counts were 5.7×104-8.7×105 and 2.7×103-2.0×105 CFU/g, respectively, and fecal coliform was detected in only one Eorigul-jeot sample. Bacillus cereus and Clostridium perfringens were detected in two samples, and all Eorigul-jeot samples were negative for Staphylococcus aureus and Vibrio parahaemolyticus. These results strongly suggest the need to monitor food-poisoning bacteria in commercial Eorigul-jeot to ensure consumer health.

A Study on Ingredient and Bacterial Contamination of Bean Curd on the Market (市販豆腐 成分 및 細菌汚染에 관한 硏究)

  • Hong, Ki-Wha
    • Journal of Environmental Health Sciences
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    • v.9 no.2
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    • pp.1-10
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    • 1983
  • The purpose of this study was to analyze general ingredient of bean cured on the market and to examine its exposure to bacterial contamination. For this study, 17 samples (each 9 samples for general bean curd, soft bean curd, and uncurdled bean curd) were randomly collected from nine areas in Seoul from the beginning of April, 1983 to the beginning of June, 1983. The result of ingredient analysis of moisture, ash, and protein of bean curd was compared with the standard set by the Ministry of Health & Social Affairs. In order to find out exposure of bean curd on the market to bacterial contamination, total biological bacteria and coliform group were examined. Experimental results were shown as follows 1) Results of ingredient analysis of moisture, ash, and protein of general bean curd showed that total samples in both moisture and protein met the standard set by the Ministry of Health & Social Affairs but 44.4% of the samples in ash was below the above standard, indicating average 82.0%, 0.9% and 9.6% in moisture, ash, and protein order. 2) Experimental results of moisture, ash, and protein of soft bean curd demonstrated 90.2%, 0.5% and 4.3% respectively total samples in both moisture and protein satisfied the self-criteria set by the Soft Food Co-operative Association of Seoul City but 11.1% of the samples in ash didn't meet the self-criteria. 3) Total samples of uncurdled bean curd satisfied the self-criteria set by the above association, indicating average 92.0%, 0.4%, and 3.5% in moisture, ash, and protein order. 4) Total biological bacteria and coliform group detected in general bean curd showed that more than 10$^5$/g in total biological bacteria accounted for 88.8% of the samples and that 10$^4$/g or more in coliform group accounted for 77.7% of the samples. The result proves that general bean curd has been exposured to a severe bacterial contamination. 5) Result of total biological bacteria and coliform group detected in such packed bean curd as uncurdled bean curd and soft bean curd showed that 61.6% of the samples exceeded 10$^6$/g in total biological bacteria and 27.7% of the samples exceeded 10$^3$/g in coliform group. 6) According to the change with time and temperatures in total biological bacteria and coliform group of general bean curd, general bean curd began to decay around 72 hours at 4$\circ$C and around 48 hours at 23$\circ$C and around 24 hours at 37$\circ$C and, at that time, total biological bacteria approached 10$^6$/g while coliform group did 10$^6$/g. The result indicates that temperature has a great effect on bacteria counts and decay.

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