• 한국식품과학회지
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• 제36권6호
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• pp.1032-1040
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• 2004

생식은 건강 지향적 식생활 양상의 변화로 최근 소비가 급증하고 있으나, 업체의 영세성에 따른 위생관리가 미비하며 제조공정의 표준화가 되어 있지 않아 안전성 문제가 대두되고 있다. 본 연구는 생식의 위생적 생산을 위한 생식 생산 공정의 HACCP 일반모델을 개발을 위해 생식 원료의 세척에서부터 최종제품 포장까지 각 제조공정별로 생물학적, 물리적, 화학적 요인의 위해요인을 분석하고 중요관리점(CCPs)를 설정하고자 하였다. 원료의 세척부터 최종제품 포장까지 각 제조공정별로 생물학적, 물리적, 화학적 요인의 위해요인을 분석하였다. 생식원료의 오염수준은 $2.82-8.23\;{\log}_{10}\;CFU/g$의 일반세균수가 검출되었으며, 또한 $1.40-6.57\;{\log}_{10}\;CFU/g$의 coliforms이 검출되었다. 최종제품 중 일반세균수는 $1.51-7.40\;{\log}_{10}\;CFU/g$이 검출되었다. 제조공정 중 미생물 수 변화는 모든 원료에서 공정별로 유사한 패턴을 보여 주었는데 세척과 동결건조공정에서 일반세균수와 대장균군은 감소하였다. 물리적 요인인 이물 혼입과 화학적 요인인 수분함량의 변화가 위해 요인으로 분석되었다. 생식의 제조공정 중 CCP는 미생물 감소를 위한 세척 단계, 수분 관리를 위한 건조단계, 그리고 금속 등 이물질의 혼입단계로 설정되었다.

• 한국축산식품학회지
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• 제38권6호
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• pp.1253-1260
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• 2018

Pig small intestine not only is used as food but also for sausage casings production in many countries worldwide. However, it is well recognized that the small intestine is important source of spoilage and pathogenic bacteria. The present study aimed at investigating the effects of different washing and packaging methods on the changes of microbial levels and physicochemical characteristics of pig small intestine. After collecting and trimming off of visible fats, the pig small intestine samples were treated with; (i) different packaging methods: aerobic packaging (AP), skin packaging (SP), and vacuum packaging (VP); and (ii) washing with different concentrations of acetic acid. The treated samples were then stored at $4^{\circ}C$ for 1, 4, 7, and 10 d. At 1-d storage, higher pH value was found in the AP-treated samples, however, after 7 to 10 days the samples treated with SP had higher values compared to the ones treated with AP and VP (p<0.05). Thiobarbituric acid reactive substances values were higher in the AP-treated samples than those of the SP- and VP- treated samples at 7-d storage (p<0.05). At $10^{th}$ d, total plate counts (TPC) were higher in the control than in the acetic acid-washed samples (p<0.05). Additionally, the TPC was lower in the SP- and VP-treated samples than the AP-treated samples at 7-d storage (p<0.05). These obtained results suggest that the applications of washing with acetic acid solution and/or SP and VP methods could be an effective way to extend the shelf-life of pig small intestine during cold distribution.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1653-1660
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• 2004

부산시내 초등학교 5곳을 방문하여 현 급식소에서 보유하고 있는 급식시설$.$설비에 대한HACCP 적용시 중점관리점을 파악하기 위하여 위생평가를 실시하였다. 학교 급식 시설$.$설비는 학교급식소에서 사용하고 있는 천장, 벽, 바닥, 트렌치, 그리스 트랩, 후드, 방충망, 덤웨이터 등 총 8곳으로 분류하여 총균수, 대장균군수 측정을 통해 위생상태를 확인하였다. 급식시설$.$설비 표면의 위생상태를 검사한 결과 천장, 벽을 제외한 나머지에서 $10^4$∼$10^{6}$ CFU/100 $\textrm{cm}^2$의 세균이 검출되어 여전히 학교급식소의 위생수준이 낮은 것으로 파악되었고 특히, 그리스 트랩의 경우가 가장 위생상태가 불량한 것으로 나타났다. 대장균군수의 경우는 천장을 제외한 나머지 장소에서 $10^2$∼$10^4$ CFU/100 $\textrm{cm}^2$의 세균이 검출되었고 그리스 트랩에서 가장 높은 수의 세균과 대장균이 발견되어 특별히 관리 해야 되는 부분으로 확인되었다. 또한 학교급식 시설$.$설비의 하절기 및 동절기의 위생상태를 알아보고 중점 관리 점을 제시하기 위하여 급식 후 세 척 및 소독을 실시한 다음 시간 및 요일의 경과에 따른 오염도를 측정한 결과, 하절기에는 매일 소독을 실시해야 하는 바닥, 트렌치, 그리스 트랩, 덤웨이터의 경우는 급식 전에 소독을 실시한 후 급식을 시작해야 하는 것으로 나타났고, 주 1회 소독을 실시 하는 벽, 후드, 방충망은 주 3회 이상으로 소독횟수를 늘려 실시해야 하는 것으로 나타났다. 동절기에는 바닥, 트렌치, 덤웨이터의 경우는 현재와 마찬가지로 급식 후에 소독을 실시하여야 하는 것으로 나타났고 그리스 트랩의 경우는 급식 전에 실시한 후 급식을 시작해야 하는 것으로 나타났다. 벽, 후드, 방충망은 주 2회 이상으로 소독횟수를 늘려 실시해야 하는 것으로 나타났다.

• 상하수도학회지
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• 제33권5호
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• pp.395-404
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• 2019

The point-of-use water dispenser systems are widely used because of convenience in handling and demand for high-quality drinking water. The application has been increased recently in the public places such as department stores, universities and the rest areas in express ways. Improvement of water qualities by the dispenser systems was compared with tap water in this study. The tap water is supplied to the dispenser as the influent of the dispenser system. The twelve dispensers in the public places were used. The five dispensers used reverse osmosis as the main filter and other dispensers used various filters such as ultrafiltration, nanofiltration, and alumina filter. The water quality indicators for sanitation safety, i.e., turbidity and total coliforms, were evaluated. Other water qualities such as pH, residual chlorine, heterotrophic plate count (HPC), and total cell counts were also analyzed. By the point-of-use water dispenser, the turbidity, residual chlorine and pH were decreased and the HPC and total cell counts were increased. The t-test results revealed that the HPC of the tap waters were not significantly different from the treated waters but the total cell counts of the two groups were significantly different. The low pH of the RO filter treatment was also significantly different from the tap waters. This study will contribute to understand the role of the point-of-use water dispenser in improving water quality and to identify key water quality for the proper maintenance of the dispenser systems.

• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1157-1163
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• 2014

This study was conducted to observe the effects of gas composition in modified atmosphere packaging (MAP) on the shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred ($KNP{\times}D$) during refrigerated storage. Muscle sample was obtained from the left side of carcass of seven months old of $KNP{\times}D$ barrow. The sample was sliced into 1 cm in thickness, placed on trays (two slices/tray) and filled with different gas composition, i.e. 0:20:80/$O_2:CO_2:N_2$ (MAP1), 30:20:50/$O_2:CO_2:N_2$ (MAP2) and 70:20:10/$O_2:CO_2:N_2$ (MAP3). Other slices of sample were vacuum packed (VP) as a control. All packs were stored at $5{\pm}1^{\circ}C$. At 12 d of storage, pH value of MAP2 and MAP3 were higher (p<0.05) than that of MAP1 and pH value of MAP1 was higher (p<0.05) than that of VP. At 6 d of storage, redness ($a^*$) value of MAP2 and MAP3 were higher (p<0.05) than that of VP and MAP1 and, at 9 and 12 d of storage, redness value of MAP3 was higher (p<0.05) than that of VP, MAP1, and MAP2. At 3, 6, 9, and 12 d of storage, the 2- thiobarbituric acid reactive substances (TBARS) value of MAP3 was higher than that of MAP2 and TBARS value of MAP2 was higher than that of VP and MAP1. At 3, 6, 9, and 12 d of storage, volatile basic nitrogen values of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1. At 3 d of storage, total aerobic plate counts of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1 and, at 6 d of storage, total aerobic plate counts of MAP3 was higher (p<0.05) than that of MAP1 and MAP2. However, there was no significant different total aerobic plate count among MAP1, MAP2, and MAP3 at 9 and 12 d of storage. There was no significant different total anaerobic plate count among MAP1, MAP2, and MAP3 during storage. It is concluded that the MAP containing 30:20:50/$O_2:CO_2:N_2$ gas composition (MAP2) might be ideal for better meat quality for $KNP{\times}D$ meat.

• 동아시아식생활학회지
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• 제14권3호
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• pp.265-274
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• 2004

To investigate the effect of cereal powders on the quality and storage characteristics of sausage, Aw, pH, microbial count, TBA value, VBN value, textual and sensory characteristics were examined. Water activity tended to decrease as storage time prolonged and all the samples showed higher Aw's than that of the control. During the storage period, pH values of all the samples decreased. L, a and b values had a tendency to decrease during the storage period. During the four weeks storage at 4$^{\circ}C$, TBA and VBN values of all the sample showed increasing tendency and were lower than those of the control. Total plate counts of bacteria, the number of lactic acid bacteria and coliform bacteria counts increased as the storage time prolonged. Texture characteristics of all samples increased up to 1 week storage and decreased subsequently. The sensory scores of all the samples decreased as the storage time prolonged.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.109-118
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• 2016

The antioxidant potential of mistletoe (Viscum album L. var. coloratum Ohwi; VAL) extract in uncooked pork patties was evaluated. Three concentrations of VAL extract (0.1 [T1], 0.5% [T2] and 1.0% [T3]) along with 0.02% ascorbic acid as a positive control (V) were added to ground pork and pork patties were prepared. Incorporation of VAL extract decreased (p<0.05) the pH of the pork patties throughout the storage time and reduced (p<0.01) the thiobarbituric acid reactive substance values after day 14 of storage. Total plate counts of the VAL extract-treated samples and V-treated samples were also significantly lower (p<0.01) than that of the control (C) throughout the storage period. In addition, odor scores of the VAL extract-treated patties were lower than those of the C- or V-treated samples on 3rd day of the storage period. These results demonstrated that the VAL extract acts as a natural antioxidant in uncooked pork products.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1114-1119
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• 2004

The biofilms on pipe walls in water distribution systems are of interest since they can lead to chlorine demand, coliform growth, pipe corrosion, and water taste and odor problems. As such, the study described in this paper is part of an AWWARF and Tampa Bay Water tailored collaboration project to determine the effect of blending different source waters on the water quality in various distribution systems. The project was based on 18 independent pilot distribution systems (PDS), each being fed by a different water blend (7 finished waters blended in different proportions). The source waters compared were groundwater, surface water, and brackish water, which were treated in a variety of pilot distribution systems, including reverse osmosis (RO) (desalination), both membrane and chemical softening, and ozonation-biological activated carbon (BAC), resulting in a total of 7 different finished waters. The observations from this study consistently demonstrated that unlined ductile iron was more heavily colonized by a biomass than galvanized steel, lined ductile iron, and PVC (in that order) and that the fixed biomass accumulation was more influenced by the nature of the supporting material than by the water quality (including the secondary residual levels). However, although the bulk liquid water cultivable bacterial counts (i.e. heterotrophic plate counts or HPCs) did not increase with a greater biofilm accumulation, the results also suggested that high HPCs corresponded to a low disinfectant residual more than a high biofilm inventory. Furthermore, temperature was found to affect the biofilms, plus the AOC was important when the residual was between 0.6 and 2.0 mg $Cl_2/l$. An additional aspect of the current study was that the potential of the exoproteolytic activity (PEPA) technique was used along with a traditional so-called destructive technique in which the biofilm was scrapped off the coupon surface, resuspended, and cultivated on an R2A agar. Both techniques indicated similar trends and relative comparisons among the PDSs, yet the culturable biofilm values for the traditional method were several orders of magnitude lower than the PEPA values.

• Food Science and Biotechnology
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• 제15권6호
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• pp.920-924
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• 2006

The microbiological safety of foodservice facilities and side dishes in 6 elementary schools and 6 universities in the Jeolla-do region was evaluated. The micro-aerosol evaluation of foodservice facilities including the kitchen, refrigerators, and freezers showed a comparatively high level of microbial contamination. In the microbial safety evaluation of cooking utensils and appliances, the total plate counts of serving tables, cutting boards, and food plates were comparatively high, but did not reveal significant counts of coliforms, Staphylococcus aureus, and Escherichia coli were both below the general limit of microbial contamination. The microbiological safety of the cooking utensils and appliances were satisfactory. In the microbiological safety evaluation of side dishes served at these foodservice facilities, microbial counts were generally below the limit of microbial contamination.

• 한국축산식품학회지
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• 제28권2호
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• pp.113-121
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• 2008

열풍건조 단감분말과 동결건조 단감분말을 각각 0%, 3%, 6%을 첨가하여 미트 패티를 제조한 후 $-18^{\circ}C$에 40일간 저장하면서 품질특성을 평가하였다. 저장기간이 경과함에 따라 모든 제품의 pH는 30일까지 유의적으로 낮았으나(p<0.05) 40일차에는 약간 증가하였다. 보수력은 대조구와 처리구 간에 유의적인 차이가 없었다(p>0.05). 가열감량은 저장기간이 경과함에 따라 유의적으로 증가하였으며(p<0.05), 단감분말이 첨가된 처리구들이 대조구보다 유의적으로 낮았다(p<0.05). 육색 측정결과, 저장기간이 경과함에 따라 명도(L), 적색도(a), 황색도(b) 모두 유의적으로 낮아졌다(p<0.05). 처리구와 비교 시 대조구의 명도가 유의적으로 높았고(p<0.05), 적색도와 황색도는 처리구들이 다소 높은 값을 보였다. 직경감소율은 저장기간이 길어질수록 증가하였다(p<0.05). 미생물 분석결과 저장 40일차까지 총균수는 4.20 log CFU/g를 유지하여 양호하였으나, T3 및 T4는 대조구보다 총균수와 대장균수가 높게 나타났다(p<0.05). VBN 분석결과 T4가 저장 15일차부터 40일까지 다른 제품들에 비해 유의적으로 높았고(p<0.05), TBARS는 대조구에 비해 처리구들이 모두 유의적으로 높게 나타났다(p<0.05). 관능평가에서 전체적인 기호도는 처리구가 대조구에 비해서 다소 높게 평가받았다. 이상의 결과로서 다양한 생리활성물질을 가지는 열풍 또는 동결건조 단감분말은 기능성 식품의 소재로서 미트패티류 제품에 적용할 수 있음을 확인하였다.