• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.8
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• pp.961-966
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• 2006

This study was worked out to investigate antioxidant activity of solvent extracts from wild grape skin by measuring electron donating ability (EDA), reducing power, superoxide dismutase (SOD)-like activity, thiobarbituric acid reactive substances (TBARS) and nitrite scavenging ability. Total phenolic compound and flavonoids contents were the highest in ethyl acetate extract, $54.4{\pm}1.18\;mg/100\;g$ and $645.1{\pm}5.05\;mg/100\;g$, respectively. The EDA and reducing power of solvent extracts from wild grape skin were proportionally increased with concentration and ethyl acetate extract $(79.2{\pm}0.06%)$ showed the stronger than BHT $(74.1{\pm}0.15%)$ at concentration of $100\;{\mu}g/mL$, especially. SOD-like ability of ethyl acetate $(25.1{\pm}0.41%)$ and butanol $(20.2{\pm}0.13%)$ extracts were stronger than other extracts at concentration of $100\;{\mu}g/mL$. TBARS of ethyl acetate extract was higher than ascorbic acid. Nitrite scavenging ability of solvent extracts from wild grape skin (pH 2.5, $1000\;{\mu}g/mL$) were in order of ethyl acetate $(90.5{\pm}0.75%)$>butanol $(65.9{\pm}2.16%)$>hexane $(58.1{\pm}1.74%)$>chloroform $(55.4{\pm}1.02%)$>water $(40.9{\pm}0.35%)$. Antioxidant activity of solvent extracts from wild grape skin was the highest in ethyl acetate extract from the results of our experiments.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.813-818
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• 2007

For this study, extracts of Vitex rotundifolia stems were prepared using reflux water extraction (WE), reflux ethanol extraction (EE) and hot water extract under high pressure (HWE). The extracts were investigated for the total content of phenolic compounds, antioxidant activities, and inhibitory potencies for xanthine oxidase and tyrosinase. The EE extraction method yielded the highest content of polyphenol compounds (176.34 mg/g). The electron donating abilities (EDA) were 93.46${\sim}$96.92%, when extracts were assayed at 1.0 mg/mL. The superoxide dismutase (SOD)-like activity was the highest in the WE extract (47.32% at 1.0 mg/mL). The nitrite scavenging abilities (pH 1.2) were 84.61${\sim}$88.36% and the inhibition of xanthine oxidase were over 90% at 0.5 mg/mL. Tyrosinase inhibition of HWE and WE were 57.84% and 53.47% respectively. It implies that V. rotundifolia stems have potent physiological activities and their activities were differently exhibited depending on solvent fractions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.11
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• pp.1478-1484
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• 2009

The antioxidant, antiproliferation, and nitrate synthesis inhibitory effects of Magnolia denudata extracts (ME) were evaluated. The ME was extracted with 70% (v/v) ethanol and fractionated with solvents of hexane, chloroform, ethyl acetate, n-buthanol and aqueous. The ethyl acetate fraction contained the highest phenolic and flavonoid contents of 427.10 mg garlic acid eq/g and 356.05 mg catechin eq/g, respectively. The ethyl acetate fraction showed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity with a 50% inhibition concentration ($IC_{50}$) of 0.20 mg/mL and total antioxidant activity was 0.90 mg AA eq/100 mg. From the results of cytotoxic effects of HCT116, NCL-H460, and HepG2 human cancer cells by MTT assay on the ME and its solvent fraction, chloroform fraction showed the highest cytotoxic effect ($IC_{50}$ value: 0.14, 0.37, and 0.41 mg/mL, respectively). Nitrate synthesis inhibitory effect of ME and its solvent fractions on nitric oxide synthase activity in LPS stimulated RAW 264.7 cells were decreased in dose-dependent manners, and $IC_{50}$ value of hexane and chloroform fractions were 0.39 and 0.49 mg/mL, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.8
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• pp.1179-1183
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• 2011

This study was conducted to investigate the quality characteristics, antioxidant activity, and ${\alpha}$-glucoamylase inhibitory activity of Dahyang, a Chungnam Agricultural Research & Extension Service's newly bred cultivar of brown button mushroom. Total phenolic compound contents of Dahyang and the no. 705 mushroom were 189${\pm}$12 mg% and 168${\pm}$8 mg%, respectively. The major free sugars in Dahyang were mannitol (3.11%), xylose (0.12%), and trehalose (0.08%). ${\beta}$-Glucan content was 28.34% in Dahyang and 26.55% in the no. 705 mushroom, respectively. Electron donating ability by DPPH in Dahyang and the no. 705 mushroom was 52.14% and 45.27% for the water extract, and 57.81% and 46.93% for the 80% ethanol extract, respectively. ${\alpha}$-Glucoamylase inhibitory activity in a 10 mg/mL concentration of water extract were was 33.25% in Dahyang and 29.22% in the no. 705 mushroom, respectively.

• Food Science and Preservation
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• v.18 no.4
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• pp.597-603
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• 2011

The in-vitro whitening and anti-wrinkle effect of ethanol extracts from apple flesh and peel were investigated. The EtOAc fractions from the ethanol extracts of apple flesh and peel showed in-vitro antioxidant activities in a dose-dependent manner on ABTS radical-scavenging activity and ferric-reducing/antioxidant power, and had the highest total phenolic contents (84.25 and 318.25 mg GAE/g). In addition, the EtOAc fractions generally showed strong UV absorption within the UV-B range. In the cellular system, the melanin synthesis of the B16/F10 melanoma cells was decreased by the EtOAc fractions of apple peel in a concentration-dependent manner. The EtOAc fractions of apple peel also showed a great elastase inhibition of 46.40% at 100 ${\mu}g$/mL, thus showing good in-vitro anti-wrinkle characteristics. These results suggest that the EtOAc fractions from the ethanol extract of apple peel can be used as whitening and anti-wrinkle agents as well as antioxidant resources.

• Food Science and Preservation
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• v.21 no.4
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• pp.565-572
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• 2014

This study was conducted to examine the antioxidant activities of extracts derived with different extraction methods (HE, hot water extraction; UE, ultrasonic extraction; and PE, pressured extraction) from Phellinuslinteus mycelium in Mori ramulus. The extraction yield of PE (7.73%) was higher than that of UE (6.03%) and of HE (5.44%). The total phenolic and flavonoid contents of PE were 9.87 g/100 g and 1.90 g/100 g, respectively. The oxygen radical absorbance capacity (ORAC) of PE ($769.63{\mu}M/g$ FW) was higher than that of HE ($622.96{\mu}M/g$ FW) and of UE ($249.06{\mu}M/g$ FW). The DPPH and ABTS radical scavenging activities of PE at $1,000{\mu}g/mL$ were 35.85% and 51.42%, respectively. The superoxide radical scavenging activity of PE, 29.16-93.89%, was higher than that of other extracts. The ferric-reducing antioxidant power and the reducing power of PE were $180.60-607.93{\mu}M$ and 0.14-0.51, respectively. The tyrosinase inhibition activity of PE (9.66-24.05%) improved with an increase in the treatment concentration. The antioxidant and tyrosinase inhibition activities of PE were significantly higher than those of the other extracts. In conclusion, we provided experimental evidence that Phellinus linteus mycelium extracts from Mori ramulus have potential as functional materials.

• Culinary science and hospitality research
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• v.17 no.1
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• pp.270-279
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• 2011

This study investigates the antioxidant activities of wild grape seed (Vitis coignetiea seed) extracts by solvents. Organic extracts of hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extracts of wild grape seed (Vitis coignetiea seed). Total phenolic compound contents of extracts from wild grape seed (Vitis coignetiea seed) by solvents were the highest in ethyl acetate extract, 64.9 mg/100 g. Electron donating abilities of organic solvent fraction obtained from 80% ethanol extracts of wild grape seed were proportionally increased with concentration, and the ethyl acetate extract(90.0%) showed stronger activities than BHT and Ascorbic acid at concentration of $100\;{\mu}L$/mL. Reducing power of organic solvent fraction obtained from 80% ethanol extracts of wild grape seed was the highest in the ethyl acetate extract(2.83) at concentration of $1,000\;{\mu}L$/mL. TBARS of the ethyl acetate extract was highest. Nitrite scavenging ability of organic solvent fraction obtained from 80% ethanol extracts of wild grape seed(pH 1.2, $1,000\;{\mu}L$/mL) was the highest in ethyl acetate extract(76.9%).

• Food Science and Preservation
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• v.23 no.7
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• pp.989-994
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• 2016

As part of our continuing search for bioactive natural products, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of an 80% methanolic extract and organic solvent soluble-portions of Aruncus dioicus var. kamtschaticus roots were investigated by using a bioassay system. The antioxidant activity of A. dioicus var. kamtschaticus roots extract and organic solvent soluble-portions were assessed by examining with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radical scavenging. In addition, anti-diabetic effects of the A. dioicus var. kamtschaticus root extract and organic solvent soluble-portions were tested via ${\alpha}$-glucosidase inhibition assay. The total phenolic contents of the products were determined by applying UV-VIS spectrophotometry. All tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory properties. In particular, the ${\alpha}$-glucosidase inhibitory and radical scavenging effects of the ethyl-acetate (EtOAc)-soluble portion from the roots of A. dioicus var. kamtschaticus were greater than those from other solvent-soluble portions. These results indicate that A. dioicus var. kamtschaticus could be considered a new effective source of natural antioxidants and anti-diabetic materials. More systematic research of the constituents of the roots of this A. dioicus variety will be conducted to further develop its antioxidative and anti-diabetic properties.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.47-53
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• 2010

Flowers and shoots of three species of Dendranthema boreale, Dendranthema indicum, Dendranthema zawdskii var. lucidum, were extracted with 80% ethanol by reflux, and polyphenol content, scavenging activity on DPPH and ABTS radicals, ferrous ion chelating effects and inhibition effects on lipid peroxidation were analyzed. Total polyphenol and flavonoid contents were highest in D. zawdskii var. lucidum, especially in the flower part. Scavenging activity on DPPH and ABTS was also highest in D. zawdskii var. lucidum flower with less activity in shoot. Ferrous ion chelating effects was highest with D. boreale flower and lowest in D. zawdskii var. lucidum flower. Inhibition activity on lipid peroxidation was highest in D. zawdskii var. lucidum shoot with 41.01% inhibition activity showing 32 days after reaction, which is higher than synthetic antioxidant BHT. Due to higher antioxidant level and activity of shoot and flower of D. zawdskii var. lucidum is promising material for natural plant antioxidant. It was also shown that antioxidant activity is different according to plant part ever in same plant, and proper plant species should be used for antioxidant after careful studies.

• Journal of Life Science
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• v.21 no.1
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• pp.62-67
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• 2011

This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on $\alpha$-glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The $\alpha$-glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.