• 한국물환경학회지
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• 제30권3호
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• pp.351-360
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• 2014

The allocation of margin of safety (MOS) at a uniform rate to all areas of the unit watershed makes it very difficult to keep the load allotment stable in the area for lack of reduction measures like forest land. This study developed an equation to calculate margin of safety differentially according to the regional characteristics. The equation was formulated on the basis of the regional characteristic factors such as a load contribution factor for land use type and a site conversion factor for the unit watershed. The load contribution factor represents a contribution of loads from a particular land use. The site conversion factor was derived from the site conversion ratio of a unit watershed. Margin of safety for the non-point pollution load in the land use sector decreased by 20~25% in three river basins. The margin of safety in the unit watersheds with low site occupation ratios decreased in high rate, while in the unit watersheds with large urban area decreased in low rate. With the application of the differential margin of safety considering regional characteristics, not only the reduction of pollution loads can become lighter but also it can be easier to develop plans for Total Maximum Daily Loads (TMDLs) even where the reduction measures are not available.

• 한국물환경학회지
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• 제32권6호
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• pp.670-699
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• 2016

In this study, 16 antibiotics were selected from among the top 30 veterinary antibiotics sold in South Korea in 2014, as well as from among the pharmaceuticals targeted by EPA method 1694, in order to review analytical methods for the detection of trace levels of antibiotics in environmental samples: surface water, soils, animal origin foods, and manures. LC-MS/MS was heavily used. In the chromatography for the detection of the selected antibiotics, the $C_{18}$ column was mostly used at the temperature of $30{\sim}40^{\circ}C$. Water and methanol/acetonitrile were commonly chosen as a nonpolar and a polar mobile phase, respectively. Gradient elution was applied to separate multiclass antibiotics. Volatile additives, such as formic acid, acetic acid, and ammonium acetate were mixed with the mobile phase to improve the ionization efficiency of analytes and the sensitivity in MS detection. Electrospray ionization (ESI) was widely used in the LC-MS/MS and positive ionization was preferred to determine the selected antibiotics. A protonated $[M+H]^+$ molecule was selected as a precursor ion, and its two transitions were analyzed, one for quantitative measurement and the other for confirmation. This study reviewed linearity of the calibration curve, recovery, repeatability, method detection limits (MDLs), and method quantification limits (MQLs) for each target compound used to validate the developed analytical methods.

• 한국물환경학회지
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• 제32권6호
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• pp.600-627
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• 2016

Korea is one of the countries with a large veterinary antibiotics market, although antimicrobial resistance in bacteria is becoming a serious issue in many countries. The Korean government started to take interest in estimating the effects of livestock manure on rivers and agricultural soils and in monitoring of heavy metals, organic pollutants and antibiotics in the ambient water and soil. In this paper, pre-treatment methods to separate the selected antibiotics from solid samples were reviewed. It is essential to select an efficient and appropriate procedure for pre-treatment due to the high proportion of proteins and organics in biosolid samples. Pre-treatment consists of extraction followed by clean-up. Initially, homogenized samples were extracted by sonication, mechanical agitation or pressurized liquid extraction with methanol/acetonitrile/water mixture under acidic/basic conditions depending on the compound. However, aminoglycosides and colistin were extracted with 5% trichloroacetic acid and HCl, respectively. Since the ${\beta}-lactams$ are easily decomposed in acidic and basic conditions, they were extracted in neutral pH. Filtration with a membrane (pore size, $0.2{\mu}m$) or solid phase extraction with HLB and methanol, as eluents, was normally applied for the clean-up. At least, three different pre-treatment procedures should be adopted to screen all the selected antibiotics in solid samples.

• 한국물환경학회지
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• 제32권6호
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• pp.628-648
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• 2016

Increased attention has been paid to the presence of veterinary antibiotics in various environmental matrices due to their toxicological behavior in the ecosystem and development of antibiotic-resistant strains of pathogenic bacteria. In the this review, 37 target antimicrobials were selected based on annual sales of antibiotics for livestock in South Korea 2014. Also, extraction and clean-up methods for the determination of the antibiotic residues in liquid samples including water, milk, and honey were comprehensively reviewed in the literature. Solid-phase extraction (SPE) was commonly used as a pre-treatment method for the samples. Most of the analytes were extracted in acidic conditions (2.5~4.0) except for aminoglycosides, which were extracted in neutral conditions (7.0~8.0). ${\beta}-Lactams$ showed the highest recoveries in neutral pH due to their degradation characteristics in acidic media. Starta-X, Oasis HLB, and Oasis MCX were frequently applied as an SPE cartridge and Oasis HLB showed the highest recoveries for the majority of antibiotic classes. The homogenized honey and milk were extracted by mixing with acids for deproteinization. Solids and other interfering substances in the extract were eliminated by centrifugation followed by membrane filtration or SPE before injection into HPLC.

• 한국물환경학회지
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• 제32권6호
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• pp.649-669
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• 2016

This study explored the analytical conditions for 21 veterinary antibiotics which have been popularly sold in South Korea in 2014 but have not yet been targeted in EPA method 1694. Most of the selected antibiotics were separated by a reverse-phase C18 column with a combination of (buffered) water and organic polar solvent, which was commonly methanol and acetonitrile in the gradient elution mode. Volatile additives such as formic acid, ammonium acetate and ammonium formate were usually added to the mobile phases to minimize asymmetrical and tailing of antibiotics' peaks and to increase their ionization in mass spectrometry. The analytical methods of aminoglycoside antibiotics were distinct from those of the other antibiotics in terms of adoption of ion-pair chromatography (IPC) and hydrophilic interaction liquid chromatography (HILIC) capable of retaining and separating extremely polar compounds due to their hydrophilicity. Trifluoroacetic acid or heptafluorobutyric acid was frequently added to the mobile phase as an ion-pair reagent for the IPC. Tandem mass spectrometry was numerously applied to the detection of antibiotics using positive electrospray ionization (ESI) and the selected reaction monitoring (SRM) mode. All reviewed analytical methods had been/were validated by evaluating recovery, limits of detection and quantification, decision limit or detection capability of the methods.

• 한국물환경학회지
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• 제33권6호
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• pp.769-779
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• 2017

Nutrient (i.e., nitrogen and phosphorus) budgets are required under a 'Livestock Excreta Survey'. A nutrient budget is one of the agri-environmental indicators that calculates the difference between the inputs and outputs of the amount of nutrients within a certain boundary and for a certain time period (e.g., 1 year). In this study, a nutrients budget model was developed to effectively determine the surplus of nutrients within a region in Korea. The C# program language was used in order to facilitate the deployment of a graphical user interface (GUI) and to enhance compatibility. Also, the model was developed on Windows OS, which is the commonly used operating system in Korea. The model was based on the OECD/Eurostat nutrient budget method, and it was modified to consider manure composting procedures as well. There are key features of the nutrient budget model, including directly use of the original data sets from various input and output sources, and a collectively exchange of the address in different formats. The model can quickly show the results of various spatial and temporal resolutions with the same data, as well as perform a sensitivity analysis with coefficients and easily compareresults using tables and graphs. Further, it would be necessary to study the extension of the scope of utilization, such as the application of various nutrient budget methods. It would also be helpful to investigate both pre and postprocessing information such as linking input data through online systems.

• Water Engineering Research
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• 제7권1호
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• pp.1-8
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• 2006

Recently, the population growth and agricultural development are rapidly undergoing in the South-West Texas. The junction of three river basins such as Lavaca river basin, Colorado-Lavaca Coastal basin and Lavaca-Guadalupe Coastal basin, are interesting for non-point and point source pollutant modeling: Especially, the 2 basins are an intensively agricultural region (Colorado-Lavaca Coastal/Lavaca-Guadalupe Coastal basins) and several cities are rapidly extended. In case of the Lavaca river basin, there are many range land. Several habitat types wide-spread over three relatively larger basins and five wastewater discharge regions are located in there. There are different hazardous substances which have been released. Total nutrient loads are composed of land surface load and river load as Non-point source and discharge from wastewater facilities as point source. In 3 basins region, where point and non-point sources of poll Jtion may be a big concern, because increasing fertilizers and pesticides use and population cause. This project objective seeks to how to assess and control the accumulation of non-point and point source and discuss the main impacts of agriculture and environmental concern as non-point source with water quality related to pesticides, fertilizer, and nutrients and as point source with wasterwater discharge from cities. The GIS technique has been developed to aid in the point and non-point source analysis of impacts to natural resource within watershed. This project shows the losses in $kg/km^2/year$ of BOD (Biological Oxygen Demand), TN (Total Nitrogen) and TP (Total Phosphorus) in the runoff from the surface of 3 basins. In the next paper, sediment contamination will show how to evaluate in Estuarine habitats of these downstream.

• 설비공학논문집
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• 제28권9호
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• pp.355-360
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• 2016

Continuing to the modeling of heating load, this paper, as the second part of consecutive works, presents LS-SVM (least square support vector machine) based model of winter time apartment hot water supply load in a district heating system, so as to be used in prediction of heating energy usage. Similar, but more severely, to heating load, hot water supply load varies in highly nonlinear manner. Such nonlinearity makes analytical model of it hardly exist in the literatures. LS-SVM is known as a good modeling tool for the system, especially for the nonlinear system depended by many independent factors. We collect 26,208 data of hot water supply load over a 13-week period in winter time, from 12 heat exchangers in seven different apartments. Then part of the collected data were used to construct LS-SVM based model and the rest of those were used to test the formed model accuracy. In modeling, we first constructed the model of district heating system's hot water supply load, using the unit heating area's hot water supply load of seven apartments. Such model will be used to estimate the total hot water supply load of which the district heating system needs to provide. Then the individual apartment hot water supply load model is also formed, which can be used to predict and to control the energy consumption of the individual apartment. The results obtained show that the total hot water supply load, which will be provided by the district heating system in winter time, can be predicted within 10% in MAPE (mean absolute percentage error). Also the individual apartment models can predict the individual apartment energy consumption for hot water supply load within 10% ~ 20% in MAPE.

• 한국물환경학회지
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• 제30권5호
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• pp.503-511
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• 2014

The temporal and spatial analyses of total nitrogen (TN) fractionation were conducted in order to understand 1) total nitrogen components in streams and 2) their patterns in rainy and dry seasons. The result showed that the concentration of nitrogen components in stream water was lower in non-urban area and getting higher in urban area. Dissolved total nitrogen (DTN) was 95~97.7% of total nitrogen in streams, and the proportion of dissolved organic nitrogen (DON) and ammonia nitrogen ($NH_3-N$) was higher with increasing urban area. The concentration of total nitrogen and nitrate nitrogen ($NO_3-N$) were highest in winter among four seasons. The result was showed that concentration of $NH_3-N$ was same variation as concentrations of TN and $NO_3-N$ in urban-rural complex and urban areas, except rural areas. During rainy season, concentrations of particulate organic nitrogen (PON) and $NH_3-N$ increased in rural areas and decreased in both urban-rural complex and urban areas. Correlation between total nitrogen components and land uses was positively correlated with site > paddy, and negatively correlated with forest. The variation of total nitrogen concentration was determined by $NO_3-N$ in non-urban areas, by $NO_3-N$ and $NH_3-N$ in urban-rural complex and by $NH_3-N$ in the urban areas.

• 한국물환경학회지
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• 제27권3호
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• pp.293-299
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• 2011

The quantity of a discharge load can change with changes in rainfall in the area with a combined sewer system (CSS). To evaluate the implementation appropriately in the management of total maximum daily loads (TMDLs), the effects of rainfall changes should be considered in the estimation of the discharge load. The rainfall condition for the estimation of the discharge load in a certain year should be standardized to the same rainfall condition as that of the reference year. However, the calculation process is very complicated with its potential limitations. This study investigated and developed relatively simple methods for estimating the discharge load. Load conversion method (LCM) is designed to convert the discharge load under the current rainfall condition into that of the reference rainfall conditions. Simple rainfall data method (SRDM) is to simplify the estimation process of the discharge load by the simple conversion of rainfall data. These methods were applied to calculate the discharge load and examine the estimation results. From the results of this study the application of these methods may be useful for estimating the discharge load in the TMDL process.