• 한방재활의학과학회지
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• 제29권4호
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• pp.29-45
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• 2019

Objectives This study is to investigate anti-obesity effects of Banggihwanggi-tang-hap-yeonggyechulgam-tang (BY), an herbal formula, in high fat diet induced obese mice model. Methods Fourty five male C57Bl/6J mice were randomly assigned to normal group fed with normal research diet (Nor, n=9), high fat diet control group treated with water (Veh, n=9), high fat diet group treated with orlistat (Oris; n=9, Orlistat 40 mg/kg), high fat diet group treated with low concentraion BY (BYL; n=6, BY 0.87 g/kg) and high fat diet group treated with high concentration BY (BYH; n=6, BY 1.74 g/kg). Results Seven weeks later, antioxidative capacity, body weight, epididymal fat pad and liver weight, reactive oxygen species (ROS), peroxynitrite ($ONOO^-$), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, triglyceride, high density lipoprotein (HDL), low density lipoprotein (LDL), superoxide dismutase (SOD), catalase, glutathione peroxidase (Gpx), heme oxygenase (HO)-1 and histology of liver were evaluated. In the BYH group, 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis (3 ethybenzothiazoline-6-sulfonic acid) radical scavenging activity were more than L-ascorbic acid. Body weight gain were significantly less than Veh group. Epididymal fat pad and liver weight gain were significantly less than Veh group. ROS and $ONOO^-$ were significantly less than with Veh group. ALT and AST were significantly less than with Veh group. Total cholesterol, triglyceride and LDL were significantly less, HDL were significantly more than Veh group. SOD, catalase, Gpx, HO-1 significantly increased compared with Veh group. Injury on liver was lesser than Veh group. Conclusions It can be suggested that BY has anti-obesity effects in high fat diet induced obese mice model.

• 운동영양학회지
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• 제15권4호
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• pp.163-171
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• 2011

The effects of a high-fat diet and fasting on resting energy expenditure and energy substrate utilization were examined using the method of measuring whole body energy metabolism and oxygen uptake. Eight 4-week old male Sprague-Dawley rats were used for the high-fat diet experiment. Energy metabolism was measured using acrylic metabolic chambers over 24 hours. After 1-week of preliminary feeding, 4 rats were fed a chow diet, whereas the remaining 4 rats were fed a high-fat diet (HF) ad libitum, which contained 40% (w/w, calorie base 60%) more fat than that in the chow diet. The flow rate to measure energy metabolism inside the chamber was controlled at a mean of 3.5 L/min, and five chambers were subjected to measurement. One of the five chambers was used to correct errors by measuring the atmosphere. As a result of 5 weeks of control diet and high-fat diet feeding, body weight of the high-fat diet group tended to increase more than that in the control diet fed group, but the difference was not significant. Oxygen uptake and carbon dioxide production changed as time went on over the 24 hr. The respiratory exchange ratio also changed during the 24 hr, and the difference between the groups was significant. The control group showed significantly more carbohydrate oxidation than that of the high-fat diet fed group. A fasting experiment was conducted using six 7-week old Sprague-Dawley male rats. Energy metabolism measurements were performed using the same method as that used in the high-fat diet experiment; resting metabolism was measured prior to fasting, and a fasting condition began from 9:00 am the next day for 3 days to calculate energy metabolism. Both body weight and 24-hour oxygen uptake decreased significantly as a result of 3-day fasting. Total oxygen uptake in the first day decreased, and declined significantly on day 3 of fasting. Total 24-hour carbon dioxide production decreased significantly over the 3 days. The mean 24-hour respiratory exchange ratio decreased significantly. Additionally, energy expenditure during the dark period (20:00-08:00), which is the active period for rats, decreased significantly with fasting, whereas energy expenditure during the light period (08:00-20:00) did not increase by fasting.

• Journal of Acupuncture Research
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• 제21권2호
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• pp.131-153
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• 2004

Objectives : This research was performed to investigate the effect of herbal acupuncture (Atratylodes japonica Koizumi and Coix lachrymajobi var. mayeun Stapf) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Methods : Experimental groups were divided into normal group(Sham), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture in ST40 SP9 treated group (AJ), high fat diet and Coix lachrymajobi var. mayeun Stapf-herbal acupuncture in ST40 SP9 treated group(CL), high fat diet and Atractylodes japonica+Coix lachrymajobi var. mayeun Stapf-herbal acupuncture in ST40 SP9 treated group(AJ+CL). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per day. Results : Body weight and food efficiency were decreased in AJ, AJ+CL. Feed intake was decreased in AJ, CL. The level of serum HDL-cholesterol was increased in AJ, CL. The level of serum total cholesterol, LDL-cholesterol and phospholipid were not significant. The level of serum triglyceride, free fatty acid were increased in AJ. The level of serum AST was decreased in AJ, AJ+CL. The level of serum ALT was decreased in AJ, CL, AJ+CL. The level of serum ALP was decreased in AJ+CL. The HDL to Total cholesterol ratio was increased in AJ. Conclusions : Atractylodes japonica-herbal acupuncture in ST40 SP9 can control the body weight, feed intake and feed efficiency ratio, HDL-cholesterol and herbal acupuncture combined Atractylodes japonica with Coix lachrymajobi var. mayeun Stapf is effective on Body weight, feed intake, feed efficiency ratio, protection of liver function and prevention cardiovascular risk by obesity.

• 한국식품영양과학회지
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• 제31권5호
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• pp.796-801
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• 2002

두충 에탄을 추출물이 생체내 지질대사 및 항산화 효소계에 미치는 영향을 조사하였다. 정상식이에 두충 추출물을 투여(CE)한 결과 총콜레스테롤은 대조군(C)에 비해 감소하는 경향을 나타내었으며 HDL은 증가하였으나 통계적 유의 성은 없었다 동맥경화 지수 역시 두충 추출물 투여군(CE)이 대조군 (L)에 비해 감소를 나타내었으며 HTR은 55%의 증가를 보였다(p<0.05. 고지방식이(CL)를 투여한 결과 혈청 콜레스테롤 함량은 대조군(C)에 비해 유의성있게(p<0.05) 증가하였으나 HDL과 HTR은 크게 감소되어 통계적 유의성 (p<0.05)을 나타내었다. 고지방식이군(CL)의 동맥경화지수(AI)는 대조군(C)에 비해 3.6배의 증가를 보였다(p<0.05). 고지방식이에 두충 추출물(CLE)을 투여했을 때 콜레스테롤 함량과 동맥경화지 수는 고지방식이군(CL)에 비해 감소하는 경향을 보였다. 고지방식이군(CL)의 중성 지방 함량과 인지질 함량은 대조군(C)에 비해 증가하는 경향을 나타내었으며 두충 추출물 투여(CLE)로 중성 지방은 CL군에 비해 크게 감소되었으며 (p<0.05) 인지질 또한 감소하는 경향을 보였다 간 지질대사에서 두충 추출물 투여군(CE)의 총 지질과 콜레스테롤은 대조군(C)에 비해 다소 감소하는 경향이었고 인지질은 대조군보다 22% 증가하였다. 고지방식이 (CL) 투여 시간의 총지질, 콜레스테롤, HDL, 중성 지방은 대조군(C)에 비해 유의성있게(p<0.05) 증가하였다. 고지방식이 (CL)에 의해 GST활성은 유의 성 있게 감소하였으나 catalase와 SOD활성은 대조군(C)과 차이를 보이지 않았다. 두충 추출물의 투여에 의한 CE및 CLE군의 GST와cata-lase활성은 각각 C와 CL군에 비해 증가하는 경향을 보였다.

• 대한지역사회영양학회지
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• 제8권3호
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• pp.356-367
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• 2003

This study was to investigate the effects of a silk peptide, and prune and raspberry mixture on the body fat, serum lipid composition and fat distribution in Korean women during the time they received these supplements. During the 4 month period of this research (June to October, 2002), 75 women (average age 22.9 yr) were selected as subjects. Their nutrient intake was investigated by use of a questionnaire and the 24-hour recall method. Antropometric assessments of the subjects were investigated by using the SBIA Method (Segmental Bioimpedance Assay, Inbody 3.0). The results were as follows: their mean body weight was 59.3 kg, their mean body height was 161.7 cm and their mean Basal Metabolic Indices (BMI) was 22.7. The status of their dietary fiber intake significantly increased (p < 0.05) after use of the supplements. Their body fat significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05), and their percent of body fat signiflcantly decreased after supplementation (p < 0.05). Their triceps and suprailiac circumference significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05). Their waist circumferences and waist-hip ratios (WHR) significantly decreased after both the $2^{nd}$and $3^{rd}$ stage of supplementation (p < 0.05). Their total cholesterol significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their LDL-cholesterol and LDL / HDL ratios significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their 4-HNE and total antioxidant statuses significantly decreased following the $2^{nd}$ and $3^{rd}$stage of supplementation, as compared to their baseline results and those obtained following the $1^{st}$ stage of supplementation (p <0.05). Their $2^{nd}$ stage supplementation results were significantly negatively correlated with their triceps measurements and WHR (p < 0.05). Their $3^{rd}$stage supplementation results were significantly negatively correlated with their anterior thigh measurements (p < 0.05). The above results show that prune, raspberry, silk peptide and dietary fiber supplementation to a routine diet improves body fat distribution, total cholesterol, and triglyceride levels. The decrease of abdominal fat and WHR were especially noteworthy. This implies a decrease of risk factors. (Korean J Community Nutrition 8(3) : 356~367, 2003)003)

• 동의생리병리학회지
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• 제23권3호
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• pp.685-694
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• 2009

This experimental study was designed to investigate the inhibitory effects of ethanol extract of modified Yukgunja-tang(mYGJT) on high-fat diet-induced obesity and hyperlipidemia in Sprague-Dawley rats, Animals were divided into normal, control, mYGJT(100 mg/kg and 200 mg/kg) treated groups. Obesity with hyperlipidemia was induced by high fat diet treatment for 6 weeks. mYGJT was given to the amimals by oral gavage for 4 weeks, starting at the high-fat diet regimen, The effect of mYGJT on the differentiation of 3T3 L1 adipocytes in vitro and serological paramamters for obesity and hyperlipidemia in vivo were evaluated, mYGJT significnatly inhibited the differentiation of 3T3 L1 adipocytes in a concentration dependent manner. mYGJT treatment siginficantly reduced body weight, abdominal and epididymal fat weight, and FER(Food Efficiency Ratio) compared with control group in a dose dependent manner. It also signficantly inhibited the levels of serum total lipid, triglyceride, phospholipid, total cholesterol, LDL, AI(Atherosclerosis Index) and returned the serum HDL to normal. Total lipids, triglycerides and cholesterols in the liver, as well as malondialdehyde(MDA) and hydroxy radical in the serum were significantly reduced. However, superoxide dismutase(SOD) activity was significantly increased in mYGJT treated group compared with control group. Finally, mYGJT treatment signficantly decreased the MDA and protein carbonyl concentrations of the hepatic homogenate but signficantly increased the activities of SOD, GSH-Px and Catalase. Taken together, these results suggest that mYGJT can be clinically useful in inhibiting high-fat diet-induced obesity and hyperlipidemia.

• 한국식품저장유통학회지
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• 제24권5호
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• pp.666-672
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• 2017

본 연구는 단삼식초 및 지구성 운동트레이닝이 고지방식 유도 비만 흰쥐의 신체구성 및 혈중 항목들의 농도 변화에 미치는 영향을 비교분석함으로서 대사성 증후군의 개선에 도움을 줄 수 있는 효과적인 방법을 모색하고자 실시되었다. 10주령 수컷 Wistar 흰쥐 40마리를 대상으로 8주간 고지방식 처치로 비만을 유도한 후 좌업군, 운동군, 단삼식초섭취군, 운동군+단삼식초섭취 복합처치군 등으로 무선, 배정하여 총 8주간 운동 및 식이처치를 실시하였으며, 체중 및 신체구성, 식이섭취량, 경구당 부하검사 및 혈중 콜레스테롤 농도 등을 측정하였다. 8주간의 단삼식초섭취는 지구성 운동과 병행할 경우 체중감소 및 인슐린 저항성의 개선효과를 확인할 수 있었다. 그러나 단삼식초섭취만 처치할 경우 오히려 체지방량을 증가시키고 제지방량은 감소시켰으며, 인슐린 저항성을 현저하게 개선시키지 않는 것으로 나타났다. 또한 지구성 운동과의 병행 시도의 경우에도 운동효과를 배제한 부가적인 효과가 나타나지 않았으며, 혈중 지질에 있어서는 운동의 효과마저 둔화시키는 것으로 나타났다. 따라서 단삼식초섭취에 따른 항고혈당, 항비만 효과는 확인될 수 없었으며, 추후 분자생물학적 분석에 의한 기전과 관련된 원인분석을 통해서 보다 명확한 결과를 제시할 수 있을 것으로 생각된다.

• 한방재활의학과학회지
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• 제15권2호
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• pp.91-104
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• 2005

Objectives : This study was performed to investigate the effect of herbal acupuncture(Angelica gigas, Astragalus membranaceus) at Chun-wan(CV-12) and Chok-Samni(ST36) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL-cholesterol to total cholesterol ratio of rats fed high fat diet for diet for 5 weeks. Methods : Experimental groups were divided into normal group, high fat diet group(Control), high fat diet and Angelica gigas herbal acupuncture at CV12 and ST36 treated group(HA-1), high fat diet and Astragalus membranaceus herbal acupuncture at CV12 and ST36 treated Group(HA-2), high fat diet and Astragalus membranaceus herbal acupuncture at CV12 and ST36 treated Group(HA-3). Herbal acupuncture was bilaterally treated at the level of 16.7 ul/kg body weight per day. Results : Body weight were significantly decreased on 15th and 20th day in HA-1, HA-2, and decresed on 25th and 30th day in all the groups. Food intake were increased on 20th day and after were decreased on 30th day in HA-1 and HA-2. And food intake of HA-3 were decreased on 10th day and after were increased on 20th and 25th day. Total cholesterol, LDL-cholesterol, triglyceride and ALT were significantly decreased in HA-1, HA-2 and HA-3 groups compared with control group. HDL-cholesterol to total cholesterol ratio were significantly increased in HA-1, HA-2 and HA-3 groups compared with control group. And food efficiency were significantly decreased in HA-1 and HA-3 groups compared with control group. Especially HDL-cholesterol were increased in HA-1 Group. Conclusions : Herbal acupuncture using Angelica gigas and Astragalus membranaceus at CV12 and ST36 can manage obesity by controlling body weight, food intake and food efficiency ratio.

• Journal of Community Nutrition
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• 제1권2호
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• pp.98-107
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• 1999

The purpose of this study was to investigate Bone Mineral Density(BMD) and affecting factors on BMD of college women in Seoul. The subjects were 47 healthy college women aged 18-25 years. Antrophometric and body fat measurements were performed by Bioelectrical Impedance Fatness Analyzer(Tanita TVF 202). Blood pressure and pulse frequency were measured. Dietary intakes and general living habits were examined through questionnaires and nutrient intakes were analyzed by Computer Aided Nutritional Analysis(CAN) program for professional. Serum total cholesterol, TG(triglyceride), HDL-cholesterol, total protein, albumin, GOT, calcium were measured by Spotchem(SP-4410). Serum osteocalcin and alkaline phosphatase(ALP) were measured to monitor bone formation. BMD of lumbar spine(L2-L4), right hip(neck, ward's triangle, trochanter) and right forearm were measured by Dual Energy X-ray Absorptiometry(DEXA). Muscle strength was measured by examining leg flexion strength(right and left), leg extension power(right and left), handgrip power(right and left) and back strength. All data were statistically analyzed by the SAS PC package program. BMD of college women was normal(by WHO, 1994). Their muscle strength was bad(by national fitness guidebook, 1995). Only a mall number of them exercised (32.6% of subjects). There was no significant difference among BMD, muscle strength and % body fat(p<0.05). There were significant differences between BMD and total cholesterol as well as TG and VLDL-cholesterol(p<0.05). Total cholesterol was associated with decreasing BMD of the right forearm(p<0.05). TG and VLDL-cholesterol are associated with increasing BMD of right hip ward's triangle(p<0.05). There were significant differences among BMD, ALP and serum total protein. ALP was associated with decreasing BMD of the right forearm(p<0.05). There were significant differences between BMD and Ca as well as between Na and K intakes (p<0.05). Intakes of Na and K were associated with decreasing BMD of the right forearm (UD)(p<0.05). There were significant differences between in BMD and pulse frequently and serum albumin (p<0.05). Serum albumin is associated with increasing BMD of L3-L4(p<0.05), right hip neck(p<0.05). %Body fat, TG, VLDL-C, Ca intake, pulse frequency and serum albumin were associated with increasing BMD(p<0.05). Intakes of Na and K, ALP, total cholesterol, total cholesterol, total protein and height are associated with decreasing BMD(p<0.05). Overall results indicate that Ca intake but to be moderate in protein and Na intakes in order to increase BMD. Body exercise was recommended to increase BMD as well.

• Journal of Nutrition and Health
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• 제35권7호
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• pp.743-753
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• 2002

The purpose of this study was to investigate the relationship among nutritional status, serum lipids and leptin of 134 elementary school students with different obesity index residing in Kyunggi-do, Korea. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum lipid and leptin levels were evaluated based on 24-hr dietary recalls, anthropometric measurements and blood analysis. The mean weight, height and obesity index of total subjects was 150.8cm, 45.6kg and 5.4%. The mean % of body fat was higher while LBM and TBW (total body water) were lower in over weight subjects than those of other two groups. Total food, plant protein, carbohydrate, and vitamin C intakes of underweight male students were lower than those of overweight male students. The consumption of total fat was the highest in the female normal weight subjects and the lowest in female overweight subjects. Male students consumed more plant protein and vitamin C than female students. The mean daily intake of vitamin B$_2$, calcium and zinc of all subjects did not meet the Korean RDA. Also, iron intake of female students was lower than the Korean RDA. The mean level of serum leptin was the highest in male and female overweight subjects (p＜0.001). The HDL-cholesterol level was the highest in the underweight group of male subjects (p＜0.05) and in the normal weight group of female subjects (p＜0.001) The serum level of leptin and atherogenic index (AI) of females were higher than those of males. Serum leptin of total subjects had significantly positive correlation with obesity index, weight, SBP, body fat, cholesterol intake, serum triglyceride, and AI. However, serum leptin was negatively correlated with LBM, TBW, intake of plant protein, vitamin B$_1$, vitamin C and serum HDL-cholesterol. Obesity index was also positively correlated with weight, SBP, body fat, serum triglyceride and AI. To summarize the results, the vitamin B$_2$, calcium, zinc, iron and fiber intakes should be supplied to elementary school students, especially those who were overweight. The average serum leptin level of students showed remarkably positive correlation with obesity index, body fat, serum lipids and AI. Also, it was suggested that the serum leptin might be related to the dietary factors like cholesterol, plant protein, vitamin B$_1$ and vitamin C of elementary school students.