• Title/Summary/Keyword: Tonin

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Development of Quantitative Extraction Method of Amygdalin without Enzymatic Hydrolysis from Tonin(Persicae Semen) by High Performance Liquid Chromatography

  • Hwang, Eun-Young;Lee, Sang-Soo;Lee, Je-Hyun;Hong, Seon-Pyo
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.453-456
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    • 2002
  • Tonin(Persicae Semen) is the herb medicine that contains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde, HCN, and glucose by emulsin, a hydrolysis enzyme in tonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula to nin was 0.1 % from crude powders, 1.4% from small pieces, 3.5% from half pieces and 2.4% from whole pieces. The extraction yield of amygdalin of outer shell-eliminated to nin was 0.3% from crude powders, 1.4% from small pieces, and 3.5% from half pieces and whole pieces respectively. The extraction yield of amygdalin was most high when using the size larger than half.

Development of Quantitative Extraction Method of Amygdalin without Enzymatic Hydrolysis from Tonin(Persicae Semen) by High Performance Liquid Chromatography

  • Lee, Sang-Soo;Hwang, Eun-Young;Koh, Jung-A;Kim, Dong-Min;Lee, Je-Hyun;Lee, Yong-Moon;Hong, Seon-Pyo
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.389.2-389.2
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    • 2002
  • Tonin(Persicae Semen)is the herb medicine that cntains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde. HCN. and glucoseby emulsin. a hydrolysis enzyme intonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula tonin was 0.1 % from crude powers. 1.4% from small pieces. 3.5% from half pieces and 2.4% from whole pieces. (omitted)

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FT-IR Study of Dopant-wool Interactions During PPy Deposition

  • Varesano Alessio;Aluigi Annalisa;Tonin Claudio;Ferrero Franco
    • Fibers and Polymers
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    • v.7 no.2
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    • pp.105-111
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    • 2006
  • Coating the fibre surface by in situ oxidative chemical polymerisation of polypyrrole (using $FeCl_3$ as oxidant) is a readily industrial applicable way to give electrical properties to wool with good ageing stability [1], although pre-treatments are required to avoid damage of the cuticle surface due to the acidic condition of the process. FT-IR and EDX analysis reveal that organic sulphonates and sulphates, used as dopants, are absorbed by wool, while chlorine ions are preferably embedded on the polypyrrole layer. The resulting electrical conductivity seems mainly due to the presence of chlorine as counter-ion of polypyrrole; nevertheless, the presence of arylsulphonate in the polymerisation bath increases the electrical conductivity of the coating layer.

Influence of Toxoplasma gondii Acute Infection on Cholinesterase Activities of Wistar Rats

  • Tonin, Alexandre Alberto;da Silva, Aleksandro Schafer;Thorstenberg, Maria Luiza;Castilhos, Livia Gelain;Franca, Raqueli Teresinha;Leal, Daniela Bitencourt Rosa;Duarte, Marta Maria Medeiros Frescura;Vogel, Fernanda Silveira Flores;de La Rue, Mario L.;dos Anjos Lopes, Sonia Terezinha
    • Parasites, Hosts and Diseases
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    • v.51 no.4
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    • pp.421-426
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    • 2013
  • Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.

Susceptibility of Mice to Trypanosoma evansi Treated with Human Plasma Containing Different Concentrations of Apolipoprotein L-1

  • Da Silva, Aleksandro S.;Fanfa, Vinicius R.;Otto, Mateus A.;Gressler, Lucas T.;Tavares, Kaio C.S.;Lazarotto, Cicera R.;Tonin, Alexandre A.;Miletti, Luiz C.;Duarte, Marta M.M.F.;Monteiro, Silvia G.
    • Parasites, Hosts and Diseases
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    • v.49 no.4
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    • pp.427-430
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    • 2011
  • The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a strain of T. evansi and human plasma (plasmas 1, 2, and 3) from 3 adult males clinically healthy were used. In vivo test used 50 mice divided in 5 groups (A to E) with 10 animals in each group. Animals of groups B to E were infected, and then treated with 0.2 ml of human plasma in the following outline: negative control (A), positive control (B), treatment with plasma 1 (C), treatment with plasma 2(D), and treatment with plasma 3 (E). Mice treated with human plasma showed an increase in longevity of $40.9{\pm}0.3$ (C), $20{\pm}9.0$ (D) and $35.6{\pm}9.3$ (E) days compared to the control group (B) which was $4.3{\pm}0.5$ days. The number of surviving mice and free of the parasite (blood smear and PCR negative) at the end of the experiment was 90%, 0%, and 60% for groups C, D, and E, respectively. The quantification of APOL1 was performed due to the large difference in the treatments that differed in the source plasma. In plasmas 1, 2, and 3 was detected the concentration of 194, 99, and 115 mg/dl of APOL1, respectively. However, we believe that this difference in the treatment efficiency is related to the level of APOL1 in plasmas.