• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.2
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• pp.296-305
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• 1998

A study was performed to evaluate an effect of toluene or ethanol pretreatment on the toluene metabolism. A slight liver damage in rats was induced by administration of 0.2 ml of toluene or 0.2 ml of 50% ethanol per 100 g of body weight intraperitoneally every other day for four weeks except the last day before sacrifice. One day before sacrifice, toluene was administered to rats pretreated ethanol or toluene and the control. Rats were sacrificed at the 1st, the 2nd, the 3rd and the 4th week after the first administration of xenobiotics mentioned above. Based on the histopathological findings, liver weight per body weight, serum alanine aminotransferase and hepatic glutathione content, toluene- or ethanol-pretreated groups showed the reversible liver injury. By the treatment of one dose of toluene, the contents of hippuric acid in urine was higher in the group pretreated with toluene or ethanol than control. The contents of cytochrome P-450, benzylalcohol dehydrogenase and benzaldehyde dehydrogenase activities were generally more increased in toluene- or ethanol-pretreated rats than control. $K_m$ values of the benzylalcohol dehydrogenase in pooled liver samples from toluene- and ethanol-pretreated groups were similar each other. $V_{max}$ values of toluene- or ethanol-pretreated group was higher than control. In conclusion, the toluene metabolism is accelerated in rats pretreated with toluene or ethanol.

• Journal of the East Asian Society of Dietary Life
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• v.6 no.3
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• pp.289-294
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• 1996

To evaluate an effect of acute ethanol pretreatment on the liver injury in toluene-treated rats, toluene(50% in olive oil) was intraperitoneally given four times at 0.3ml/100g body weight at interval of one day to the ethanol-pretreated rats(0.3ml of 50%/100g body weight). The increasing rate of liver weitht per body weight(%), serum levels of alanine aminotransferase (ALT) activity, liver lipid peroxide content was higher in toluene treated rats pretreated with ehtanol than those treated with toluene alone. Concomitantly the hepatic glucose-6-phosphatase activity was increased whereas glutathione content was decreased by ehtanol pretreatment before toluene administration in rats. In case of direct administration of acetaldhyde or benzaldehyde to the rats, the liver weitht per body weight(%) and serum levels of ALT activity were almost higher than the control group. There results indicate that the toluene treated rats showed the reversible injury of liver and intensified. however, by the ethanol treatment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.976-980
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• 1996

To elucidate the effect of acute ethanol pretreatment on some toluene metabolizing enzyme activities, rats were divided into 4groups: control, alcohol-treated, toluene-treated, rat's and toluene-treated rats pretreated with ethanol. The alcohol or toluene-treated rats showed the significantly increased activities of hepatic aniline hydroxylase(AH) and aminopyrine demethylase(AD) compared to the control group. And the toluene-treated rats pretreated with ethanol showed somewhat decreased tendency of these enzyme activities compared to only toluene-treated rats. Liver benzylalcohol or aldehyde dehydrogenase activities were higher in alcohol or toluene-treated rats than those of the control group. The toluene-treated rats showed the decreased tendency of benzylalcohol dehydrogenase activities by the pretreatment of alcohol. Furthermore, toluene treated-rats showed the markedly decreased activity of benzaldehyde dehydrogenase by the ethanol pretreat-ment. On the other hand, hepatic xanthine oxidase activity in toluene-treated animals pretreated with ethanol was significantly higher than those of the toluene alone-treated rats. These results indicate that the combination of ethanol with toluene treatment for a short period of time possibly results in decreased activity of some toluene metabolizing enzymes in rats.