• Asian-Australasian Journal of Animal Sciences
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• v.9 no.5
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• pp.591-595
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• 1996

This paper presents results from a study of the age and weight at puberty of Thai does. A randomized block $3{\times}3$ factorial design was used. Factors were genotype (Thai Native; TN, 75% TN $\times$ 25% Anglo-Nubian; AN and 50 TN $\times$ 50% AN does), year of kidding (1989, 1990 and 1991) and birth type (single or twin) as a block. It was shown that there were no significant (p > 0.05) difference in age at puberty among genotypes ($167.6{\pm}6.0$, $157.6{\pm}7.0$ and 160.0 = 4.7 days for TN, 75% TN $\times$ 25% AN and 50% TN $\times$ 50% AN does, respectively) and between birth types. However, does kidded in 1991 had significantly (p < 0.01) longer age at puberty ($194.0{\pm}7.4$ days) than did does kidded in 1989 ($143.0{\pm}4.5$ days) or in 1990 ($148.1{\pm}5.7$ days). There was an interaction effect between genotype and year. Fifty % TN $\times$ 50% AN does had significantly (p < 0.05) higher weights at pubery ($17.2{\pm}0.4kg$) than those of TN($14.3{\pm}0.5kg$ and 75% TN $\times$ 25% AN ($14.4{\pm}0.6kg$) does. There was no significant difference in weights at puberty between TN and 75% TN $\times$ 25% AN does. There was no effect of birth type on weight at puberty. There was an interaction effect between genotype and year of kidding on age and weight at puberty and on growth rate from birth to weaning.

• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.217-228
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• 2009

Mobile genetic segments, or transposons, are also referred to as jumping genes as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the insertion of a gene of interest at a specific chromosomal site, or the creation of knockout mutants. The former situation includes the integration of conjugative transposons via site-specific recombination, several transposons preferring a target site of a conserved AT-rich sequence, and Tn7 being site-specifically inserted at attTn7, the downstream of the essential glmS gene. The latter situation is exploited for random mutagenesis in many prokaryotes, including IS (insertion sequence) elements, mariner, Mu, Tn3 derivatives (Tn4430 and Tn917), Tn5, modified Tn7, Tn10, Tn552, and Ty1, enabling a variety of genetic manipulations. Randomly inserted transposons have been previously employed for a variety of applications such as genetic footprinting, gene transcriptional and translational fusion, signature-tagged mutagenesis (STM), DNA or cDNA sequencing, transposon site hybridization (TraSH), and scanning linker mutagenesis (SLM). Therefore, transposon-mediated genetic engineering is a valuable discipline for the study of bacterial physiology and pathogenesis in living hosts.

• BMB Reports
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• v.54 no.7
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• pp.374-379
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• 2021

Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.3
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• pp.255-261
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• 1995

This paper presents results from a study of the effects of age and sex on body and carcass composition of different goat genotypes. A completely randomized $3{\times}3{\times}2$ factorial design was used. Factors were genotype (Thai Native; TN, $50%TN{\times}50%$ Anglo-Nubian (AN) and $25%TN{\times}75%AN$ goats), age ($206{\pm}8$, $349{\pm}9$ and $428{\pm}8days$ or 6.9, 11.6 and 14.3 months, respectively) and sex (male and female). It was shown that TN kids had significantly higher (p < 0.05) hind percentage (7.9%) compared with $50%TN{\times}50% AN$ (7.2%) and $25%TN{\times}75%AN$ (7.1 %) kids. TN kids had a significantly (p < 0.05) higher heart percentage (0.44%) than that of $25%TN{\times}75%AN$ kids (0.38%). However, there was no significant difference in heart percentage between TN and $50%TN{\times}50%AN$ and between $50% TN{\times}50%AN$ and $25%TN{\times}75%AN$ kids. TN and $50%TN{\times}50%AN$ kids had significantly higher (p < 0.05) carcass muscle content (63.1 and 63.7%, respectively) than did $25%TN{\times}75%AN$ kids (59.9%). Kids at 6.9 months of age had significantly higher percentages of hide, feet, intestinal tract, liver, heart, kidneys and carcass bone than those of kids at 11.6 and 14.3 months of age. There were no significant differences in these characteristics between kids at 11.6 and 14.3 months of age. However, kids at 11.6 and 14.3 months of age had significantly higher percentages of omental fat, dressing carcass, muscle, total fat (subcutaneous + intermuscular + kidney and pelvic fat), muscle to bone ratios (MBR), and muscle plus fat to bone ratios (MFBR) than those of kids at 6.9 months. Males had significantly higher gut contents, but lower percentages of liver, omental fat and heart. Prediction equations were developed relating empty body weight (EBW) to hot carcass weight and some dissectible carcass fractions.

• Tuberculosis and Respiratory Diseases
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• v.59 no.1
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• pp.53-61
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• 2005

Background : Cardiac troponin I (cTnI) is a specific marker of myocardial injury. It is known that a higher level of cTnI is associated with a poor clinical outcome in patients with acute coronary syndrome. An elevation in cTnI is also observed in various noncardiac critical illnesses. This study evaluated whether cTnI is useful for predicting the prognosis in noncardiac critically ill patients. Methods : From June 2003 to July 2004 at Gyeongsang National University Hospital, we enrolled 215 patients (male:142, female:73, mean age:$63{\pm}15$ years ) who were admitted for critical illness other than acute coronary syndrome at the medical intensive care unit(ICU). The severity score of critical illness (SAPS II and SOFA) was determined and serum cTnI level was measured within 24 hours after admission to the ICU. The mortality rate was compared between the cTnI-positive (${\geq}0.1{\mu}g/L$) and cTnI-negative ($cTnI<0.1{\mu}g/L$) patients at the $10^{th}$ and $30^{th}$ day after admission to the ICU. The mean cTnI value was compared between the survivors and non-survivors at the $10^{th}$ and $30^{th}$ day after admission to the ICU in the cTnI-positive patients. The correlation between cTnI and the severity of the critical illness score (SAPS II and SOFA) was also analyzed in cTnI-positive patients. Results : 1) The number of cTnI-negative and positive patients were 95(44%) and 120(56%), respectively. 2) The mortality rate at the $10^{th}$ and $30^{th}$ day after admission to the ICU was significantly higher in the cTnI-positive patients (29%, 41%) than in the cTnI-negative patients (12%, 21%)(p<0.01). 3) In the cTnI-positive patients, the mean value of the cTnI at the $10^{th}$ and $30^{th}$ day after admission to the ICU was significantly higher in the non-survivors ($4.5{\pm}9.2{\mu}g/L$, $3.5{\pm}7.9{\mu}g/L$) than in the survivors($1.8{\pm}3.6{\mu}g/L$, $2.0{\pm}3.9{\mu}g/L$) (p < 0.05). 4) In the cTnI-positive patients, the cTnI level was significantly correlated with the SAPS II score (r=0.24, p<0.001) and SOFA score (r=0.30, p<0.001). Conclusion : The cTnI may be a useful prognostic marker in noncardiac critically ill patients.

• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.251-257
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• 2001

This study was carried out to investigate the effect of breed, parity, litter size, lactating period, and estrus interval on sow productivity traits in swine. Data from 492 heads of Landrace, Yorkshire or crossbred sow which were raised at Yonam College of Agriculture from March, 1998 to July, 2000 were analyzed for this study. The total number of pigs born (TN), the number of pigs born alive (NB), and the number of pigs suckled (NS) were greater in the crossbred sows than in the purebred. In TN and NB, the 3rd to 7th parities were greater than other parities, and the 8th parity was the lowest. The number of pigs stillbirthed (NSB) and the number of runt per litter tended to increase with the increase of TN. In addition, statistical analysis showed that parity had significant effect on most of traits. The current TN had highly significant effect on TN, NB and NS of the next parity As current TN increased, TN, NB, and NS of the next parity increased. The current lactating period also affected significantly for TN, NB, and NS of the next parity The sows which had the lactating period of 20∼21 days produced the greatest TN and NB in the next parity. Weaning to estrus interval(WEI) had significant effect on TN, NB, and NSB. Among WEI groups, the WEI group of 7∼13 days was the lowest in TN, NB and NSB.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.11 no.12
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• pp.1133-1138
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• 1998

In this paper, we obtained the monodomain alignment of nematic liquid Crystal(NLC) in the cell fabricated by transcription alignment method on polyimide(PI) surface with side chain. It is considered that the LC alignment produced by the transcription alignment method is attributed to a memory effect of the NLC on PI surfaces. Also we observed that the generated pretilt angle of NLC is about $3.7^{\circ}$ with transcription alignment on PI surface. Next, we measured that the voltage-transmittance characteristics of transcription-aligned TN-LCD are almost same compared to rubbing-aligned TN-LCD. Also, we measured that the curve of transcription-aligned TN-LCD is less sharp than that of the rubbing-aligend TN-LCD in the decay time characteristics. It is considered that the response time characteristics of transcription-aligned TN-LCD are attributed to the weak anchoring strength between the LC molecules and the polymer surface.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.305-311
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• 1988

The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168 $spr^{r}$ chromosome at the frequency of $1.0\times 10^{-5}-5.0\times 10^{-7}$ and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin ($50{\mu}$g/ml) and spectinomycin ($100{\mu}$g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.2
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• pp.99-110
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• 2005

Characteristics of concentrations of total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) pollutant in percolated water at four paddy field sites (Soro, Odong, Munui, and Boeun) were investigated by a nonparametric test. Percolation rate measurement and percolated water sampling were taken during irrigation periods at $5{\sim}10$ day intervals. The normality of percolation rate and pollutant concentrations were examined using histogram, boxplot, and the Kolmogorov-Smirnov (K-S) test. Pollutant concentrations in percolated water showed positively skewed distribution. The median concentrations of pollutant were 1.91 mg/L for TN, 0.021 mg/L for TP, and 6.6 mg/L for COD, which were lower than its arithmetic mean concentrations by $35\%$ for TN, $36\%$ for TP, and $13\%$ for COD. The median concentrations of TN and TP differed significantly among sample sites according to the Kruskal-Wallis test. However, median concentrations were not significantly different among month except for TN and TP of Soro and COD of Odong. The percolation load of pollutants during irrigation periods in the study area were estimated at $3.12{\sim}7.75\;kg/ha$ for TN, $0.033{\sim}0.155\;kg/ha$ for TP, and 10.7 kg/ha for COD, which were much lower than respective values reported in Japan.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.3
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• pp.65-71
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• 2010

This study was conducted to investigate the dynamics of nutrients such as total nitrogen (TN), nitrate nitrogen ($NO_3$-N) total phosphorous (TP), and phosphate phosphorous ($PO_4$-P) in outflow from a cabbage farmland in a mixed land-use watershed. The TN concentrations in groundwater showed twice peaks in late July 2006 and late March 2007 (3.8, 4.7 mg/L, respectively), when it rained shortly after fertilizer application, indicating that nitrogen leaching is greatly influenced by fertilization and rainfall. The mean concentrations of TN and $NO_3$-N in surface water were not significantly higher than those in groundwater, while the mean concentrations of TP and $PO_4$-P in surface water were significantly (p < 0.05) were higher than those in groundwater. The TN concentrations in groundwater were generally higher than those in surface water during fertilization and early growing season due to the effect of fertilization, but vice versa in the other periods. In contrast, the TP concentrations in groundwater were always lower than those in surface water due to the sorption of particulate phosphorous by soil. The ratio of TN load in baseflow to that in total TN load (39 %) was much greater than the TP ratio (7 %), suggesting that baseflow contribute to nitrogen export. Therefore, proper fertilization management should be taken to reduce nitrogen load through baseflow.