• Title/Summary/Keyword: Tn5

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Estimation Model of the Change in Dairy Leaf Surface Temperature Using Scaling Technique

  • Eom, Ki-Cheol;Eom, Ho-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.5
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    • pp.359-364
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    • 2013
  • This study was conducted to develop a model to estimate crop leaf surface temperature. The results were as following; A definition for the daily time based on elapsed time from the midnight (00:00) as "E&E time" with the unit of Kmin. was suggested. The model to estimate the scaled temperature ($T^*e$) of crop leaf surface temperature by scale factor ($T^*$) according to the "E&E time : Kmin."(X) was developed as eq. (1) $T^*e=0.5{\cdot}sin(X+780)+0.5$ (2) $T^*=(Tx-Tn)/(Tm-Tn)$, Tx : Daily leaf temperature, Tm : Daily maximum leaf temperature, Tn : Daily minimum leaf temperature. Relative sensitivity of the measured temperature compared to the estimated temperature of red pepper, soybean and persimmon was 1.078, 1.033 and 0.973, respectively.

Molecular Cloning and Expression of Genes Related to Antifungal Activities from Enterobacter sp. B54 Antagonistic to Phytophthora capsici

  • YOON, SANG-HONG
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.352-357
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    • 1999
  • Enterobacter sp. B54 inhibited growth of the fungus Phytophthora capsici on potato dextrose agar (PDA). Three mutants with antifungal activities (denoted M54-47, M54-113, and M54-329) which were lost or increased, through Pl::Tn5 lac mutagenesis, were used to isolate genes responsible for fungal inhibition on PDA. Two clones were selected from the partially EcoR1-digested genomic library of the wild-type strain by probing with genomic flanking sequences of each mutant. We have isolated a 20-kb EcoR1 genomic DNA fragment from this strain that contains genes involved in hyphal growth inhibition of P. capsici on PDA. Subcloning and expression analysis of the above DNA fragment identified a 8-kb region which was necessary for antifungal activities. A 8-kb HindⅢDNA fragment covers three genomic loci inserted by Tn5 lac in each mutant. This suggested that all genes which are related to antifungal activities might be clustered in simple forms of at least 5-8 kb sizes.

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A Study of the Level of Cardiac Troponin I in Patients with Clinically Suspected Acute Myocarditis Treated with Intravenous Gammaglobulin (심근형 Troponin I의 증가로 급성 심근염의 진단을 받은 환아에서 정맥용 면역글로부린 치료 효과에 대한 연구)

  • Kim, Minshik;Lee, Youngok;Chun, Yoonae
    • Pediatric Infection and Vaccine
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    • v.5 no.2
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    • pp.267-275
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    • 1998
  • Purpose : We have studied the changes of cardiac troponin I(cTnI) level and left ventricular ejection fraction(LVEF) before and after treatment of IVGG to evaluate the efficacy of single high dose of intravenous gammaglobulin(IVGG)(2.0gm/kg) therapy for improving cardiac function and clinical symptoms and signs in patients with clinically suspected acute myocarditis. Methods : The patients consisted of 18 cases who admitted increased cTnI with clinically suspected acute myocarditis caused by viral infection, Kawasaki disease and fever unknown origin(FUO) from Jan. 1995 to Jun. 1998. The control group consisted of 20 cases suffered from hand-foot-mouth disease, herpangina and high fever with rash. The level of cTnI was measured by Chemiluminiscent immunoassay method(normal<0.1ng/ml) and cardiac function was evaluated by left ventricular ejection fraction(LVEF)(normal 64~83%) by echocardiogram. Results : The level of cTnI increased to $0.306{\pm}0.209ng/ml$ and LVEF decreased to $60.1{\pm}1.6%$ before treatment of IVGG significantly as compared with control group(P<0.05). All cases were returned to normal range of LVEF($71.4{\pm}3.7%$) and decreased cTnI significantly($0.089{\pm}0.082ng/ml$) after treated with IVGG within 1 week in patients group(P<0.05). Conclusion : The single high dose of IVGG(2.0gm/kg) therapy was rapid and effective improvement of cardiac function and clinical symptoms and signs of acute myocarditis, and the measurement of serum cTnI and LVEF may help to diagnose and evaluate efficacy of IVGG on it.

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Pressure Effect on the Aquation of trans-[Cr(tn)$_2Cl_2]^+$ and trans-[Cr(en)(tn)Cl$_2]^+$ Complex Ions (trans-[Cr(tn)$_2Cl_2]^+$ 및 trans-[Cr(en)(tn)Cl$_2]^+$ 착이온의 수화반응에 미치는 압력효과)

  • Jong-Jae Chung;Jong-Ha Choi;Deog-Jin Kim
    • Journal of the Korean Chemical Society
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    • v.31 no.5
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    • pp.375-381
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    • 1987
  • Rates for the aquation of trans-[Cr(tn)$_2Cl_2]^+$ and trans-[Cr(en)(tn)Cl$_2$]^+$ ions in aqueous acidic solution have been measured by spectrophotometric method at various temperatures and pressures. Activation volumes are negative and lie in the limited range -1.7 ∼ -2.9cm$^3$mol$^{-1}$ or the complex ions. Activation entropies and activation compressibility coefficients are small negative values. From the results of thermodynamic parameters, it can be inferred that the aquation of the complex ions proceed through an associative interchange(Ia) mechanism. Furthermore, the information on possible transition state structure and reaction paths can be obtained by considering total stabilization energy of the hypothetical intermediates within the framework of angular overlap model. It is found that the theoretically predicted mechanism is consistent with the experimentally observed results.

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The Nitrogen Behavior and Budget in Lake Paldang (팔당호의 질소거동과 수지)

  • Lee, Jangho;Park, Hae-Kyung;Lee, Kyoo;Kim, Eunmi
    • Journal of Korean Society on Water Environment
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    • v.26 no.1
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    • pp.71-80
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    • 2010
  • We studied the nitrogen behavior and budget of Lake Paldang from March to December 2008. The particulate nitrogen (PN) concentrations ranged from 7 to 13% of the total nitrogen concentration (TN) in the stream inflows, the downstream outflow, and the lake water. The nitrate nitrogen ($NO_3-N$) concentration ranged from 67 to 78% of the TN. In the three rivers of Lake Paldang, Gyeongan River (In3 site) had the highest average of the TN, 5.037 mgN/L, but North Han River (In2 site) had the lowest average TN, 1.683 mgN/L. South Han River (In1 site) had the average TN of 2.399 mgN/L. In the dam discharge, TN showed the average 2.063 mgN/L. In the lake water, L4 site (Gyeongan River area) had the highest average TN, 3.781 mgN/L, but L3 site (North Han River) had the lowest average TN, 1.587 mgN/L. Total input of nitrogen loads to Lake Paldang was about 30,875 ton/year in 2008. Inflow rivers contributed 30,643 ton/year (South Han River: 18,111 ton/year (59%), North Han River: 11,333 ton/year (37%), and Gyeongan River: 1,199 ton/year (4%)). The atmospheric deposition had 135 ton/year, the nitrogen release from the bottom sediments had 88 ton/year, and macrophytes had 9 ton/year. Total output of nitrogen loads from Lake Paldang was about 31,256 ton/year. The downstream from dam contributed 29,877 ton/year, and the sediment deposition was 1,379 ton/year.

Comparison of Seasonal Nitrogen Removal by Free-Water Surface Wetlands Planted with Iris pseudacorus L. (노랑꽃창포 자유수면습지의 계절에 따른 질소제거 비교)

  • Yang, Hongmo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.14 no.1
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    • pp.121-132
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    • 2011
  • Removal rates of $NO_3$-N and TN in the free-water surface wetland system during winter; December, January, February and March, spring and fall; April, May, October and November, and summer; Jun, July, August and September were investigated. The system was established on floodplain in the downstream reach of the Gwangju Stream in 2008. It measures 50 meters in length and 5.5 meters in width. Iris pseudacorus L. grown in pots for about two years were planted in the system. The water stream was funneled in by gravity and its effluent was discharged back in. Volumes and water quality of inflow and outflow were analyzed from December 2008 to November 2010. The inflow was averaged approximately 350 $m^3/day$ and hydraulic residence time was about 3 hours. Average influent and effluent $NO_3$-N concentration was 3.75 and 3.35 mg/L, respectively and $NO_3$-N retention was amounted to 10.6%. Influent and effluent TN concentration were averaged 4.93 and 4.30 mg/L, respectively and TN abatement reached to 12.9%. One-way ANOVA statistics claimed that the average removal rates of $NO_3$-N and TN during winter, spring and fall, and summer were not always the same (p<0.001). The t-Tests of three pairs among $NO_3$-N removal rates of winter, spring and fall, and summer illustrated that the removal rates of winter ($5.04{\pm}1.94$), spring and fall ($10.53{\pm}2.24$), and summer ($18.61{\pm}2.26$) were significantly different each others (p<0.001). Among TN removal rates, the three pairs of t-Tests of three seasons showed that the removal rates of winter ($5.21{\pm}2.51$), spring and fall ($11.71{\pm}3.12$), and summer ($21.53{\pm}4.86$) were significantly different from each others (p<0.001).

Availability of Cardiac Troponin T as a Marker for Detecting Perioperative Myocardial Damage in Patients with Open Heart Surgery (개심술후 발생한 심근손상에서 진단 표지자로서의 Troponin T의 임상적 유용성)

  • 김태이;정태은;이동협;이정철;한승세
    • Journal of Chest Surgery
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    • v.31 no.1
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    • pp.20-27
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    • 1998
  • It is well known that troponin T(below TnT) is present in the myocardial cells and released during myocardial damage, so it`s very specific enzyme to myocardium. Availability of cardiac specific TnT in assessing perioperatively myocardial damage was evaluated from 34 open heart surgery patients. They consisted of 11 ischemic heart, 13 acquired valvular heart and 10 congenital heart cases. Patients were divided into two groups, group A(patients with myocardial damage) and group B(patients without myocardial damage), according to the symptom of chest pain suspecting angina and the ECG findings of ST segment and T wave changes which show myocardial ischemia and injury. Serum TnT levels were measured by enzyme immunoassay method preoperatively, immediately postoperatively, postoperative day 1, day 2, day 3, and day 7. We observed and analyzed the changes of serum TnT levels in two groups and compared the serum TnT levels with CK-MB levels measured at the same time. In group A, serum TnT levels showed 1.37$\pm$0.26$\mu$g/L, 3.16$\pm$0.66$\mu$g/L, 2.39$\pm$0.74$\mu$g/L, 2.49$\pm$0.76$\mu$g/L, and 1.23$\pm$0.60$\mu$g/L, immediate postoperatively, postoperatively day1, day2, day3, and day7, respectively. It was observed there were significant differences compared with those of group B(0.38$\pm$0.04$\mu$g/L, 0.34$\pm$0.05$\mu$g/L, 0.25$\pm$0.03$\mu$g/L, 0.24$\pm$0.04$\mu$g/L, and 0.11$\pm$0.03$\mu$g/L) during identical periods(P<0.01). Serum CK-MB level in group A significantly elevated to 145.04$\pm$35.08 IU/L on the postoperative day 1 compared to group B(31.28$\pm$5.87 IU/L, P<0.05), However, it stiffly decreased from day 2 and returned to preoperative level at day 3. When serum TnT level more than 1.0$\mu$g/L is thought to reflect myocardial damage, serum TnT had 100% of sensitivity and 87% of specificity in diagnosing the postoperative myocardial damage(p<0.01). I conclusion, serum TnT levels increased significantly at very early stage of myocardial damage and persisted much longer period than CK-MB. This suggests that serum TnT has more advantage and availability in assessing the perioperatively myocardial damage than any other tests.

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Changes in Air Temperature and Surface Temperature of Crop Leaf and Soil (기온과 작물 잎 및 토양 표면온도의 변화양상 분석)

  • Lee, Byung-Kook;Jung, Pil-Kyun;Lee, Woo-Kyun;Lim, Chul-Hee;Eom, Ki-Cheol
    • Journal of Climate Change Research
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    • v.6 no.3
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    • pp.209-221
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    • 2015
  • Temperature is one of the most important factors affecting crop growth. The diurnal cycle of the scale factor [Tsc] for air temperature and the surface temperature of crop leaf and soil could be estimated by the following equation : $[Tsc]=0.5{\times}sin(X+C)+0.5$. The daily air temperature (E[Ti]) according to the E&E time [X] can be estimated by following equation using average (Tavg), maximum (Tm) and minimum (Tn) temperature : $E[Ti]=Tn+(Tm-Tn){\times}[0.5{\times}sin\;\{X+(9.646Tavg+703.65)\}+0.5]$. The crop leaf temperature in 24th June 2014 was high as the order of red pepper without mulching > red pepper with mulching > soybean under drought > soybean with irrigation > Chinese cabbage. The case in estimating crop leaf surface temperature using air temperature and soil surface temperature was lower in the deviation compared to the case using air temperature for Chinese cabbage and red pepper. These results can be utilized for the crop models as input data with estimation.

Study on Introduction to Predicting Indicator of Cyanobacteria Dominance in Algae Bloom Warning System of Hangang Basin (한강유역 조류경보제에 남조류 우점 예측인자 도입에 관한 연구)

  • Kim, Tae Kyun;Choi, Jae Ho;Lee, Kyung Ju;Kim, Young Bae;Yu, Sung Jong
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.5
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    • pp.378-385
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    • 2014
  • The chlorophyll-a concentration in algae bloom warning system of Hangang basin did not predict the cyanobacteria dominance. In this study, suggest the predicting indicator of cyanobacteria dominance through analyzing the environmental factors affecting on the cell count of cyanobacteria. Firstly, the dominance of algae was analyzed with seasonal variation during Jan. 2012~Sep. 2013. The diatom dominated phytoplankton communities during the period of January~April. In the May~June, the green algae dominated. And, the dominance of algae was changed to cyanobacteria in the July~August. Also, the environmental factors affecting to cyanobacteria blooms ; nutrients (TN, TP), temperature, precipitation, dam-discharge were evaluated during the study period. Rather than temperature factor, relatively low dam discharge causes cyanobacteria to grow rapidly and create a blooms. The low dam-discharge may increase the water retention time. Finally, it is proved that a low ratio of TN to TP (<29:1) can favour the development of cyanobacteria blooms. Thus, the predicting indicator (TN:TP) have need to apply to the alarm bloom warning system of Hangang basin.

Molecular Cloning of Antagonistic Genes in Pseudomonas maItophiliQ B-14 (토양병해 길항성 Pseudomonas maltophilia B-14의 길항유전자탐색)

  • 구본성;서영우;윤상홍;박경수;은무영;김용환;오상우;류진창;은무영
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.619-624
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    • 1992
  • Tn5 lac 삽입으로 채소입고병원균에 길항력이 약화된 T-67 및 고추역병균과 참깨역병균에 길항력이 약화된 T-81의 Tn5 lac 유전자 일부와 오른쪽 말단에 있는 길항관련 유전자의 flanking sequence가 cloning된 pAG67 및 pAG81 clone을 선발하였고, pAG67 및 pAG81 clone된 길항관련 유전자의 flanking sequence를 야생 길항균 Pseudomonas maltophilia B-14의 DNA를 probe로 사용하여 Southern hybridization으로 확인하였으며, 제한효소 지도를 작성하여 8Kb 및 4Kb 크기의 flanking sequence가 cloning되었음을 확인하였다. pAG6 및 pAG81의 flanking sequence를 EcoRi-BglII와 EcoRI-MpaI으로 분리하여 유전자 은행으로부터 길항관련 유전자가 cloning된 cosmid clone 7개주를 선발하였다.

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