• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1422-1428
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• 2006

An ABC transporter apparatus of the Gram-negative bacterial type I secretion pathway can be used as a secretory protein expression system in Escherichia coli. Four types of coexpression systems for the Pseudomonas fluorescens lipase gene, tliA, and its cognate ABC transporter gene cluster, tliDEF, were constructed. When the relative expression levels were changed by adding different concentrations of IPTG, the secretion (16.9 U/ml of culture) of TliA in E. coli [pTliDEFA-223+pACYC184] was significantly higher than E. coli [pKK223-3+pTliDEFA-184] secreting the lowest level of TliA (5.2 U/ml of culture). Maximal accumulation of the lipase secreted occurred in the mid-exponential phase, implying that the efficient protein secretion via an ABC transporter was restricted only to actively growing cells. Finally, the secretion level of TliA in E. coli [pTliDEFA-223+pACYC184] was increased to 26.4 U/ml by inducing gene expression at the culture initiation time. These results indicate that a significant increase in the ABC transporter-dependent protein secretion can be achieved by simply controlling the relative expression levels between the ABC transporter and its passenger protein, even in the recombinant E. coli cells.

• Bulletin of the Korean Space Science Society
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• 2009.10a
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• pp.40.2-40.2
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• 2009

이 연구에서는 달천이(TLI: Trans Lunar Injection) 및 달포획(LOI: Lunar Orbit Injection) 기동 시 대전 지상국의 가시성을 고려한 최적의 임무를 설계하였다. TLI 기동은 탐사선이 지구 주차궤도에서 지구-달 천이궤적으로 진입하기 위하여 주어지는 기동이며, LOI 기동은 탐사선이 지구-달 천이궤적에서 달의 중력권으로 진입하기 위하여 주어지는 기동이다. TLI 및 LOI 기동 시 대전 지상국에서의 가시성의 확보는 실제적인 미래 한국의 달 탐사를 대비하였을 때 중요한 요소이다. 따라서 이 연구에서는 TLI 및 LOI 기동 시 대전 지상국에서의 가시성을 모두 고려하여, 최소연료로 지구 주차궤도에서 달 임무궤도 진입까지의 모든 단계에 대해 임무설계를 실시하였다. TLI 및 LOI 기동 시 추력은 순간 추력(Impulsive thrust)로 가정하였으며, KSLV-II 발사체의 성능을 적용하여 설계하였다. 임무 설계 시 태양, 지구, 달의 섭동력을 고려한 N체 운동 방정식을 탐사선에 적용하였으며, 지구의 비대칭 중력장, 태양 복사압, 달의 J2 섭동에 의한 영향도 고려하였다. JPL의 정밀 천체력인 DE405를 사용하였고, 상용 소프트웨어인 SNOPT(Spares Nonlinear OPTimizer)를 이용하여 비행 궤적의 최적해를 도출하였다. 임무 설계 결과를 통해, 대전 지상국의 가시성을 고려한 TLI 및 LOI 기동의 크기에 의한 임무설계의 분석을 수행하였다. 또한 최적화된 달 탐사 임무의 단계별 기동의 크기와 지구-달 천이 궤적의 형상 및 다양한 임무 요소들의 해석을 도출하였다.

• Journal of Astronomy and Space Sciences
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• v.27 no.3
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• pp.195-204
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• 2010

The optimal Earth-Moon transfer trajectory considering spacecraft's visibility from the Daejeon ground station visibility at both the trans lunar injection (TLI) and lunar orbit insertion (LOI) maneuvers is designed. Both the TLI and LOI maneuvers are assumed to be impulsive thrust. As the successful execution of the TLI and LOI maneuvers are crucial factors among the various lunar mission parameters, it is necessary to design an optimal lunar transfer trajectory which guarantees the visibility from a specified ground station while executing these maneuvers. The optimal Earth-Moon transfer trajectory is simulated by modifying the Korean Lunar Mission Design Software using Impulsive high Thrust Engine (KLMDS-ITE) which is developed in previous studies. Four different mission scenarios are established and simulated to analyze the effects of the spacecraft's visibility considerations at the TLI and LOI maneuvers. As a result, it is found that the optimal Earth-Moon transfer trajectory, guaranteeing the spacecraft's visibility from Daejeon ground station at both the TLI and LOI maneuvers, can be designed with slight changes in total amount of delta-Vs. About 1% difference is observed with the optimal trajectory when none of the visibility condition is guaranteed, and about 0.04% with the visibility condition is only guaranteed at the time of TLI maneuver. The spacecraft's mass which can delivered to the Moon, when both visibility conditions are secured is shown to be about 534 kg with assumptions of KSLV-2's on-orbit mass about 2.6 tons. To minimize total mission delta-Vs, it is strongly recommended that visibility conditions at both the TLI and LOI maneuvers should be simultaneously implemented to the trajectory optimization algorithm.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.2
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• pp.351-359
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• 2010

In this paper, we propose a transmission algorithm of the TLI protocol of the IEC 61162-4 standard to perform the e-Navigation on ship, and we design and implement the TLI protocol according to the proposed algorithm. The implemented protocol is a TCP/IP based protocol, and is a network protocol with the MAU and LNA components proposed in the IEC 61162-4 standard. In addition, it makes a data transmission over the network architecture based on client/server network model. In the implemented protocol, there are a communication between the MAU and the LNA in one host, and also a communication between the LNA and the LNA in each other hosts. In order to provide a variety of service in a host, every client host has a LNA and multiple MAUs. According to the standard specification and the proposed transmission algorithm, we designed and implemented the TLI protocol using object-oriented mechanism. We carried out the experiment under a simple network model similar to real ship environment, and confirmed that it successfully transmits and receives data between the hosts.

• Journal of Veterinary Clinics
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• v.18 no.1
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• pp.7-13
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• 2001

Depending on the degree of exocrine pancreatic insufficiency, there are inconsistent values in the serum trypsin-like immunoreactivity (TLI), hematology, and serology. To determine the correlation between those values and complete pancreatic insufficiency, 10 pancreatectomized dogs as the treated group and 10 sham operation dogs as control group were used. The entire treated group showed significant decrease in serum TLI level from 2.63$\pm$0.20 ng/ml (day 0) to 1.81$\pm$0.43 ng/ml at day 1 after the pancreatectomy (p<0.05) and the decline maintained till the end of the experiment. Blood glucose level gradually increased, but albumin level showed significant decrease (p<0.05) at day 1 and maintained the decline. Three clinical signs were observed such as depression, vomiting, and diarrhea. The entire treated group showed diarrhea throughout the experiment, but depression and vomiting were gradually diminished. The activity of serum alkaline phosphatase in the treated group increased till day 2 then decreased, on the other hand, control group showed continuous increase till day 4, and then decreased. Only experimental group showed the increased alanine aminotransferase activity at day 1. Serum lipase activity in the treated group jumped up at day 1 and then dropped down, which was even lower than the control. Regarding serum amlyase activity gradually decreased. According to these results, the assay of trypsin-like immunoreactivity showed the consistent result, so it suggests that TLI is an useful tool to determine the pancreatic exocrine function and possible diagnosis of exocrine pancreatic insufficiency compared to the simple assay of plasma amylase and lipase.

• KSBB Journal
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• v.24 no.4
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• pp.393-396
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• 2009

New protein secretion system was developed using Type III Secretion System of Salmonella. N-terminal region of SlrP and SptP effector proteins were fused with TliA and EstA-P lipases by overlapping PCR. Lipase activity of Salmonella with SptP-TliA fusion system increased by 2.6 fold compare with wild type Salmonella strain. This result showed that lipase secretion via the T3SS would be a useful protein secretion machinery.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.317-323
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• 2008

Canine trypsin-like immunoreactivity (cTLI), which is a mirror of the concentration of trypsin and trypsinogen, is a pancreas-specific enzyme and a suitable marker for canine pancreatitis and especially exocrine pancreatic insufficiency (EPI). To develop the immunochromatographic test kit, monoclonal antibodies that recognize cTLI were prepared. Anionic trypsin, cationic trypsin, and chymotrypsin from canine pancreas were successfully purified to homogeneity, using ammonium sulfate fractionation and benzamidine-affinity chromatography. The purification fold for anionic trypsin was 108 times when compared with that of the homogenation of pancreas. The molecular weights by SDS-PAGE analysis were approximately 23 kDa for chymotrypsin and approximately 20 kDa for cationic trypsin and anionic trypsin, respectively. Using the purified trypsin-like proteins, ten hybridomas which secret canine trypsin-specific monoclonal antibody were prepared. Klotz plot indicated that hybridomas, 5G2H10G4 and 2F4A11, have high affinity constant (Ka) of $4.1\;{\times}\;10^{9}$ and $1.8\;{\times}\;10^{9}$, respectively. Especially, 5F9H3 showed the cationic typsin-specific binding pattern and its Ka was determined to $4.5\;{\times}\;10^{9}$. The development of immunochromatographic test kit using these monoclonal antibodies against cTLI will be very useful in the diagnosis of canine EPI or canine pancreatitis.

• Korean Chemical Engineering Research
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• v.58 no.2
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• pp.280-285
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• 2020

We have developed a constitutive display system of the Pseudomonas fluorescens SIK W1 TliA lipase on the cell surface of Escherichia coli using E. coli outer membrane protein C (OmpC) as an anchoring motif, which is an economical compared to induced system. For the constitutive expression of truncated OmpC-TliA fusion proteins, gntT104 promoter was employed. Cell growth was not affected by over expression of fusion protein during entire culture time, suggesting cell lysis was not a problem. The localization of truncated OmpC-TliA fusion protein on the cell surface was confirmed by immunofluorescence microscopy and measuring whole cell lipase activity. Constitutively displayed lipase was very stable, retaining activity enantioselectivity throughout the five repeated reactions. These results suggest that OmpC from E. coli be a useful anchoring motif for displaying enzymes on the cell surface without any inducers, and this stable surface display system can be employed for a broad range of biotechnological applications.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.42 no.8
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• pp.654-660
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• 2014

In this paper, a conceptual design of a launch vehicles is proposed by combining Naro-1 and KSLV-II. For trans-lunar injection (TLI) to lunar orbit at 300 km LEO, the target performance is defined same as that of KSLV-II, which delivers an object of 2.6 tons into 300 km LEO. The proposal launch vehicle concept of this study is combination of 1st stage of KSLV-I and 2-3rd stage of KSLV-II. Thus, it is possible to reduce the development time and also could expand the options for national launch vehicle capabilities with proven technologies.

• Journal of the Korea Society for Simulation
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• v.30 no.2
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• pp.33-40
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• 2021

The situation of high-altitude electromagnetic pulses (HEMP) arises from high-altitude nuclear explosions. The HEMP situation can be simulated through the threat level investigation (TLI). In this paper, the induced voltage according to the antenna type of the tactical mobile radio communication system was measured and analyzed by TLI. Under the influence of HEMP, electronic equipment can be paralyzed or damaged. HEMP protection filters are commercially available for power lines and signal lines. However, commercialization of HEMP filters for antennas is insufficient, and even some of them exist for lightning protection. In order to make an appropriate HEMP protection filter according to the frequency and type of the antenna, the induced voltage was measured and the maximum induced voltage was analyzed through extrapolation. It was found that the measured induced voltage decreased as the frequency increased, such as in the HF, VHF and UHF bands of the measurement results.