• The Journal of Korean Academy of Prosthodontics
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• v.42 no.3
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• pp.261-266
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• 2004

Statement of problem. The effects of surface roughness have not or insufficiently been analyzed on earlier events such as cell adhesion though cell behavior most germane to implant performance is cell adhesion. Purpose. The purpose of this study was to evaluate cell adhesion of osteoblast-like cells (MG63) onto three types of titanium disks with varying roughness using the Elisa assay. Materials and methods. Representative disks from each group (SLA, HA, machined) were subjected to surface analysis and surface roughness was measured by the optical interferometer (Accura 2000, Intekplus Co., Seoul, Korea). Following this, MG63 cells were cultured on the titanium disks and released. Cell adhesion measurements using the Elisa assay were performed specifically at three points: after 24, 48, and 72 hours of culture. Results. Among the 3 types of surface analyzed, the SLA surface was the roughest with a Ra value of $1.114{\mu}m$ followed by HA coated surface and machined surface, consecutively. The optical density values for the SLA surface group was significantly higher than that of the machined and HA coated surface groups following 24 and 48 hours of culture. The cell culture on HA coated surface showed significantly higher values compared to the machined surface following 24, 48 and 72 hours of culture. Conclusion. The results suggest that surface treatment of titanium surfaces enhanced cell adhesion of human osteoblast-like cells (MG63).

• Journal of the Korean Ceramic Society
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• v.45 no.11
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• pp.667-674
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• 2008

In this study, we prepared Fe-activated carbon fiber(ACF)/$TiO_2$ composites with titanium (VI) n-butoxide (TNB) as the titanium source for ACF pre-treated with iron compounds through the impregnation method. In terms of textural surface properties, the composites demonstrate a slight decrease in the BET surface area of the samples and an increase in the amount of iron compounds treated. The surface morphology of the Fe-ACF/$TiO_2$ composites was characterized by means of SEM. The composites have a porous texture with homogenous compositions of Fe and titanium dioxide distributed on the sample surfaces. The phase formation and structural transition of the iron compounds and titanium dioxide were observed in X-ray diffraction patterns of the Fe-ACF/$TiO_2$ composites. The chemical composition of the Fe-ACF/$TiO_2$ composites, which was investigated with EDX shows strong peaks for the C, O, Fe and Ti elements. The photo degradation results confirm that the Fe-ACF/$TiO_2$ composites show excellent removal activity for the COD in piggery waste due to photocatalysis of the supported $TiO_2$, radical reaction by Fe species, and the adsorptivity and absorptivity of ACF.

• Journal of Korea Foundry Society
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• v.37 no.4
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• pp.115-122
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• 2017

The aim of this research is to evaluate the wear properties of (TiB+TiC) paticulate reinforced titanium matrix composites (TMCs) by in-situ synthesis. Different particle sizes (1500, $150{\mu}m$) and contents (0.94, 1.88 and 3.76 mass% for Ti, 1.98 and 3.96 mass% for the Ti6Al4V alloy) of boron carbide were added to pure titanium and to a Ti6Al4V alloy matrix during vacuum induction melting to provide 5, 10 and 20 vol.% (TiB+TiC) particulate reinforcement amounts. The wear behavior of the (TiB+TiC) particulate reinforced TMCs is described in detail with regard to the coefficient of friction, the hardness, and the degree of reinforcement fragmentation during sliding wear. The worn surfaces of each sliding wear condition are shown for the three types of wear studied here: transfer layer wear, particle cohesion wear and the development of abrasive areas. The fine reinforcements of TMCs were easily fragmented from the Ti matrix as compared to coarse reinforcements, and fragmented debris accelerated the decrease in the wear resistance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.4 no.1
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• pp.13-16
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• 2003

We implanted $N^+ion$ into TiO$_2$/Ti substrates with 70 keV by varying dose of 0, 2, 5, and $10{\times}10^{17}/cm$^2$$. In addition, $N^+ion$implanted TiO$_2$ specimens were annealed at $600^{\circ}C$ for 2 hours in Atmosphere. We investigated the color evolution, surface roughness, and hardness of specimens with doses. We report that the color changed from white into dark-yellow as dose increased. ion implanted surfaces became smooth when they were annealed. Moreover, hardness increased up to 10% when we annealed ion implanted TiO$_2$. Our results imply that we may enhanced titanium color and surface hardness.

• Applied Science and Convergence Technology
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• v.25 no.3
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• pp.51-55
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• 2016

The surface treatment of a titanium implant is investigated with a non-thermal atmospheric pressure plasma jet. The plasma jet is generated by the injection of He and $O_2$ gas mixture with a sinusoidal driving voltage of 3 kV or more and with a driving frequency of 20 kHz. The generated plasma plume has a length up to 35 mm from the jet outlet. The wettability of 4 different titanium surfaces with plasma treatments was measured by the contact angle analysis. The water contact angles were significantly reduced especially for $O_2/He$ mixture plasma, which was explained with the optical emission spectroscopy. Consequently, plasma treatment enhances wettability of the titanium surface significantly within the operation time of tens of seconds, which is practically helpful for tooth implantation.

• The Journal of Korean Academy of Prosthodontics
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• v.38 no.2
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• pp.200-213
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• 2000

Lasers have given dentistry a new rapid, economic, and accurate technique for metal joining. Although laser welding has been recommended as an accurate technique, there are some limitations with this technique. For example, the two joining surfaces must have a tight-fitting contact, which may be difficult to achieve in some situations. The tensile samples used for this study were made from a custom-made pure titanium and type III gold alloy plates. 27 of 33 specimens were sectioned perpendicular to their long axis with a carborundum disk and water coolant. Six specimens remained and served as the control group. A group of 6 specimens was posed as butt joints in custom parallel positioning device with a feeler gauge at each of three gaps : 0.00, 0.25. and 0.50mm. All specimens were then machined to produce a uniform cross-sectional dimension, none of the specimens was subjected to any subsequent form of heat treatment. Scanning electron microscopy was performed on representative tested specimens at fractured surfaces in both the parent metal and the weld. Vickers hardness was measured at the center of the welds with a micropenetrometer using a force of 300gm for 15 seconds. Measurement was made at approximately $200{\mu}m\;and\;500{\mu}m$ deep from each surface. One-way analysis of variance (ANOVA) and Scheffe's test was calculated to detect differences between groups. The purpose of this study is to compare the strength and properties of the joint achieved at various butt Joint gaps by the laser welding of type III gold alloy and pure titanium tensile specimens in an argon atmosphere. The results of this study were as follows : 1. When indexing and welding pure titanium, there was no decrease in ultimate tensile strength as compared with the unsectioned alloys for indexing gaps of 0.00 to 0.50mm, although with increasing gap size may come increased distortion (p>0.05). 2. When indexing and welding type III gold alloy, there were significant differences in ultimate tensile strength among groups with weld gaps of 0.00mm, 0.25 and 0.50mm, and the control group. Group with butt contact without weld gap demonstrated a significant higher ultimate tensile strength than groups with weld gaps of 0.25 and 0.50mm (p<0.05). 3. When indexing and welding the different metal combination of type III gold alloy and pure titanium, there were significant differences in ultimate tensile strength between groups with weld gaps of 0.00, 0.25, and 0.50mm. However, the mechanical properties of the welded joint would become too brittle to be acceptable clinically (p<0.05). 4. The presence of large pores in the laser welded joint appears to be the most important factor in controlling the tensile strength of the weld in both pure titanium and type III gold alloy.

• The Journal of Korean Academy of Prosthodontics
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• v.43 no.3
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• pp.363-378
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• 2005

Statement of problem. Hydroxyapatite(HA) coated titanium surfaces have not yet showed the reliable osseointegration in various conditions. Purpose. This study was aimed to investigate microstructures, chemical composition, and surface roughness of the surface coated by the hydrothermal method and to evaluate the effect of hydrothermal coating on the cell attachment, as well as cell proliferation. Material and Methods. Commercially pure(c.p.) titanium discs were used as substrates. The HA coating on c.p. titanium discs by hydrothermal method was performed in 0.12M HCl solution mixed with HA(group I) and 0.1M NaOH solution mixed with HA(group II). GroupⅠ was heated at 180 $^{\circ}C$ for 24, 48, and 72 hours. GroupⅡ was heated at 180 $^{\circ}C$ for 12, 24, and 36 hours. And the treated surfaces were evaluated by Scanning electron microscopy(SEM), Energy dispersive X-ray spectroscopy(EDS), X-ray photoelectron spectroscopy(XPS), X-ray diffraction method(XRD), Confocal laser scanning microscopy(CLSM). And SEM of fibroblast and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay were used for cellular responses of the treated surfaces. Results. The color of surface changed in both groups after the hydrothermal process. SEM images showed that coating pattern was homogeneous in group II, while inhomogeneous in group I. H72 had rosette-like precipitates. The crystalline structure grew gradually in group II, according to extending treatment period. The long needle-like crystals were prominent in N36. Calcium(Ca) and phosphorus(P) were not detected in H24 and H48 in EDS. In all specimens of group II and H72, Ca was found. Ca and P were identified in all treated groups through the analysis of XPS, but they were amorphous. Surface roughness did not increase in both groups after hydrothermal treatment. The values of surface roughness were not significantly different between groups I and II. According to the SEM images of fibroblasts, cell attachments were oriented and spread well in both treated groups, while they were not in the control group. However, no substantial amount of difference was found between groups I and II. Conclusions. In this study during the hydrothermal process procedure, coating characteristics, including the HA precipitates, crystal growth, and crystalline phases, were more satisfactory in NaOH treated group than in HCl treated group. Still, the biological responses of the modified surface by this method were not fully understood for the two tested groups did not differ significantly. Therefore, more continuous research on the relationship between the surface features and cellular responses seems to be in need.

• The Journal of Korean Academy of Prosthodontics
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• v.42 no.3
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• pp.307-326
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• 2004

Statement of problem. The long-term success of implants is the development of a stable direct connection between bone and implant surface, which must be structural and functional. To improve a direct implant fixation to the bone, various strategies have been developed focusing on the surface of materials. Among them, altering the surface properties can modify cellular responses such as cell adhesion, cell motility and bone deposition. Purpose. This study was to evaluate the cellular behaviors on the surface-modified titanium by morphological observation, cellular proliferation and differentiation. Material and methods. Specimens were divided into five groups, depending on their surface treatment: electropolishing(EP) anoclizing(AN), machining(MA), blasting with hydroxyapatite particle(RBM) and electrical discharge machining(EDM). Physicochemical properties and microstructures of the specimens were examined and the responses of osteoblast-like cells were investigated. The microtopography of specimens was observed by scanning electron microscopy(SEM). Surface roughness was measured by a three-dimensional roughness measuring system. The microstructure was analyzed by X-ray diffractometer(XRD) and scanning auger electron microscopy(AES). To evaluate cellular responses to modified titanium surfaces, osteoblasts isolated from neonatal rat were cultured. The cellular morphology and total protein amounts of osteoblast-like cell were taken as the marker for cellular proliferation, while the expression of alkaline phosphatase was used as the early differentiation marker for osteoblast. In addition, the type I collagen production was determined to be a reliable indicator of bone matrix synthesis. Results. 1. Each prepared specimen showed specific microtopography at SEM examination. The RBM group had a rough and irregular pattern with reticulated appearance. The EDM-treated surface had evident cracks and was heterogeneous consisting of broad sheet or plate with smooth edges and clusters of small grains, deep pores or craters. 2. Surface roughness values were, from the lowest to the highest, electropolished group, anodized group, machined group, RBM group and EDM group. 3. All groups showed amorphous structures. Especially anodized group was found to have increased surface oxide thickness and EDM group had titaniumcarbide(TiC) structure. 4. Cells on electropolished, anodized and machined surfaces developed flattened cell shape and cells on RBM appeared spherical and EDM showed both. After 14 days, the cells cultured from all groups were formed to be confluent and exhibited multilayer proliferation, often overlapped or stratified. 5. Total protein amounts were formed to be quite similar among all the group at 48 hours. At 14 days, the electropolished group and the anodized group induced more total protein amount than the RBM group(P<.05). 6. There was no significant difference among five groups for alkaline phosphatase(ALP) activity at 48 hours. The AN group showed significantly higher ALP activity than any other groups at 14 days(P<.05). 7. All the groups showed similar collagen synthesis except the EDM group. The amount of collagen on the electropolished and anodized surfaces were higher than that on the EDM surface(P<.05).

• The Journal of Korean Academy of Prosthodontics
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• v.41 no.3
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• pp.300-318
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• 2003

Statement of problem: The success of implants depends on intimate and direct contact of implant material on bone tissue and on functional relationship with soft tissue contact. Creation and maintenance of osseointegration depend on the understanding of the tissue's healing, repairing, and remodeling capacity and these capacities rely on cellular behavior. Altering the surface properties can modify cellular responses such as cell adhesion, cell motility, bone deposition, Therefore, various implant surface treatment methods are being developed for the improved bone cell responses. Purpose: The purpose of this study was to evaluate the responses of osteoblast-like cells to surface-modified titanium. Materials and Methods: The experiment was composed of four groups. Group 1 represented the electropolished surface. Group 2 surfaces were machined surface. Group 3 and Group 4 were anodized surfaces. Group 3 had low roughness and Group 4 had high roughness. Physicochemical properties and microstructures of the discs were examined and the responses of osteoblast-like cells to the discs were investigated. The microtopography was observed by SEM. The roughness was measured by three-dimension roughness measuring system. The microstructure was analyzed by XRD, AES. To evaluate cell responses to modified titanium surfaces, osteoblasts isolated from calvaria of neonatal rat were cultured. Cell count, morphology, total protein measurement and alkaline phosphatase activities of the cultures were examined. Results and Conclusion: The results were as follows 1. The four groups showed specific microtopography respectively. Anodized group showed grain structure with micropores. 2. Surface roughness values were, from the lowest to the highest, electropolished group, machined group, low roughness anodized group, and high roughness anodized group. 3. Highly roughened anodized group was found to have increased surface oxide thickness and surface crystallinity. 4. The morphology of cells, flattened or spherical, were different from each other. In the electropolished group and machined group, the cells were almost flattened. In two anodized groups, some cells were spherical and other cells were flattened. And the 14 day culture cells of all of the groups were nearly flattened due to confluency. 5. The number of attached cells was highest in low roughness anodized group. And the machined group had significantly lower cell count than any other groups(P<.05). 6. Total protein contents showed no difference among groups. 7. The level of alkaline phosphatase activities was higher in the anodized groups than electropolished and machined groups(P<.05).

• The Journal of Advanced Prosthodontics
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• v.6 no.6
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• pp.498-504
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• 2014

PURPOSE. Dental implant has gained clinical success over last decade with the major drawback related to osseointegration as properties of metal (Titanium) are different from human bone. Currently implant procedures include endosseous type of dental implants with nanoscale surface characteristics. The objective of this review article is to summarize the role of nanotopography on titanium dental implant surfaces in order to improve osseointegration and various techniques that can generate nanoscale topographic features to titanium implants. MATERIALS AND METHODS. A systematic electronic search of English language peer reviewed dental literature was performed for articles published between December 1987 to January 2012. Search was conducted in Medline, PubMed and Google scholar supplemented by hand searching of selected journals. 101 articles were assigned to full text analysis. Articles were selected according to inclusion and exclusion criterion. All articles were screened according to inclusion standard. 39 articles were included in the analysis. RESULTS. Out of 39 studies, seven studies demonstrated that bone implant contact increases with increase in surface roughness. Five studies showed comparative evaluation of techniques producing microtopography and nanotopography. Eight studies concluded that osteoblasts preferably adhere to nano structure as compared to smooth surface. Six studies illustrated that nanotopography modify implant surface and their properties. Thirteen studies described techniques to produce nano roughness. CONCLUSION. Modification of dental osseous implants at nanoscale level produced by various techniques can alter biological responses that may improve osseointegration and dental implant procedures.