• 한국초지조사료학회지
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• 제38권2호
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• pp.140-144
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• 2018

To investigate the impact of nitrogen (N) mineral on reproductive potential of Brassica napus L, plants were treated with different levels of N treatment ($N_0$; $N_{100}$; $N_{500}$). The half of N content for each treatment were applied at the beginning of the early vegetative stage and the rest was applied at the late vegetative stage. Nitrogen content in plant tissues such as root, stem and branch, leaf, pod and seed was analyzed and harvest index (HI) was calculated as percentage of seed yield to total plant weight. Biomass and nitrogen content were significantly affected by different levels of N supply. Biomass was significantly decreased by 59.2% in nitrogen deficiency ($N_0$) but significantly increased by 50.3% in N excess ($N_{500}$), compared to control ($N_{100}$). Nitrogen content in all organs was remarkably increased with nitrogen levels. N distribution to stem and branches, and dead leaves was higher in N-deficient ($N_0$) and N excessive plants ($N_{500}$) than in control ($N_{100}$). However, nitrogen allocated to seed was higher in control ($N_{100}$) than in other treatments ($N_0$ or $N_{500}$), accompanied by higher HI. These results indicate that the optimum level of N supply ($N_{100}$) improve HI and N distribution to seed and excessive N input is unnecessary.

• 한국응용곤충학회지
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• 제14권1호
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• pp.29-33
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• 1975

저항성유기인계농약인 fenitrothion 유제를 8개품종이 수도체에 살포시용하고, 부착 및 침투된 주성분의 경시적변화와 곡위중의 집적잔류량을 GLC로 분석, 검토한 바 그 결과를 요약하면 다음과 같다. 1) FTD/GLC에 있어서 $10\%$ Silicone DC-200 column으로 nano-gram 이하까지 fenitrothion 주성분의 검출이 가능한 조작조건을 밝혔다. 2) 출수전의 수도체에 $0.1\%$ fenitrothion을 반당 20l의 농산로 살포한 바, 전엽에 평균 15.32ppm이 부착, 24시간후에 1.67ppm(약 $11\%$)이 잔존하였고, 살포 24시간후의 침투량은 6.75ppm($44\%$)으로, 24 시간경과후 2.61ppm($39\%$이 잔존하고 그후의 감소는 완만하며, 침투분의 잔존율이 높았다. 3) 엽신과 엽초별 침투량은 엽신이 월등히 높았으나 잔존율은 비등하였고, 침투 3일이내에 급격한 감소(약$90\%$)를 보이고, 그 후는 완만한 감소로 10일후에는 약 $3\%$의 잔존율을 유지하였다. 4) 곡위중의 fenitrothion 잔류량은 왕겨에 0.02-0.05ppm(평균 0.04ppm), 현미에 0.01 ppm-0.02ppm(평균 trace)수준으로 왕겨중함량의 약 $22\%$ 해당하며, WHO/FAO의 권고잔류허용량 (과채류는 0.1-0.5 ppm, 곡류는 미설정)에 훨씬 미달되는 수치였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제18권3호
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• pp.411-427
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• 1996

The purpose of this study was to observe the healing process and the distribution of fibronectin in injured condylar cartilage and bone by using LM and SEM. In order to perform this study, 40 male rat, weighing about 250g were selected. Under general anesthesia with Pentobarbital sodium, condylar cartilage and neck bone were resected. Then, the wound was irrigated with saline and closed with 5-0 chromic catgut and 4-0 silk by layer-to-layer suturing. The experimental rats were sacrificed by perfusion with 3% paraformaldehyde at 1st and 4th week after operation. The condylar process and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. The histological observation of the specimens in LM level was performed after H-E stain and Azan stain. For localization of fibronectin, immunostaining was achieved by the avidin-biotin complex method. To study the change on condylar surface, the specimens were dehydrated, dried, gold coated and were observed with a scanning electron microscope(Hitachi S-2300). The results were as follows ; 1. The cartilage group and the bone group were repaired with epiphyseal cartilage layer on the cut surface as the normal control group. 2. The cut surface was repaired more quickly in the cartilage group than in the bone group. 3. Chondrocytes, diferentiated during healing, were stained strongly to anti-fibronectin, and fibronectin was supposed to participatein chondrocyte differentiation and cartilagenous matrix formation. 4. Fibronectin was distributed more in the new bone than in the old bone, and the osteoblasts surrounding it were also stained strongly. Fibronectin was supposed to participate in new bone matrix formation. 5. Fibronectin is supposed to be associated with the differentiation, migration and adhesion of chondrocyte and osteoblast and to participate in endochondral bone formation.

• 대한치과보철학회지
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• 제33권3호
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• pp.413-439
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• 1995

The purpose of this study was to evaluate the stress distribution developed in the supporting structures by mandibular distal extension removable partial dentures with 2 different direct retainer designs and with or without indirect retainer and abutment splinting. The examined direct retainers on the second bicuspid abutment tooth were Akers clasp and RPA clasp, the indirect retainer was located on the mesial fossa of the first bicuspid, and the first and second bicuspid were splinted in case of tooth splinting. Total 8 cases were compared and analyzed with 3-dimensional finite element method. 150N were applied vertically on the artificial teeth of the removable partial denture, and then stress distribution patterns were analyzed and compared. The results were as follows : 1. The forces transmitted to the abutment tooth were primarily from the occlusal rests. 2. The abutment tooth was displaced distally when the force was applied. The compressive stress was observed at the distal root surface of the abutment tooth and the tensile stress, at the mesial root surface. 3. The denture base was displaced posteriorly and inferiorly when the force was applied. At the more distal portion of the denture base, the greater displacement was observed.And the anterior portion of the major connector was displaced superiorly. 4. The occlusal rest placed on the distal part of the abutment tooth tended to tip the tooth more posteriorly than did one on the mesial part of that tooth. 5. Severe superior displacement was observed at the anterior portion of the major connector in case of removable partial dentures without indirect retainer. 6. In case of tooth-splinting, the stress was distributed through all the root surface of both abuments. In case of no tooth-splinting, the stress was concentrated on the distal root surface of the primary abutment.

• Ocean and Polar Research
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• 제33권1호
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• pp.45-53
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• 2011

Distribution of Polychlorinated biphenyls (PCBs) and organochlorine pesticides in sediments, seawater and fish tissues were examined. Concentrations of ${\Sigma}PCBs$ and ${\Sigma}DDTs$ in the intertidal sediments of Asan Bay ranged from $1.90{\sim}3.27\;ng\;g^{-1}$ dw (dry weight) and $0.51{\sim}1.26\;ng\;g^{-1}$ dw, respectively. ${\Sigma}PCBs$ concentrations in the tissue of greenling (Hexagrammos otakii), olive flounder (Paralichthys olivaceus) and finespotted flounder (Pleuronichthys cornutus) were $1.27{\pm}0.55$, $1.30{\pm}0.71$ and $1.19{\pm}0.43\;ng\;g^{-1}$ ww (wet weight), respectively. The profile of PCBs in the sediments and the 3 species of fish differed as witnessed by the dominance of tetra- to penta-chlorinated congeners in sediments while penta- to hexa-chlorinated congeners dominated in fish. ${\Sigma}DDTs$ concentrations were $0.67{\pm}0.52$, $0.79{\pm}0.61$, $1.58{\pm}1.05\;ng\;g^{-1}$ ww respectively in the tissue of greenling, olive flounder and finespotted flounder. The p,p'-DDE appeared to be the most important metabolite of DDT in studied fishes. The concentrations of PCBs and organochlorine pesticides measured in this study for fish were generally lower than those of previous studies. Compared to the pollutant concentration quality guidelines in marine fish, the environment of Asan Bay appears to be in a healthy state.

• Toxicological Research
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• 제17권2호
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• pp.73-82
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• 2001

The incidence and distribution of necrotic and apoptotic neural cells, and activated astrocytes in the brain of rats intoxicated intra peritoneally with diisopropylfluorophosphate were investigated. Pyridostigmine bromide (0.1 mg/kg) and atropine methylnitrate (20 mg/kg) were pretreated intramuscularly 30 min and 10 min, respectively, prior to diisopropylfluorophosphate (4-10 mg/kg) administration. Diisopropylfluorophosphate induced severe limbic seizures, early necrotic and delayed apoptotic brain injuries, and rapid astrocytic responses. The necrosis, which was closely related to seizure intensity, was observed as early as 1 hr after intoxication predominently in hippocampal pyramidal cells, cerebellar Purkinje cells and neurons in pyriform/entorhinal cortices, showing malacia of neurophils. In contrast, apoptosis started to appear 12 hr after intoxication in neurons in thalamus, amygdala and neocortex, and ephendymal cells surrounding the 4th ventricle. Since marked apoptosis was induced in rats exhibiting relatively-low seizure intensity, the degree of necrosis and apoptosis was shifted to each type of injury according to the seizure intensity. Activated astrocytes, observed within 1 hr along the limbic system, were suggested to affect the neural injury patterns by producing high level of nitric oxide. However, the distribution of activated astrocytes was not in parallel with those of necrotic or apoptotic injuries, implying that the astrocytic responses resulted from seizure activity rather than neural injuries. Furthermore, astrocytes in malacic tissues disappeared during the severe limbic seizures. Therefore, it would be one of the cautionary notes on the expression of glial fibrillary acidic protein in astrocytes as a biochemical marker of brain injuries following acute exposure to organophosphates.

• Asian Pacific Journal of Cancer Prevention
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• 제17권2호
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• pp.835-838
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• 2016

Background: Lung cancer remains one of the most common cancers in the world, both in terms of new cases (about 13% of total per year) and deaths (nearly one cancer death in five), because of the high case fatality. Errors in lung cancer type or malignant growth determination lead to degraded treatment efficacy, because anticancer strategy depends on tumor morphology. Materials and Methods: We have made an attempt to evaluate effectiveness of machine learning algorithms in the task of lung cancer classification based on gene expression levels. We processed four publicly available data sets. The Dana-Farber Cancer Institute data set contains 203 samples and the task was to classify four cancer types and sound tissue samples. With the University of Michigan data set of 96 samples, the task was to execute a binary classification of adenocarcinoma and non-neoplastic tissues. The University of Toronto data set contains 39 samples and the task was to detect recurrence, while with the Brigham and Women's Hospital data set of 181 samples it was to make a binary classification of malignant pleural mesothelioma and adenocarcinoma. We used the k-nearest neighbor algorithm (k=1, k=5, k=10), naive Bayes classifier with assumption of both a normal distribution of attributes and a distribution through histograms, support vector machine and C4.5 decision tree. Effectiveness of machine learning algorithms was evaluated with the Matthews correlation coefficient. Results: The support vector machine method showed best results among data sets from the Dana-Farber Cancer Institute and Brigham and Women's Hospital. All algorithms with the exception of the C4.5 decision tree showed maximum potential effectiveness in the University of Michigan data set. However, the C4.5 decision tree showed best results for the University of Toronto data set. Conclusions: Machine learning algorithms can be used for lung cancer morphology classification and similar tasks based on gene expression level evaluation.

• 한국안광학회지
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• 제19권3호
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• pp.413-418
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• 2014

목적: 한국관박쥐 망막의 기능을 알아보기 위해서 흥분성 신경전달물질인 글루타메이트 수용체의 분포도를 분석하였다. 방법: 성체 한국관 박쥐의 망막을 $40{\mu}m$ 수직 절편 한 후 표준면역세포화학법을 이용하였다. 면역형광이미지는 Bio-Rad MRC 1024 공초점 현미경을 사용하여 얻었다. 결과: AMPA (GluR1-4), Kainate (GluR5-7, KA1-2), NMDA (1, 2A, 2B)는 내망상층과 외망상층에 주로 분포되어 있었다. KA1은 신경절세포층에도 많은 수의 수용체가 존재하였다. 결론: 한국관박쥐는 포유류망막에 있는 신경세포와 신경전달물질을 동일하게 가지고 있었다. 한국관박쥐도 기능적 망막을 가지고 있음을 제시한다.

• 한국토양비료학회지
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• 제49권6호
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• pp.644-650
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• 2016

In soil-to-plant transfer of heavy metals, the amount absorbed and accumulated varies depending on the environment conditions. The absorption rate of cadmium (Cd) in plants differs considerably depending on the bioavailability of Cd in the soil, while usage by various organic matters is also reported to affect absorption patterns. Therefore, this study aimed to identify the difference in the transfer of essential metal ions and Cd to various plant parts when rice straw compost was used to cultivate soybean (Glycine max L. cv. Daepung). In the two-leaf stage of soybean cultivated in a greenhouse, Cd was mixed in the soil, after which the Cd and essential metal ions contents, and physiological changes of soybean seedlings were studied on the 15th and 25th day. The Cd toxicity in the plant was reduced with the use of rice straw compost. Further, the Cd content varied with the plant part, and was higher in young leaves (3rd and 4th leaf) than in the stem. When analyzed by leaf age, the Cd transfer was highest in young leaves (3rd and 4th leaf), followed by mature leaves (1st and 2nd leaf). While there was no significant difference between plant tissues in the absorption rate of copper (Cu) and zinc (Zn) when rice straw compost was used against Cd toxicity, the absorption rate of manganese (Mn) and iron (Fe) showed a significant decline in both the control and rice straw compost treatment conditions, as well as a significant difference between leaf ages. Therefore, these results confirm that the use of rice straw compost against Cd toxicity is effective, and implies that the rate of Cd transfer in the soybean plant varies significantly with leaf age.

• 대한치과보철학회지
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• 제44권5호
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• pp.584-593
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• 2006

Statement of problem : Dental implant which has been developed gradually through many experiments and clinical applications is presently used to various dental prosthetic treatments. To conduct a successful function of implant prosthesis in oral cavity for a long time, it is important that not only structure materials must have the biocompatibility, but also the prosthesis must be designed for the stress, which is occurred in occlusion, to scatter adequately of load support. Therefore, it is essential to give the consideration about the stress analysis of supporting tissues for higher successful rates. Purpose : Recently, many implant manufacturing company produce various taper design of root form implant, the fixture is often selected. However, the stress analysis of taper form fixture still requires more studies. Material and method : This study we make the element models that five implant fixture; Branemark system(Nobel Biocare, Gothenberg, Sweden), Camlog system(Altatec, Germany), Astra system(Astra Tech, Sweden), 3i system(Implant Innovations Inc, USA), Avana system(Osstem, Korea) were placed in the area of mandibular first premolar and prosthesis fabricated, which we compared with stress distribution using the three-dimension finite element analysis under two loading condition. Results : This study compares the aspect of stress distribution of each system with the standard of Von mises stress, among many resulted from finite element analysis so that this research gets the following results. 1. In all implant system, oblique loading of maximum Von mises stress of implant, alveolar bone and crown is higher than vertical loading of those. 2. Regardless of loading conditions and type of system, cortical bone which contacts with implant fixture top area has high stress, and cancellous bone has a little stress. under the vertical loading, maximum Von mises stress is more higher in order of Branemark, Camlog, Astra, 3i, Avana. under the horizontal loading, maximum Von mises is more higher in order of Camlog, Branemark, Astra, 3i, Avana.