• Journal of Plant Biotechnology
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• 제37권4호
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• pp.379-387
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• 2010

Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.414-424
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• 2010

This report describes recent advances in tissue culture, genetic transformation of commercial rose (Rosa hybrida) and in development of new rose cultivars by molecular breeding. Rose is one of major cut-flowers in global horticulture industry. Successful progresses were made in development of new cultivars for pathogen resistant, environmental stress resistant and petal color modification by molecular breeding. New cultivars, however, has not reported yet in korea, although lots of progresses were achieved in each field of conventional breeding, tissue culture and genetic transformation. Cooperation in these research fields will promote screening of useful genes to have specific traits on rose and exploiting of processes to improve in the efficiency of tissue culture and genetic transformation of rose, therefore, we hopefully expect that new rose cultivars by molecular breeding will be released in the near future.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.456-464
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• 2010

Potato is one of the most important crops in the world. Due to vegetative propagation of this crop, techniques of plant tissue culture and genetic transformation are often applied for potato researches and a lot of progress has been made in the breeding programs using these techniques during the last decades. In potato, there have been several trials to introduce GM potato varieties to the world market, but they so far failed due to the changed legislation and unwillingness of large processors to process GM potatoes. These issues are highly associated with the general acceptances of the public and other political decisions. In addition to these, there are still obstacles to overcome to achieve the development of commercial potato variety and several factors to improve horticulturally important traits. In this study, therefore, we reviewed recent advances and research status on tissue culture and genetic transformation in potato and discussed future perspective.

• Plant Breeding and Biotechnology
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• 제6권4호
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• pp.426-433
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• 2018

Plant tissue culture is a technique that has invariably been used for various purposes such as obtaining transgenic plants for crop improvement or functional analysis of genes. However, this process can be associated with a variety of genetic and epigenetic instabilities in regenerated plants, termed as somaclonal variation. In this study, we investigated mutation spectrum, chromosomal distributions of nucleotide substitution types of single-nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) by whole genome re-sequencing between Dongjin and Nipponbare along with regenerated plants of Dongjin from different induction periods. Results indicated that molecular spectrum of mutations in regenerated rice against Dongjin genome ranged from $9.14{\times}10^{-5}$ to $1.37{\times}10^{-4}$ during one- to three-month callus inductions, while natural mutation rate between Dongjin and Nipponbare genomes was $6.97{\times}10^{-4}$. Non-random chromosome distribution of SNP and InDel was observed in both regenerants and Dongjin genomes, with the highest densities on chromosome 11. The transition to transversion ratio was 2.25 in common SNPs of regenerants against Dongjin genome with the highest C/T transition frequency, which was similar to that of Dongjin against Nipponbare genome.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.114-118
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• 2019

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

• 한국작물학회지
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• 제37권5호
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• pp.419-424
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• 1992

콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다.

• Journal of Biosystems Engineering
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• 제32권2호
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• pp.109-114
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• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

• Journal of Periodontal and Implant Science
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• 제32권1호
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• pp.129-142
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• 2002

Epithelial-mesenchymal interaction plays a important role in cell growth and differentiation. This interaction is already well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular microenvironment which provide a epithelial-mesenchymal interaction. Because conventional monolayer culture lacks epithelial-mensenchymal interaction, cultivated cells have an morphologic, biochemical, and functional characteristics differ from in vivo tissue. Moreover, it's condition is not able to induce cellular differention due to submerged culture condition. Therefore, the aims of this study were to develop and evaualte the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted oral keratinocytes by histological and immunohistochemical analysis. The results were as follow; 1. Gingival keratinocytes reconstituted by three-dimensional organotypic culture revealed similar morphologic characteristics to biopsied patient specimen showing stratification, hyperkeratinosis, matutation of epithelial architecture. 2. Connective tissue structure was matured, and there is no difference during stratification period of epithelial 3-dimensional culture. 3. The longer of air-exposure culture on three-dimensionally reconstituted cells, the more epithelial maturation, increased epithelial thickness and surface keratinization 4. In reconstitued mucosa, the whole epidermis was positively stained by anti-involucrin antibody, and there is no difference according to air-exposured culture period. 5. The Hsp was expressed in the epithelial layer of three-dimensionally cultured cells, especially basal layer of epidermis. The change of Hsp expression was not significant by culture stratification. 6. Connexin 43, marker of cell-cell communication was revealed mild immunodeposition in reconstitued epithelium, and there is no significant expression change during stratification. These results suggest that three-dimensional oragnotypic co-culture of normal gingival keratinocytes with dermal equivalent consisting type I collagen and gingival fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. And this culture system seems to provide adequate micro-environment for in vitro tissue reconstitution. Therefore, further study will be focused to study of in vitro gingivitis model, development of novel perioodntal disease therapeutics and epithelial-mensenchymal interaction.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.307-312
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• 2004

식물조직배양용 바이오리액터 8개의 배양액 P보, DO 농도를 on-line으로 계측하고 pH 농도 및 주입공기 량을 제어할 수 있는 시스템을 개발하였다. 시스템 제어용 컴퓨터 프로그램은 나리구근의 성장단계에 맞는 주입공기량을 추종제어방식으로 제어하며, 바이오리액터 내부의 pH 변화를 감지하여 오염을 감시하는 오염경보기능이 포함되어있다. 성장단계에 따라 적절한 주입공기량의 선정을 위하여 시뮬레이션 한 결과 배양초기에는 300 cc/min, 20일 경과 후에는 400 cc/mim, 40일 경과 후에는 500 cc/min, 60일 경과 후에는 600 cc/min, 그리고 80일 경과 후부터는 700 cc/min의 공기를 주입할 경우 바이오리액터내 나리구근의 분포가 고르게 나타났으며, 이 결과를 바이오리액터 배양실험에 이용하였다 배양액의 pH 농도 제어 시스템은 배양 전 기간동안에 제어 목표 값 (5.5$\pm$0.5)로 제어할 수 있었다.

• 식물조직배양학회지
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• 제28권3호
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• pp.123-128
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• 2001

인삼 callus의 기내배양에 의해서 항암물질을 대량생산하기 위한 연구의 일환으로 인삼 우수계통으로부터 기내에서 callus를 유기하여 polyacetylene 고함유 세포주를 선발하고, 인삼 callus의 polyacetylene 생산에 미치는 식물호르몬 및 배지의 영향을 조사하였다. 식물호르몬 CPA가 첨가된 MS배지에서 유도된 인삼 우수계통 callus에서는 polyacetylene 중에서 panaxynol은 TLC와 GC에 의해서 전혀 검출할 수 없었으나, callus에 따라서는 panaxydol이 형성됨을 확인할 수 있었다. 특히, 강력한 항암효과를 가지고 있는 panaxydol은 우수계통 callus 18종 중에서 5종이 형성하였으며, 30201계통 callus에서 가장 많이 검출되었다. 또한 인삼 10301계통 callus는 CPA가 단독으로 첨가된 배지에서 panaxydol을 생성하지 못하였으나, CPA 2 mg/L와 BA 0.05 mg/L 첨가구에서는 callus의 생장도 양호하였으며, panaxydol도 생성하였다. 인삼 callus의 생장과 panaxydol의 합성에는 MS배지보다 SH 배지가 더 양호하였으며, NAA와 sucrose는 전혀 영향을 미치지 않았다.