• Title/Summary/Keyword: Tissue pattern

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Iris Pattern Positioning with Preserved Edge Detector and Overlay Matching

  • Ryu, Kwang-Ryol
    • Journal of information and communication convergence engineering
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    • v.8 no.3
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    • pp.339-342
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    • 2010
  • An iris image pattern positioning with preserved edge detector, ring zone and clock zone, frequency distribution and overlay matching is presented in this paper. Edge detector is required to be powerful and detail. That is proposed by overlaying Canny with LOG (CLOG). The two reference patterns are made from allocating each gray level on the clock zone and ring zone respectively. The normalized target image is overlaid with the clock zone reference pattern and the ring zone pattern to extract overlapped number, and make a matched frequency distribution to look through a symptom and position of human organ and tissue. The iterating experiments result in the ring and clock zone positioning evaluation.

Canine nervous-tissue tumors with features of peripheral nerve sheath tumor: histopathological and immunohistochemical findings (말초신경초 종양의 특징을 지닌 개 신경종의 조직병리학적 및 면역조직화학적 진단)

  • Lee, Sun-Gue;Lee, Jae-Ha;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.41 no.1
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    • pp.57-61
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    • 2018
  • Canine peripheral nerve sheath tumors (PNSTs) are spindle cell tumors that arise from Schwann cells, perineural cells, fibroblasts or all of them. Based on the morphology and biologic behavior, PNSTs are divided into benign PNST (BPNST) and malignant PNST (MPNST) forms. The aim of this study is to diagnose the two cases of neoplastic tissue samples with features of PNSTs by the histopathology and immunohistochemistry. The study was performed using two specimens from small animal clinic. The first case, A was a mass, 3~4 cm in diameter, extruded from vaginal mucosa of 10-year-old spayed female mixed-breed dog. And the second case, B was a subcutaneous mass, 1.5 cm in diameter, which is originated from right hind leg of 9-year-old castrated male mixed-breed dog. Two cases were stained with hematoxylin and eosin (H&E) for histopathological examination. And also immunohistochemistry (IHC) was performed by the avidin-biotin peroxidase complex (ABC) method with antibodies specific for the following proteins: S-100 protein, smooth muscle actin (SMA) and epidermal growth factor receptor (EGFR). In results, Antoni B schwannoma pattern characterized by pleomorphic, round and fusiform polygonal cells was seen in A. In B, Antoni A pattern, densely packed spindle cells arranged in interlacing bundles was seen in addition to Antoni B pattern. In IHC, cytoplasms of neoplastic cells were diffusely labeled for S-100 expression in A and B. For SMA, both A and B show negative expression. And for EGFR, A shows negative expression but B shows partially positive expression in areas of Antoni B schwannoma pattern. The histopathologic features of two cases coupled with the S-100 immunoreactivity led to a diagnosis of PNST. For SMA, both A and B show negative expression. The diagnosis of A will be a BPNST with the negative result and B will be a MPNST with the positive result for EGFR.

The Effect of Botulinum Toxin-A on the Survival of Random-Pattern Cutaneous Flap in Rat (보튤리눔 톡신-A가 백서의 임의 피판 생존율에 미치는 영향)

  • Kim, Young Seok;Lee, Chae Su;Yoo, Won Min;Tark, Kwan Chul
    • Archives of Plastic Surgery
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    • v.35 no.4
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    • pp.360-366
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    • 2008
  • Purpose: Botulinum toxin type A(BoTA) can block the release of vasoconstriction cotransmitters as well as acetylcholine in nerve terminal. The authors observed that BoTA increases flap survival by preventing sympathetic collapse of peripheral vessels. Methods: 10 Sprague Dawley rats were divided into control(n=5), and BoTA group(n=5). $3{\times}10cm$ sized random pattern cutaneous flaps were elevated on the dorsal side in both groups. In BoTA group, BoTA was injected into the flap via intradermal to subdermal route, 7 days before the flap elevation. Flap survival rates (survival area/total area) were measured 7 days after the elevation. Cutaneous blood flow was measured in proximal, middle and distal compartments of the flap using laser Doppler flowmetry initially, preoperatively, at immediate postoperation, and 7 days after flap elevation, respectively. Histological examination was performed 7 days after the flap elevation. The number and shape of the vessels were evaluated under microscope. Results: Mean flap survival was $53.18{\pm}6.58%$ in control group and $93.79{\pm}6.06%$ in BoTA group, displaying statistically significant difference(p=0.0008, p<0.05). In the control group, blood flow to the middle and distal compartments of the flap decreased significantly immediately after flap elevation. In the BoTA group, blood flow to the middle compartment did not decrease(p=0.002) and slightly decreased in the distal compartment(p=0.001). Cutaneous blood flow was significantly higher in all compartments of the flap in BoTA group than in control group, 7 days after the flap elevation. In histopathologic examination, greater number of vessels were noted in the BoTA group than in the control group. Conclusion: Botulinum toxin A can increase the survival of the random pattern cutaneous flap in rats by preventing the sympathetic collapse of peripheral vessels.

Variation of Peroxidase 8 in Maize, Zea mays, L. (옥수수에 있어서 Peroxidase 8의 변이)

  • Bong Ho, Choe
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.3
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    • pp.374-378
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    • 1983
  • As one way of evaluating polymorphism in maize, variation of peroxidase 8 (Px.8) in maize plants was investigated by means of horizontal acrylomide electrophoresis. The specific part of maize plants was stele and other parts of plants were also studied for 34 different maize lines and hybrids. The results obtained indicate that Px.8 has three distinct migrating patterns on gel such as fast, slow and fast-slow. The band pattern varied with materials used. Most hybrids were showing fast-slow band, indicating that those hybrids are heterozygous at least for Px.8 allele. Variation of band pattern was obseerved within a single inbred line. The Px.8 in stele tissue and in young leaf and nodal tissue was found to be identical, indicating the possible use of those tissues as an alternative tissue for Px.8 study. It was also found that there might be some definite relationship between Px.8 and Px.3 activities in the same tissue.

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Clinical Application and Limitations of the Capsular Pattern (관절낭 패턴의 임상적 적용과 한계)

  • Lim, Wootaek
    • Physical Therapy Korea
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    • v.28 no.1
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    • pp.13-17
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    • 2021
  • A normal range of motion is essential for performing activities of daily living. The capsular pattern is the proportional motion restriction in range of motion during passive exercises due to tightness of the joint capsule. Although the capsular pattern is widely referred to in clinical practice, there is no scientific evidence to support the concept. In this review, the appropriateness of the capsular pattern for evaluation of joint pathology was assessed. In the Textbook of Orthopaedic Medicine written by Cyriax, the capsular pattern did not specify how much reduction in angular motion is considered motion restriction. As the definition proposed initially was unclear, different methods have been used in previous studies investigating capsular pattern. In addition, the capsular pattern described all the major joints of the human body, but only the hip joint, knee joint, and shoulder joint were studied in experimental studies. Sensitivity and specificity were reported in one study and were meaningful in specific pathologies (loss of extension to loss of flexion). There was no consensus on the reliability and validity. In summary, the capsular pattern suggested by Cyriax or Kaltenborn is not supported or applies only to certain conditions. Various components around a joint complement each other and provide stability to the joint. It is recommended that the therapist perform multiple assessments rather than rely on a single assessment when evaluating joints.

Study in the Respiratory Metabolism in Some Bivalves(II) on the Oxidative Metabolism and its Enzyme System in the Gill Tissue of the Fresh Water Mussel, Cristaria plicata spatiosa (CLESSIN) (패류의 호흡대사에 관한 연구(II) 담수산 패류, Cristaria plicata spatiosa (CLESSIN), 아가미 조직의 산화적 대사와 그 효소분에 대하여)

  • 한문희;김동준;최희정
    • The Korean Journal of Zoology
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    • v.4 no.1
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    • pp.7-12
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    • 1961
  • 1) Respiratory metabolism patterns and its enzyme systems in the gill tissue of the fresh water mussels, Cristaria plicata were investigated through the examination on the effects of respiratory enzyme inhibitors, (KCN, NAF) and succinoxidase assay, while studying the effects of neutral salts (NaCL, KCL, CaCl2) and pH on oxygen consumption of the gill tissue. 2) In the limited concentration of KCL (0.3mM) and NaCl (0.4mM) solutions, oxygen consumption of the intact gill tissue was accelerated, but in CaCl2(0.5mM) solution, it showed no significant effect. The oxygen consumption was gradually decreased at the above concentrations of these limitations. The optimum pH for the respiration of the gill was 7.3. 3)Cyanide in 10-8M solution inhibited 88.8% of the respiration of the intact gill tissue. Methylene blue accelerated the respiration of the noral gill tissue, and slightly but significantly reversed the cyaniide poisoned respiration. 4)Oxygen consumption of the gill homogenate was apparently increased by the mixed addition of succinate, cytochrome c and activators (AlCl3 and CaCl2). This results suggested that succinoxidase system acts on the respiratory pattern of the gil tissue. 5) It was able to recognize that the enolase, which acts on the anaerobic glycolytic system, participated in the tissue respiration of the gill for NaF in 5$\times$10-2 M solution inhibited 55.5% of the respiration of the same intact tissue.

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Effect of Skin Tissue Necrosis Relaxation by Low Frequency Pulsed Electromagnetic Fields (LF-PEMF) Stimulation (저주파 펄스 전자기장 자극에 의한 피부 조직괴사 완화 효과)

  • Lee, Jawoo;Kim, Junyoung;Lee, Yongheum
    • Journal of Biomedical Engineering Research
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    • v.42 no.1
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    • pp.25-30
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    • 2021
  • Objective: The aim of this study is to consider the effect of skin tissue necrosis by improving blood flow in animal skin models for low frequency pulsed electromagnetic fields (LF_PEMF) stimulation. Methods: Twenty rats (Wistar EPM-1 male, 280-320 g) were randomly divided into control groups (n=10) and the PEMF groups (n=10). To induce necrosis of the skin tissue, skin flap was treated in the back of the rat, followed by isolation film and skin flap suturing. Subsequently, the degree of necrosis of the skin tissue was observed for 7 days. The control group did not perform any stimulation after the procedure. For the PEMF group, LF_PEMF (1 Hz, 10 mT) was stimulated in the skin flap area, for 30 minutes a day and 7 days. Cross-polarization images were acquired at the site and skin tissue necrosis patterns were analyzed. Results: In the control group, skin tissue necrosis progressed rapidly over time. In the PEMF group, skin tissue necrosis was slower than the control group. In particular, no further skin tissue necrosis progress on the day 6. Over time, a statistically significant difference from the continuous necrosis progression pattern in the control group was identified (p<0.05). Conclusions: It was confirmed that low frequency pulsed electromagnetic fields (LF_PEMF) stimulation can induce relaxation of skin tissue necrosis.

Tissue Specific Expression of Tomato Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Plants (형질전환 담배에서 토마토 PAL유전자의 조직 특이적 발현)

  • YI, Jung-Yoon;Lee, Shin-Woo;SEO, Hyo-Won;PARK, Kuen-Woo
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.2
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    • pp.89-93
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    • 1998
  • Tomato phenylalanine ammonia-lyase 5 (tPAL5) was identified that alternate initiation sites were utilized differentially in response to environmental stimuli (Lee et al, 1992b). In this study, we tried to look into tissue -or cell- specific expression pattern of tPAL5 gene by fusing with ${\beta}-glucuronidase$ (GUS) gene in transgenic tobacco plants. In transgenic plants, root and stem extracts contained 8~12 fold higher levels of GUS activity than petiole or leaf tissue while the highest levels of induction was observed from leaf tissue by mechanical wounding (5~11 fold). In trans-sections of stems and petioles, GUS activity was restricted to phloem cells(outer region) of developing vascular bundle and mainly at apical tip region in the root tissues. The levels of GUS activity was drastically reduced (10~12 fold reduction) when the 5'-upstream region of tPAL5 gene (-1151bp from ATG codon) was deleted up to -665. The levels of GUS expression, however, raised up by 6~8 fold when deleted up to -455. Therefore, we conclude that there are positive cis-elements at the region -1151 to -1008 and at -455 to -195 while the negative cis-element is at -1008 to -455.

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A Study on the Occurrence Pattern of Vacant Spaces as the Decline Index in Old Hillside Residential Area (노후 경사주거지 쇠퇴지표로서의 유휴공간 발생패턴 연구)

  • Jung, In-A;Woo, Shin-Koo
    • Journal of the Architectural Institute of Korea Planning & Design
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    • v.34 no.3
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    • pp.93-104
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    • 2018
  • This study is about occurrence pattern of vacant spaces as a urban decline indicator to consider a decline of old hillside residential area from spatial structure perspective. For investigation based upon vacant space and the street structure, this study selects Ami Chojang-dong in Seo-gu, Busan, of which 79.7% of total area is over a gradient of $10^{\circ}$. Focused on relationship between occurrence pattern of vacant spaces and 'vertical alleys(link)' serving as main movement path of local residents in hillside residential area, the length, numbers, slopes, and use characteristic of vertical alleys were analyzed using Arc GIS 9.3 program to classify characteristic zone types of spacial structure in hillside residential area. After the occurrence pattern of vacant space are interpreted in relation with building density, vertical alleys' number length slope, the social and economic issues have been considered as well. After analyzing the occurrence pattern of vacant space, this study argues that the occurrence pattern of vacant space in old hillside residential area is rather affected by street structure system consisting of vertical alleys than the impacts of social economical issues.

Tissue Distribution of $TiO_2$ Nanoparticles in Mice after Oral Administration, and Skin Treatment (마우스 경구 및 경피투여에 의한 $TiO_2$ 나노입자의 체내분포)

  • Park, Eun-Jung;Park, Kwang-Sik
    • Environmental Analysis Health and Toxicology
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    • v.23 no.1
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    • pp.63-65
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    • 2008
  • The tissue distribution of $TiO_2$, nanopaprticles was investigated in mice after oral administration, and skin treatment. Male mice were treated with the dose of 5 g/kg of $TiO_2$ for three consecutive days and sacrificed at 24 hours after the last administration. As results, the orally administered $TiO_2$ nanoparticels were shown to be distributed in the testis, lung, and brain at 24 hours after the last treatment. Kidney does not seem to be the main target of $TiO_2$ nanoparticle distribution. It means that $TiO_2$ nanoparticles (17 nm) are easily absorbed through entero-gastric system and may cause toxicity in brain, lung, and reproductive organs. The distribution of skin treatment showed the same pattern like oral administration.