• Title/Summary/Keyword: Tissue pattern

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MAGNETIC RESONANCE IMAGING AND HISTOPATHOLOGIC CORRELATIONS OF FOCAL LESIONS INDUCED BY LASER (레이저 조사후 자기공명영상과 조직학적 소견의 상호일치도)

  • 이정구;정필상;정필섭;조정석;김상준
    • Korean Journal of Bronchoesophagology
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    • v.2 no.2
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    • pp.194-199
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    • 1996
  • Laser therapy is becoming an accepted procedure for tissue coagulation and ablation and is especially useful in treating tumors. The laser energy is applied to the tissue of interest through various delivery systems which are introduced percutaneously, via blood vessels, through body openings, or during surgical exposure of the tissue. One of the major obstacles to effective application of lasers has been the lack of reliable method to determine the extent of tissue involvement in real time. Several methods have been proposed for monitoring the tissue response and controlling the laser in real time during laser therapy. Among them, magnetic resonance imaging(MRI) has been introduced to monitor laser-tissue interactions because laser irradiation induces changes not only in the thermal motions of the hydrogen protons within the tissue but also in the distribution and mobility of water and lipids. The buttocks of New Zealand rabbits were treated by KTP and $CO_2$laser(power : 10 watts, exposure time:10 seconds). m images were taken at immediately after lasering, 1 week later, 2 weeks later, and at the same time, tissues were harvested for histopathologic study. We analyzed MR images and histopathologic findigs of laser-treated tissues. The MR images taken immediately after laser treatment showed 3 layer pattern and which was correlated with histopathologic changes. We suggest MRI may become a useful monitoring tools for laser-tissue interaction.

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A Study on the Characteristics of Muscle Relaxation according to the Temperature Condition at the Surface of Imitational Biological Tissue (모의 생체조직의 표면온도 조건에 따른 근육이완 특성에 관한 연구)

  • Ko, Dong-Guk;Lee, Chan-Woo;Kim, Min-Soo
    • Journal of the Korean Society of Mechanical Technology
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    • v.20 no.6
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    • pp.770-775
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    • 2018
  • In this study, the characteristics of muscle relaxation were analyzed by the experimental and numerical method. A skin tissue was produced by imitational biological tissue using the agar powder, saline solution and sugar. The tissue was exposed to three types of wavelength-blue visible radiation(410 nm), red visible radiation(635 nm), and infrared ray(830 nm). The temperature results along the depth of tissue were measured according to the variation of light wavelength and irradiation time. The temperature change of the tissue shown up similar pattern regardless of the light wavelength kinds. The wavelength of infrared ray penetrated strongly into tissue between 3.2 mm and 11.4 mm. Also, the temperature change with the irradiation time was small, and the temperature value of the infrared ray was the largest. As a result, the muscle relaxation will occur mainly at the infrared wavelength.

Cellulose Structures of Primary and Secondary Tissues in Pinus densiflora S. et Z. (소나무재의 1차조직과 2차조직 세포벽 중의 셀룰로오스 구조)

  • Kim, Nam-Hun;Lee, Kee-Young
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.1
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    • pp.60-67
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    • 2001
  • The microscopic characteristics and cellulose structures of primary and secondary tissues in Pinus densiflora S. et Z. were examined. Cells of primary tissue in cross section showed an irregular arrangement and round shape. Fiber lengths were 200 to $250{\mu}m$ in primary tissue, and 1,500 to $1,600{\mu}m$ in secondary tissue. Cell diameters in primary tissue were larger than those in secondary tissue; 40 to $50{\mu}m$ in former and 10 to $20{\mu}m$ in latter. Crystallite width and d-spacing of (200) in both tissues did not show any significant differences. However, crystallinity indices by Segal's method showed significant differences as 23% in primary tissue and 35% in secondary tissue. In the orientation of cellulose microfibril, primary tissues had a random pattern, whereas, secondary tissues presented an oriented pattern with 20 to 30 degree. The cellulose crystalline of primary tissue was easily transformed into cellulose II by mercerization, but that of secondary tissue hardly transformed. It is considered that the difference of crystal transformation in both tissues could be caused by the difference of lignification.

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PERIPHERAL, SOFT TISSUE ODONTOMA : CASE REPORT (Peripheral, soft tissue odontoma에 관한 증례보고)

  • Lee, Kwang-Chul;Choi, Hyung-Jun;Choi, Byung-Jae;Lee, Jong-Gap
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.1
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    • pp.157-161
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    • 1999
  • Odontomas, hamartomas of odontogenic origin, are composed of all the structures that make up teeth. The WHO distinguishes odontoma into two types. The complex odontoma is defined as "a malformation in which all the dental tissues are represented, individual tissues being mainly well-formed but occurring in a more or less disorderly pattern." The compound odontoma is defined as "a malformation in which all the dental tissues are represented in a more orderly pattern than in the complex odontoma, so that the lesion consists of many toothlike structures. Most of these structures do not resemble morphologically the teeth of normal dentition, but in each one enamel, dentine, cementum, and pulp are arranged as in the normal tooth." Almost all odontomas are located intraosseously, but they have occasionally been reported in extrabony location. Peripheral or soft tissue odontomas, those arising outside of the alveolar bone, are very rare. Peripheral or soft tissue odontoma are defined as tumors that demonstrate the histologic characteristics of their intraosseous counterparts but occur solely in the soft tissue covering the tooth-bearing portion of the mandible and maxilla. When they mature, they appear as a radiopaque mass without the peripheral halo. The final diagnosis should be confirmed by biopsy. The origin of peripheral odontoma is probably related to remnants of the dental lamina in the gingiva. The treatment of choice is complete surgical excision, similarly to intraosseous odontoma and it does not tend to recur. This report presents a case of 5-year-old boy with swelling on labial gingiva of primary central incisor. And it was diagnosed as peripheral odontoma by excisional biopsy.

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Development of Tissue mimicking ultrasound phantom materials (Tissue mimicking 초음파 팬텀물질의 개발에 관한 연구)

  • Ma, Sang-Chull;Kong, Young-Kun;Park, Ki-Jung;Lee, Suk
    • Journal of radiological science and technology
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    • v.26 no.1
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    • pp.51-62
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    • 2003
  • We carried out studies on develop of the ultrasound tissue mimicking materials(TMM) by synthesis of polymer urethane(C, CCR, $TiO_2$, tungsten, graphite, silver type). The major finding were as follows; (1) C type TMM was shown good homogeneity, penetration, gray scale like as liver tissue and propagated speed 1,540 m/s, attenuation $0.5{\sim}0.7\;dB/cm/MHz$. (2) $TiO_2$ type TMM was shown heterogeneous dot echo pattern. (3) Silver type TMM was appear good homogeneous echo pattern like as echo texture of thyroid gland. Therefor, C type TMM will be useful for ultrasound Q/A phantom materials and previous phantom materials.

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Expression Pattern of S RNase Gene Promoter in Various Floral Tissues of Lycopersicon peruvianum (일시적 발현을 통한 토마토 S RNase gene promoter의 발현 양상)

  • CHUNG, Il Sun;SHIN Dong Ill;CHUNG, Il Kyung
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.4
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    • pp.237-243
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    • 1998
  • To understand the tissue specific expression pattern of S RNase genes associated with self-incompatibility in L. peruvianum, two promoter regions of $S_{11}$ and $S_{12}$ RNase genes were compared. Homologous sequences between two S RNase gene promoters were found within 300 bp upstream of transcription start site. Moreover short direct repeat sequences within $S_{11}$ RNase gene promoter existed in the vicinity of 350-500 bp upstream of transcription start site. To identify whether the unique promoter sequences of $S_{11}$ RNase gene confer the tissue specific expression, six deletion fragments for $S_{11}$ genomic gene promoter constructed by PCR were fused to $\beta$-glucuronidase gene, and introduced into various tissues of L. peruvianum by microprojectile bombardment. Transient expression assays indicated that $S_{11}$ RNase gene promoter contained the positive and negative regulatory sequences, which can control the floral tissue-specific expression in L. peruvianum.

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Fus Expression Patterns in Developing Tooth

  • Kim, Eun-Jung;Lee, Jong-Min;Jung, Han-Sung
    • Development and Reproduction
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    • v.17 no.3
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    • pp.215-220
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    • 2013
  • Recently, the RNA/DNA-binding protein FUS, Fused in sarcoma, was shown to play a role in growth, differentiation, and morphogenesis in vertebrates. Because little is known about Fus, we investigated its expression pattern in murine tooth development. In situ hybridization of mouse mandibles at specific developmental stages was performed with a DIG-labeled RNA probe. During early tooth development, Fus was detected in the dental epithelium and dental mesenchyme at 11 days postcoitum (dpc) and 12 dpc. From 14 dpc, Fus was strongly expressed in the dental papilla and the cervical loop of the dental epithelium. At postnatal day 4 (PN4), Fus expression was observed in the odontoblasts, ameloblasts, the proliferation zone of the pulp, and the cervical loop. At PN14, the expression pattern of Fus was found to be maintained in the odontoblasts and the proliferation zone of the pulp. Furthermore, Fus expression was especially strong in the Hertwig's epithelial root sheath (HERS). Therefore, this study suggests that Fus may play a role in the HERS during root development.

Extraction of Intercellular Material from Rice Leaf Tissue (수도의 세포간극 물질 추출)

  • Park, W.M.;Son, E.R.;Ko, Y.H.;Yoo, Y.J.;Lee, Y.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.3
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    • pp.323-327
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    • 1983
  • The intercellular material was extracted from rice leaf tissue. The quantitative tests of protein and soluble carbohydrate, and activity of peroxidase, showed differences between tissue extract and intercellular material. Also electrophoretic patterns of peroxidase and esterase isozymes were not similar between them. It indicated that the intercellular material which was extracted, was not the mixture of cellular materials.

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Cell Image Processing Methods for Automatic Cell Pattern Recognition and Morphological Analysis of Mesenchymal Stem Cells - An Algorithm for Cell Classification and Adaptive Brightness Correction -

  • Lim, Kitaek;Park, Soo Hyun;Kim, Jangho;SeonWoo, Hoon;Choung, Pill-Hoon;Chung, Jong Hoon
    • Journal of Biosystems Engineering
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    • v.38 no.1
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    • pp.55-63
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    • 2013
  • Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.

Study on the Analysis of PCBs in Papers by the Peak Pattern Method (피크패턴법을 이용한 종이재의 PCBs 분석 방법에 관한 연구)

  • 김기명;유승석;이광호
    • Journal of Food Hygiene and Safety
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    • v.14 no.1
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    • pp.67-75
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    • 1999
  • The new approach using the Peak Pattern Method was conducted for the analysis of polychlorinated biphenyls (PCBs) from the papers or paper products to provide certain hygienic data for the recycled papers as well as white papers. The height of the each peak, obtained from the PCBs standards, was transformed to the spectrum to compare with that of the samples. In addition to the results of the single PCBs standards, the pattern of mixed PCBs standards with the adequate concentration and ratios were obtained prior to the analysis of the paper samples. The test showed excellent repetition within 5% variation, and the recoveries of PCBs ranged from 92% to 97%. The PCBs, considered as a hazard material, were analyzed using the Peak Pattern Method from six different types of paper samples including the roll tissue. It could not find the same pattern of the PCBs peaks from all of the paper samples.

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