• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1717-1722
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• 2016

A novel phytase of Acidobacteria was identified from a soil metagenome, cloned, overexpressed, and purified. It has low sequence similarity (<44%) to all the known phytases. At the optimum pH (2.5), the phytase shows an activity level of 1,792 μmol/min/mg at physiological temperature (37℃) and could retain 92% residual activity after 30 min, indicating the phytase is acidophilic and acidostable. However the phytase shows poor stability at high temperatures. To improve its thermal resistance, the enzyme was redesigned using Disulfide by Design 2.0, introducing four additional disulfide bridges. The half-life time of the engineered phytase at 60℃ and 80℃, respectively, is 3.0× and 2.8× longer than the wild-type, and its activity and acidostability are not significantly affected.

• Structural Engineering and Mechanics
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• 제68권4호
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• pp.399-408
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• 2018

Cable supported structures have been widely used in civil engineering. Cable tension estimation has great importance in cable supported structures' analysis, ranging from design to construction and from inspection to maintenance. Even though the Bernoulli-Euler beam element is commonly used in the traditional finite element method for calculation of frequency and cable tension estimation, many elements must be meshed to achieve accurate results, leading to expensive computation. To improve the accuracy and efficiency, a dynamic finite element method for estimation of cable tension is proposed. In this method, following the dynamic stiffness matrix method, frequency-dependent shape functions are adopted to derive the stiffness and mass matrices of an exact beam element that can be used for natural frequency calculation and cable tension estimation. An iterative algorithm is used for the exact beam element to determine both the exact natural frequencies and the cable tension. Illustrative examples show that, compared with the cable tension estimation method using the conventional beam element, the proposed method has a distinct advantage regarding the accuracy and the computational time.

• Journal of Positioning, Navigation, and Timing
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• 제9권3호
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• pp.151-156
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• 2020

A technology for calculating the position of a device is very important for users who receive positioning services, regardless of various indoor/outdoor or with/without any positioning infrastructure existence environments. One of the positioning resources widely used at present, LTE, is a typical infrastructure that can overcome the space limitation, however its positioning method based on the position of the LTE base station has low accuracy. A method of constructing a radio wave map of an LTE signal has been proposed as a method for overcoming the accuracy, but it takes a lot of time and cost to perform high-density collection in a wide area. In this paper, we describe a method of creating a high-density DB for the entire region by using vehicle-based partial collection data. To create a positioning database, we applied the idea of Generative Adversarial Network (GAN), which has recently been in the spotlight in the field of deep learning, and learned the collected data. Then, a virtually generated map which having the smallest error from the actual data is selected as the optimum DB. We verified the effectiveness of the positioning DB generation algorithm using the positioning data obtained from un-collected area.

• 생약학회지
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• 제33권2호통권129호
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• pp.124-129
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• 2002

The effects of Helicobacter pylori and medicinal plants extract (Leweifang) on the viability and interleukin(IL)-8 production of gastric epithelial cell were investigated. Cells viability was significantly decreased when they incubated with H. pylori or H. pylori toxin. Co-incubation with Leweifang increased H. pylori or H. pylori toxin-inhibited cell growth in a concentration-dependent manner. The production of IL-8 was greatly increased in H. pylori-infected KATO III gastric epithelial cells in a concentration- and time-dependent manner. The increased production of IL-8 was significantly inhibited by Leweifang $(1,000{\sim}5,000{\mu}g/ml)$. These results indicate that Leweifang has protective effect on H. pylori-inhibited cell growth and H. pylori-induced gastric mucosal cell inflammation by suppressing the production of inflammatory cytokine (IL-8) from gastric epithelial cells.

• Bulletin of the Korean Chemical Society
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• 제29권7호
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• pp.1386-1390
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• 2008

Artemisinin, the effective ingredient of Chinese herb Artemisia annua L (Qinghao in Chinese), has been proved to be effective to antimalarial. Herein, a reliable, sensitive and convenient electrochemical method was developed for the determination of artemisinin utilizing the excellent properties of multi-wall carbon nanotube (MWNT). The electrochemical behavior of artemisinin was investigated. It is found that the reduction peak current of artemisinin remarkably increases and the peak potential shifts positively by 240 mV at the MWNT film-modified electrode. These phenomena indicate that the MWNT film exhibits efficient catalytic activity to the electrochemical reduction of artemisinin. The effects of pH value, amount of MWNT, scan rate and accumulation time were examined. The limit of detection (S/N = 3) is as low as 10 $\mu$ g $L^{-1}$. Finally, this newly developed method was used to determine the content of artemisinin in Artemisia annua L.

• 동의생리병리학회지
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• 제21권4호
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• pp.815-821
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• 2007

Concept of Cho was used both etiologically and symptomatically at the same time. Hwa was the main cause of diseases derived from Cho, and its damage is on Jin-Eum. Cho is one of the members of Euk-Eum, which belongs to Whae-In, but it is also recognized as pathogen of internal dysfunction, therefore symptoms can be classified into two groups. Whae-cho-zheung and Nae-cho-zheung. Organs related to Cho are lung, stomach, intestine, kidney, liver and gall Bladder. Symptoms of Whae-cho usually combines Pyo-zheung, manifesting on lung and skin, whereas symptoms of Nae-cho combines Rhee-zheung, manifesting on internal organs and tissues. In treating of Cho, Ja-eum-yang-hyul-chung-yeolis the main principle. Chung-Yun-Pe-Weis used in Whae-cho-zheung and Sang-cho-byung, while Yu-Yang-Gan-Shin is used in Nae-cho-zheung and Ha-cho-byung.

• 스마트미디어저널
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• 제8권2호
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• pp.46-57
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• 2019

In recent research, deep learning-based methods have achieved state-of-the-art performance in various computer vision tasks. However, these methods are commonly supervised, and require huge amounts of annotated data to train. Acquisition of data demands an additional costly effort, particularly for the tasks where it becomes challenging to obtain large amounts of data considering the time constraints and the requirement of professional human diligence. In this paper, we present a data level synthetic sampling solution to learn from small and imbalanced data sets using Generative Adversarial Networks (GANs). The reason for using GANs are the challenges posed in various fields to manage with the small datasets and fluctuating amounts of samples per class. As a result, we present an approach that can improve learning with respect to data distributions, reducing the partiality introduced by class imbalance and hence shifting the classification decision boundary towards more accurate results. Our novel method is demonstrated on a small dataset of 2789 tomato plant disease images, highly corrupted with class imbalance in 9 disease categories. Moreover, we evaluate our results in terms of different metrics and compare the quality of these results for distinct classes.

• Genomics & Informatics
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• 제20권1호
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• pp.7.1-7.13
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• 2022

2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.

• 건축역사연구
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• 제31권1호
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• pp.41-50
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• 2022

Traditional wooden buildings deform over time and are vulnerable to fire or earthquakes. Therefore, traditional wooden buildings require continuous management and repair, and securing architectural drawings is essential for repair and restoration. Unlike modernized CAD drawings, traditional wooden building drawings scan and store hand-drawn drawings, and in this process, many noise is included due to damage to the drawing itself. These drawings are digitized, but their utilization is poor due to noise. Difficulties in systematic management of traditional wooden buildings are increasing. Noise removal by existing algorithms has limited drawings that can be applied according to noise characteristics and the performance is not uniform. This study presents deep artificial neural network based noised reduction for architectural drawings. Front/side elevation drawings, floor plans, detail drawings of Korean wooden treasure buildings were considered. First, the noise properties of the architectural drawings were learned with both a cycle generative model and heuristic image fusion methods. Consequently, a noise reduction network was trained through supervised learning using training sets prepared using the noise models. The proposed method provided effective removal of noise without deteriorating fine lines in the architectural drawings and it showed good performance for various noise types.

• 동국한의학연구소논문집
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• 제9권
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• pp.127-138
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• 2000

잠양법(潛陽法)은 평간치법(平肝治法)의 일종으로 간양(肝陽)이 상항(上亢)해서 나타나는 두목혼훈(頭目昏暈), 수족추휵, 구안괘사, 설건어삽(舌乾語澁), 두통불지(頭痛不止), 졸연도지(猝然倒地) 등의 증상(症狀)에 쓰는 치법(治法)이며 중풍(中風)의 표실증(標實證)을 다스리는 목적으로 다용(多用)되고 있다. 중풍(中風)에서의 활용(活用)은 청대(淸代)에 이르러 본격화되었는데, 엽천사(葉天士)는 "자액식풍(滋液熄風), 유양영락(濡養營絡), 보음잠양(補陰潛陽)"을 사용하였고, 장백룡(張伯龍)은 "잠진섭납(潛鎭攝納)"하는 치료법을 사용하였다. 중풍(中風)의 병리(病理)가 본허표실(本虛標實)하므로 항상 자음(滋陰), 보수(補水)하는 치법(治法)과 상호보완적(相互補完的)으로 사용되고 있다. 잠양법(潛陽法)이 다용(多用)된 병증은 간신음허(肝腎陰虛), 풍양상요(風陽上擾)로 인한 중경락증(中經絡證)과 중장부증(中臟腑證) 중에서 양폐증(陽閉證)이며, 탈증(脫證)의 일부와 중풍일구(中風日久)로 인(因)한 전간(癲癎)에도 사용되었다. 잠양락(潛陽樂) 중 진주모(珍珠母), 석결명(石決明), 대모(玳瑁), 모려(牡蠣), 패치(貝齒), 구판(龜板), 별갑(鱉甲) 등 개류(介類)의 효력이 뛰어나고, 치방(治方)으로는 진간식풍탕(鎭肝熄風湯), 호잠환(虎潛丸), 고본단(固本丹), 복맥탕(復脈湯), 천마구등음(天麻鉤藤飮), 영양각탕(羚羊角湯) 등이 활용되고 있다.