• Journal of Apiculture
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• v.32 no.4
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• pp.281-293
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• 2017

Sacbrood virus (SBV) is one of the main pathogenic RNA viruses of honeybee. SBV is found worldwide and many local strains have been reported, such as kSBV, cSBV, and wSBV. In this study, SBV-specific DNA fragments were cloned and sequenced by reverse-transcription PCR from 4 populations of A. mellifera, 4 sequences from 1 population belonged to the 2134D51 genotype (349 nucleotides, nt) and 12 sequences from 3 populations belonged to the 2100D0 genotype (400 nt) among the 16 determined sequences. A total of 87 points of mismatches were found by comparison with the most similar sequences in GenBank. Seventeen single-nucleotide polymorphisms (SNP) were detected, and 6 SNP-patterns in the 2100D0 genotype and 2 SNP-patterns in the 2134D51 genotype were identified based on SNP positions. In SNP-pattern 2, 10 SNPs were detected, but only 2 SNPs were found in SNP-pattern7. Meanwhile, one SNP-pattern was found from one RNA-sample, multi SNP-patterns were detected from other RNA-samples. Large numbers of SNP variants indicate that vast numbers of point-mutations on SBV have occurred since SBV invaded Korea and that SNP smay have been introduced individually over time. Thorough analysis of SNP variants will not only define the local infection-route, but also the relationships between SNP-pattern and SBV-pathogenic abilities.

• Journal of IKEEE
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• v.26 no.3
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• pp.380-388
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• 2022

The traditional malware detection technologies collect known malicious programs and analyze their characteristics. Then such a detection technology makes a blacklist based on the analyzed malicious characteristics and checks programs in the user's system based on the blacklist to determine whether each program is malware. However, such an approach can detect known malicious programs, but responding to unknown or variant malware is challenging. In addition, since such detection technologies generally monitor all programs in the system in real-time, there is a disadvantage that they can degrade the system performance. In order to solve such problems, various methods have been proposed to analyze major behaviors of malicious programs and to respond to them. The main characteristic of ransomware is to access and encrypt the user's file. So, a new approach is to produce the whitelist of programs installed in the user's system and allow the only programs listed on the whitelist to access the user's files. However, although it applies such an approach, attackers can still perform malicious behavior by performing a DLL(Dynamic-Link Library) injection attack on a regular program registered on the whitelist. This paper proposes a method to respond effectively to attacks using DLL injection.

• KIPS Transactions on Software and Data Engineering
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• v.11 no.2
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• pp.87-92
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• 2022

Image-based application testing proposes an application testing method via image structure comparison. This test method allows testing on various devices without relying on various types of device operating systems or GUI. Traditional studies required the creation of a tester for each variant in the existing case, because it differs from the correct image for operating system changes, screen animation execution, and resolution changes. The study determined that the screen is the same for variations. The tester compares the underlying structure of the objects in the two images and extracts the regions in which the differences exist in the images, and compares image similarity as characteristic points of the Faster D2-Net. The development of the Faster D2-Net reduced the number of operations and spatial losses compared to the D2-Net, making it suitable for extracting features from application images and reducing test performance time.

• Archives of Craniofacial Surgery
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• v.24 no.2
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• pp.78-82
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• 2023

Blue nevi, which are characterized by collections of pigment-producing melanocytes in the dermis, have a variety of clinicopathological characteristics. Plaque-type blue nevus (PTBN) is a variant of blue nevi. PTBN presents at birth or arises in early childhood, and it shows a combination of the features found in common blue nevus and cellular blue nevus. It is typically found on the dorsal surface of the hands and feet or on the head and neck, and it is usually benign and stable over time. However, reports have occasionally described malignant melanomas developing in or associated with a PTBN. Malignant blue nevi are most commonly found on the scalp. We report the case of an 88-year-old woman with a malignant melanoma associated with a PTBN of the cheek.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.33 no.3
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• pp.391-399
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• 2023

In this paper, we presents for the first time an implementation using T-table for PIPO-64/128, 256 which are lightweight block ciphers. While our proposed implementation requires 16 T-tables, we show that the two types of T-tables are circulant and obtain variants implementations that require a smaller number of T-tables. We then discuss trade-off between the number of required T-tables (code size) and throughput by evaluating the throughput of the variant implementations on an Intel Core i7-9700K processor. The throughput-optimized versions for PIPO-64/128, 256 provide better throughput than TLU(Table-Look-Up) reference implementation by factors of 3.11 and 2.76, respectively, and bit-slice reference implementation by factors of 3.11 and 2.76, respectively.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.5C
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• pp.359-368
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• 2006

After construction, time-variant seepage and long-term underground motion are representative factors to understand the abnormal behavior of tunnels. In this study, numerical models have been developed to analyze the behavior of tunnels associated with seepage and long-term underground motion. Possible scenarios have been investigated to establish causes-and-results mechanisms. Various parameters such as permeability of tunnel filter, seepage condition, water table, long-term rock mass load, size of damaged zone due to excessive blasting have been investigated. These are divided into two sub-parts depending on the tunnel type and major loading mechanisms depending on the types. For the soft ground tunnels, the behavior associated with seepage conditions has been studied and the effect of permeability change in tunnel-filter and the effect of water-table change which are seldom measurable are investigated in detail. For the rock mass tunnels, tunnel behavior associated with the visco-plastic behavior of rock mass has been studied and the long-term rock mass loads as a result of relaxation and creep have been considered.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.28-30
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• 2022

Recently, the number of confirmed cases has increased again with the new variant of COVID-19. Quarantine is recommended, especially to prevent the rapidly increasing spread, as environmental controls, such as minimizing contact with others, can increase safety. In addition, there are often cases in which the patient's condition cannot be confirmed from the standpoint of a guardian, such as visitation being prohibited under certain conditions. At this time, the sensor data values of oxygen, carbon dioxide concentrations, temperature and humidity, and alcohol, which are medical gases used in hospitals, are collected remotely using ZigBee wireless communication technology. Design a system that can be stored and monitored in a database. We propose an environmental monitoring system, which is a visualization system designed to allow hospitals to check and feedback data on the managed environment, and to give reliability to parents.

• Journal of Plant Biotechnology
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• v.50
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• pp.190-199
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• 2023

Date palm (Phoenix dactylifera L.: Arecaceae) is a dioecious species where only female trees bear fruits. In their natural state, date palms produce dates once a year. However, in Thailand, some trees were observed to produce dates during the off-season, despite no variations in morphology. The availability of such off-season fruits can significantly increase their market value. Interestingly, most female off-season date palms investigated in this study were obtained through micropropagation. Hence, there is an urgent need for genetic markers to distinguish female offseason flowering plantlets within tissue culture systems. In this study, we aimed to develop random amplification of polymorphic DNA-sequence characterized amplified region (RAPD-SCAR) markers for the identification of female off-season flowering date palms cultivated in Thailand. A total of 160 random decamer primers were employed to screen for specific RAPD markers in off-season flowering male and female populations. Out of these, only one primer, OPN-02, generated distinct genomic DNA patterns in female off-season flowering (FOFdp) individuals compared to female seasonal flowering genotypes. Based on the RAPD-specific sequence, specific SCAR primers denoted as FOFdpF and FOFdpR were developed. These SCAR primers amplified a single 517-bp DNA fragment, predominantly found in off-season flowering populations, with an accuracy rate of 60%. These findings underscore the potential of SCAR marker technology for tracking offseason flowering in date palms. Notably, a BLAST analysis revealed a substantial similarity between the SCAR marker sequence and the transcript variant mRNA from Phoenix dactylifera encoding the SET DOMAIN GROUP 40 protein. In Arabidopsis, this protein is involved in the epigenetic regulation of flowering time. The genetic potential of the off-season flowering traits warrants further elucidation.

• Applied Microscopy
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• v.51
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• pp.4.1-4.12
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• 2021

Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.

• Journal of Life Science
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• v.20 no.4
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• pp.511-518
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• 2010

Exercise-induced anaphylaxis (EIA) is defined as the onset of allergic symptoms during, or immediately after, exercise, the clinical signs being various degrees of urticaria, angioedema, respiratory and gastrointestinal signs, and even anaphylactic shock. Food-dependent exercise-induced anaphylaxis (FDEIA) is a specific variant of exercise-induced anaphylaxis that requires both vigorous physical activity and the ingestion of specific foods within the preceding several hours. To describe the physiopathologic mechanism, etiologic factors, and clinical manifestations, we evaluated the supplementation of vitamin C and catalase on spleen index, proliferation assay, ROS, and ASAS in sensitized and exercise trained mice. The results were as follows: Spleen index showed the highest level in the ST12 group compared to other groups; this level increased in a time dependent manner and in significant amounts. In proliferation assay of Med and OVA, the ST12 group showed the highest level compared to other groups; this level also increased in a time dependent manner. On the other hand, spleen ROS did not show a statistically significant difference, and peritoneal ROS showed the highest level in the ST12 group. ASAS showed the highest level in the ST12 compared to the S; this was also in a time dependent manner and in significant amounts. From the results, we chose the ST9 and ST12 groups to evaluate allergy anaphylaxis with supplementation of Vitamin C and catalase. In both the ST9 and ST12 groups, peritoneal ROS and ASAS were lower in vitamin C treatment group than in the catalase treatment group. This was a statistically significant difference. From the results, allergy anaphylaxis showed a higher level in the long trained group than in the short trained group. Also, treatment with vitamin C was more effective in lowering allergy anaphylaxis than catalase treatment.