• Journal of Intelligence and Information Systems
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• v.15 no.4
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• pp.123-140
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• 2009

Fuzzy neural network is an integrated model of artificial neural network and fuzzy system and it has been successfully applied in control and forecasting area. Recently ANFIS(Adaptive Network-based Fuzzy Inference System) has been noticed widely among various fuzzy neural network models because of outstanding performance of control and forecasting accuracy. ANFIS has capability to refine its fuzzy rules interactively with human expert. In particular, when we use initial rule structure for machine learning which is generated from human expert, it is highly probable to reach global optimum solution as well as shorten time to convergence. We propose metrics to evaluate interpretability of generated rules as a means of acquiring domain knowledge and compare level of interpretability of ANFIS fuzzy rules to those of C5.0 classification rules. The proposed metrics also can be used to evaluate capability of rule generation for the various machine learning methods.

• Journal of the Korean Association of Geographic Information Studies
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• v.7 no.3
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• pp.35-47
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• 2004

Telematics and LBS is one of rapidly emerged technology domains. In order to efficiently construct them, moving object technology which manages huge volume of real-time location data is required. Especially, the query which obtains special sorts of information closely related to the detailed applications is required in order to effectively retrieve and analyze the location data for moving object in logistics domain. It has also complex query structure comparing to the conventional database query. The approach using the standard database query language, like SQL, can be considered as an effective alternative choice. In this paper, we not only propose a new query language, entitled as MOQL based on SQL, for the query processing of the vehicle monitoring and control in e-Logistics but also design and implement the query processor.

• Journal of the Korea Convergence Society
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• v.8 no.5
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• pp.1-11
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• 2017

With a given spring-mass-damper system, $H_{\infty}$ and ${\mu}$-synthesis control methods are used to build system controllers which minimize vibrations at two major natural frequencies in two cases; without uncertainty; with 20% uncertainty. In order to check the stability and performance of two controllers, those are examined using GM and PM values. The signal strength of output responses is compared using the concept of central numerical differentiation and then results are quantified using the RMS method. Lastly, 40 random samples of $H_{\infty}$ and ${\mu}$-synthesis controllers are obtained for three different $W_{per\;f1}$ weighting functions and drawn in the time domain in order to compare the stability. Overall, ${\mu}$-synthesis controller manages the vibrations much better than $H_{\infty}$ controller according to the robust stability and performance values obtained by simulating random samples of 40 plant models.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.1 s.104
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• pp.74-80
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• 2006

This paper presents the characteristics of electromagnetic field uniformity in a reverberation chamber that can be used alternatively for the analysis, test and evaluation of electromagnetic interference and immunity. The dual-band diffuser, which can be used at two different frequency bands in a reverberation chamber, is applied, and it is made of two different single-band Schroeder's Quadratic Residue Diffusers. The FDTD method is used to analyze the field characteristics. Compared with single-band diffuser, the dual-band diffuser shows the improvement in not only expansion of test frequency band but also in characteristics of the field uniformity, polarity, power efficiency, and tolerance. Therefore, the reverberation chamber with the dual-band diffuser is a better facility, for the analysis and measurement of electromagnetic interference and immunity, than the reverberation chamber with a single-band diffuser.

• Molecules and Cells
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• v.43 no.4
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• pp.373-383
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• 2020

Our previous study revealed a novel role of Fas-associated death domain-containing protein (FADD) in islet development and insulin secretion. Insulin-degrading enzyme (IDE) is a zinc metalloprotease that selectively degrades biologically important substrates associated with type 2 diabetes (T2DM). The current study was designed to investigate the effect of FADD phosphorylation on IDE. We found that the mRNA and protein levels of IDE were significantly downregulated in FADD-D mouse livers compared with control mice. Quantitative real-time polymerase chain reaction analysis showed that FADD regulates the expression of IDE at the transcriptional level without affecting the stability of the mRNA in HepG2 cells. Following treatment with cycloheximide, the IDE protein degradation rate was found to be increased in both FADD-D primary hepatocytes and FADD-knockdown HepG2 cells. Additionally, IDE expression levels were reduced in insulin-stimulated primary hepatocytes from FADD-D mice compared to those from control mice. Moreover, FADD phosphorylation promotes nuclear translocation of FoxO1, thus inhibiting the transcriptional activity of the IDE promoter. Together, these findings imply a novel role of FADD in the reduction of protein stability and expression levels of IDE.

• Journal of Animal Science and Technology
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• v.54 no.3
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• pp.157-163
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• 2012

The male reproduction is precisely controlled by a number of intrinsic and extrinsic factors. These factors usually involve in expressional regulation of various molecules influencing on sperm production in the testis. A number of ways are employed to control the transcription of specific genes, including epigenetic modifications of DNA and histone molecules. DNA methylation of CpG dinucleotides is a commonly used regulatory mechanism for testicular genes associated with the fertility. Previous studies have demonstrated the infertility induced by improper DNA methylation of these genes. In the present research, we attempted to determine transcriptional expression of some of these genes in the rat testis at different postnatal ages using real-time PCR analysis. These genes include neurotrophin 3 (Ntf3), insulin-like growth factor II (Igf2), JmjC-domain-containing histone demethylase 2A 1 (Jhm2da), paired box 8 transcription factor (Pax8), small nuclear ribonucleoprotein polypeptide N (Snrpn), and 5,10-methylenetetrahydrofolate reductase (Mthfr). The expression levels of Ntf3, Igf2, and Snrpn genes were the highest at the neonatal age, followed by transient decreases at the prepubertal age. Expression of Jhm2da and Mthfr genes were continuously increased from the neonate to 1 year of age. The levels of Pax8 mRNA at the early ages were higher than those at the later ages of postnatal development. These findings suggest that expression of some fertility-associated testicular genes in the rat during postnatal period could be differentially regulated by the control of the degree of DNA methylation.

• KSBB Journal
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• v.22 no.4
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• pp.260-264
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• 2007

Peptides are frequently studied as candidates for new drug development. Recently, synthesized peptide library is screened for a certain functionality on a microarray biochip format. In this study, in order to replace the conventional cellulose membrane with glass for a microarray chip substrate for peptide library screening, we modified the glass surface from amines to thiols and covalently immobilized the peptides. Using trypsin-FITC (fluorescein isothiocyanate) conjugate that could specifically bind to a trypsin binding domain consisting of a 7-amino acid peptide, we checked the degree of surface modification. Because of the relatively lower hydrophilicity and reduced surface roughness, the conjugation reaction to the glass required a longer reaction time and a higher temperature. It took approximately 12 hr for the reaction to be completed. From the fluorescence signal intensity, we could differentiate between the target and the control peptides. This difference was confirmed by a separate experiment using QCM. Furthermore, a smaller volume and higher concentration of a spot showed a higher fluorescence intensity. These data would provide the basic conditions for the development of microarray peptide biochips.

• Animal cells and systems
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• v.16 no.4
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• pp.280-288
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• 2012

Targeted degradation of proteins through ubiquitin-mediated proteolysis is an important control mechanism in various cellular processes. The process of ubiquitin conjugation is achieved by three enzyme complexes, among which the ubiquitin ligase complex (E3) is in charge of substrate specificity. The SCF (SKP1-CUL1-F-box) family portrays the largest and the most characterized member of the E3 ligases. For each SCF complex, the ubiquitination target is recognized by the F-box protein subunit, which interacts with the substrate through a unique C-terminal domain. We have characterized a novel F-box protein CFL-1 that represents a single LRR-type F-box (FBXL) in the Caenorhabditis elegans genome. CFL-1 is highly homologous to FBXL20 and FBXL2 of mammals, which are known to regulate synaptic vesicle release and cell cycle, respectively. A green fluorescence protein (GFP)-reporter gene fused to the cfl-1 promoter showed restricted expression around the amphid and the anus. Modulation of CFL-1 activity by RNAi affected the time interval between defecations. RNAi-treated worms also exhibited reduced tendency to form dauer when exposed to daumone. The potential involvement of CFL-1 in the control of defecation and pheromone response adds to the ever expanding list of cellular processes controlled by ubiquitin-mediated proteolysis in C. elegans. We suggest that CFL-1, as a single LRR-type F-box protein in C. elegans, may portray a prototype gene exerting diverse functions that are allocated among multiple FBXLs in higher organisms.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.5 s.120
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• pp.471-477
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• 2007

This paper presents the characteristics of electromagnetic field uniformity in a reverberation chamber with flat diffusers composed of multiple dielectric materials that can be used as an alternative facility for the analysis, test and evaluation of electromagnetic interference and immunity. The field characteristics and the size of the test volume inside the reverberation chamber with the new diffuser of multiple dielectric materials are simulated and analyzed at $2.5{\sim}3$ GHz band. The FDTD method is used to analyze the field characteristics. The field uniformity, polarization characteristics and tolerance are improved by the new diffuser with smaller physical size. The reverberation chamber with flat diffusers composed of multiple dielectric materials shows better electromagnetic performance and larger test volume than normal chamber.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.5
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• pp.959-967
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• 1988

In this paper, 2-DOF vehicle suspension system with a semiactive on-off damper was studied for improving the ride comfort. It is known that a nonlinear hydraulic damper, which generates force proportional to the square of the relative velocity, can describe the actual fluid resisting type damper more properly than the traditional viscous damping model. On the other hand, hydraulic damper adoption in analysis makes the system nonlinear and causes difficulties to get the system response. In this work, time domain direct integration method was used to calculate system displacement and acceleration. first of all, the response of the suspension system experiencing a given road profile was optimized by Lagrangian multiplier method within the range of given damping coefficients. The appropriate on-loaf damping values were obtained by averaging the already calculated optimum damping coefficients from Lagrangian techniques. The criterion to control the on-off mechanism was determined by examining the suspension efficiency. It was found that the best out of practically applicable criteria is following the sign (positive and negative) of the multiplication of relative displacement and velocity. Judging from the theoretical calculations, it was proved that the semiactive on-off damper can increase suspension efficiency as much as 8-11% in object function.