• 한국식생활문화학회지
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• 제33권5호
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• pp.464-471
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• 2018

Purpose: This study examined the effects of ${\alpha}$-lipoic acid in diluted solvents on cell growth in 3T3-L1 cells according to the treated concentration and times. Methods: Adipocyte 3T3-L1 cell were cultured. Confluent cells underwent starvation with SFM for 1 day and then were cultured in a medium containing various concentrations 0, 100, 200, and $400{\mu}mol/L$ of ${\alpha}$-lipoic acid. The cell viability was measured using the EZ Cytox assay kit. In addition, the effect of ${\alpha}$-lipoic acid of diluted solvents on the cell growth in 3T3-L1cells was examined according to the treated concentration and times. Results: The ${\alpha}$-lipoic acid diluted ethanol inhibited cell proliferation in a dose and time dependent manner. The ${\alpha}$-lipoic acid diluted ethanol induced adipocyte 3T3-L1 cells proliferation with an adipocyte inducer. In addition, ${\alpha}$-lipoic acid inhibited adipocyte 3T3-L1 growth in a dose and time dependent manner (p<0.05). Conclusion: This study showed that a treatment with ${\alpha}$-lipoic acid diluted ethanol inhibits cell growth of, adipocyte 3T3-L1 cells induced with an adipocyte inducer, ($200{\mu}mol/L$ of ${\alpha}$-lipoic acid) treated for 48 hr.

• 대한토목학회논문집
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• 제28권1A호
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• pp.15-24
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• 2008

본 논문에서는 프리캐스트 바닥판을 사용한 단순 강합성보에 교축방향으로 프리스트레스를 도입하는 경우, 콘크리트의 장기거동과 PS강재의 릴렉세이션에 의해 발생하는 프리스트레스의 손실과 장기처짐을 계산하기 위한 장기거동해석방법을 제시하였다. 또한 제시된 해석방법을 이용하여 장기거동해석을 수행하였으며, 이를 통해 합성보의 기하형상, 콘크리트강도 및 초기프리스트레스가 프리캐스트 바닥판을 사용한 강합성보의 장기거동에 미치는 영향을 평가하였다. 해석결과 합성보 기하형상의 영향에서 프리스트레스의 손실과 장기처짐에 영향을 미치는 주요인자는 각각 보의 단면적과 단면2차모멘트였다. 마지막으로, 총 프리스트레스 손실에서 크리프에 의한 손실과 건조수축에 의한 손실의 특성을 분석하여 소요 초기프리스트레스의 산정방법을 제안하였다.

• KSBB Journal
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• 제27권5호
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• pp.289-294
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• 2012

Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.

• Journal of Nutrition and Health
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• 제54권4호
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• pp.335-341
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• 2021

Purpose: Muscle mitochondria play a key role in regulating fatty acid and glucose metabolism. Dysfunction of muscle mitochondria is associated with metabolic diseases such as obesity and type 2 diabetes. Isorhamnetin (ISOR), also known as 3-O-methylquercetin, a quercetin metabolite, is a naturally occurring flavonoid in many plants. This study evaluated the effects of ISOR on the regulation of the mitochondrial function of C2C12 muscle cells. Methods: C2C12 muscle cells were differentiated for 5 days, and then treated in various concentrations of ISOR. Cytotoxicity was determined by assessing cell viability using the water-soluble tetrazolium salt-8 assay principle at different concentrations of ISOR and time points. Levels of the mitochondrial DNA (mtDNA) content and gene expression were measured by quantitative real-time polymerase chain reaction. The citrate synthase (CS) activity was quantified by the enzymatic method. Results: ISOR at a concentration of 10 µM did not show any cytotoxic effects. ISOR increased the mtDNA copy number in a time- or dose-dependent manner. The messenger RNA levels of genes involved in mitochondrial function, such as peroxisome proliferator-activated receptor-γ coactivator-1α, and uncoupling protein 3 were significantly stimulated by the ISOR treatment. The CS activity was also significantly increased in a time- or dose-dependent manner. Conclusion: These results suggest that ISOR enhances the regulation of mitochondrial function, which was at least partially mediated via the stimulation of the mtDNA replication, mitochondrial gene expression, and CS activity in C2C12 muscle cells. Therefore, ISOR may be useful as a potential food ingredient to prevent metabolic diseases-associated muscle mitochondrial dysfunction.

• 한국치위생학회지
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• 제10권3호
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• pp.531-540
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• 2010

Objectives : In this study, following the cavity restorations with low copper conventional alloy, high copper admixed one and high copper unicompositional one, which are used the most frequently in a clinical setting at the present, to experiment the time-dependent changes of strength, bubbles were examined. Besides, to examine the detrimental effects of mercury contained in dental amalgam, the amount of mercury release was evaluated. Methods : As dental amalgams which were used herein, [BESTALOY], [Hi-Aristaloy 21] and [Sybraloy] were selected for a low-copper conventional amalgam, a high-copper admixed one and a high-copper unicompositional one in the corresponding order. The formation of bubbles and the weight ratio of mercury release were evaluated using a field emission scanning electron microscope (FE-SEM). Thus, the following results were obtained: Results : 1. The time-dependent amount of mercury release reached a statistical significance in three types of alloys, which was shown in such a descending order as [BESTALOY], [Hi-Aristaloy 21] and [Sybraloy]. 2. A low-copper conventional type, BESTALOY is a cutting type and it was found to have an increased formation of fine bubbles. In the remaining two types, [Hi-Aristaloy 21] (a high-copper admixed alloy) and [Sybraloy] (a high-copper unicompositional alloy), the time-dependent changes in the formation of bubbles was negligible. Conclusions : Accordingly, this type of mercury release from amalgam alloy denotes the difference in the weight ratio of total constituents between after 24 hours and after two weeks. But further studies are warranted to examine the amount of mercury release which is detrimental to human bodies. Besides, a low-copper conventional alloy is a cutting type and it was characterized by the abundant formation of bubbles in a time-dependent manner. This implies that the strength of amalgam is impaired, which should be considered in selecting the appropriate amalgam alloy in a clinical setting.

• Steel and Composite Structures
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• 제17권4호
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• pp.405-429
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• 2014

In this paper, it is aimed to determine the structural behavior of suspension bridges considering construction stages and different soil conditions. Bosporus Suspension Bridge connecting the Europe and Asia in Istanbul is selected as an example. Finite element model of the bridge is constituted using SAP2000 program considering existing drawings. Geometric nonlinearities are taken into consideration in the analysis using P-Delta large displacement criterion. The time dependent material strength of steel and concrete and geometric variations is included in the analysis. Time dependent material properties are considered as compressive strength, aging, shrinkage and creep for concrete, and relaxation for steel. To emphases the soil condition effect on the structural behavior of suspension bridges, each of hard, medium and soft soils are considered in the analysis. The structural behavior of the bridge at different construction stages and different soil conditions has been examined. Two different finite element analyses with and without construction stages are carried out and results are compared with each other. At the end of the analyses, variation of the displacement and internal forces such as bending moment, axial forces and shear forces for bridge deck and towers are given in detail. Also, displacement and stresses for bridge foundation are given with detail. It can be seen from the analyses that there are some differences between both analyses (with and without construction stages) and the results obtained from the construction stages are bigger. It can be stated that the analysis without construction stages cannot give the reliable solutions. In addition, soil condition have effect on the structural behavior of the bridge. So, it is thought that construction stage analysis using time dependent material properties, geometric nonlinearity and soil conditions effects should be considered in order to obtain more realistic structural behavior of suspension bridges.

• 콘크리트학회논문집
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• 제18권6호
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• pp.711-720
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• 2006

FCM 교량 상부구조의 거동은 콘크리트의 크리프와 건조수축 등에 의해 시간 의존적 거동을 나타내며, 이에 의해 하부구조 즉, 교각에는 시간에 따른 추가의 과도한 모멘트가 발생할 수 있다. 본 연구에서는 FCM 공법으로 시공되는 콘크리트 교량의 크리프와 건조수축에 의한 교각의 모멘트를 감소시킬 수 있는 기법을 제안하였다. 제안 기법은 교량의 체계적인 시간의존적 거동 해석과 교량 시공중의 제어하중 재하로 이루어진다. 제어하중의 크기 및 교각 유연도에 따른 교각의 거동 분석을 수행하였다. 교각 유연도에 따른 수치해석 결과는 교각의 유연도가 작을수록 시간에 따른 교각 모멘트 값은 증가하여 설계시 불리한 측면을 나타내고 있다. 제어하중을 도입하지 않은 경우에 비해 제어하중을 도입한 경우의 모멘트는 감소하고 있다. 또한, 실제 교량에 대한 수치해석 분석을 통하여 제안기법의 효용성을 제시하였다. 따라서, FCM 공법으로 시공되는 교량에서, 유연도가 작은 교각의 시간에 따른 모멘트 변화를 효율적으로 제어할 수 있다고 판단된다.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2000년도 제31회 춘계학술대회
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• pp.114-120
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• 2000

EHL analysis of connecting rod bearing is proposed which includes effects of temperature variation in lubrication film. Lubrication film temperature is treated as a time-dependent, two-dimensional variable which is averaged over the film thickness, while connecting rod big end temperature is assumed to be time-independent and three-dimensional. It is assumed that a portion of the heat generated by viscous dissipation in the lubrication Him is absorbed by the film itself, and the remainder flows into the bearing surface. Mass-conserving cavitation algorithm is applied and the effect of variable viscosity is included to solve the Reynolds equation. Simulation results of the connecting rod bearing of internal combustion engine are presented. It is shown that the temperature variation has remarkable effects on the bearing performance. It is concluded that the EHL analysis considering effects of the temperature variation is strongly recommended to predict the bearing performance of connecting rod big end On internal combustion engine.

• Tribology and Lubricants
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• 제17권3호
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• pp.228-235
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• 2001

EHL analysis of connecting rod bearing is proposed which includes effects of temperature variation in lubrication film. Lubrication film temperature is treated as a time-dependent, two-dimensional variable which is averaged over the film thickness, while connecting rod big end temperature is assumed to be time-independent and three-dimensional. It is assumed that a portion of the heat generated by viscous dissipation in the lubrication film is absorbed by the film itself, and the remainder flows into the bearing surface. Mass-conserving cavitation algorithm is applied and the effect of variable viscosity is included to solve the Reynolds equation. Simulation results of the connecting rod bearing in internal combustion engine are presented. It is shown that the temperature variation has remarkable effects on the bearing performance. It is concluded that the EHL analysis considering effects of the temperature variation is strongly recommended to predict the connecting rod bearing performance in internal combustion engine.

• 산업경영시스템학회지
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• 제13권21호
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• pp.91-100
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• 1990

This paper is concerned with the development of optimal algorithms for multi-stage flowshop scheduling problems with sequence dependent setup times. In the previous researches the setup time of a job is considered to be able to begin at the earliest opportunity given a particular sequence at the start of operations. In this paper the setup time of a job is considered to be able to begin only at the completion of that job on the previous machine to reflect the effects of the setup time to the performance measure of sequence dependent setup time flowshop scheduling. The results of the study consist of two areas; first, a general integer programming(IP) model is formulated and a nixed integer linear programming(MILP) model is also formulated by introducing a new binary variable. Second a depth-first branch and bound algorithm is developed. To reduce the computational burdens we use the best heuristic schedule developed by Choi(1989) as the first trial. The experiments for developed algorithm are designed for a 4$\times$3$\times$3 factorial design with 360 observations. The experimental factors are PS(ratio of processing time to setup time), M(number of machines), N(number of jobs).