• Journal of Soil and Groundwater Environment
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• v.11 no.5
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• pp.43-50
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• 2006

Bioreporter bacteria, such as recombinant bioluminescent bacteria, have been used for the detection of specific compounds in complex environmental media. In this study, optimum conditions for the preparation and application of deep-freezed and Iyophilized recombinant bioluminescent strain KG1206 were investigated for the future application on contaminated environmental sites. Genetically engineered microorganism, Pseudomonas putida mt-2 KG1206, contains TOL plasmid and the plasmid inserted $P_{m}$, promoter on the upper part of lux gone in vector pUCD615, and m-toluate and benzoate are considered direct inducers for bioluminescence. Optimum conditions determined for the preparation and application of the deep-freezed and lyophilized strain were followings: cryoprotective agent (24% sucrose), lyophilization time (12 hrs), strain concentration ($OD_{600}=0.6$), reconstitution for freezed strain (quick reconstitution at $35^{\circ}C$), reconstitution for lyophilized strain ($3{\sim}6$ hrs exposure on LB medium), carrying conditions (keep at $20^{\circ}C$ after reconstitution). These results demonstrate the feasibility of deep-freezed or lyophilized state of genetically engineered bioluminescent strain for environmental usage.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.380-386
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• 1998

To obtain basic data for mass production of Isaria japonica, cultural characteristics of japonica were investigated by using liquid, solid media and silkworms pupa. Mycelia grew favorably at the temperature of $23{\sim}28^{\circ}C$ on MYG medium with pH 7.0. The fruiting-body of I. japonica was induced below $20^{\circ}C$ in MYG liquid medium (Malt yeast glucose) under fluorescent light. In MYG basal medium mixed with pupal powder of silkworms, the fresh weight of fruiting-bodies was increased with increasing concentration of pupal powder. The highest yield of fruiting bodies was obtained in carbon-rich medium supplemented with pupal powder of silkworm. Also, fruiting-bodies of I. japonica were produced massively on the silkworm pupa placed on the stainless tray in the shortest time. The structure and shape of fruiting-bodies were coral-like, many-branched types with numerous conidiospores.

• Journal of Chitin and Chitosan
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• v.23 no.4
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• pp.285-292
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• 2018

In this study, for the purpose of decomposing food waste, the strain was screened from traditional fermented food and soils. The enzyme activity (protease, amylase, cellulase, lipase) experiment was carried out using the paper disc method in 212 strains isolated from 5% NaCl media. Among them, only the strains having enzyme activity of more than 2 (soil) or more than 4 (traditional fermented food) with the halozone of enzyme activity of 15 mm or more were selected first, and microorganism identification through 16S rRNA sequencing was performed. Finally, were identified such as Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus siamensis, Bacillus licheniformis, Bacillus aquimaris, Bacillus megaterium, Bacillus koreensis, Bacillus stratoshericus, Bacillus aryabhattai, Bacillus safensis, Marinobacter hydrocarbonoclasticus. 11 species of mixed strains were confirmed that the culture time was 24 hours, the incubation temperature was $30^{\circ}C$ and the optimum pH was 7.0. In order to confirm the degree of decomposition of standard food wastes (100 g) by treating 11 kinds of mixed strains (25%), solid content of more than $2000{\mu}m$ was determined to be 103 g for the sterilized water group and 18 g for the mixed strains group. And the rest was decomposed to a size of less than $2000{\mu}m$.

• International Journal of Advanced Culture Technology
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• v.9 no.4
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• pp.18-28
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• 2021

This study surveyed how retirees' evaluation of starting food service business affects the effectiveness their new business and quality of life, based on personal factors such as entrepreneurship and business-starting capability and environmental factors by using questionnaires. Bootstrapping was carried out in order to find out factors affecting rapidly changing new business environments and retirees' initial intent to start a business so as to verify basic hypothesis about relation between retirees' evaluation of starting food service business (social, economic, and psychological effects) and the effectiveness of their new business and quality of life and confirm whether the effectiveness of the new business acts as a medium between the evaluation of starting food service business and quality of life. In addition, PLS-MGA was performed in order to verify whether the correlations among test factors can be varied according to the kind of job the target retirees had. Having examined the basic thesis, it was found that social and economic factors had significant positive effect on the effectiveness of the new business, and social and psychological factors had significant positive effect on the quality of life. Having analyzed whether the effectiveness of new business acted as a media between evaluation of starting food service business and quality of life, there was no significant effect as a medium. Having studied whether the kind of job of the retirees controlled or affected the relations among evaluation of starting food service business, effectiveness of new business and quality of life, the results were as follows: in the office job retiree group, the greater economic factor led to increase of effectiveness of new business, while social and psychological factors influenced the quality of life; In the physical labor group, the higher social factor resulted in higher effectiveness of new business, which showed significant positive effect on the quality of life. Having researched about which element is considered to be most important in starting food service business, the most important element was found to be dish/menu, followed by staff management, accounting management, business management, and service education. Having analyzed relation between accomplishment and important consideration for starting food service business, "managers with entrepreneurship," "appropriate distribution of time to prepare for starting business," and "operation of practical field education programs" showed higher importance compared to the degree of satisfaction, so it is needed to more concentrate on the above matters. This study intends to raise retirees' awareness of starting business and help them live better life based on the analysis results, and further suggest detailed mechanism and specific operations of factors affecting retirees' decision making on starting business, such that they can use the information as basic materials to make better choices that can lead to successful business.

• Journal of Broadcast Engineering
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• v.26 no.4
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• pp.409-418
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• 2021

Conformal coating is a technology that protects PCB(Printed Circuit Board) and minimizes PCB failures. Since the defects in the coating are linked to failure of the PCB, the coating surface is examined for air bubbles to satisfy the successful conditions of the conformal coating. In this paper, we propose an algorithm for detecting problematic bubbles in high-risk groups by applying image signal processing. The algorithm consists of finding candidates for problematic bubbles and verifying candidates. Bubbles do not appear in visible light images, but can be visually distinguished from UV(Ultra Violet) light sources. In particular the center of the problematic bubble is dark in brightness and the border is high in brightness. In the paper, these brightness characteristics are called valley and mountain features, and the areas where both characteristics appear at the same time are candidates for problematic bubbles. However, it is necessary to verify candidates because there may be candidates who are not bubbles. In the candidate verification phase, we used convolutional neural network models, and ResNet performed best compared to other models. The algorithms presented in this paper showed the performance of precision 0.805, recall 0.763, and f1-score 0.767, and these results show sufficient potential for bubble test automation.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.197-202
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• 2019

In the era of the fourth revolution, interest in content production using proven engines in the broadcasting sector, such as Vizrt, is growing. The new visual effects required in the 5G era are critical to content production training. Vizrt has a good production time utility and affordability for broadcast and media content. In this paper, we are going to use this to present a practical case of the theorem and application of the basic training course in the production of virtual content, and to present the basic training direction. In the introduction, the graphic algorithm analyzed and studied the characteristics and environmental factors of the Vizrt engine. In this paper, the production process was studied separately, and the work carried out through engine implementation was presented. The VS Studio Foundation was provided as a practical production case at each stage. The Vizrt engine operator process is important in graphic approach and application, and through the results of the lecture, the method of understanding and implementing algorithms for virtual reality perspective suitable for basic learning was studied. Based on practice, the research method of main theory was to create Vizrt contents specialized in 5G contents work in each sector and to implement graphic production in new areas from contents image. Through this study, we came to the conclusion of the basic training method through virtual reality content work based on Vizrt by practicing content creation according to the subject. It also proposes the effect of creating Vizrt content and the direction of building Vizrt basic training courses.

• Journal of the Korean Society for information Management
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• v.36 no.3
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• pp.149-174
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• 2019

This study analyzes the actual use data of the websites of university libraries, analyzes the users' usage behavior, and proposes improvement measures for the websites. The study analyzed users' traffic and analyzed their usage behavior from January 2018 to December 2018 on the C University website. The website's analysis tool used 'Google Analytics'. The web traffic variables were analyzed in five categories: user general characteristics, user environment analysis, visit analysis, inflow analysis, site analysis, and site analysis based on the metrics of sessions, users, page views, pages per session, average session time, and bounce rate. As a result, 1) In the analysis results of general characteristics of users, there was some access to the website not only in Korea but also in China. 2) In the user experience analysis, the main browser type appeared as Internet Explorer. The next place was Chrome, with a bounce rate of Safari, third and fourth, double that of the Explore or Chrome. In terms of screen resolution, 1920x1080 resolution accounted for the largest percentage, with access in a variety of other environments. 3) Direct inflow was the highest in the inflow media analysis. 4) The site analysis showed the most page views out of 4,534,084 pages, followed by the main page, followed by the lending/extension/history/booking page, the academic DB page, and the collection page.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.17 no.3
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• pp.329-338
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• 2019

Suction of unsaturated bentonite buffers is a very important input parameter for hydro-mechanical performance assessment and design of an engineered barrier system. This study analyzed suction measurement techniques and constitutive models of unsaturated porous media reported in the literature, and suggested suction measurement techniques and constitutive models suitable for bentonite buffer in an HLW repository. The literature review showed the suction of bentonite buffer to be much higher than that of soil, as measured by total suction including matric suction and osmotic suction. The measurement methods (RH-Cell, RH-Cell/Sensor) using a relative humidity sensor were suitable for suction measurement of the bentonite buffer; the RH-Cell /Sensor method was more preferred in consideration of the temperature change due to radioactive decay heat and measurement time. Various water retention models of bentonite buffers have been proposed through experiments, but the van Genuchten model is mainly used as a constitutive model of hydro-mechanical performance assessment of unsaturated buffers. The water characteristic curve of bentonite buffers showed different tendencies according to bentonite type, dry density, temperature, salinity, sample state and hysteresis. Selection of water retention models and determination of model input parameters should consider the effects of these controlling factors so as to improve overall reliability.

• Journal of Broadcast Engineering
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• v.24 no.3
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• pp.495-505
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• 2019

Typical algorithms of deep learning include CNN(Convolutional Neural Networks), which are mainly used for image recognition, and RNN(Recurrent Neural Networks), which are used mainly for speech recognition and natural language processing. Among them, CNN is able to learn from filters that generate feature maps with algorithms that automatically learn features from data, making it mainstream with excellent performance in image recognition. Since then, various algorithms such as R-CNN and others have appeared in object detection to improve performance of CNN, and algorithms such as YOLO(You Only Look Once) and SSD(Single Shot Multi-box Detector) have been proposed recently. However, since these deep learning-based detection algorithms determine the success of the detection in the still images, stable object tracking and detection in the video requires separate tracking capabilities. Therefore, this paper proposes a method of combining Kalman filters into deep learning-based detection networks for improved object tracking and detection performance in the video. The detection network used YOLO v2, which is capable of real-time processing, and the proposed method resulted in 7.7% IoU performance improvement over the existing YOLO v2 network and 20 fps processing speed in FHD images.

• The Journal of the Korea Contents Association
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• v.21 no.10
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• pp.286-308
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• 2021

For a long time, dramas that everyone has enjoyed at home have become the most popular cultural contents due to the development of digital technology and the influence of Hallyu.(Korean Wave) This study was conducted in-depth interviews and participatory observations on the background, role, identity, and labor experience of TV planning producers who appeared in the drama production process with the implementation of outsourcing production policy in 1991. The number of dramas produced increased sharply in the mid-2000s due to the Korean Wave. Against this backdrop, the planning producer has expanded their scope in the drama production process and emerged as a new drama producer. The planning producer plays a role in creating an environment in which writers and directors can be selected with the identity of "not a creator but a producer of dramas" and lead drama planning. OTT and watching TV on the Internet have made it possible to watch dramas without TV. As this phenomenon accelerates and becomes commonplace, fewer consumers adhere to the traditional way of watching dramas using TV, and consumers' emotional tastes become more demanding. In this environment, TV planning producers are leading the production of dramas, exerting as much influence as writers and directors. They are also building new power relationships among drama producers by securing planning and financial power.