• 한국환경과학회지
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• 제17권12호
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• pp.1325-1330
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• 2008

This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was $162{\mu}$mol/min/g protein. $I_{50}$ for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at $4^{\circ}C\;and\;25^{\circ}C$ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.

• Animal cells and systems
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• 제7권1호
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• pp.89-92
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• 2003

Immunoassays have become indispensable tools and achieved great importance in scientific and medical research. However, typical immunoassays are time-consuming and use complex, multi-step procedures. In this study, we introduce a new immunoassay system for the quantification of several analytes at a time without any washing steps. It is comprised of a detector solution with fluorescence-labeled antibodies and a test strip with immobilized capture antibodies. Using a micro-array scanner, the antigen-antibody complex was quantitatively determined by measuring the intensities of fluorescence on the capture lines or dots of nitrocellulose membrane. This method demonstrated its rapid quantitative determination of analytes without many processing steps as well as specific identification of multiple analytes in biological specimens.

• Bulletin of the Korean Chemical Society
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• 제17권4호
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• pp.338-342
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• 1996

A sensitive method for the determination of trace cadmium after the preconcentration by extracting its 8-hydroxyquinoline complex into a molten benzophenone was developed. Several experimental conditions such as the pH of solution, the amounts of 8-hydroxyquinoline and benzophenone, stirring time, and standing time were optimized. Trace cadmium in 100 mL water sample was chelated with 2.5 mL of 0.001 M 8-hydroxyquinoline at pH 8.0. After 0.07 g benzophenone was added, the solution was heated to about 70 ℃ and stirred vigorously for 1 minute to dissolve the complex quantitatively in a molten benzophenone, and stood for 30 minutes to reproduce the microcrystalline benzophenone. The benzophenone containing Cd-8-hydroxyquinoline complex was filtered and dissolved in acetone. Cadmium was determined by a flame atomic absorption spectrophotometry. The interfering effects of diverse concomitant ions were investigated and eliminated. This method could be applied to natural water samples and the recovery of more than 90% was obtained in the real samples.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.404.1-404.1
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• 2002

A LC/MS/MS method for the simultaneous determination of the activities of seven major human drug-metabolizing cytochrome P450s (CYP3A4. CYP2D6. CYP2C9. CYP1A2, CYP2C19, CYP2A6. and CYP2C8) was developed. This method used an in vitro cocktail of specific substrates (midazolam. bufuralol. diclofenac, ethoxyresorufin. S-mephenYlOin. coumarin. and paclitaxel) and LC/MS/MS. The assay incubation time is 20 min and the analysis time is 8 min/sample. (omitted)

• Journal of Positioning, Navigation, and Timing
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• 제13권2호
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• pp.149-158
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• 2024

Global Navigation Satellite System (GNSS) receivers are becoming increasingly sophisticated, equipped with advanced features and precise specifications, thus demanding efficient and high-performance hardware platforms. This paper presents the design and implementation of a Field-Programmable Gate Array (FPGA)-based GNSS receiver development platform for multi-band signal processing. This platform utilizes a FPGA to provide a flexible and re-configurable hardware environment, enabling real-time signal processing, position determination, and handling of large-scale data. Integrated signal processing of L/S bands enhances the performance and functionality of GNSS receivers. Key components such as the RF frontend, signal processing modules, and power management are designed to ensure optimal signal reception and processing, supporting multiple GNSS. The developed hardware platform enables real-time signal processing and position determination, supporting multiple GNSS systems, thereby contributing to the advancement of GNSS development and research.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1438-1442
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• 2003

A method for the determination of nitrogen in ruminant feedstuffs, products and excreta (e.g. milk and urine) using a spectrophotometer is developed, where samples processed for P determination are also used to determine N. Samples are digested with sulphuric acid and subsequently with hydrogen peroxide in Kjeldahl tubes. Digested solutions along with phenol and buffered alkaline hypochlorite reagents are incubated in a water bath at $37^{\circ}C$ for 30 min and presented in the spectrophotometer. The spectrophotometer set at 625 nm measures the concentration of N of each sample. Nitrogen in 261 of the samples was also determined by the classical Kjeldahl method in order to develop a relationship between N determined by the Kjeldahl method (Y) and the colorimetric method (X). The mean value of Y was as high as that of X (0.92 vs. 0.96; p>0.05). The colorimetric method predicted Kjeldahl N highly significantly (Y=0.985X-0.024, $R^2=0.993$, p<0.001; or more simply Y=0.974X, $R^2=0.993$, p<0.001). An analysis of regression found no difference (p>0.05; both t-test and F-test) between colorimetric (0.96% N) and adjusted (0.96% N) N. In comparison with the Kjeldahl method, the analytical capacity of N by colorimetric method increases greatly, where 200-300 determinations of N are possible in a working day. In addition, the system provides an opportunity to use not only the same digested solution for both N and P determination of a particular sample, but also uses the same spectrophotometer to assay both N and P. Therefore, the system may be attractive in situations where both elements of a sample are to be determined. In conclusion, the speed of N determination, low cost, efficient use of labour, time and reagents, fewer items of equipment, and the reduction of environmental pollution by reducing effluent and toxic elements are the advantages of this method of N determination.

• 한국콘텐츠학회논문지
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• 제17권12호
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• pp.516-524
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• 2017

본 연구에서는 최근 국내에서 확산되고 있는 장애아동을 위한 자기결정 집단프로그램의 효과크기를 통합하여 제시하고, 보다 효과적인 자기결정 집단프로그램의 실천전략을 제시하기 위하여 메타분석을 실시하였다. 자료 수집은 2017년 6월까지 국내에서 발표된 석 박사 학위논문 및 학술지에 게재된 논문 중 메타분석의 대상선정기준에 따라 최종 17편의 논문을 선정하여 분석하였다. 메타분석을 위해서는 CMA(Comprehensive Meta-Analysis) software 2.0을 사용하였다. 연구의 주요 결과는 다음과 같다. 첫째, 연구문제 1은 '자기결정 집단프로그램의 평균효과크기는 어느 정도인가'이었다. 연구결과, 랜덤효과모형에 따라 자기결정력의 효과크기는 1.695, 사회기술은 1.316로 나타나서 모두 큰 효과크기를 나타내고 있었다. 둘째, 연구문제 2는 '자기결정 집단프로그램은 조절변수에 따라 효과크기가 다른가?' 이었다 통계적으로 유의미한 변수는 '자기결정력'에 있어서 연령, 장애유형, 총 횟수, 주당 횟수, 1회당 시간, 진행자 전공 등이었다. 이러한 결과를 토대로 본 연구의 결과와 함의를 제언하였다.

• 대한공간정보학회지
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• 제23권4호
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• pp.67-74
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• 2015

GNSS/Leveling기술은 GNSS기술과 Leveling기술을 이용하여 기하학적 방법으로 지오이드고를 얻을 수 있게 해주며, GNSS/Geoid기술은 GNSS기술을 통해 얻은 타원체고에서 Geoid기술을 통해 얻은 지오이드고를 제하여 정표고를 얻는 기술을 말한다. 본 연구에서는 GNSS/Geoid기술을 이용한 표고결정 정확도를 검증하기 위하여 GNSS 타원체고 측정의 정확도를 검증하고자 하였다. 연구를 위하여 경남 지역에 테스트 베드(test bed)를 선정하고 GNSS 정적측위관측을 실시하였으며, 여러 가지 해석 조건에 따라 데이터를 처리함으로써 관측조건에 따른 GNSS 타원체고 측정의 정확도를 규명하였다. 연구결과 GNSS 정적측위방법에 의한 타원체고 결정에 있어서 3cm의 목표정확도를 확보하기 위해서는 측량지역 주변부의 네 점의 기지점을 고정하여 두 시간 이상 관측하여야 하며 기선거리는 20km로 제한하여야 한다는 것을 알 수 있었다.

• 한국동물생명공학회지
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• 제34권2호
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• pp.111-116
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• 2019

This study was conducted to analyze the specific genes associated with sex-determination in Korean native cow. The highly organized spermatogenesis requires accurate spatial and temporal regulation of gene expression, which is governed by transcriptional, post-transcriptional, and epigenetic processes. Recently, farmers have been interested in determining the sexual identity of the calves in their farm. We analyzed the sperm of Korean native and Holstein cows, which were supplied from Hanwoo Improvement Center. We evaluated sperm motility and expression of sperm-specific genes after treating semen with both male- and female reagents. Sperm motility in Korean native cows decreased by approximately 10% in the first 30 minutes after treatment with sex-determination reagent. However, sperm motility of Holstein cows decreased to 60-70% after 15 minutes and to 20-30% after 30 minutes. We selected six specific genes expressing in the spermatozoa to analysis the gene expression level. The Real-time PCR results suggest that the selected genes (Gimap4, Tmeff1, Rac2, Abi2, Rac1, and Clu) were highly expressed in the group treated with the male reagent compared to the group treated the female reagent and to the untreated-group (control). In the present study, we suggest that the selected genes play a pivotal role in sex-determination.

• Nuclear Engineering and Technology
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• 제48권3호
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• pp.727-732
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• 2016

A simple and rapid spectrophotometric method has been developed to enable the determination of plutonium concentration in an irradiated fuel solution in the presence of all fission products. An excess of ceric ammonium nitrate solution was employed to oxidize all the valence states of plutonium to +6 oxidation state. Interference due to the presence of fission products such as ruthenium and zirconium, and corrosion products such as iron in the envisaged concentration range, as in the irradiated fuel solution, was studied in the determination of plutonium concentration by the direct spectrophotometric method. The stability of plutonium in +6 oxidation state was monitored under experimental conditions as a function of time. Results obtained are reproducible, and this method is applicable to radioactive samples resulting before the solvent extraction process during the reprocessing of fast reactor spent fuel. An analysis of the concentration of plutonium shows a relative standard deviation of <1.2% in standard as well as in simulated conditions. This reflects the fast reactor fuel composition with respect to uranium, plutonium, fission products such as ruthenium and zirconium, and corrosion products such as iron.