• Nutrition Research and Practice
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• v.17 no.4
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• pp.682-697
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• 2023

BACKGROUND/OBJECTIVES: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice. MATERIALS/METHODS: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddH2O (H group) for 15 days. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mice was detected. Transcriptome sequencing technology was used to investigate the molecular mechanisms underlying the antioxidant and lipid-lowering effects of Tibetan tea in mice. Furthermore, the expression levels of liver antioxidant and lipid metabolism related genes in various groups were detected by the real-time quantitative polymerase chain reaction (qPCR) method. RESULTS: The results showed that a total of 42 flavonoids are provisionally annotated in Tibetan tea using KGMN strategies. Tibetan tea significantly reduced body weight gain and increased T-AOC and SOD activities in mice compared with the H group. Based on the results of transcriptome and qPCR, it was confirmed that Tibetan tea could play a key role in antioxidant and lipid lowering by regulating oxidative stress and lipid metabolism related pathways such as insulin resistance, P53 signaling pathway, insulin signaling pathway, fatty acid elongation and fatty acid metabolism. CONCLUSIONS: This study was the first to use computational tools to deeply explore the composition of Tibetan tea and revealed its potential antioxidant and hypolipidemic mechanisms, and it provides new insights into the composition and bioactivity of Tibetan tea.

• Journal of Dairy Science and Biotechnology
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• v.39 no.2
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• pp.68-77
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• 2021

This study aimed to evaluate the effects of antibacterial activity of Tibetan mushroom exopolysaccharide against foodborne pathogenic bacteria Staphylococcus aureus 305, Listeria monocytogenes ATCC19114, Escherichia coli O157:H7 ATCC42894 and Escherichia coli O55. The yield of exopolysaccharide isolated from Tibetan mushroom culture was 620 mg/L. The antibacterial activity of exopolysaccharide against foodborne pathogenic bacteria exhibited 15 mm and 12 mm clear zone against S. aureus 305 and L. monocytogenes ATCC 19114, respectively. However, no clear zone was observed against E. coli O157:H7 ATCC 42894 and E. coli O55. In conclusion, exopolysaccharide isolated from Tibetan mushroom culture have the antibacterial activity only against Gram-positive foodborne pathogenic bacteria.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1102-1106
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• 2009

The Tibetan Mastiff is one of the most archaic, ferocious and the largest dogs in the world. The Kunming dog is the chief working-dog breed in China. In this study, ten microsatellite loci were used to assess the genetic diversity and evolutionary relationships in six dog breeds, including Tibetan Mastiff, Kunming dog, Belgian Malinois, Labrador Retriever, English Springer Spaniel, and German Shepherd. The highest genetic diversity was exhibited by the Tibetan Mastiff, indicating useful protection and little inbreeding in the modern Tibetan Mastiff. Higher genetic diversity was observed in European breeds, supporting the hypotheses that breeders outcross their pure breed dogs occasionally to avoid deleterious effects in Europe. Evolutionary relationships showed that English Springer Spaniel and Labrador Retriever were clustered together, then with the Tibetan Mastiff, consistent with previous cluster results. German Shepherd and Kunming dog were grouped together, coinciding with the breeding history of Kunming dog. It is the first time that Tibetan Mastiff and Kunming dog have been analyzed with microsatellites.

• Journal of the Daesoon Academy of Sciences
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• v.19
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• pp.81-98
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• 2005

Nepal's role in the expansion of Mahayana Buddhism beyond the Himalaya is very significant. Nepal became the center of Mahayana Buddhism after the Muslim invasion of Nalanda Mahavihara in the 1199 A.D., which she maintained almost for 300 years. During this period, Nepal had produced a large number of profound Buddhist scholars. Most of them were the teachers of eminent Tibetan Buddhists. Some of the Nepalese Gurus also has continued lineage in Tibet until now. During that time, every Tibetan had desire to go to Nepal for higher Buddhist learning. As a matter of fact, many Tibetans made arduous journey across the Himalaya to fulfill the dream. Tibetan studied various forms of tantra, precepts, logic, doctrine, Sutra, Sadhana, Doha, Charyagiti, meditation etc. under direct supervision of Nepalese teachers. Great Tibetan scholars such as Marpa, Rwa Lo, Chag Lo, Khon phu ba, Klog Lo, Gos Lo, and others were the product of Nepal's scholarly tradition. They have significant place in the history of Tibet. Nepalese scholars also frequently visited Tibet where they taught Buddhism in various monasteries. They also had major role in propagating tantra in Tibet. Tibetans firmly believe that it is not possible to attain enlightenment without practicing tantra. The contribution of Nepalese scholars was so profound that Tibet produced many eminent scholars who developed scholastic tradition in Tibet. But after 14th century, Nepal's scholarly tradition ceased to continue. Then after, Tibetans started to call them 'the dull'.

• Animal Bioscience
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• v.34 no.5
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• pp.922-930
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• 2021

Objective: Tibetan pigs, predominantly originating from the Tibetan Plateau, have been subjected to long-term natural selection in an extreme environment. To characterize the metabolic adaptations to hypoxic conditions, transcriptomic and proteomic expression patterns in the livers of Tibetan and Yorkshire pigs were compared. Methods: RNA and protein were extracted from liver tissue of Tibetan and Yorkshire pigs (n = 3, each). Differentially expressed genes and proteins were subjected to gene ontology and Kyoto encyclopedia of genes and genomes functional enrichment analyses. Results: In the RNA-Seq and isobaric tags for relative and absolute quantitation analyses, a total of 18,791 genes and 3,390 proteins were detected and compared. Of these, 273 and 257 differentially expressed genes and proteins were identified. Evidence from functional enrichment analysis showed that many genes were involved in metabolic processes. The combined transcriptomic and proteomic analyses revealed that small molecular biosynthesis, metabolic processes, and organic hydroxyl compound metabolic processes were the major processes operating differently in the two breeds. The important genes include retinol dehydrogenase 16, adenine phosphoribosyltransferase, prenylcysteine oxidase 1, sorbin and SH3 domain containing 2, ENSSSCG00000036224, perilipin 2, ladinin 1, kynurenine aminotransferase 1, and dimethylarginine dimethylaminohydrolase 1. Conclusion: The findings of this study provide novel insight into the high-altitude metabolic adaptation of Tibetan pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.61-67
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• 2010

The chemical components in plant cuticular wax can be used as markers to estimate the species composition of the diet of grazing animals. In this experiment, composition of the diet of yak on the Qinghai-Tibetan plateau was estimated using n-alkane analysis. During the grazing period, samples of whole plants of the species present, plus fecal samples voided by the yak, were collected, air-dried and ground prior to the extraction of cuticular wax n-alkanes. The species composition of the yak diets was estimated by relating fecal alkane contents to those of the plant species, using the 'ATWHAT'software package. The results showed that the n-alkane technique can detect the main dietary components selected by yak. The diet consumed by yak contained 33% Kobresia humilis, 67% Stipa aliena in summer pasture; 26% Potentilla anserine, 74% Carex qinghaiensis in autumn pasture; 52% Carex qinghaiensis, 32% Heteropappus bowerii and 16% Saussurea semifasciata in winter pasture and 5% Carex qinghaiensis, 95% Achnatherum splendens in spring pasture. The apparent selection for forbs is likely to be a reason for nutritional constraint of yak inhabiting alpine environments.

• Journal of the Korean Society of Costume
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• v.64 no.4
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• pp.131-149
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• 2014

Tibetans are one of the ethnic Chinese minorities, and this research examined the characteristics and features of their traditional costumes by investigating their historical, geographical and cultural background. This research also examined the cultural elements according to their characteristics based on Tibet's nature, religion, arts culture such as the Jang-po, Chin-sam, Kam-kjun and belts, which are used to fix the costumes. Based on such studies the traditional Tibetan costumes were set as a motif and were expressed appropriately into a contemporary style. By studying the composition of its costumes and its costume culture, the characteristics of the Tibetan costumes could be classified into four features. First, the 'variability of form' expresses a new type depending on how it is worn. Second, the 'color contrast' shows a strong visual contrast. Third, the 'difference of materials' combines different materials into one. Fourth, the 'symbolic meaning' grants meaning to the wearer. As a result of this study, by using the characteristics of the Tibetan nature culture, religion culture and art culture as a basis, two suits of clothes for each characteristics and six suits of clothes were produced as a fashion design product.

• Journal of Sasang Constitutional Medicine
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• v.19 no.3
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• pp.300-307
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• 2007

We have studied the history of the introduction of Ayurveda medicine in Mongolia. During the periods of the Hunnu (400 BC-200 AD), Ikh Nirun (400-600 AD), and Uigur Dynasty (800-1,000 AD), Ayurveda (Indian Medicine) was introduced to Mongolia along with Buddhism from the Middle Asian countries Kushan, Khotan, Sogd and Uigar. Ayurveda was fully introduced to Mongolia under the deep influence of Tibetan Buddhism from the 13th century. Mongolia's first Medical School, following the Tibetan tradition, was established in 1662. In Mongolia more than 40 Medical Schools were established from 1662-1937. 26 Ayurvedic treatises were translated into the Mongolian language and published in 1742-1749. Since the $14^{th}$ century Mongols have been translating Tibetan Medical books into the Mongolian language, of which we have today found more than ten. Over the centuries, Mongolian scholars have written many commentaries to these medical texts.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.9
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• pp.1203-1208
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• 2001

Linzhi Native Pig is a unique local breed recently discovered in the hinterland of Tibet. Its geological distribution, natural environment and ecological conditions have been explored. Using random sampling in typical colony of classification and standard animal-scientific and biogenetic techniques, we examined its contour features, size and weight, reproductive performances, carcass characters, meat quality, fresh-keeping features and the frequency distribution in the 19 structural gene loci encoding enzymes and proteins; according to folklores and Tibetan, Chinese and English history books, the materials and literature of Tibetan Studies, we have analyzed its origin and affirmed the fact that its products have been consumed as Tibetan medicine resources. Our findings make certain that Linzhi Native Pig holds great potential value in economy and culture.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.313-315
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• 2007

Seventeen haplotypes were detected from the complete mitochondrial DNA control region sequences analyzed from eighty individuals of two Tibetan domestic sheep breeds. The nucleotide composition of all the sequences was 33.0% A, 29.7%T, 22.9%C and 14.4%G; G+C was 37.3%. The length of the sequences ranged from 1,107 bp to 1,259 bp. The difference between them was primarily due to 3-5 copy numbers of a 75 bp tandem repeat sequence. The NJ phylogenetic tree (the number of replications of bootstrap test is 1,000) presented three major domestic sheep lineages, which suggested that modern Tibetan sheep breeds are derived from three maternal sources.