• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.963-968
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• 2013

Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.379-385
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• 2004

Two trials in vivo and in vitro were conducted, in vivo to determine the apparent digestibility of gross energy, crude protein, dry matter, acid detergent fiber, neutral detergent fiber and apparent digestible energy in 10 corn by-products. In vivo the diets included one basal corn diet, four corn gluten meal diets, four corn distillers dried grains with solubles diets and two corn distillers dried grains diets using the different methods, 12 crossbred barrows weigh $40{\pm}$1.6 kg were allocated into individual metabolic crate, according to a $6{\times}6$ Latin square design. In vitro using flask technique, filter bag technique and dialysis tubing technique, the digestibilities of gross energy, crude protein and dry matter in corn gluten meal and corn distillers dried grains with solubles were investigated. Pepsin, pancreatin, intestinal fluid, rumen fluid and cellulase were used in incubation. The results showed that correlation coefficient was 0.73 in corn distillers dried grains with solubles between the digestibility of crude protein and acid detergent fiber in vivo (p<0.01); and correlation coefficient was 0.68 in corn distillers dried grains with solubles between the digestibility of gross energy and neutral detergent fiber in vivo (p<0.01). Apparent digestible energy (DE) of corn by-products in pig total tract was predicted by the percentage of crude protein (CP) and the content of gross energy (GE) in feedstuff. The equation: DE=5,601.09+26.69$\times$CP %-0.5904$\times$GE, ($R^2=0.72$). In vitro, filter bag technique was more convenient; furthermore, the digestibility for the treatments (pepsin+pancreatin+rumen fluid and pepsin+pancreatin+cellulase) was better.

• Biomolecules & Therapeutics
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• 제25권6호
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• pp.599-608
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• 2017

Tanshinone IIA (Tan IIA) is a pharmacologically active substance extracted from the rhizome of Salvia miltiorrhiza Bunge (also known as the Chinese herb Danshen), and is widely used to treat atherosclerosis. The pregnane X receptor (PXR) is a nuclear receptor that is a key regulator of xenobiotic and endobiotic detoxification. Tan IIA is an efficacious PXR agonist that has a potential protective effect on endothelial injuries induced by xenobiotics and endobiotics via PXR activation. Previously numerous studies have demonstrated the possible effects of Tan IIA on human umbilical vein endothelial cells, but the further mechanism for its exerts the protective effect is not well established. To study the protective effects of Tan IIA against hydrogen peroxide ($H_2O_2$) in human umbilical vein endothelial cells (HUVECs), we pretreated cells with or without different concentrations of Tan IIA for 24 h, then exposed the cells to $400{\mu}M$ $H_2O_2$ for another 3 h. Therefore, our data strongly suggests that Tan IIA may lead to increased regeneration of glutathione (GSH) from the glutathione disulfide (GSSG) produced during the GSH peroxidase-catalyzed decomposition of $H_2O_2$ in HUVECs, and the PXR plays a significant role in this process. Tan IIA may also exert protective effects against $H_2O_2$-induced apoptosis through the mitochondrial apoptosis pathway associated with the participation of PXR. Tan IIA protected HUVECs from inflammatory mediators triggered by $H_2O_2$ via PXR activation. In conclusion, Tan IIA protected HUVECs against $H_2O_2$-induced cell injury through PXR-dependent mechanisms.

• 한국항만경제학회지
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• 제26권1호
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• pp.259-276
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• 2010

본 연구에서는 국제적인 공항과 항만을 동시에 지닌 인천의 복합운송시스템 구축을 위한 역할과 과제는 무엇인지 살펴보기 위해 물류기업 및 공공기관, 연구기관 임직원을 대상으로 개별 심층면접조사(Depth Interview)를 실시하여 국제복합운송 물류거점 선점을 위한 경쟁력 요인선정, 주요 도시별 복합운송측면에서의 경쟁력 점수를 측정하였다. 주요 도시별 경쟁력 최종 점수를 100점 만점으로 환산하여 살펴보면 1위는 상해(64.8점), 2위 홍콩(64.5점), 3위 인천(62.9점), 4위 부산(60.4점) 등으로 나타나 인천이 향후 동북아지역에서 국제복합운송 물류거점으로서 발전할 수 있는 잠재력은 어느 정도 보유한 것으로 평가되었다. 또한 본 연구 분석 결과를 토대로 인천지역이 동북아 지역의 복합운송거점으로서의 나아가야 할 발전방안에 대해서 제시하면 첫째, 인천-천진간 해공복합운송 공동물류센터 건립 및 제도 간소화 협정체결사업이 필요하며, 둘째, 인천-상해간 해륙복합운송활성화를 위한 페리-트럭 복합운송 활성화를 위한 선박에서 통관절차 수행 등의 제도 개선 추진사업이 요구된다.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.898-904
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• 2014

The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and $95^{\circ}C$. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The $k_{cat}/K_m$ value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased $K_m$, and consequently increased the specific activity.

• 디지털융복합연구
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• 제18권1호
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• pp.111-119
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• 2020

본 연구에서는 한국을 방문한 개별 여행자들의 디지털 기록을 수집하여 정량적 통계 분석과 소셜 네트워크 분석(SNA)을 통해 한국을 방문하는 개별 중국 여행자들의 한국 관광 경로의 특징을 분석하였다. 연구결과 서울, 제주도, 부산 및 대구는 한국을 찾는 중국 여행자들의 주요 방문 장소이며, 중국의 청도, 천진, 심양, 홍콩, 포산 및 마카오는 한국을 찾는 중국 개별 여행자들의 주요 중계지 임을 알 수 있었다. 본 연구는 한국을 찾는 중국 개별 여행자들의 표본 특성과 각 여행 노드의 기능적 위치설정을 정확히 확인함으로써 정밀한 관광 마케팅과 관광 노선 개발에 활용할 수 있는데 의의가 있다. 향후 본 연구에 사용된 데이터의 추출 기간을 확대하고 더 많은 샘플을 확보하여 관광 경로의 연간 변동특성과 주요 방문지의 변동 특성을 분석할 필요가 있다.

• Membrane and Water Treatment
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• 제6권3호
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• pp.205-224
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• 2015

A novel hydrophilic poly (vinylidene fluoride)/poly (p-phenylene terephthalamide) (PVDF/PPTA) blend membrane was prepared by in situ polycondensation of p-phenylene diamine (PPD) and terephthaloyl chloride (TPC) in PVDF solution with subsequent nonsolvent induced phase separation (NIPS) process. For comparison, conventional solution blend membrane was prepared directly by adding PVDF powder into PPTA polycondensation solution. Blend membranes were characterized by means of viscometry, X-ray photoelectron spectroscopy (XPS), Field Emission Scanning Electron Microscopy (FESEM). The effects of different blending methods on membrane performance including water contact angle (WCA), mechanical strength, anti-fouling and anti-compression properties were investigated and compared. Stronger interactions between PVDF and PPTA in in situ blend membranes were verified by viscosity and XPS analysis. The incorporation of PPTA accelerated the demixing rate and caused the formation of a more porous structure in blend membranes. In situ blend membranes exhibited better hydrophilicity and higher tensile strength. The optimal values of WCA and tensile strength were $65^{\circ}$ and 34.1 MPa, which were reduced by 26.1% and increased by 26.3% compared with pure PVDF membrane. Additionally, antifouling properties of in situ blend membranes were greatly improved than pure PVDF membrane with an increasing of flux recovery ratio by 25%. Excellent anti-compression properties were obtained in in situ blend membranes with a stable pore morphology. The correlations among membrane formation mechanism, structure and performance were also discussed.

• 한국항만경제학회지
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• 제27권4호
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• pp.271-289
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• 2011

중국과 러시아에 둘러쌓인 내륙국 몽골은 그동안 유럽과 아시아 국가들과 교역을 다양화하려 추진하여 왔다. 2009년 이후의 지속적 광물자원 가격의 상승은 몽골이 교역을 다양화하고 확대하는 데 도움이 되어 왔다. 그럼에도 불구하고, 중국과 러시아를 통과하는 데 국경에서 규제와 장애는 다양하며 복잡하다. 또한 몽골에서 중국과 러시아 항만까지 거리는 각기 1700km와 3800km로 몽골 수출입화물 운송에 고물류비 부담을 초래하고 있다. 이 연구는 몽골 광물자원의 효율적 물류체계에 대한 통과운송의 문제와 장애들을 논의한 후 통과운송을 개선하는 데 필요한 국제복합운송에 대한 제안과 국제협력 방안을 제시한다. 몽골은 천진항 경유 경로 외에 다른 국제복합운송 경로를 모색해야 하며. 장비의 확보를 위한 국제협의체구성, 국경통과시 통과절차의 개선, 새로운 국제복합운송 경로의 재무적 타당성 제고를 추진해야 한다.

• The Korean Journal of Pain
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• 제33권3호
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• pp.216-225
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• 2020

Background: Garlic oil is a rich source of organosulfur compounds including diallyl disulfide and diallyl trisulfide. There have been studies showing the neuroprotective actions of these organosulfur compounds. However, the potential of these organosulfur compounds in neuropathic pain has not been explored. The present study was aimed at investigating the pain attenuating potential of diallyl disulfide and diallyl trisulfide in chronic constriction injury (CCI)-induced neuropathic pain in rats. The study also explored their pain-attenuating mechanisms through modulation of H₂S, brain-derived neurotrophin factor (BDNF) and nuclear factor erythroid 2-related factor 2 (Nrf2). Methods: The rats were subjected to CCI injury by ligating the sciatic nerve in four places. The development of neuropathic pain was measured by assessing mechanical hyperalgesia (Randall-Selittotest), mechanical allodynia (Von Frey test), and cold allodynia (acetone drop test) on 14th day after surgery. Results: Administration of diallyl disulfide (25 and 50 mg/kg) and diallyl trisulfide (20 and 40 mg/kg) for 14 days led to a significant reduction in pain in CCI-subjected rats. Moreover, treatment with these organosulfur compounds led to the restoration of H₂S, BDNF and Nrf2 levels in the sciatic nerve and dorsal root ganglia. Co-administration of ANA-12 (BDNF blocker) abolished pain attenuating actions as well as BDNF and the Nrf2 restorative actions of diallyl disulfide and diallyl trisulfide, without modulating H₂S levels. Conclusions: Diallyl disulfide and diallyl trisulfide have the potential to attenuate neuropathic pain in CCI-subjected rats possibly through activation of H₂S-BDNF-Nrf2 signaling pathway.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제16권4호
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• pp.327-344
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• 2009

In this paper, we deal with the following system of nonlinear singular boundary value problems(BVPs) on time scale $\mathbb{T}$ $$\{{{{{{x^{\bigtriangleup\bigtriangleup}(t)+f(t,\;y(t))=0,\;t{\in}(a,\;b)_{\mathbb{T}},}\atop{y^{\bigtriangleup\bigtriangleup}(t)+g(t,\;x(t))=0,\;t{\in}(a,\;b)_{\mathbb{T}},}}\atop{\alpha_1x(a)-\beta_1x^{\bigtriangleup}(a)=\gamma_1x(\sigma(b))+\delta_1x^{\bigtriangleup}(\sigma(b))=0,}}\atop{\alpha_2y(a)-\beta_2y^{\bigtriangleup}(a)=\gamma_2y(\sigma(b))+\delta_2y^{\bigtriangleup}(\sigma(b))=0,}}$$ where $\alpha_i$, $\beta_i$, $\gamma_i\;{\geq}\;0$ and $\rho_i=\alpha_i\gamma_i(\sigma(b)-a)+\alpha_i\delta_i+\gamma_i\beta_i$ > 0(i = 1, 2), f(t, y) may be singular at t = a, y = 0, and g(t, x) may be singular at t = a. The arguments are based upon a fixed-point theorem for mappings that are decreasing with respect to a cone. We also obtain the analogous existence results for the related nonlinear systems $x^{\bigtriangledown\bigtriangledown}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangledown\bigtriangledown}(t)$ + g(t, x(t)) = 0, $x^{\bigtriangleup\bigtriangledown}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangleup\bigtriangledown}(t)$ + g(t, x(t)) = 0, and $x^{\bigtriangledown\bigtriangleup}(t)$ + f(t, y(t)) = 0, $y^{\bigtriangledown\bigtriangleup}(t)$ + g(t, x(t)) = 0 satisfying similar boundary conditions.