• The Korea Journal of Herbology
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• v.34 no.4
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• pp.19-25
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• 2019

Objectives : Thymol (2-isopropyl-5-methylphenol), a natural monoterpenoid phenol, is one of the major odorant constituents found in natural essential oils of various herbal plants, such as Thymus quinquecostatus and Thymus vulgaris. Multiple biological activities of thymol, including antioxidative, antimicrobial, and anti-inflammatory effects, have been reported in numerous in vitro studies, and recently it was suggested that thymol may could inhibit oxidization of L-dihydroxyphenylalanine (L-DOPA) to dopaquinone required in melanogenesis pathway, as an antioxidant. Methods : MTT assay was performed to test the cytotoxic effect of thymol in B16F10 cells. Inhibitory effect of thymol to tyrosinase activities were examined using both mushroom tyrosinase and intracellular tyrosinase. Expression level of tyrosinase in B16F10 cells were investigated by western blot analysis. Results : The cell viability was decreased by thymol treatment in dose-dependant manner, leading significant cytotoxicity in 500 and $1000{\mu}M$ thymol-treated groups. In the alpha-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis, administration of thymol significantly decreased extracellular (secreted) melanin content in dose-dependent manner. Cellular tyrosinase activity assay and western blot analysis of intracellular tyrosinase showed that thymol has a strong anti-melanogenic effect by inhibition of tyrosinase activity and by decreasing expression of tyrosinase that contribute to melanin synthesis in the B1610 cells. Conclusions : As the first functional study that prove anti-melanogenic effect of thymol and its underlying mechanism in the living cells, our study suggests the applicability of fragrance as the functional materials of cosmetics or health supplement, not as just an additive.

• Food Science and Preservation
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• v.12 no.4
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• pp.344-349
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• 2005

Microwave-assisted process (MAP) was applied to extract antioxidant-related components from Thymus quinquecostatus var. japonica Hara. Microwave power(2,450 MHz, $0{\sim}160$ W) and extraction time($1{\sim}5\;min$) were used as independent variables($X_i$) for central composite design to yield 10 different extraction conditions. Response surface methodology was applied to predict optimum extraction conditions for dependent variables of extracts, such as total yield, total phenolics, flavonoid, and electron donation ability depending on different powers and extraction times of MAP. Determination coefficients($R^2$) of regression equations for dependent variables were higher than 0.93 excluding that of total phenolics, and microwave power was predicted more influential than extraction time in MAP (p<0.05). The optimal extraction time for each dependent variable was ranged from 3.36 to 4.97 min, but microwave power showed wide ranges depending on variables. The superimposed contour maps for maximized dependent variables illustrated extraction conditions of 64 to 100 W in microwave power and 2.9 to 4.0 min in extraction time.

• KIEAE Journal
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• v.13 no.5
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• pp.89-96
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• 2013

Green roofs can reduce surface water runoff, provide a habitat for wildlife moderate the urban heat island effect, improve building insulation and energy efficiency, improve the air quality, create aesthetic and amenity value, and preserve the roof's waterproofing. Green roofs are mainly divided into three types : intensive, simple-intensive, and extensive. Especially, extensive roof environment is a harsh one for plant growth; limited water availability, wide temperature fluctuations, high exposure to wind and solar radiation create highly stressed environment. This study, aimed at extensive green roof, was carried out on the rooftop of the library at Seoul Women's Univ. from October to November, 2012 and from March to August, 2013. To suggest the most effective vegetation model for biodiversity and heat island mitigation, surface temperatures were monitored by each vegetation model. We found that herbaceous plants of Aster sphathulifolius, Aceriphyllum rossii and Belamcanda chinensis, shrub of Syringa patula 'Miss Kim', Thymus quinquecostatus var. japonica, Sedum species can mixing each other. Among them, the vegetation models including Sedum takesimense, Aster sphathulifolius, Thymus quinquecostatus var. japonica was more effective on the surface temperature mitigation, because the species have the tolerance and high ratio of covering, and also in water. Especially, in the treatment of bark mulching, they helped to increase the temperature of vegetation models. In the case of summer, temperature mitigation of vegetation models were no significant difference among vegetation types. Compared to surface temperature of June, July and August were apparent impact of temperature mitigation, it shows that temperature mitigation are strongly influenced by substrate water content.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.84-84
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• 2022

백리향(Thymus quinquecostatus)은 꿀풀과의 낙엽반관목으로 국내에서 자생하는 허브 식물 중 하나이다. 백리향은 특유의 좋은 향기 및 항산화, 항염증, 항균, 미백 등의 효능을 가지는 각종 폴리페놀 성분을 함유하고 있어 의약품이나 기능성 식품, 화장품의 천연 소재 원료로 활용되고 있다. 국내에서 백리향은 재배 환경에 맞추어 주로 고산지대의 노지에서 재배되고 있다. 노지 재배는 지역, 시기, 기후 등의 외부환경에 영향을 받아서 백리향의 유효성분, 품질 및 생산성을 안정적으로 유지하기 어렵다는 한계를 가지고 있다. 따라서 본 연구에서는 스마트팜 시스템을 활용하여 백리향의 유효 성분 등을 안정적으로 얻기 위한 생장조건을 탐색하기 위해 4종의 Light Emitting Diode(LED) 광원과 4종의 토양 조성에 따라서 백리향의 생육조건을 수행하였다. LED는 white, purple, RGB1, RGB2를 사용하였으며, 토양은 상토:펄라이트 비율(상토, 5:1, 3:1, 1:1)로 조성하여 백리향 묘목을 이식한 뒤 생장과 개화시기를 분석하였다. 재배환경은 백리향 재배지의 기상 데이터를 참고하여 동일하게 설정하였으며, 총 8주 동안 생육상태를 관찰하였다. 연구 결과 백리향 재배 4주차에 일부 개체에서 봉오리가 올라오며 개화를 시작하였으며, 8주차에는 대부분의 조건에서 개화를 관찰할 수 있었다. 백리향의 지상부 면적을 비교한 결과 가장 우수한 생장을 보이는 조건은 토양은 3(상토):1(펄라이트) 비율로 분석되었다. 따라서 이번 연구 결과를 바탕으로 백리향 재배에 스마트팜 농업 기술을 활용한다면 기존 노지 재배 한계를 보완하여 안정적이고 지속적인 백리향을 생산할 수 있을것으로 기대된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.81-81
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• 2020

Pneumonia is infection of air sacs in lungs by bacteria, viruses, fungi and abnormal atmosphere condition. That causes to inflammation and fills up with pus and fulid in air sacs. The symptoms of pneumonia are heavy cough, fever, shortness and rapid respiration and sputum with blood. Also, that remains sequela after the recovery. Thymus quinquecostatus (TQ) has a strong antibacterial effect. That has been used a natural medicine for bronchitis, asthma and nervous inflammation. In this study evaluated anti-inflammatory effects of TQ on IFN (interferon)-γ/TNF (tumor necrosis factor)-α (20 ng/ml) stimulation. MRC-5 cells were seeded at 1 × 10⁵ cells/well in a 24-well plate and stabilized overnight at 37℃. The cells were treated with various concentrations of TQ extracts (DW, 30, 50, 70, 95% EtOH, 0.1~100 mg/ml) for 4h, Subsequently IFN-γ/TNF-α (20 ng/ml) was added to each well and incubated over 12h. the production of inflammatory cytokines were measured by Enzyme-linked immunosorbentassay(ELISA) method. The absorbance was measured at 450 nm using microplate reader. The treatment with TQ extract at dose of 0.1 to 100 mg/ml did not show cytotoxicity in MRC-5 cells (showed cytotoxicity at 95% EtOH 100 mg/ml). The results demonstrated that stimulation of TNF-α/IFN-γ significantly increased the production of inflammatory mediators in the MRC-5 cells compared with unstimulated cells. By contrast, treatment with TQ extreats doses of 0.001 to 10 mM significantly suppressed the production of IL-6 and IL-8. Especially. The higher percent of ethyl alcohol extracts suppressed the production of inflammatory cytokines. This results are considered that essential oil of TQ is extracted according to ethyl alcohol percent. Therefore, TQ has potential to mitigate pulmonary disease that can be used a medicine for pneumonia

• Journal of Bio-Environment Control
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• v.20 no.1
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• pp.14-20
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• 2011

Objective of this research was to investigate the volatile aroma compounds and phenol contents for preservative effects. Aerial part of 5 Korean natured species of thyme located in Jeju alpine, jeju middle mountain, Kyeonggido, Ulleung island, and Gangwondo was analyzed by thermal desorption gas chromatograph mass spectrometer (TD-GC-MSD) method. Jeju middle mountain thyme was relatively high 62 species and has been investigated a high concentration of $7365.22{\mu}g/m^3$ contents according to the quantitative analysis. Total phenol contents containing thymol and carvacrol of Jeju middle mountain thyme were relatively high and showed 35.92%.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.239-244
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• 2009

In the current study, inhibitory activity of 8 selected Korean edible plants of Lamiaceae family against $\alpha$-glucosidases, prepared from rat small intestine acetone powder was evaluated. Total flavonoids and oxygen radical absorbance capacity (ORAC) were also investigated. Methyl alcohol extracts of Scutellaria indica (SI) had the highest $\alpha$-glucosidase inhibitory activity relevant for potentially managing hyperglycemia, followed by Clinopodium gracile (CG) and Thymus quinquecostatus (TQ). These 3 species also showed significant antioxidant activity in ORAC system. The $\alpha$-glucosidase inhibitory activity of the extracts was compared to selected phenolics. Among the standard phenolics tested quercetin which was major flavonoid in the extracts had the highest $\alpha$-glucosidase inhibitory activity. CG, TQ, and SI which had high quercetin content and ORAC values also exhibited significant sucrase inhibitory activity. Results suggested that selected 3 Korean Lamiaceae species have the potential development of effective dietary strategy for postprandial hyperglycemia and oxidative stress-linked diabetes complications.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.55-69
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• 2016

Significant efforts are being devoted in mitigating the urban heat island effect, and extensive green roofs are an option for mitigation. The purpose of this study was to compare the surface temperature, vegetation types, and plant species on an extensive green roof. Test beds were created in May 2015, and the surface temperature was monitored from June to August. The test beds comprised polyculture and monoculture. Polyculture was divided into three types, and monoculture comprised eight plant species. An extensive green roof is effective in reducing temperature by forming a shade and preventing sunlight from falling on the surface of buildings, which mitigates the urban heat island effect. Consequently, the surface temperature of the green roof and that of concrete during summer reduced from $17.8^{\circ}C$ to $7.3^{\circ}C$. The temperature reduction was greater on using polyculture than on using monoculture, but monocultures of Sedum takesimense, Hemerocallis dumortieri, Allium senescens, Aster yomena, Belamcanda chinensis, and Aster koraiensis also produced good results. The temperature reduction effects of Polygonatum odoratum var. pluriflorum f. variegatum, Phlox subulata, and Thymus quinquecostatus var. japonica were excellent compared with those of concrete but were less than those of other plant species. Careful attention is needed for the management of extensive green roofs. Studies on the plant species and types of extensive green roof should continue to mitigate the urban heat island effect.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.468-474
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• 2009

Thyme leaves of aerial part in 5 domestic species was extracted by several solvens, and chemical composition of extracts was analyzed by GC-MS to determine the effective solvent for phenol compound extracts. Total phenol contents containing thymol and carvacrol according to methanol extracts of Ulleung island, Jeju middle mountain, Kyeonggido, Jeju alpine species, and Gangwondo were 92.21, 84.92, 77.43, 74.16, and 73.51%, while them of ethanol extracts of Gangwondo, Jeju middle mountain, Kyeonggido, Ulleung island, and Jeju alpine species were in the order of and their contents were 93.64, 93.62, 90.74, 89.52, and 72.65%, separately. Hexane extracts of Gangwondo and Ulleung island thyme were almost composed by phenol contents containing thymol for 100 and 95.71%.

• Journal of Environmental Science International
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• v.12 no.2
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• pp.125-132
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• 2003

This study was executed by China along with the Tumen river, which formed border of North Korea and China. As a result 91 family, 282 genera, 363 species, 57 variety 4 species, total 424 taxa of vascular plants were identified. The examination area was include the region that had various vegetation such as upper swampy land, seashore dune, a river, a high mountain grassy plain, deciduous tree zone, evergreen coniferous forest zone. The followings are representative plants, which found in an examination area but not in South Korea. Picea koraiensis, Larix gmelini var. principis-ruprechtii, Betula playphylla, Ulmus pumila, Populus koreana, Ledum palustre var. diversipilosum, Rhododendron parvifolium, Salix brachypoda, Phacelurus latifolius, Carex gotoi, Carex capricornis, Calla palustris, Aquilegia flabellata var. pumila, Lilium davuricum, Astragalus membranaceus, Trifolium lupinaster, Ledebourieila seselodeds, Polemonium racemosum, Scutellaria baicalensis, Linnaea borealis, Thladiantha dubia, Inula helenium, Trollius hondoensis, Hypocaeris ciliata, Aconitum kirinense, Aconitum arcuatum, Potentilla viscosa var. macrophylla. And there are 21 rare and endangered species, which Korean Forest Sevice designated, such as Aconitum koreanum, Acorus calamus var. angustatus, Polygonatum stenophyllum, Drosera rotundifolia, Iris setosa, Jeffersonia dubia, Lilium davuricum, Lilium cernum, Paeonia obovata, Penthorum chinense, Phacelurus latifolius, Rosa marretii, Sparganium stoloniferum, Thymus quinquecostatus, Trillium tschonoskii, Utricularia japonica, Tripterospermum japonicum, Vaccinium uliginosum, Vaccinium vitis-idaea, etc. Especially, plants like, Acanthopanax senticosus, Jeffersonia dubia and Lillum cernum which have high values of ornamental, and medical use are in an extinction crisis. High swampy land was formed to upper stream of Tumen river. It showed representative vegitation, like Carex sp., Scirpus radirans, Eriophorum latifolium. Wetlands plant and a dune plant were mixed in down stream area of Tumen river. On a dune, seashore plants, such as Carex kobomugi, Salsola collina, Raphanus sativus var. raphanistroides, Rosa rugosa, Scutellaria strigillosa, Linaria japonica, Phacelurus latifolius were appeared frequently.