• Bulletin of the Korean Chemical Society
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• v.32 no.2
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• pp.681-686
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• 2011

SPLITT Fractionation (SF) provides separation of sample into two subpopulations. Separation into more than two subpopulations requires repeated SF operations. In this study, two Gravitation SF (GSF) channels were connected in a series (Tandem GSF) to obtain a separation into three subpopulations and to improve the fractionation efficiency (FE) of the fraction-b in the full-feed depletion (FFD) mode. In a single channel FFD-GSF operation, the fraction-a contained mostly the beads smaller than the cutoff diameter ($d_c$), while the fraction-b contained beads smaller than $d_c$ as well as those larger than dc, as expected. The measured FE's of the fraction-b are much lower than those of the fraction-a in all cases. The FE's of the fraction-a are higher than 84% with the average of about 91%, while those of the fraction-b are lower than 60% with the average of about 43%. No particular trends were found between FE and $d_c$, indicating the performance of FFD-GSF does not change with $d_c$ in the range where tested. Also no clear trends were observed between the FE and the sample-feeding flow rate, indicating higher sample-feeding rate can be used to increase the sample throughput without losing resolution. When two GSF channels were connected so that the flow stream emerging from the outlet-b of the channel-1 is fed directly into the channel-2, all three FE's measured for the fraction-1a were high with the average value of 99%, indicating it contains almost purely the beads smaller than $d_c$. The FE's measured for the fraction-2a are still good with the average value of 92%. The FE's measured for the fraction-2b are 64% in average, which is about 20% improvement from those obtained in a single channel FFD-GSF at the same conditions.

• Journal of Korean Port Research
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• v.13 no.2
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• pp.333-350
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• 1999

Today freeway is thought to be a very important transportation facility carrying tremendous traffic flow as the main corridor within the area of between the areas. However freeway is experiencing severe congestion and accidents by increased entrance ramp flow especially at peak time period. Ramp meters on the freeway entrance ramps that supply traffic to the freeway in a measured or appropriately regulated amount are needed for alleviating freeway congestion. Because ramp meters can be operated to discharge traffic at a measured or regulated rate thus maintaining more uniform speed on the mainline section maximizing the throughput to the freeway within the capacity of a downstream bottleneck and reducing the congestion related accidents. Thus the objectives in this study were to analyze the traffic characteristics on the freeway I-94 with ramp metering system before/after ITS technology in Detroit (Michigan) area compare shifts of the traffic characteristics on the freeway I-94 before/after ITS technology and finally suggest a better ramp metering strategy for the freeway system The following results were obtained: i)Flow occupancies and speeds on the mainline merge section of freeway were shown to be a big difference depending on the peak periods areas and directions based on the distribution of traffic flow characteristics on the freeway. ii)Reduced speed was shown to be more than 5 mph and ramp flow was also shown to be more than 240 vph at peak periods if there was the ramp metering system constructed on the freeway. iii)Ramp metering system was shown to be optimally operated on the freeway if ramp flow could be maximized within the range of over 900 vph and reduced occupancy could be also maximized by no more than 2 percent at peak periods. iv)The average flows on the freeway after the ITS technology were shown to be a decrease of over 20% depending on the peak periods areas and directions when compared with those flow on the freeway before the ITS technology. over 20% depending on the peak periods areas and directions when compared with those speeds on the freeway before the ITS technology. vi)The average metering rates on the freeway after the ITS technology were shown to be an increase of over 10% depending on the peak periods areas and directions when compared with those metering rates on the freeway before the ITS technology.

• Analytical Science and Technology
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• v.27 no.1
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• pp.34-40
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• 2014

SPLITT fractionation (SF) allows continuous (and thus a preparative scale) separation of micronsized particles into two size fractions ('fraction-a' and 'fraction-b'). SF is usually carried out in a thin rectangular channel with two inlets and two outlets, which is equipped with flow stream splitters at the inlet and the outlet of the channel, respectively. A new large scale splitter-less gravitational SF (GSF) system had been assembled, which was designed to eliminate the flow stream splitters and thus is operated by the full feed depletion (FFD) mode (FFD-GSF). In the FFD mode, there is only one inlet through which the sample is fed. There is no carrier liquid fed into the channel, and thus prevents the sample dilution. The effects of the sample-feeding flow rate, the channel thickness on the fractionation efficiency (FE, number % of particles that have the size predicted by theory) of FFD-GSF was investigated using industrial polyurethane (PU) latex beads. The carrier liquid was water containing 0.1% FL-70 (particle dispersing agent) and 0.02% sodium azide (used as bactericide). The sample loading rate was varied from about 4 to 7 L/hr with the sample concentration fixed at 0.01%. The GSF channel thickness was varied from 900 to $1300{\mu}m$. Particles exiting the GSF channel were collected and monitored by optical microscopy (OM). Sample recovery was monitored by collecting the fractionated particles on a $0.45{\mu}m$ membrane filter. It was found that FE of fraction-a was increased as the channel thickness increases, and FE of fraction-b was increased as the flow rate was increased. In all cases, the sample recovery has higher than 95%. It seems the new splitter-less FFD GSF system could become a useful tool for large scale separations of various types of micron-sized particles.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.18 no.12
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• pp.1970-1982
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• 1993

This paper describes an efficient array algorithm for parallel computation of vector-radix two-dimensional (2-D) fast discrete cosine transform (VR-FCT), and its VLSI implementation. By mapping the 2-D VR-FCT onto a 2-D array of processing elements (PEs), the butterfly structure of the VR-FCT can be efficiently importanted with high concurrency and local communication geometry. The proposed array algorithm features architectural modularity, regularity and locality, so that it is very suitable for VLSI realization. Also, no transposition memory is required, which is invitable in the conventional row-column decomposition approach. It has the time complexity of O(N+Nnzp-log2N) for (N*N) 2-D DCT, where Nnzd is the number of non-zero digits in canonic-signed digit(CSD) code, By adopting the CSD arithmetic in circuit desine, the number of addition is reduced by about 30%, as compared to the 2`s complement arithmetic. The computational accuracy analysis for finite wordlength processing is presented. From simulation result, it is estimated that (8*8) 2-D DCT (with Nnzp=4) can be computed in about 0.88 sec at 50 MHz clock frequency, resulting in the throughput rate of about 72 Mega pixels per second.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.11
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• pp.44-52
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• 1998

In this paper, we proposed a node architecture and cell routing strategies for ATM applications in WDM multihop networks. The proposed node architecture employs the optical delay loop for storing the cell which is failed in out-link contention. This optical delay loop allows the delay of one cell without the electro-optic conversion. Therefore, we can get the advantages of S&F(Store-and-Forward) routing in Deflection-based all-optical networks. To support the ATM applications efficiently. we considered the transmission priority of ATM cell so that high priority cell can be transmitted with lower loss and shorter delay than low priority one. Two kinds of routing strategies are designed for this architecture: Scheme-Ⅰand Scheme-Ⅱ. Scheme-Ⅰapplies S&F routing to high cell and Deflection routing to low cell, i.e., high cells are routed along the shortest path based on S&F routing, but low cells are deflected or lost. Schem-Ⅱ is similar to Scheme-Ⅰexcept that low cells can occupy the optical loop if it is available. This Scheme-Ⅱ increases the utilization of network resources without decreasing the throughput of high cell by reducing the low cell loss rate when traffic load is low. Simulation results show that our routing strategies have better performance than conventional ones under non-uniform traffic as well as uniform traffic.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.253-259
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• 2016

In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost- and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, $20{\mu}l$ in 1 sample, was optimal, and the parasite density as low as $2p/{\mu}l$ for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings (<9% positive rate). The results indicated that pooling of the blood samples before DNA extraction followed by usual nested PCR is useful and effective for detection of malaria in screening of hidden cases in low-transmission settings.

• The KIPS Transactions:PartC
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• v.14C no.4
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• pp.349-358
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• 2007

Ethernet Passive Optical Network(EPON), which is one of PON technologies for realizing FTTx(Fiber-To-The-Curb/Home/Office), can cost-effectively construct optical access networks. In addition, EPON can provide high transmission rate up to 10Gbps and it is compatible with existing customer devices equipped with Ethernet card. To effectively control frame transmission from ONUs to OLT EPON can use Multi-Point Control Protocol(MPCP) with additional control functions in addition to Media Access Control(MAC) protocol function. For EPON, many researches on intra- and inter-ONU scheduling algorithms have been performed. Among the inter-ONU scheduling algorithms, IPS(Interleaved Polling with Stop) based on polling scheme is efficient because OLT assigns available time portion to each ONU given the request information from all ONUs. Since the IPS needs an idle time period on uplink between two consecutive frame transmission periods, it wastes time without frame transmissions. In this paper, we propose a dynamic bandwidth allocation algorithm to increase the channel utilization on uplink and evaluate its performance using simulations. The simulation results show that the proposed Two-phase Cycle Danamic Bandwidth Allocation(TCDBA) algorithm improves the throughput about 15%, compared with the IPS and Fast Gate Dynamic Bandwidth Allocation(FGDBA). Also, the average transmission time of the proposed algorithm is lower than those of other schemes.

• The KIPS Transactions:PartC
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• v.17C no.3
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• pp.271-280
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• 2010

For the next generation mobile communication system, diverse wireless network techniques such as beyond 3G LTE, WiMAX/WiBro, and next generation WLAN etc. are proceeding to the form integrated into the All-IP core network. According to this development, Beyond 3G integrated into heterogeneous wireless access technologies must support the vertical handover and network to be used of several radio networks. However, unified management of each network is demanded since it is individually serviced. Therefore, in order to solve this problem this study is introducing the theory of Common Radio Resource Management (CRRM) based on Generic Link Layer (GLL). This study designs the structure and functions to support the vertical handover and propose the vertical handover algorithm of which policy-based and MCDM are composed between LTE and WLAN systems using GLL. Finally, simulation results are presented to show the improved performance over the data throughput, handover success rate, the system service cost and handover attempt number.

• KIPS Transactions on Computer and Communication Systems
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• v.2 no.9
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• pp.381-392
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• 2013

During the last decade, the multitude of advances attained in terminal computers, along with the introduction of mobile hand-held devices, and the deployment of high speed networks have led to a recent surge of interest in Quality of Service (QoS) for video applications. The main difficulty is that mobile devices experience disparate channel conditions, which results in different rates and patterns of packet loss. One way of making more efficient use of network resources in video services over wireless channels with heterogeneous characteristics to heterogeneous types of mobile device is to use a scalable video coding (SVC). An SVC divides a video stream into a base layer and a single or multiple enhancement layers. We have to ensure that the base layer of the video stream is successfully received and decoded by the subscribers, because it provides the basis for the subsequent decoding of the enhancement layer(s). At the same time, a system should be designed so that the enhancement layer(s) can be successfully decoded by as many users as possible, so that the average QoS is as high as possible. To accommodate these characteristics, we propose an efficient transmission scheme which incorporates SVC-aware dual-channel repetition to improve the perceived quality of services. We repeat the base-layer data over two channels, with different characteristics, to exploit transmission diversity. On the other hand, those channels are utilized to increase the data rate of enhancement layer data. This arrangement reduces service disruption under poor channel conditions by protecting the data that is more important to video decoding. Simulations show that our scheme safeguards the important packets and improves perceived video quality at a mobile device.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.