• Title/Summary/Keyword: Three-Shift

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Serum Free Medium Development for Recombinant Erythropoietin Production using Novel Cell Line (QT35) (QT35 세포주에서 제조합 에리스로포이에틴 생산을 위한 무혈청 배지의 개발)

  • 주형민;김병기;김선영;김태한;김태용
    • KSBB Journal
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    • v.13 no.3
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    • pp.295-302
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    • 1998
  • Human Erythropoietin (EPO) gene is cloned in quail fibrosarcoma cell, QT35. Because molecular weight of EPO is similar to that of serum albumin, cell culture with serum containing medium makes purification of EPO very difficult. Using fractional factorial study, we have developed serum free medium for the recombinant QT35 cell lines, QT N4D4 and QT SY-IMP, which have cloned EPO with glutamine synthetase (GS) gene amplification system and with puromycin selective marker, respectively. Among the seven frequently used medium components, fibronectin, BSA, and EGF were the most important for EPO production. However, sufficient fibronectin supplement to the medium did not make any good attachment of QT35 to culture plate over 3 days. Therefore, to maximize EPO production, we attempted a medium-shift at confluence from serum containing medium to serum free medium(QT SFM6). Using the medium-shift protocol with QT SFM6, nearly the same productivity of EPO was achieved comparing with that without medium-shift. This result was true in both QT35 cell lines in three types of culture, i.e. T flask, microcarrier and roller bottle cultures.

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Statistical comparison of morphological dilation with its equivalent linear shift-invariant system:case of memoryless uniform soruces (무기억 균일 신호원에 대한 수리 형태론적인 불림과 등가 시스템의 통계적 비교)

  • 김주명;최상신;최태영
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.34S no.2
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    • pp.79-93
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    • 1997
  • This paper presents a linear shift-invariant system euqivalent to morphological dilation for a memoryless uniform source in the sense of the power spectral density function, and comares it with dialtion. This equivalent LSI system is found through spectral decomposition and, for dilation and with windwo size L, it is shown to be a finite impulse response filter composed of L-1 delays, L multipliers and three adders. Th ecoefficients of the equivalent systems are tabulated. The comparisons of dilation and its equivalent LSI system show that probability density functions of the output sequences of the two systems are quite different. In particular, the probability density functon from dilation of an independent and identically distributed uniform source over the unit interval (0, 1) shows heavy probability in around 1, while that from the equivalent LSI system shows probability concentration around themean vlaue and symmetricity about it. This difference is due to the fact that dilation is a non-linear process while the equivalent system is linear and shift-ivariant. In the case that dikation is fabored over LSI filters in subjective perforance tests, one of the factors can be traced to this difference in the probability distribution.

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Identification of Inrush and Internal Fault in Indirect Symmetrical Phase Shift Transformer Using Wavelet Transform

  • Bhasker, Shailendra Kumar;Tripathy, Manoj;Kumar, Vishal
    • Journal of Electrical Engineering and Technology
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    • v.12 no.5
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    • pp.1697-1708
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    • 2017
  • This paper proposes an algorithm for the differential protection of an Indirect Symmetrical Phase Shift Transformer (ISPST) by considering the different behaviors of the compensated differential current under internal fault and magnetizing inrush conditions. In this algorithm, a criterion function is defined which is based on the difference of amplitude of the wavelet transformation over a specific frequency band. The function has been used for the discrimination between three phase magnetizing inrush and internal fault condition and requires less than a quarter cycle after disturbance. This method is independent of any coefficient or threshold values of wavelet transformation. The merit of this algorithm is demonstrated by the simulation of different faults in series and excitation unit and magnetizing inrush with varying switching conditions on ISPST using PSCAD/EMTDC. Due to unavailability of in-field large interconnected transformers for such a large number of destructive tests, the results are further verified by Real Time Digital Simulator (RSCAD/RTDS). The proposed algorithm has been compared with the conventional harmonic restraint based method that justifies the application of wavelet transform for differential protection of ISPST. The proposed algorithm has also been verified for different rating of ISPSTs and satisfactory results were obtained.

The Three Directional Separable Processing Method for Double-Density Wavelet Transformation Improvement (이중 밀도 웨이브렛 변환의 성능 향상을 위한 3방향 분리 처리 기법)

  • Shin, Jong Hong
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.8 no.2
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    • pp.131-143
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    • 2012
  • This paper introduces the double-density discrete wavelet transform using 3 direction separable processing method, which is a discrete wavelet transform that combines the double-density discrete wavelet transform and quincunx sampling method, each of which has its own characteristics and advantages. The double-density discrete wavelet transform is nearly shift-invariant. But there is room for improvement because not all of the wavelets are directional. That is, although the double-density DWT utilizes more wavelets, some lack a dominant spatial orientation, which prevents them from being able to isolate those directions. The dual-tree discrete wavelet transform has a more computationally efficient approach to shift invariance. Also, the dual-tree discrete wavelet transform gives much better directional selectivity when filtering multidimensional signals. But this transformation has more cost complexity Because it needs eight digital filters. Therefor, we need to hybrid transform which has the more directional selection and the lower cost complexity. A solution to this problem is a the double-density discrete wavelet transform using 3 direction separable processing method. The proposed wavelet transformation services good performance in image and video processing fields.

Analysis of Characteristics of Chungnam Area Tourism Industrial Structure by Dynamic Shift-Share Analysis (동태적 변이할당분석을 이용한 충남지역 관광산업 구조 특성 분석)

  • Choi, Seung-Mook
    • The Journal of the Korea Contents Association
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    • v.15 no.3
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    • pp.396-405
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    • 2015
  • Based on the number of employees and sales of tourism establishments in Chungnam ares for the period from 2009 to 2013, this study analyzes the regional growth characteristic of tourism industry using dynamic shift-share analysis. The data used for this analysis are obtained from "The Census on Basic Characteristic of Tourism Establishments". This study decomposes characteristics of Chungnam Area tourism industrial structure into three components: national growth effect, industrial mix effect and regional share effect. The results help to well understand the development and the competitive situation of Chungnam area tourism industry.

A Historical Study on the Paradigm Shift of Environment Subject Curriculum in Korea (한국 환경과 교육 과정의 패러다임 변화에 대한 역사적 고찰)

  • Lee, Soon-Chul;Choi, Don-Hyung
    • Hwankyungkyoyuk
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    • v.23 no.1
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    • pp.27-35
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    • 2010
  • The purpose of this study is to find historical paradigm shift of the environmental subject in Korean school curriculum. For the purpose, two research questions guided this study. First, we would like to find out the changes in environmental education curriculum by interpreting the Korean national curriculum. Second, we want to know about the paradigm shift of environment education in Korea. In this study, curriculum documents, teachers' guide books, textbooks, and other educational materials were used for literature review. The results are as follows: First, Robottom and Hart's frame of three paradigms in environmental education research, positivism, interpretivism and social criticism, was suitable in interpreting curriculum. Second, the curriculum of environment subject has substantially changed from 6th to 2007 revised curriculum. Third, while the 6th curriculum was strongly affected by the positivism and education 'about' the environment, the 7th curriculum had been influenced by the interpretivism and education 'in/through' the environment, and the 2007 revised curriculum is under the influence of the education 'for' the environment and social critical paradigm.

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Task-specific Noise Exposure Assessment of Firefighters

  • Kang, Taesun
    • Journal of Environmental Health Sciences
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    • v.45 no.6
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    • pp.569-576
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    • 2019
  • Objectives: The main purpose of this study was to assess firefighters' daily personal noise exposure and explore noise levels related to specific tasks and their contributions to total noise exposure using 24-hour full-shift noise exposure measurements with task-based data. Methods: Noise exposure was assessed for eight firefighters (two rescuers, two drivers, and four suppressors) using time-activity diaries. We collected a total of 24 full-shift personal noise sample sets (three samples per a firefighter). The 24-hour shift-adjusted daily personal noise exposure level (Lep,d), eight weekly personal noise exposures (Leq,w), and 40 task-specific Leq values (Leq activity) were calculated via the ISO/NIOSH method. Results: The firefighter noise-sample datasets showed that most firefighters are exposed to noise levels above EU recommended levels at a low-action value. The highest noise exposure was for rescuers, followed by drivers and suppressors. Noise measurements with time-at-task information revealed that 82.3% of noise exposure occurred when checking equipment and responding to fire or emergency calls. Conclusions: The results indicate that firefighters are at risk of noise-induced hearing loss. Therefore, efforts at noise-control are necessary for their protection. This task-specific noise exposure assessment also shows that protective measures should be focused on certain tasks, such as checking and testing equipment.

Self-calibration Algorithm of Systematic Errors For Interferometer (간섭계에 있어서의 계통 오차의 자율 교정 알고리즘)

  • Ikumatsu Fujimoto;Lee Taeyong
    • Journal of the Korean Society for Precision Engineering
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    • v.22 no.5 s.170
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    • pp.63-71
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    • 2005
  • When an almost flat surface under test is measured by an interferometer, the measurement result is largely influenced by systematic errors that include geometrical errors of a reference flat surface. To determine the systematic errors of the interferometer by the conventional method that is called the three flat method, we must take the reference flat surface out from the interferometer and measure it. Because of difficulties to set the reference flat surface to the interferometer exactly and quickly, this method is not practical. On the other hand, the method that measures a surface under test with some shifts in the direction being perpendicular to the optical axis of the interferometer is studied. However, the parasitic pitching, rolling and up-down movement caused by the above shifts brings serious error to the measurement result, and the algorithm by which the influences can be eliminated is not still established. In this paper, we propose the self-calibration algorithm for determining the systematic errors that include geometrical errors of a reference flat surface by several rotation shifts and a linear shift of general surface under test, and verify by a numerical experiment that this algorithm is useful for determining the systematic errors.

Predictive Factors of Health promotion behaviors of Industrial Shift Workers (산업장 교대근무 근로자의 건강증진행위 예측요인)

  • Kim, Young-Mi
    • Korean Journal of Occupational Health Nursing
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    • v.11 no.1
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    • pp.13-30
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    • 2002
  • Industrial shift workers feels suffer mental stresses which are caused by unfamiliar day sleep, noisy environment, sleeping disorder by bright light, unusual contacts with family, difficulty in meeting with friends or having formal social meetings and other social limitations such as the use of transportation. Such stresses influence health of the workers negatively. Thus the health promotion policy for shift workers should be made considering the workers' ways of living and shift work specially. This study attempted to provide basic information for development of the health promotion program for industrial shift workers by examining predictive factors influencing health promotion behaviors of those workers. In designing the study, three power generation plants located in Pusan and south Kyungsang province were randomly selected and therefrom 280 workers at central control, boiler and turbine rooms and environmental chemistry parts whose processes require shift works were sampled as subjects of the study. Data were collected two times from September 17 to October 8, 1999 using questionnaires with helps of safety and health managers of the plants. The questionnaires were distributed through mails or direct visits. Means for the study included the measurement tool of health promotion behavior provided by Park(1995), the tool of self-efficacy measurement by Suh(1995), the tool of internal locus of control measurement by Oh(1987), the measurement tool of perceived health state by Park(1995) and the tool of social support measurement by Paek(1995). The collected data were analyzed using SPSS program. Controlling factors of the subjects were evaluated in terms of frequency and percentage ratio Perceived factors and health promotion behaviors of the subjects were done so in terms of mean and standard deviation, and average mark and standard deviation, respectively. Relations between controlling and perceived factors were analyzed using t-test and ANOVA and those between perceived factors and the performance of health promotion behaviors, using Pearson's Correlation Coefficient. The performance of health promotion behaviors was tested using t-test, ANOVA and post multi-comparison (Scheffe test). Predictive factors of health promotion behavior were examined through the Stepwise Multiple Regression Analysis. Results of the study are summarized as follows. 1. The performance of health promotion behaviors by the subjects was evaluated as having the value of mean, $161.27{\pm}26.73$ points(min.:60, max.:240) and average mark, $2.68{\pm}0.44$ points(min.:1, max.:4). When the performance was analyzed according to related aspects, it showed the highest level in harmonious relation with average mark, $3.15{\pm}.56$ points, followed by hygienic life($3.03{\pm}.55$), self-realization ($2.84{\pm}.55$), emotional support($2.73{\pm}.61$), regular meals($2.71{\pm}.76$), self-control($2.62{\pm}.63$), health diet($2.62{\pm}.56$), rest and sleep($2.60{\pm}.59$), exercise and activity($2.53{\pm}.57$), diet control($2.52{\pm}.56$) and special health management($2.06{\pm}.65$). 2. In relations between perceived factors of the subjects(self-efficacy, internal locus of control, perceived health state) and the performance of health promotion behaviors, the performance was found having significantly pure relations with self-efficacy (r=.524, P=.000), internal locus of control (r=.225, P=.000) and perceived health state(r=.244, P=.000). The higher each evaluated point of the three factors was, the higher the performance was in level. 3. When relations between the controlling factors(demography-based social, health-related, job-related and human relations characteristics) and the performance of health promotion behaviors were analyzed, the performance showed significant differences according to marital status (t=2.09, P= .03), religion(F=3.93, P= .00) and participation in religious activities (F=8.10, P= .00) out of demography-based characteristics, medical examination results (F=7.20, P= .00) and methods of the collection of health knowledge and information(F=3.41, P= .01) and methods of desired health education(F=3.41, P= .01) out of health-related characteristics, detrimental factors perception(F=4.49, P= .01) and job satisfaction(F=8.41, P= .00) out of job-related characteristics and social support(F=14.69, P= .00) out of human relations characteristics. 4. The factor which is a variable predicting best the performance of health promotion behaviors by the subjects was the self-efficacy accounting for 27.4% of the prediction, followed by participation in religious activities, social support, job satisfaction, received health state and internal locus of control in order all of which totally account for 41.0%. In conclusion, the predictive factor which most influence the performance of health promotion behaviors by shift workers was self-efficacy. To promote the sense, therefore, it is necessary to develop the nursing intervention program considering predictive factors as variables identified in this study. Further industrial nurses should play their roles actively to help shift workers increase their capability of self-management of health.

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The Movement of Foot and the Shift of Ground Reaction Force in Batters according to the Ball Speed Increase (투구 속도 증가에 따른 타자의 발 움직임과 지면 반력의 변화)

  • Lee, Young-Suk;Eun, Seon-Deok
    • Korean Journal of Applied Biomechanics
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    • v.14 no.3
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    • pp.191-202
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    • 2004
  • The batting performance in baseball is a repetitive movement. In order to make the stabilization of posture and the efficient shift of body weight, both the range of stance and stride are important. The previous studies explained that the consistent stride which included the amount of time, stance, and direction were needed. However, the batting performance is frequently changed according to the several speed of ball. Therefore, this study was to analyze the reaction time, the range of stance, the change of stride, and the change of GRF during the batting movement in three kinds of ball speed (120km/h, 130km/h, & 140km/h). Seven elite players are participated in this study. 1. The reaction time of the stride phase was short whereas the time of the swing phase was long according to the increasing ball speed. 2. The range of the stance was wide and the mediolateral direction of the stride was decreased according to the increasing ball speed. 3. In the three kinds of ball speed, the change of body weight was transferred to the center, the rear foot, and the front foot directions. The ball speed of 130km/h showed the high frequency of the suitable batting. At this ball speed, the movement of the body weight was shifted smoothly and the value of the Ground Reaction Force was large enough.