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DNA barcoding of Schisandraceae in Korea (한국산 오미자과의 DNA 바코드)

  • Youm, Jung Won;Han, Sang-Wook;Seo, Seon Won;Lim, Chae Un;Oh, Sang-Hun
    • Korean Journal of Plant Taxonomy
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    • v.46 no.3
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    • pp.273-282
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    • 2016
  • The establishment of a DNA barcode database at the regional scale and assessments of the utility of DNA barcodes are crucial for conservation biology and for the sustainable utilization of biological resources. Schisandraceae is a small family consisting of ca. 45 species. It contains many economically important species, such as Schisandra chinensis, which is widely used as a source in tonic beverages and in oriental medicine. In Korea, three species, S. chinensis, S. repanda, and Kadsura japonica, are distributed. We evaluated the level of variation of the DNA sequences of rbcL, matK, and the ITS regions from 13 accessions representing the distributional range of the three species. The three DNA barcode regions were easily amplified and sequenced. The minimum values of the interspecific genetic distances among S. chinensis, S. repanda, and K. japonica either separately or in combination are 4- to 23-fold higher than the maximum value of the intraspecific distance, showing that there is a clear DNA barcoding gap in the regions for Korean Schisandraceae. Phylogenetic analyses of the three DNA barcode regions, separately and simultaneously, indicate that all of the DNA barcode regions are useful for identifying a species of Schisandraceae in Korea. The distinctiveness of the three species of Schisandraceae was also supported at the species level when Chinese and Japanese populations were added. The results of this study indicate that three concatenated regions constitute the best option for DNA barcoding in Schisandraceae in Korea.

A STUDY ON THE SHAPE OF A CANAL PREPARED WITH 'THREE-FILE' TECHNIQUE IN A CURVED CANAL ('Three-File' 방식에 의한 만곡 근관 형성시 근관의 형태에 관한 연구)

  • Park, Han-Soo;Lee, Min-Koo;Kim, Jong-Jin;Lim, Young-Jun;Jang, Moon-Sung;Lee, Jong-Yeop
    • Restorative Dentistry and Endodontics
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    • v.25 no.4
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    • pp.494-498
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    • 2000
  • 이 연구의 목적은 'Three-File' 방식에 의한 근관형성시 근관의 형태변화 및 전이정도를 분석하고 이를 기존의 근관형성방법과 비교하고자 하는 것이다. 만곡된 근관을 가진 투명한 레진블럭 상에서 'Three-File' 방식, crown-down방식 및 스테인레스강 화일에 의한 수동방식으로 근관형성을 시행하고 이를 똑같은 위치가 재현가능한 고정틀에 위치시킨 후 사진의 이중노출 기법을 이용하여 근관형성 전후의 근관의 형태변화 및 전이정도를 분석한 결과 다음과 같은 결과를 얻었다. 1. 스테인레스강 화일의 경우 근관확대가 가장 컸고 근관의 전이정도도 심하였으며, 형성된 근관이 taper하지 못하고 불규칙한 형태를 보였다. 2. Profile로 crown-down방식에 의해 형성한 경우 taper한 근관형태를 보였고, 근관의 전이 정도도 스테인레스강 화일의 경우보다 작았다(p<0.01). 3. Profile로 'Three-File'방식에 의해 형성한 경우 taper한 근관형태를 보였고 근관의 전이정도도 스테인레스강 화일의 경우보다 유의하게 작았으나(p<0.01), crown-down방식에 의해 형성한 경우와 유의할 만한 차이는 없었다(p>0.01).

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A study on nail art design based on the application of trimmings in the 18th century women's clothes - Focus on braids, laces, and ribbons - (18세기 여성 의복에 나타난 트리밍을 응용한 네일아트 디자인 연구 - 브레이드, 레이스, 리본을 중심으로 -)

  • Cho, Han-Sol;Rhee, Young-Ju
    • Journal of the Korea Fashion and Costume Design Association
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    • v.21 no.4
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    • pp.127-137
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    • 2019
  • Nail art, which has been popularized recently, has increased convenience in the direction of nail art design harmonized with clothing and the increased interest and usage of nail art decoration. This study set out to propose practical planar and three-dimensional nail art designs in harmony with modern clothes by applying trimmings common in 18th century women's clothing, which used a variety of attachable decorations. As for the methodology, the investigator examined theories in previous studies, literature, and analyzed the types of trimming in female portraits from the 18th century at the museum at the Palace of Versailles in France. Planar and three-dimensional nail art designs were created for each of the top three trimming types- braids, laces, and ribbons, which were identified through analysis. The study applied the trimmings of the 18th century women's clothes to nail art design and had the following results: First, the top three trimming types were identified based on the analysis of the 18th century portraits. They were then used as motifs in the production of planar and three-dimensional nail art designs. The results show that the motifs of the clothes trimmings were fit for and had practical possibilities for expression in nail art designs. Secondly, diversity and the expansion of ideas in the study of nail art design were promoted. In addition, elements were increased to have mutually complementary effects with clothes. Finally, the study made various attempts at nail design methods by adopting a range of nail art expression techniques including hand painting, 2D & 3D art techniques, and using planar and three-dimensional nail decorations, thus proposing a positive direction for the organization of a curriculum to make use of 18th century women's clothing motifs in nail art education.

THE THREE DIMENSIONAL FINITE ELEMENT ANALYSIS OF THE STRESS DISTRIBUTION IN THE THREE TREATMENT OPTIONS OF IMPLANTS RESTORATIONS FOR THE POSTERIOR PARTIAL EDENTULISM (구치부 부분 무치악 결손에서 유용한 세 가지 임프란트 수복법들의 응력분산에 관한 3차원 유한요소법적 비교연구)

  • Kim, Il-Kyu;Lee, Hyeung-Uk;Ryu, Seung-Hyun;Choi, Jin-Ho;Han, Ye-Sook;Son, Choong-Yul;Byun, Hyo-In
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.30 no.3
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    • pp.175-180
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    • 2004
  • In this study, three treatment options to replace two posterior missing teeth were investigated using three dimensional finite element analysis: two wide(${\phi}5.0mm$) implants(the experimental model I), two standard(${\phi}3.75mm$) implants(the experimental model II), and three standard(${\phi}3.75mm$) implants(the experimental model III). Two kinds of load case were applied ; 1) perpendicular on occlusal surface(axial load), parallel on occlusal surface(lateral load). 2) perpendicular on occlusal surface(3mm lateral to central point). The results obtained from this study were as follows; value of Von-mises stress (equivalent stress) was smallest in the two wide implant among the three experimental models. It was reported that the diameter is the efficient factor than osseointegrated surface area.

The Analysis of Operation Mode of Three-Phase Flux-Lock Type Superconducting Fault Currents Limiter (삼상 자속구속형 한류기의 동작모드분석)

  • Hwang, Jong-Sun;Choi, Hyo-Sang;Park, Hyoung-Min;Cho, Youn-Sun;Lee, Na-Young;Nam, Gueng-Hyun;Han, Tea-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.05a
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    • pp.46-48
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    • 2006
  • We investigated the analysis of operation mode of three-phase flux-lake type superconducting fault current limiter(SFCL). The structure of the integrated three-phase flux-lock type SFCL consisted of three-phase flux-lock reactor wound on an iron core with the same turn between coil 1 and coil 2 in each phase. When the SFCL is operated under the normal condition, the flux generated in the iron core is zero because the flux generated between two coils of each single phase is canceled out. Therefore, the SFCL's impedance is zero and the SFCL has negligible influence on the power system, However, if a fault occurs in any single-phase among three phases, the flux generated in the iron core is not zero any more. The flux makes elements of all phase-quench irrespective of the fault type, which reduces the current of fault phase as well as the current of sound phase.

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The Development of Rotoless Electronic Circulating Pump System with Internal Three Way Valve for Boiler - Objective for the 1st Year : Development of New Housing with Internal Three Way Valve - (보일러용 3way valve내장형 rotoless 전자식순환펌프 시스템의 개발 - 1차년도 목표 : 3way valve 내장형 하우징의 개발 -)

  • Han, J.W.;Kum, S.M.;Ryu, B.H.;Lee, C.E.;Ohu, S.C.;Yim, J.S.
    • Solar Energy
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    • v.16 no.2
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    • pp.97-112
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    • 1996
  • The purpose of research is to complement the circulating pump of gas boiler for the domestic and to develop a pump housing with internal three way valve: Housing and three way valve have been apart in the existing pump system. Based on the experimental result on can-typed circulating pump with existing housing, a new housing with internal three way valve was designed and manufactured. The performance of can-typed circulating pump with the new housing, and the performance of circulating pump of boiler system were tested. As a result ot the test, the new housing with internal three way valve has been excellent in respect of pump performance, weight and manufacturing cost. So It is expected to have an effect of import substitution.

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Adventitious Root Development and Ginsenoside Production in Panax ginseng, Panax quinquefolium and Panax japonicum

  • Han, Jung-Yeon;Kwon, Yong-Soo;Choi, Yong-Eui
    • Journal of Plant Biotechnology
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    • v.33 no.2
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    • pp.147-152
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    • 2006
  • This work was carried out to establish adventitious root culture system in three Panax species (wild-grown P. ginseng, P. quinquefolium, and P. japonicum) to analyze their ginsenoside productivity. Adventitious roots were induced directly from segments of seedlings after cultured on MS(Murashige andSkoog 1962) solid medium containing 3.0 mg/l IBA. Omission of $NH_4NO_3$ from the medium greatly enhanced both the frequency of adventitious root formation and number of roots per explants in all the three Panax species. However, elongation of post-induced adventitious roots was enhanced on medium with $NH_4NO_3$. Two-step culture protocol: $NH_4NO_3$-free medium for first two weeks of culture, followed by $NH_4NO_3$ containing medium for further 4 weeks, greatly enhanced the fresh weight increase of adventitious roots in all the three ginseng species. The fresh weight of adventitious roots was high in P. quinquefolium and low in P. ginseng, followed by P. japonioum regardless of the composition of medium. Pattern and content of ginsenosides in adventitious roots differed among the three Panax species. Total ginsenoside content of adventitious roots in P. quinquefolium, P. ginseng, and p. japonicum was 8.03, 15.7 and 1.2 mg/g dry weight, respectively. Among the three speices, adventitious roots in P. quinquefolium produced hig-hamount of ginsenosides. The pattern of ginsenoside fractions between P. ginseng and P. quinquefolium was similar but the amount of ginsenoside differed between the two, While, in P japonicum, total ginsenoside content was very low and some ginsenosides such as ginsenoside Rb2 and Rf were not detected. Conclusively, we demonstrate that same culture condition was required for induction and elongation of adventitious roots of three ginseng species but growth of adventitious roots and their ginsenoside production were different among them.

Design and Implementation of a 3D Pointing Device using Inertial Navigation System (관성항법시스템을 이용한 3D 포인팅 디바이스의 설계 및 구현)

  • Kim, Hong-Sop;Yim, Geo-Su;Han, Man-Hyung;Lee, Keum-Suk
    • Journal of the Korea Society of Computer and Information
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    • v.12 no.5
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    • pp.83-92
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    • 2007
  • In this paper, we present a design and implementation of three dimensional pointing device using Inertial Navigation System(INS) that acquires coordinates and location information without environmental dependancy. The INS measures coordinates based on the data from gyroscope and accelerometer and corrects the measured data from accelerometer using Kalman-Filter. In order to implement the idea of three dimensional pointing device, we choose a three dimensional Space-recognition mouse and use RFIC wireless communication to send a measured data to receiver for printing out the coordinate on display equipment. Based on INS and Kalman-Filter theoretical knowledge, we design and implement a three dimensional pointing device and verified the usability as an input device that can capture a human's move. also, we describe the applicability of this device in ubiquitous computing environment.

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Real-Time Color Gamut Mapping Method Based on the Three-Dimensional Difference Look-Up Table (3차원 차분 룩업 테이블을 이용한 실시간 색역 사상 기법)

  • Han, Dong-Il
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.42 no.6
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    • pp.111-120
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    • 2005
  • A cost effective three-dimensional color gamut mapping architecture is described. The conventional three-dimensional reduced resolution look-up table is considered and the concept of three-dimensional reduced resolution difference look-up table is introduced for cost effective and real-time color gamut mapping. The overall architecture uses one-dimensional memory decomposition of three-dimensional gamut mapping look-up table, three-dimensional interpolation and simple addition operation for generating the final gamut mapped colors. The required computational cost is greatly reduced by look-up table resolution adjustment and further reduced by the gamut mapping rule modification. The proposed architecture greatly reduces the required memory size and hardware complexity compared to the conventional method and it is suitable for real-time applications. The proposed hardware is suitable for FPGA and ASIC implementation and could be applied to the real-time display quality enhancement purposes.

Study on Aerodynamic Optimization Design Process of Multistage Axial Turbine

  • Zhao, Honglei;Tan, Chunqing;Wang, Songtao;Han, Wanjin;Feng, Guotai
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2008.03a
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    • pp.130-135
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    • 2008
  • An aerodynamic optimization design process of multistage axial turbine is presented in this article: first, applying quasi-three dimensional(Q3D) design methods to conduct preliminary design and then adopting modern optimization design methods to implement multistage local optimization. Quasi-three dimensional(Q3D) design methods, which mainly refer to S2 flow surface direct problem calculation, adopt the S2 flow surface direct problem calculation program of Harbin Institute of Technology. Multistage local optimization adopts the software of Numeca/Design3D, which jointly adopts genetic algorithm and artificial neural network. The major principle of the methodology is that the successive design evaluation is performed by using an artificial neural network instead of a flow solver and the genetic algorithms may be used in an efficient way. Flow computation applies three-dimensional viscosity Navier Stokes(N-S) equation solver. Such optimization process has three features: (i) local optimization based on aerodynamic performance of every cascade; (ii) several times of optimizations being performed to every cascade; and (iii) alternate use of coarse grid and fine grid. Such process was applied to optimize a three-stage axial turbine. During the optimization, blade shape and meridional channel were respectively optimized. Through optimization, the total efficiency increased 1.3% and total power increased 2.4% while total flow rate only slightly changed. Therefore, the total performance was improved and the design objective was achieved. The preliminary design makes use of quasi-three dimensional(Q3D) design methods to achieve most reasonable parameter distribution so as to preliminarily enhance total performance. Then total performance will be further improved by adopting multistage local optimization design. Thus the design objective will be successfully achieved without huge expenditure of manpower and calculation time. Therefore, such optimization design process may be efficiently applied to the aerodynamic design optimization of multistage axial turbine.

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