• 식물분류학회지
• /
• 제49권1호
• /
• pp.70-89
• /
• 2019

경기도 용인시에 위치하는 정광산의 관속식물상을 조사하고 이에 분포하는 주요식물을 확인하였다. 본 조사는 2015년 5월부터 2016년 9월까지 총 14회에 걸쳐 이루어졌으며, 조사 결과 정광산에서 총 89과 234속 323종 6아종 34변종 4품종의 367분류군의 관속식물 분포가 확인되었다. 이 중 한국특산식물은 8분류군이 확인되었고, 식물구계학적 특정식물은 IV등급 4분류군, III등급 4분류군, II등급 2분류군, I등급 9분류군으로 총 19분류군이 확인되었다. 국외반출 승인대상 식물은 59분류군이 확인되었다. 적색목록 식물로는 준위협종(Near Threatened, NT)으로 께묵 1분류군, 관심대상종(Least Concern, LC)으로 금붓꽃 1분류군으로 총 2분류군이 확인되었다. 귀화식물로는 개망초, 돼지풀, 붉은서나물 등을 포함하여 27분류군이 확인되었다.

• 한국산림과학회지
• /
• 제111권1호
• /
• pp.1-18
• /
• 2022

덕항산 산림유전자원보호구역(337 ha)은 강원도 삼척시 신기면에 위치하며, 고산지대에 발달한 우리나라 유일의 카르스트지형이다. 덕항산 산림유전자원보호구역에서 발견된 관속식물은 90과 302속 479종 4아종 51변종 3품종 총 537분류군으로 조사되었다. 그 중 한국특산식물은 북부 고산지대에 자생한다고 알려진 Pseudostellaria setulosa Ohwi(숲개별꽃)과 우리나라 석회암지대에서만 자생하는 Saussurea calcicola Nakai(사창분취)와 Peucedanum insolens Kitag.(덕우기름나물)를 포함한 19분류군이 발견되었고, 희귀 및 멸종위기식물은 멸종위기 야생식물 II급에 속하는 Astilboides tabularis(Hmsl.) Engl.(개병풍)과 Vioela websteri Hemsl.(왕제비꽃)을 포함한 17분류군의 분포를 확인하였다. 또한 석회암지대 지표식물 33분류군, 귀화식물은 27분류군이 확인되었다. 본 대상지는 온대북부 석회암지대의 특성상 특산식물과 북방계식물이 주로 발견되었으며, 남방계식물은 발견되지 않았다. 지구온난화로 인한 남방계 식물의 북상은 우려와는 달리 현재까지는 미비한 수준으로 판단되며, 덕항산은 북방계식물의 생육에 적합한 환경을 유지하고 있는 것으로 판단된다.

• 한국자원식물학회지
• /
• 제33권4호
• /
• pp.404-416
• /
• 2020

생물 다양성을 유지하고 기후 변화를 조절하는 데에 중심적인 역할을 하는 열대림에는 식품 또는 의약품 개발 등 산업적으로 이용 가능한 자원 식물이 풍부하다. 이들을 활용하기 위하여 열대 및 아열대 식물의 보존 및 증식에 관한 연구가 필요한데, 국내에서는 이에 관한 연구가 미비한 실정이다. 본 연구는 국립수목원 열대식물자원연구센터가 2013년부터 미얀마 천연자원 환경보전부(Ministry Of Natural Resources and Environmental Conservation)와 미얀마 산림과학원(Forest Research Institute)과 함께 미얀마 보호지역 및 Sagging 지역 내 식물 다양성 보존을 위한 공동 현장조사, 연구 및 출판 등의 협력사업을 진행하고 있는 정보를 제공하고자 한다. 국립수목원과 미얀마 산림과학원 공동연구의 성과로 2018년 12월 미얀마 산림과학원으로부터 미얀마 유용 및 관상식물 65과 326종의 식물 생체 및 표본 등을 기증받아 국립수목원 열대식물자원연구센터에 보존과 교육 목적으로 전시 중이다. 이 기증은 미얀마에서 식물 생체를 국외로 반출한 첫번째 사례이며, 국립수목원은 2008년 독일에서 열대식물을 기증받은 사례 다음으로 두번째로 외국 정부의 공식적인 대량 식물 도입 사례다. 도입한 식물 중 Santalum album L.은 IUCN Red List of Threatened Species (IUCN, 2017)에 취약종으로 분류되어 있다. 본 연구 결과를 토대로 열대 및 아열대 식물의 생육에 적합한 인공 환경을 구명하고 국내의 실내 정원소재 및 미세먼지, 공기정화 능력을 가진 열대식물의 발굴하고 추후 열대 및 아열대 식물의 보존 및 증식 방법과 더 나아가 생물 다양성 보존 및 산업적 이용을 위한 연구에 박차를 가할 수 있을 것이다.

• 한국환경생태학회지
• /
• 제29권2호
• /
• pp.105-131
• /
• 2015

This study was carried out to investigate the flora of Gyodongdo island (Ganghwa-gun). The vascular plants from 11 field surveys were revealed to belong to a total of 629 taxa; 118 families, 364 genera, 561 species, 5 subspecies, 53 varieties, 7 forms and 3 hybrids. 184 taxa were the first records from this region. The plants in Gyodongdo island are composed of the deciduous broad-leaved and conifer-mixed forests which are the common ones in the middle part of the Korean Peninsula. Five taxa of Korean endemic plants such as Clematis brachyura Maxim., Viola seoulensis Nakai, Populus ${\times}$ tomentiglandulosa T. B. Lee, Forsythia koreana (Rehder) Nakai and Hemerocallis hakuunensis Nakai were collected. Endangered wild plants designated by the law called 'Protection Law for Endangered wild fauna and flora' were one taxon. The red list plants according to IUCN valuation basis were examined for 13 taxa; endangered (EN) species of Prunus yedoensis Matsum., Vulnerable (VU) species of both Utricularia pilosa (Makino) Makino and Iris ruthenica var. nana Maxim., Near Threatened (NT) species of Senecio argunensis Turcz., Least Concern (LC) species of Platycladus orientalis (L.) Franco, Potentilla discolor Bunge, Limnophila sessiliflora (Vahl) Blume, Acorus calamus L., Phacelurus latifolius (Steud.) Ohwi, Pseudoraphis ukishiba Ohwi, Belamcanda chinensis (L.) DC., and Not Evaluate (NE) species of both Astragalus sikokianus Nakai and Potamogeton oxyphyllus Miq. The floristic regional indicator plants found in this area were a total of 47 taxa comprising three taxa of grade V, four taxa of grade IV, nine taxa of grade III, 10 taxa of grade II, and 21 taxa of grade I. The naturalized plants were identified as 62 taxa and the percentage of naturalized index (NI) was 9.9 % and the percentage of urbanization index (UI) was 19.3 %, respectively. Furthermore, hemicryptophytes (28 %), therophytes (26 %), hydrophytes (13 %) and geophyte (12 %) showed high proportional ratio in life form spectrum.

• Nuclear Engineering and Technology
• /
• 제54권10호
• /
• pp.3745-3765
• /
• 2022

The concrete structures related to nuclear safety are threatened by accidental impact loadings, mainly including the low-velocity drop-weight impact (e.g., spent fuel cask and assembly, etc. with the velocity less than 20 m/s) and high-speed projectile impact (e.g., steel pipe, valve, turbine bucket, etc. with the velocity higher than 20 m/s), while the existing studies are still limited in the impact resistant design of nuclear power plant (NPP), especially the primary RC slab. This paper aims to propose the numerical simulation and theoretical approaches to assist the impact-resistant design of RC slab in NPP. Firstly, the continuous surface cap (CSC) model parameters for concrete with the compressive strength of 20-70 MPa are fully calibrated and verified, and the refined numerical simulation approach is proposed. Secondly, the two-degree freedom (TDOF) model with considering the mutual effect of flexural and shear resistance of RC slab are developed. Furthermore, based on the low-velocity drop hammer tests and high-speed soft/hard projectile impact tests on RC slabs, the adopted numerical simulation and TDOF model approaches are fully validated by the flexural and punching shear damage, deflection, and impact force time-histories of RC slabs. Finally, as for the two low-velocity impact scenarios, the design procedure of RC slab based on TDOF model is validated and recommended. Meanwhile, as for the four actual high-speed impact scenarios, the impact-resistant design specification in Chinese code NB/T 20012-2019 is evaluated, the over conservation of which is found, and the proposed numerical approach is recommended. The present work could beneficially guide the impact-resistant design and safety assessment of NPPs against the accidental impact loadings.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.28-28
• /
• 2022

This paper presents the first genome assemblies of Philippine mangoes that provide valuable reference for varietal improvement and genomic studies on mango and related fruit crops. WE sequenced whole genomes of3 species, Mangifera odorata (Huani), Mangifera altissima (Paho), and Mangifera indica 'Carabao' (Sweet Elena). 'Carabao' is the major export variety of the Philippines; Paho is identified as vulnerable by the IUCN Red List of Threatened Species; Huani has fruit sap acrid which is the primary defense mechanism against insects and birds. We used Falcon, a diploid aware -de novo assembler to assemble SMRT generated long-read sequences. Falcon-unzip was employed to phase the output assembly producing larger contig sets (primary contigs) and shorter contigs corresponding to haplotypes (haplotigs). Assembly statistics were generated by comparing the assembly to a reference genome, Tommy Atkins, using Quality Assessment Tool (QUAST). Moreover, the extent of duplication and completeness of gene content was measured using Benchmarking Universal Single-Copy Orthologs (BUSCO). Draft assemblies with high duplications were processed using Purge Haplotigs and Purge Dups to lessen duplications with minimal impact on genome completeness. De novo assemblies of Huani, Paho and 'Carabao' were then generated with primary contig sizes of 463.64 Mb, 508.95 Mb and 401.51 Mb respectively. These draft assemblies of Huani, Paho and 'Carabao' showed 96.90%, 95.17% and 99.07% complete BUSCOs respectively which is comparable to 'Tommy Atkins' genome (98.6%). Using two mango transcriptome data (pooled RNA-seq from different mango varieties and tissues), 91-96% or 24-30 million reads were successfully mapped back for each generated assembly indicating high degree of completeness. The results obtained demonstrated the highly contiguous, phased, and near complete genome assembly of three Philippine mango species for structural and functional annotation of gene units, especially those with economic importance.

• Plant Biotechnology Reports
• /
• 제2권2호
• /
• pp.163-169
• /
• 2008

Roots of Ophiorrhiza prostrata D. Don serve as a rich source of camptothecin (CPT), an anticancer drug. Because of the large-scale collection of its roots, the plant has become a threatened species. The present study accomplishes the induction of adventitious roots as a means for the production of CPT as well as for the large-scale propagation of this anticancer drug plant using leaf and internode explants. The biomass yield and CPT content of adventitious roots induced from different explants were compared to roots developed on ex vitro rooted stem cuttings. Adventitious roots were produced on half-strength Murashige and Skoog (MS) medium supplemented with $10.74{\mu}M$ ${\alpha}-naphthaleneacetic$ acid and $2.32{\mu}M$ kinetin at mean fresh weights of 0.753, 0.739 and 0.748 g roots from leaf, internode and shoot, respectively. CPT yield from in vitro derived roots after 50, 80 and 120 days of incubation (0.028, 0.06 and 0.1% dry weight, respectively) was not significantly different from those harvested at the same age from ex vitro rooted (0.03, 0.06 and 0.13%, respectively) stem cuttings. CPT from subcultured roots derived from solid (0.08%) medium was lower than from suspension culture medium (0.12%). Subsequent cultures of the adventitious roots showed a stable production of CPT (0.16%). The yield of CPT from 360-day-old plant-derived roots was 0.19%. Elicitation using methyl jasmonate and acetyl salicylic acid exhibited no enhancement in CPT yield. In vitro propagation through direct shoot regeneration was achieved from the adventitious roots upon transfer to MS medium with $8.87{\mu}M$ $N^6-benzyladenine$ (BA) and $2.46{\mu}M$ indole-3-butyric acid (IBA) with a mean of 21.2 shoots per culture in 50 days. The shoots upon subculture on medium having the same level of BA and IBA underwent rapid proliferation. The shoots transferred to field conditions after in vitro rooting exhibited 95% survival. Adventitious root induction, from leaf and internode explants, enables the feasible production of CPT as well as the large-scale rapid propagation of this species which can safeguard it from extinction.

• 식물분류학회지
• /
• 제36권1호
• /
• pp.61-89
• /
• 2006

풍혈지는 여름철에 너덜지대 사면의 암괴 틈에서 찬 공기가 스며 나오며, 또한 결빙현상을 보이는 국소적 저온환경을 형성하는 지역이다. 본 연구에서는 풍혈지에 대한 식물상적 조사를 바탕으로 식물지리학적 중요성과 보존의 필요성을 제시하고자 국내 주요 풍혈지 7개소에 대한 현지 조사를 실시하였다. 그 결과, 풍혈지에서 한들고사리, 두메고사리 좀미역고사리, 월귤, 뚝지치 등 식물지리학적으로 중요한 다수의 북방계 식물들이 조사되었다. 또한 수직분포면에서도 산우드풀, 민둥인가목, 요강나물, 산새풀, 집사초와 같은 아고산식물들이 해발이 낮은 풍혈지에 생육하는 특이성을 보이고 있었다. 풍혈지는 빙하기에 남하했던 북방계 식물들이 최후빙기 이후 이주과정에서 국소적 기후환경에 적응, 고립되어 있는 피난처로 판단되어진다. 고피난처인 풍혈지는 기상학, 지형학적 중요성뿐만 아니라 식물지리학적으로도 아주 중요한 서식지 형태로 소집단으로 격리 분포하는 북방계 식물 종들에 대한 보존생물학적 연구를 통한 합리적인 현지내 외 보존대책의 수립이 시급하다고 판단된다.

• Journal of Microbiology and Biotechnology
• /
• 제20권3호
• /
• pp.630-636
• /
• 2010

A survey of the endangered orchid Orchis militaris populations was carried out in north-eastern Italy. The occurrence of fungal root endophytes was investigated by light and electron microscopies and molecular techniques. Two main sites of presence were individuated in the Euganean Hills, differing as to the percentage of flowering individuals and of capsules completing maturity. Fluorescence microscopy revealed an intracellular cortical colonization by hyphal pelotons. Two ITS PCR products co-amplified. Sequencing revealed for the former an identity and a high similarity (99%) with a Tulasnellaceae (Basidiomycota) fungus found within tissues of the same host in independent studies in Hungary and Estonia, suggesting an interesting case of tight specificity throughout the Eurosiberian home range. The second amplicon had 99% similarity with Tetracladium species (Ascomycota) recently demonstrated as potential endophytes. TEM revealed two different hyphal structures. Double fungal colonization appears to occur in Orchis militaris and the possible requirement of a specific fungal partner throws light on the causes of this plant's rarity and threatened status.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2007년도 학술발표회 논문집
• /
• pp.2039-2043
• /
• 2007

Like other major river basin systems in the West of the United States the Platte River Basin are faced with the challenges of allocating more water for plant and animal species. A part of the Central Platte River was designated as critical habitat for the whooping crane in 1978. The water allocation system in the Platte River Basin is dominated by the Prior Appropriation Doctrine, which allocates water according to the priorities based on the date of water use. The Platte River Basin segregated into five subregions for purpose of analysis. 24 years of historic records of monthly flow and all the demands were complied. The simulation of river basin modeling includes physical operation of the system including water allocation by water rights and interstate compact agreements, reservoir operations, and diversion with consumptive use and return flow. MODSIM, a generalized river basin network model, was used for estimating the timing and magnitude of impacts on river flows and diversions associated with water transfers from each region. A total of 20 alternatives were considered, covering transfers from each of the five regions of basin with several options. The result shows that the timing and availability of augmented water at the critical habitat is not only a function of use by junior appropriators, but also of river losses, and timing of return flows.