• 공업화학
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• 제1권2호
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• pp.249-255
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• 1990

Thin layer chromatography를 사용하여 음이온성 및 비이온성 계면활성제의 신속한 분리 및 정성분석에 필요한 용매조건들을 조사하였다. 알킬사슬의 종류와 alkylene oxide의 분포에 따른 분리특성을 이용하여 polyoxyalkylene 계 비이온성 계면활성제들을 간단히 정성 분석 할 수 있었으며, densitometer를 사용하여 polyoxyethylenated nonyl phenol의 ethylene oxide(EO)에 따른 분포를 쉽게 비교할 수 있었다. 일련의 음이온성 계면활성제들을 $R_f$ 값과 발색되는 색으로 정성분석하였으며, 음이온성 및 비이온성 계면활성제 혼합물을 각각 분리하였다. 지방산계 계면활성제를 diester, monoester 및 미반응된 polyethylene glycol의 세 부분으로 농축 분리하여 그 혼합비율을 구하였다. 이상의 모든 실험은 전개용매를 80mm정도 전개시킨 상태에서 이루어졌고 분리에 걸린 시간은 13-20분 정도였다.

• 생명과학회지
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• 제23권4호
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• pp.537-541
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• 2013

Biodiesel 중의 glycerol을 분석하기 위하여 TLC를 이용하여 몇 가지 보고된 이동상에서 glycerol을 분석하였다. 그 중에서 acetonitrile : distilled water (85:15, v/v)를 이동상으로 하여 TLC를 수행하였을 경우에 짧은 시간에 명확한 glycerol band를 확인할 수 있었다. X축의 glycerol 농도를 log scale로 하고, TLC의 glycerol 이미지 면적을 Y축으로 하여 3.0-0.0625 (%, w/v)의 glycerol 농도범위에서 표준곡선을 작성할 수 있었으며, 이를 이용하여 0.2 (%, w/v)의 glycerol을 포함하는 biodiesel 시료에서 유의성 있게 glycerol을 정량분석 할 수 있음을 확인하였다. 이러한 결과는 chemical assay, enzymatic assay를 이용한 glycerol 분석과 비교하여 매우 유사한 결과이며, TLC를 이용한 biodiesel 중의 glycerol 정량법은 특별한 분석기기를 사용할지 않아도 되는 편리하고 간편한 방법으로 생각되어진다.

• 대한화학회지
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• 제18권5호
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• pp.368-372
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• 1974

Bombina Orientalis(무당개구리)의 복피로부터 추출한 암적색의 색소를 TLC, PLC(preparative thin layer chromatography) 및 column chromatography로 분리 및 정제하여 물리, 화학적 성질 visible 및 IR-spectral 특성을 이용하여 세번째주색소인 ${\alpha}$-cryptoxanthin(3-hydroxy-${\alpha}$-carotene)을 확인하였다

• 한국환경보건학회지
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• 제2권1호
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• pp.5-9
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• 1975

For analysis of Xanthene dyes according to the developing solvent and adsorbent was applied to Thin-layer chromatography with silicagel and cellulose plate. Silicagel chromato-plate used were prepared under different condition of activation. Using eight developing solvent, the influence of the condition for activation upon the separation of Xanthene dyes was investigated. The results are shown in Table 3. Methyl ethyl ketone+Acetone+$H_2O$ (10:0.1:0.4) mixture and n-butanol+Ammonia water (4:1) mixture gave clear separation for Xanthene dyes, including Fluorescein, Erythrosine Rhodamin B, Eosine, Rose bengale, phloxine and Acid red those Rf values decrease in the described ordor. Methyl ethyl ketone+Acetone+$H_2O$ (10:0.1:0.4) was applied to two adsorbents which were purchased from different manufactures. The results of Chromatograms are obtained Figure 6.

• 약학회지
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• 제11권3_4호
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• pp.33-35
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• 1967

The internal structures of "Chunho", "Kangwhal" and "Dangui" and their original plants had been compared with each other in the prior report. Coumarin derivatives, khellactone from the roots of Angelica purpuraefolia $C_{HUNG}$ and decursin and decursinol from A. gigas $N_{AKAI}$ were also isolated. In this report, we made a simple and convenient identification method for the powdered drugs of several Angelica genus by using a thin layer chromatogrophic technique. The ehter extract of the powdered drugs were applied to a silica gel plate, and was developed with n-hexane and ethyl acetate (1:1) mixture. The characteristic U.V. absorption patterns of each drug were shown.

• 한국환경보건학회지
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• 제1권1호
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• pp.14-17
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• 1974

A study was carried out to detect of illegal artificial dyes, and to confirm the used rate of illegal dyes in the production process of commercial drops (candy), from Dec. 10, 1972 to Feb. 10, 1973. In this study, it was used thin layer chromatography, the samples were divided into two groups, group A of inscribed trade name and group B of not inscribed trade name. To contrast with group A and group B, 100 samples were randomly collected in the market places, 50 samples from group A and 50 samples from group B. The following conclusions were obtained: 1) used rate of illegal dyes were 2% of group A and 9% of group B. 2) used rate of illegal dyes were 2% of red, 2% of yellow, 1% of violet and 6% of green, not in blue. 3) used rate of illegal dyes 3% of Rhodamine B, 3% of Auramine and 6% of Light green S.F. Yellowish. 4) Out of 20 cases those used mixed dyes, only one case was confirmed as two illegal dyes, and 4 cases were used one illegal dye.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.230-239
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• 2022

Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α-naphthaleneacetic acid. In addition, preliminary phytochemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.

• 한국작물학회지
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• 제42권6호
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• pp.821-832
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• 1997

본 실험은 알파파 꽃 추출물로부터 Allelopathy와 Autotoxicity에 관련되는 활성물질을 분리, 동정하기 위하여 실시하였다. 알팔파 꽃 200g을 80% MeOH로 추출하여 $CHCl_3$층을 silica gel thin layer chromatography (TLC), microcrystalline cellulose thin layer chromatography (MCTLC)와 droplet countercurrent chromatography(DCCC)의 분획을 이용하여 생물검정을 행하고 DCCC의 생물검정에서 가장 억제력을 보였던 분획 5와 6은 HPLC로 활성물질을 동정하였다. HPLC분석 결과 분획 5에서는 ferulic acid, caffeic acid, vanillic acid, rutin, narringin 이, 분획 6에서는 ferulic acid, caffeic acid, vanillic acid, rutin, coumarin이 동정되었다. 이들 동정된 물질을 이용한 알팔파와 돌피에 대한 발아실험에서도 모두 알팔파와 돌피의 발아와 생육에 억제적으로 작용하였으며, 이중 coumarin의 처리가 가장 억제적이었다. 따라서 이들 물질이 알팔파와 돌피의 autotoxicity와 allelopathy에 관련하는 것으로 생각되었다.

• 약학회지
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• 제21권2호
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• pp.110-114
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• 1977

The purified .omicron.-iodobenzoic acid-$^{131}$ I, sodium iodide-$^{131}$I and mixtures of those compounds were applied to radio paper or radio thin layer chromatography to select the developing solvents with useful separation efficiency. The separation efficiencies were checked by radiochromatogram sacnnings. It has been found that the two dimensional radio paper chromatogrphy using the solvent systems of 8% phenol and butanol-glacial acid-water (4:1:1 v/v) and the radio thin layer chromatography using alumina gel plate and butanol- glacial acetic acid- water (4:1:1 v/v) are both efficient. In this method, the Rf value of .omicron.-iodobenzoic acid-$^{131}$ I and the unbound $^{131}$ I is 0.48, 0.85 and 0.15, respectively.

• Applied Biological Chemistry
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• 제23권4호
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• pp.242-245
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• 1980

Panax ginseng의 과피(果皮) anthocyanins 색소(色素)를 1% HCl/propanol로 추출하여 thin layer chromatography로 분리하였으며 이를 다시 paper chromatography로 검정하였다. 침건반응, spectral analysis 등의 방법으로 주색소(主色素)가 pelragonidin-3-monoglucoside임을 확인하였다. Panax quinquefolius의 색소도 같은 방법으로 동정하였으며 주색소(主色素)가 pelragonidin-3-monogluside임이 확인되었으며 Panax ginseng에 있는 5색소 Band중에서 제2색소(第二色素)(Band 4)가 결여되어 있었다.