• 한국균학회지
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• 제15권3호
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• pp.187-195
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• 1987

두가지 열처리법에 의한 균 분리방법을 사용하여, 보통균을 제외한 토양균의 분리와 그의 논토양중의 수직 및 계절별 분포를 파악하기 위하여 역곡동과 신원동의 논토양을 시료로 사용하였다. $42^{\circ}C$에서 2일간 배양후 $37^{\circ}C$로 배양하는 방법과 $70^{\circ}C$에서 15분간 열처리하는 방법을 사용하였다. $42^{\circ}C$ 배양법에서는 두 장소에서 분리된 토양균은 거의가 mesophile이었으며 thermotolerant fungi는 Asergillus fumigatus, thermophile로는 Sporotrichum thermophile과 Malbranchea pulchella var. sulfrea이었고, 이들 중 우점종은 Asergillus fumigatus이었다. 두 장소에서 9속, 14종이 분리동정되었으며 Sporotrichum thermophile, Talaromyces ucrainicus, Malbranchea pulchella var. sulfrea는 한국 미기록 종이다. $72^{\circ}C$ 열처리방법으로 분리한 경우 두 장소에서 10속, 20종이 분리동정되었으며, 분리된 균은 거의가 mesophile이었으며 thermotolerant fungi는 Asergillus fumigatus이었고, 우점종은 Talaromyces stipitatus이었다. 또한 Talaromyces helicus var. major, Emericella nidulans var. nidulans, Chaetomium subspirale, Neosartorya fishiri var. fischeri는 한국 미기록 종이다. 두가지 분리방법으로 분리된 토양균의 계절적 분포는 여름과 봄이 가을과 겨울보다 높았으며, 전체 균의 수와 우점종 균수, 그리고 나타나는 종의 빈도는 그 수직분포에 있어서 상층에서 가장 높았으나 하층으로 내려갈수록 감소하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제41권2호
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• pp.28-35
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• 2020

Entomopathogenic Beauveria bassiana and Metarhizium anisopliae are well-known biological control agents worldwide, and have high potential in industrialization. However, their thermo-susceptibility limits long-term storage under high temperature conditions and insecticidal activity after application in target pests. The virulences of M. anisopliae JEF-003 and JEF-004, and B. bassiana JEF-006 and JEF-007 against bean bug (Riptortus pedestris), and the thermotolerance of conidia produced on three kinds of grains, sorghum, millet and Italian millet as substrates for solid cultures were investigated. Of the three grains, Italian millet was the suitable grains in the production of thermotolerant conidia, and a significant relationship between conidial thermotolerance and the media was demonstrated. This work suggests that biological characteristics of entomopathogenic fungi are altered by culture media, and these results will be a chance to understand the factors in the media which make such changes.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.873-876
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• 1999

Aspergillus sp. CX-l strain grown on microcrystalline cellulose resulted in the accumulation of high levels of cellulase and xylanase activities that were higher by two to four folds than those from the conventional commercial producer, Trichoderma reesei QM9414. Aspergillus sp. CX-1 demonstrated greater thermo stability and better catalytic characteristics of total cellulase activity (FPase) as compared to T. reesei and Aspergillus niger F-2039.

• Mycobiology
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• 제33권2호
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• pp.90-96
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• 2005

Sixty two out of the sixty four species of fungal isolates tested could produce both $exo-{\beta}1,4-gluconase\;(C_1)$ and $endo-{\beta}1,4-gluconase\;(C_x)$ on pure cellulose and rice straw as carbon source in Czapek's medium. Fifty-eight and fifteen species were able to grow at $25^{\circ}C$ and at $45^{\circ}C$, respectively. Eleven species could grow at both $25^{\circ}C$ and $45^{\circ}C$ while, four species appeared only at $45^{\circ}C$. The most cellulolytic species at $25^{\circ}C$ was Trichoderma koningii producing 1.164 $C_1$ (mg glucose/1 ml culture filtrate/1 hr) and 2.690 $C_x$ on pure cellulose, and 0.889 $C_1$, and 1.810 $C_x$ on rice straw, respectively. At $45^{\circ}C$, the most active thermotolerant species were Aspergillus terreus, followed by A. fumigatus. Talaromyces thermophilus was the highest active thermophilic species followed by Malbranchea sulfurea. Most of these species were also active in fermentation of rice straw at 25 and $45^{\circ}C$ (P<0.05). The most active ones were T. koningii, A. ochraceus and A. terreus, which produced 201.5, 193.1 and 188.1 mg crude protein/g dry straw, respectively.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1088-1095
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• 2014

A systematic investigation was performed on the bacterial, Bacillus, fungal, and yeast communities of the three types of Daqu (mechanically prepared, manually prepared, and mixed prepared) used in Baiyunbian Company by reconditioning PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The DGGE results showed that the microbes in the three types of Daqu were mainly thermotolerant and thermophilic microbes, and the most dominant bacterial species were Bacillus and Virgibacillus, followed by Lactobacillus and Trichococcus. Furthermore, the dominant fungi were found to be molds, such as Rasamsonia, Penicillium, Aspergillus, and Monascus, and the dominant yeasts were Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Pichia anomala, and Debaryomyces hansenii. In general, the three types of Daqu showed slight differences in microbial communities, and the Shannon indexes (H') of the manually prepared and mechanically prepared Daqu were similar. The results suggest that mechanically prepared Daqu can replace manually prepared Daqu in liquor production, and this research provides useful information for liquor production and process improvement.