• Fisheries and Aquatic Sciences
• /
• 제24권7호
• /
• pp.235-242
• /
• 2021

The present study investigated the critical thermal maxima (CT_max) of red-spotted grouper, Epinephelus akaara under different acclimation temperatures (T_acc). Fish were acclimated at 24℃, 28℃, and 32℃ water temperature for 2 weeks. Water temperature was increased at a rate of 1℃/h and CT_max level was measured following the critical thermal methodology (Paladino et al., 1980). The results showed that CT_max values of E. akaara were 35.61℃, 36.83℃, and 37.65℃ for fish acclimated at 24℃, 28℃, and 32℃, respectively. The acclimation response ratio (ARR) was 0.26. The CT_max values were significantly correlated with body size. Collectively, it is said that the CT_max value of red-spotted grouper can be affected by different adaptation temperature (24℃, 28℃, and 32℃) and the fish acclimated to a higher temperature has a higher CT_max level. Besides, the CT_max value of 35.61℃-37.65℃ indicating the upper thermal tolerance limit for E. akaara under different T_acc (24℃, 28℃, and 32℃). Understanding the thermal tolerance of E. akaara is of ecological importance in the conservation of this species.

• Animal cells and systems
• /
• 제3권1호
• /
• pp.47-52
• /
• 1999

Change of proteins was confirmed during low temperature acclimation of overwintering larva, and some biochemical characteristics of the induced antifreeze protein-1 (AFP-1) were investigated in Protaetia brevitarsis. As the freezing point depression by the action of induced AFPs, a considerable thermal hysteresis was observed in the haemolymph and in partially purified proteins. AFP-1 was purified from the cold acclimation larvae by ammonium sulfate precipitation ion exchange chromatography, gel permeation chromatography, and electroelution. The purified AFP-1 was determined to be a glycoprotein (approximately 320 kDa, pl 5.8) composed of a single type of subunit (80 kDa). The high contents of hydrophilic amino acids (Asp, Glu, Lys, Asn, Gln, Arg, Ser, Thr) were also confirmed, showing similarity with antifreeze proteins from other insects.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권7호
• /
• pp.1033-1039
• /
• 2006

The metabolic response of Labeo rohita to thermal acclimation was assessed. Advanced fingerlings of L. rohita (average weight $31{\pm}1.4g$) were acclimated to 31, 33 and $36^{\circ}C$ compared with ambient temperatures ($26^{\circ}C$) for 30 days and different enzymes associated with stress response were estimated. Glycolytic enzyme-Lactate dehydrogenase, (LDH, E.C.1.1.1.27), TCA cycle enzyme-Malate dehydrogenase (MDH, E.C.1.1.1.37), Protein metabolizing enzymes-Aspartate amino transferase (AST, E.C.2.6.1.1) and Alanine amino transferase (ALT, E.C.2.6.1.2) of liver, gill and muscle, Gluconeogenic enzymes-Fructose 1,6 Bi phosphatase (FBPase, E.C. 3.1.3.11) and Glucose 6 phosphatase (G6Pase, E.C. 3.1.3.9) of liver and kidney were significantly (p<0.05) different with increasing acclimation temperatures. Heat Shock Protein-70 (HSP-70) was expressed in increasing intensity at 31, 33 and $36^{\circ}C$ but was not expressed at $26^{\circ}C$. Results suggest that higher acclimation temperatures enhance metabolism and L. rohita maintains homeostasis between $26-36^{\circ}C$ via an acclimation episode. Such adaptation appears to be facilitated by resorting to gluconeogenic and glycogenolytic pathways for energy mobilization and induction of HSPs.

• Journal of Microbiology and Biotechnology
• /
• 제24권2호
• /
• pp.264-269
• /
• 2014

For the production of ethanol from seaweed as the source material, thermal acid hydrolysis and enzymatic saccharification were carried out for monosugars production of 25.5 g/l galactose and 7.6 g/l glucose using Gelidium amansii. The fermentation was performed with Pichia stipitis KCTC 7228 or Saccharomyces cerevisiae KCCM 1129. When wild P. stipitis and S. cerevisiae were used, the ethanol productions of 11.2 g/l and 6.9 g/l were produced, respectively. The ethanol productions of 16.6 g/l and 14.6 g/l were produced using P. stipitis and S. cerevisiae acclimated to high concentration of galactose, respectively. The yields of ethanol fermentation increased to 0.5 and 0.44 from 0.34 and 0.21 using acclimated P. stipitis and S. cerevisiae, respectively. Therefore, acclimation of yeasts to a specific sugar such as galactose reduced the glucose-induced repression on the transport of galactose.

• 한국잠사곤충학회지
• /
• 제39권1호
• /
• pp.1-11
• /
• 1997

In relations to cold acclimation, experiment was carried out to understand the seasonal changes in reserve substances of the mulberry. The shoot barks and leaves of three mulberry varieties(Kaeryangppong, Shinilppong and Yongcheonppong) were sampled, after that their reserve substances were analyzed. The cold hardiness of mulberry was investigated by DTA(Differential Thermal Analysis) method. To increase cold hardiness, gibberellin(100 ppm), kinetin(100 ppm) and Jambi 8 were sprayed on the mulberry leaves. After spraying, falling of the leaves of Yongcheonppong occured earier than the other varities. After the first frost, all of treatments except gibberellin were entirely fallen. Growth regulator extended the leaves fallen. After spraying, water of the shoot barks was not showed difference in the content among the treatments, but amino acid, carbohydrate and soluble protein increased from September to October. Starch content of the shoot barks and leaves was maximum in October, but thereafter decreased during wintering stage. In Shinilppong, Jambi 8 spray increased cold hardiness by 1-2$^{\circ}C$ more than no spray. It was concluded that the cold hardiness of the mulberry in midwinter is closely related to the reserve substances with spraying Jambi 8 on the mulberry leaves.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제4권1호
• /
• pp.13-17
• /
• 2002

Present communication deals with quantitative determination of total lipid, triglycerides, total free fatty acids, phospholipids and total cholesterol in the fat body tissue of the silkworm adapted to low and high temperatures. At the end of spinning process is characterized by a marked cellular reorganization of the different lipid fraction of the fat body irrespective of thermal acclimation. Accordingly, the per cent composition of triglycerides of the total lipid is increased accompanied by a corresponding decrease in free fatty acids, phospholipids and cholesterol.

• Animal cells and systems
• /
• 제14권1호
• /
• pp.17-23
• /
• 2010

We isolated warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of olive flounder and investigated the mRNA expression of Wap65 and HSP70 in olive flounder exposed to osmotic (17.5, 8.75, and 4 psu) and thermal stress (25 and $30^{\circ}C$). The mRNA expression of Wap65 and HSP70 was increased by thermal stress. The mRNA expression of HSP70 was also increased by osmotic stress, whereas no significant change in Wap65 expression was detected. These results indicate that Wap65 mRNA expression occurs specifically in response to increases in water temperature, but not in response to osmotic stress. Plasma cortisol levels were also increased by osmotic and thermal stress. We also utilized the stress hormone cortisol to examine whether Wap65 expression is thermal-stress-specific. Cortisol treatment increased HSP70 mRNA expression in vitro, but had no significant effect on Wap65 mRNA expression. Thus, thermal stress, but not osmotic stress, induces Wap65 expression.

• Fisheries and Aquatic Sciences
• /
• 제6권2호
• /
• pp.59-65
• /
• 2003

The critical thermal maximum (CTM) of black rockfish, Sebastes schlegeli, was evaluated. Black rockfish were acclimated at $24^{\circ}C$, and then exposed to temperatures from 24 to $33^{\circ}C$. Black rockfish were kept in constant darkness and subjected to a gradual temperature increase $(1 ^{\circ}C\;12^{-1})$. The oxygen consumption rate (OCR) was measured using an automatic intermittent­flow-respirometer (AIFR) during the exposure period (from 119.3 to 143.5 h). The OCR increased from 94.5 to 214.2mL $O_2 kg^{-1}\;ww\;h^{-1}$ as the temperature rose from 24 to $29.4-30.9^{\circ}C$. Subsequently, the OCR increased abruptly, reaching 245.8-412.7mL $O_2 kg^{-1}\;ww\;h^{-1}$ at $32^{\circ}C$. This study suggests that the CTM for black rockfish is $29.4-30.9^{\circ}C$ when temperature is increased at $1^{\circ}C\;12h^{-1}$ following acclimation at $24^{\circ}C$.

• 한국발생생물학회지:발생과생식
• /
• 제23권4호
• /
• pp.345-353
• /
• 2019

Higher thermal exposure can influence the blood cell morphology and count. Hence, based on the previous results (Rahman et al., 2019), the present study evaluated the morphometric indices of erythrocytes and their nucleus under different water temperatures (25℃, 28℃, 31℃, and 34℃) to investigate their use as an indicator of thermal stress in red spotted grouper, Epinephelus akaara. 180 healthy specimens of E. akaara were exposed to four temperature conditions (25℃ as control, 28℃, 31℃, and 34℃) for 42 days, following 2 weeks of acclimation at 25℃. Erythrocyte major axis (EL), erythrocyte minor axis (EW), nucleus major axis (NL), and nucleus minor axis (NW) were examined from the blood smears on each sampling day (i.e., 2, 7, and 42 days of thermal exposure). EL and NL were significantly decreased, whereas EW and NW were increased at higher water temperature (31℃ and 34℃). The major-minor axis proportions of erythrocytes and their nucleus (EL/EW; NL/NW) were decreased with increasing water temperature (31℃ and 34℃). The strong relationships were observed among the morphometric indices of erythrocytes and their nucleus, especially in EL vs. NL and EW vs. NW. This study reveals that elevated water temperature (31℃ and 34℃) can influence the major and minor axis morphometry of erythrocytes and their nucleus in red spotted grouper. These indices may be used as stress indicators to monitor the health status of E. akaara and probably for other fish species.

• 한국동물학회지
• /
• 제29권2호
• /
• pp.107-120
• /
• 1986

한냉환경에 Sprague-Dawley계 웅성흰쥐를 순화시켜 SLDH, SLDH 동위효소, SALP, SALP 동위효소 및 SCPK 등의 활성치의 보상 변화에 따른 변화를 관찰 검토하였다. 한냉순화는 $4\\pm1^\\circC$(상대습도 70$\\sim$80%)에서 안정상태로 36일간 순화처리 하였으며 SLDH의 활성치는 한냉순화 초기에 유의성 있게 증가하였으나 곧 감소되어 정상치로 접근하며 36일구 까지 항정상태를 지속하였으며, 대조군의 SLDH 동위효소는 주로 3개의 전기영동분획 즉 $LDH_1, LDH_2, LDH_4 및 LDH_5$를 나타냈으나 한냉순화 기간중에는 3개의 분획 즉 $LDH_1, LDH_2 및 LDH_3$가 나타났으며, 한냉순화 시의 SLDH의 상승은 $SLDH_1, SLDH_2 및 SLDH_3$의 반영이라고 생각되며 SALP의 활성치는 한냉순화 초기에 유의적으로 증가하였으나 곧 감소되어 정상치로 접근하여 36일구까지 항정상태를 지속하였고, 대조군의 SALP동위효소는 2개의 전기영동분획을 지속하고 있으며, 한냉순화군 초기의 SALP 활성치의 증가는 $SALP_1$ 분획의 즉각적인 증가의 반영임을 알 수 있고, 한냉순화로 인하여 SCPK의 활성치는 순화초기에 유의적으로 상승하였으나 곧이어 안정치로 접근하여 순화 말기까지 항정상태를 지속하였다. 한냉순화$(4\\pm1^\\circC)$처리는 한냉 초기에 SLDH, SLDH 동위효소, SALP, SALP 동위효소 및 SCPK 활성치 모두에 영향을 끼치는 것으로 생각되며 따라서 상기 각종 효소가 직접 또는 간접적으로 열적조절에 관여하는 것으로 생각된다.