• Applied Biological Chemistry
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• v.36 no.1
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• pp.45-50
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• 1993

A psychrotrophic microorganism isolated from Alaska pollack (Theragra chalcoramma) produced soymilk-clotting enzyme(s) with relatively low proteolytic activity. The isolate No. 268 was tentatively identified as Pseudomonas sp. Soymilk-clotting activity of the crude enzyme solution was observed at temperatures ranging from 20 to $60^{\circ}C$ and the optimum temperature was $40^{\circ}C$. When the crude enzyme solution was preincubated for 30 minutes, the clotting activity was stable at temperatures up to $30^{\circ}C$ and 75% of the activity was retained at $40^{\circ}C$. The clotting activity was decreased as the pH of soymilk was increased from 5.8 to 7.3.